Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,120,361 G→A 5.0% R76C (CGC→TGC)  solA ← N‑methyltryptophan oxidase, FAD‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,120,3610GA 5.0% 254.9 / 2.6 79R76C (CGC→TGC) solAN‑methyltryptophan oxidase, FAD‑binding
Reads supporting (aligned to +/- strand):  ref base G (35/40);  new base A (2/2);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

GGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGCTGCCGTGTTGATGCGGTGGCATATGGGCGTCGGTCATTAGCACGTTT  >  NC_000913/1120214‑1120509
                                                                                                                                                   |                                                                                                                                                    
gggCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCgg                                                                                                                                                     <  4:30767/149‑1 (MQ=255)
gggCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCgg                                                                                                                                                     <  5:342821/149‑1 (MQ=255)
  gCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCgg                                                                                                                                                     >  5:226398/1‑147 (MQ=255)
    cATAATACCTTTCGACTCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGgaga                                                                                                                                                 <  2:101740/149‑1 (MQ=255)
       aaTCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGtt                                                                                                                                              >  3:74446/1‑149 (MQ=255)
           ccTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATc                                                                                                                                          >  3:237840/1‑149 (MQ=255)
            cTTGCGCATCGAGCTTTTCAACTTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGGGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGTGAGAGTTCATcc                                                                                                                                         >  8:192046/1‑149 (MQ=255)
            cTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCCGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATcc                                                                                                                                         >  8:254996/1‑149 (MQ=255)
            cTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATcc                                                                                                                                         >  4:320717/1‑149 (MQ=255)
             ttGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATcc                                                                                                                                         >  3:390180/1‑148 (MQ=255)
             ttGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATcc                                                                                                                                         >  1:130878/1‑148 (MQ=255)
               gcgcATCGAGCTTTTCAACGTGGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGGGCATCCca                                                                                                                                       <  6:164056/148‑1 (MQ=255)
                cgcATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCcaca                                                                                                                                     <  8:111947/149‑1 (MQ=255)
                cgcATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGCGAGAGTTCATCCcaca                                                                                                                                     <  1:16581/149‑1 (MQ=255)
                   aTCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATcagatctg                                                                                                                                   >  3:258902/1‑141 (MQ=255)
                   aTCGAGCTTTGCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGGTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCAt                                                                                                                                           <  4:258902/140‑1 (MQ=255)
                        gCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTgc                                                                                                                             <  4:289430/149‑1 (MQ=255)
                        gCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTgc                                                                                                                             <  6:73884/149‑1 (MQ=255)
                           tttCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGACAGTTCATCCCACAGCGTTTgcgcg                                                                                                                          >  2:300716/1‑149 (MQ=255)
                               aaCGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGTGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCgga                                                                                                                      <  3:368087/149‑1 (MQ=255)
                               aaCGTTGAGTTGCCATTGTTATGCGCTGTGGGCGACGTTGGCGAGAAATGTTTAATCAGCCGGGCCAAGTGTAAAGACTCCAGATGGTACAAAAATGGGATCTTCTTCGATGTGGAGGGAGAGTTACTCCCAAAGCGTAAgcgcgag                                                                                                                        >  5:55035/1‑145 (MQ=255)
                                aCGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCggag                                                                                                                     >  5:88624/1‑149 (MQ=255)
                                  gTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCggagga                                                                                                                   >  8:9776/1‑149 (MQ=255)
                                  gTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCggagga                                                                                                                   >  2:261188/1‑149 (MQ=255)
                                     gAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGAcc                                                                                                                 <  6:226398/148‑1 (MQ=255)
                                           ccATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGa                                                                                                          <  7:9776/149‑1 (MQ=255)
                                           ccATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGa                                                                                                          >  4:95008/1‑149 (MQ=255)
                                           ccATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGGTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCggg                                                                                                           <  3:312340/148‑1 (MQ=255)
                                           ccATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCAGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGa                                                                                                          >  2:193638/1‑149 (MQ=255)
                                           ccATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCAGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGa                                                                                                          >  2:187791/1‑149 (MQ=255)
                                            cATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGa                                                                                                          >  5:210038/1‑148 (MQ=255)
                                             aTTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACa                                                                                                        >  6:14064/1‑149 (MQ=255)
                                                 ttCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACt                                                                                                    >  7:34511/1‑149 (MQ=255)
                                                 ttCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACt                                                                                                    >  7:417125/1‑149 (MQ=255)
                                                 atCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGGTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACt                                                                                                    <  2:319222/148‑1 (MQ=255)
                                                   cGGCGCTGTGGGCGACGTTGGCGAGATATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACtt                                                                                                   <  3:171971/148‑1 (MQ=255)
                                                    ggCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACtttt                                                                                                 >  2:225355/1‑149 (MQ=255)
                                                     gcgcTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTc                                                                                                <  1:261188/149‑1 (MQ=255)
                                                           tgGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCt                                                                                            <  6:210038/147‑1 (MQ=255)
                                                           tgGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTc                                                                                          <  4:390180/149‑1 (MQ=255)
                                                             ggCTACGTTGGCGAGTAATGTGGAATCAGCCTGTCCATTGTTAATGACGCCAGAGCTTACATAAAGGGGATCTTCTTCGTTGTGGCGGGTGAGTTCATCCCACAGCTTTTGCGCGCGGAGGACCAGAGGGACATACTTTTCGCCTTCaa                                                                                        <  6:55035/149‑2 (MQ=255)
                                                                gACGTTGGCGAGAAATGTGCAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCAt                                                                                     <  6:170292/149‑1 (MQ=255)
                                                                  cGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATaa                                                                                   <  6:88624/149‑1 (MQ=255)
                                                                  cGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATaa                                                                                   <  3:320717/149‑1 (MQ=255)
                                                                    ttGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAg                                                                                  >  6:242101/1‑148 (MQ=255)
                                                                    ttGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGc                                                                                 <  7:356717/149‑1 (MQ=255)
                                                                    ttGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGc                                                                                 <  6:65496/149‑1 (MQ=255)
                                                                    ttGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGc                                                                                 <  1:251454/149‑1 (MQ=255)
                                                                      ggCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCa                                                                                <  5:242101/148‑1 (MQ=255)
                                                                       gCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACACCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATg                                                                              >  7:381950/1‑149 (MQ=255)
                                                                            aaaTGTGGAACCAGCCGGGCCAAGGTTAATGACGCCAGAGCGGACAAACATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCTCCGCGTTTGCGCGCGGAGGACCAGCGGGAGATAGTTTTCGCCTTCACCATAAGCATGGCGaa                                                                         <  2:384462/149‑1 (MQ=255)
                                                                                  ggAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATc                                                                   >  1:342122/1‑149 (MQ=255)
                                                                                    aaTCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATc                                                                   >  1:156724/1‑147 (MQ=255)
                                                                                    aaTCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATc                                                                   <  2:156724/147‑1 (MQ=255)
                                                                                      tCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCgga                                                                                                                      <  4:45679/94‑1 (MQ=255)
                                                                                      tCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCgga                                                                                                                      >  3:45679/1‑94 (MQ=255)
                                                                                         gCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATc                                                                   >  4:349141/1‑142 (MQ=255)
                                                                                         gCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATc                                                                   <  3:349141/142‑1 (MQ=255)
                                                                                          ccGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCg                                                           >  3:381543/1‑149 (MQ=255)
                                                                                                cAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCgtgg                                                     >  5:238868/1‑149 (MQ=255)
                                                                                                cAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCgtgg                                                     <  8:34511/149‑1 (MQ=255)
                                                                                                   ggTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGtggtgg                                                  >  2:112394/1‑149 (MQ=255)
                                                                                                   ggTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGtggtgg                                                  <  1:112394/149‑1 (MQ=255)
                                                                                                    gTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGc                                                 <  5:53917/149‑1 (MQ=255)
                                                                                                    gTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGc                                                 <  8:54429/149‑1 (MQ=255)
                                                                                                    gTTAATGACGAGAGTGCGTAGAAAAATTGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACTTCTTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCAGCATAAGGATGGCGAATTAATCGCGTATCGCCGTTGTGGc                                                 <  7:87974/149‑1 (MQ=255)
                                                                                                            cGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGCTGCCgtgt                                         <  2:183844/149‑1 (MQ=255)
                                                                                                            cGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCAGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGCTGCCgtgt                                         <  1:187791/149‑1 (MQ=255)
                                                                                                            cGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCAGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGCTGCCgtgt                                         <  1:193638/149‑1 (MQ=255)
                                                                                                             gCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGCTGCCGTGtt                                        <  5:14064/149‑1 (MQ=255)
                                                                                                               cAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCTCGCGGAGGACCAGCGGGCCATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGCTGCCGTGTTGa                                      >  1:146461/1‑149 (MQ=255)
                                                                                                               cAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGCTGCCGTGTTGa                                      >  2:314905/1‑149 (MQ=255)
                                                                                                                   tcGTACATATATGGGATCTTCTTCGTTGTGGGGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGTCATACTTTTCGCCTTCACCATTAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGCTGCCGTGTTGATGCg                                  <  2:146461/148‑1 (MQ=255)
                                                                                                                   gCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCAACAGCGTTTGCGCGCGGAGGCCCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGCTGCCGTGTTGATGCg                                  >  8:126661/1‑149 (MQ=255)
                                                                                                                       aCAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGCTGCCGTGTTGATGCGGTgg                              >  1:394329/1‑149 (MQ=255)
                                                                                                                         aaaaaTGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGCTGCCGTGTTGATGCGGTGGCa                            >  5:178647/1‑149 (MQ=255)
                                                                                                                                ggATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGCTGCCGTGTTGATGCGGTGGCATATGGGc                     <  2:342122/149‑1 (MQ=255)
                                                                                                                                     ttcttcGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGCTGCCGTGTTGATGCGGTGGCATATGGGCTTCgg                >  8:112209/1‑149 (MQ=255)
                                                                                                                                       cttcGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGCTGCCGTGTTGATGCGGTGGCATATGGGCGTCgg                <  4:381543/147‑1 (MQ=255)
                                                                                                                                        ttcGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGCTGCCGTGTTGATGCGGTGGCATATGGGCGTCGGTCa             >  7:386389/1‑149 (MQ=255)
                                                                                                                                                 gCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGCTGCCGTGTTGATGCGGTGGCATATGGGCGTCGGTCATTAGCACGt    <  8:417125/149‑1 (MQ=255)
                                                                                                                                                  cGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGCTGCCGTGTTGATGCGGTGGCATATGGGCGGCGGTCATTAGCACGtt   <  2:130878/149‑1 (MQ=255)
                                                                                                                                                   gggAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGCTGCCGTGTTGATGCGGTGGCATATGGGCGTCGGTCATTAGCACGttt  <  6:178647/149‑1 (MQ=255)
                                                                                                                                                   gggAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGCTGCCGTGTTGATGCGGTGGCATATGGGCGTCGGTCATTAGCACGtt   >  5:48908/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGGGAGAGTTCATCCCACAGCGTTTGCGCGCGGAGGACCAGCGGGACATACTTTTCGCCTTCACCATAAGCATGGCGAATTAATCGCGTATCGCCGTGGTGGCTGCCGTGTTGATGCGGTGGCATATGGGCGTCGGTCATTAGCACGTTT  >  NC_000913/1120214‑1120509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: