Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,729,865	C→T	5.9%	S260L (TCG→TTG)	lhr →	putative ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,729,865	0	C	T	5.9%	216.8 / 2.5	68	S260L (TCG→TTG)	lhr	putative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base C (26/38);  new base T (2/2);  total (28/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

ATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACG  >  NC_000913/1729717‑1730013                                                                                                                                                     |                                                                                                                                                     aTTGGCCTTTCTGCCACTGTGCTCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCAGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGACGTACCGGTCGCAAATAAGGATGATGTCTCATc                                                                                                                                                      >  7:168669/1‑149 (MQ=255) aTTGGACTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATc                                                                                                                                                      >  3:367726/1‑149 (MQ=255)     gCCTTTCTGCCTCTGTGCGCTCAGCCAGCGATGTGGCATCATTTCTTTGTGGCCATCGCCCGGGTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCCGTCGCCAATATGGATGATGTCTCATCGGTc                                                                                                                                                  <  4:260138/149‑1 (MQ=255)     gCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTc                                                                                                                                                  <  1:355048/149‑1 (MQ=255)     gCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTc                                                                                                                                                  >  6:24843/1‑149 (MQ=255)     gCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGGGGCAGCTTTTCTTTGTGGCGATCGCCCGGTTAGGGTAGTCTACCCGCCCGCAATGCGCCATCCGCAGATTCGAATTGTCGTACCGGGCGCCAATATGGATGATGTCTCATCGGTc                                                                                                                                                  <  4:254887/149‑1 (MQ=255)      ccTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCg                                                                                                                                                 <  7:100908/149‑1 (MQ=255)           cTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAg                                                                                                                                             <  5:244077/148‑1 (MQ=255)              ccACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGc                                                                                                                                            <  7:422445/146‑1 (MQ=255)              ccACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCgg                                                                                                                                          >  3:382814/1‑148 (MQ=255)              ccACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGc                                                                                                                                         >  4:184410/1‑149 (MQ=255)                    tgCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCgg                                                                                                                                    <  3:201307/148‑1 (MQ=255)                    tgCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCg                                                                                                                                     <  4:125133/147‑1 (MQ=255)                       gcTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGaa                                                                                                                                <  3:239326/149‑1 (MQ=255)                               agcGATGTGGCAGCATTTCTTGGTGGCGATCGCCAGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCa                                                                                                                        <  2:113214/149‑1 (MQ=255)                                gcGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCAt                                                                                                                       <  4:394141/149‑1 (MQ=255)                                        gcagcaTTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATTGGTCGCCAGCGGCACCGGCGAAGACAGCCATgccggccg                                                                                                               >  6:18848/1‑149 (MQ=255)                                        gcagcaTTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATTGGTCGCCAGCGGCACCGGCGAAGACAGCCATgccggccg                                                                                                               >  6:24470/1‑149 (MQ=255)                                             aTTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGa                                                                                                                              <  7:322043/129‑1 (MQ=255)                                             aTTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGa                                                                                                                              >  8:322043/1‑129 (MQ=255)                                              tttCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAgg                                                                                                         <  8:170776/149‑1 (MQ=255)                                                 cTTGGGGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCt                                                                                                       <  4:300559/148‑1 (MQ=255)                                                    ggtggCGATCGCCCGTGTACGGTAGGCAACCCGCCCGAAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATTATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTc                                                                                                      <  6:407621/146‑1 (MQ=255)                                                    ggtggCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGTATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTc                                                                                                      <  6:22784/146‑1 (MQ=255)                                                     gtggCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCACCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCAt                                                                                                   <  2:12945/148‑1 (MQ=255)                                                         cGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGc                                                                                              <  3:184410/149‑1 (MQ=255)                                                          gATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGc                                                                                              >  5:172123/1‑148 (MQ=255)                                                           aTCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCa                                                                                            >  4:90761/1‑149 (MQ=255)                                                            tCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCa                                                                                            >  4:257480/1‑148 (MQ=255)                                                              gCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCata                                                                                          >  6:340154/1‑148 (MQ=255)                                                                ccGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGCTGTCTCATCGGTCGCCAGCGGCACCGGCGCAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCatatat                                                                                       >  3:322869/1‑149 (MQ=255)                                                                ccGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCatatat                                                                                       <  4:382814/149‑1 (MQ=255)                                                                 cGGTTACGGTAGTCAACGCGCCCGCAATGCGCCATCCGCAGATACGTATTGTCGTACCGGGCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATAtt                                                                                      <  8:168669/149‑1 (MQ=255)                                                                       cGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAAc                                                                                 <  6:197139/148‑1 (MQ=255)                                                                       cGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACg                                                                                <  5:277526/149‑1 (MQ=255)                                                                        ggTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCATTATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTTGCCATATATTGAAACgg                                                                               <  6:107116/149‑1 (MQ=255)                                                                        ggTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACgg                                                                               <  5:33676/149‑1 (MQ=255)                                                                        ggTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACgg                                                                               <  2:214232/149‑1 (MQ=255)                                                                          tAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCCTCTGGCCATATATTGAAACGGGt                                                                             >  2:324538/1‑149 (MQ=255)                                                                             tCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATc                                                                          >  4:49861/1‑149 (MQ=255)                                                                                aCCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATTGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCtt                                                                       <  5:24470/149‑1 (MQ=255)                                                                                aCCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGGCGCCAATATGGATGATGTCTCATTGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCtt                                                                       <  5:18848/149‑1 (MQ=255)                                                                                  ccgcccgcAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTtga                                                                     >  4:88541/1‑149 (MQ=255)                                                                                     cccgcAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTtgatg                                                                   >  6:173371/1‑148 (MQ=255)                                                                                          aaTGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAgtgt                                                             >  2:399461/1‑149 (MQ=255)                                                                                              cgcCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCAGCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTgc                                                          <  5:24843/148‑1 (MQ=255)                                                                                                   tCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATc                                                    >  7:207437/1‑149 (MQ=255)                                                                                                   tCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATc                                                    <  3:120312/149‑1 (MQ=255)                                                                                                      gCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCt                                                 >  7:200623/1‑149 (MQ=255)                                                                                                         gATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGa                                              >  8:56787/1‑149 (MQ=255)                                                                                                          aTACGAATTGTCGTACCGCTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCAAATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGAc                                             >  8:153814/1‑149 (MQ=255)                                                                                                           tACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGAcc                                            <  6:172123/149‑1 (MQ=255)                                                                                                              gAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCatat                                                                                         <  8:56101/101‑1 (MQ=255)                                                                                                              gAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCatat                                                                                         >  7:56101/1‑101 (MQ=255)                                                                                                              gAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCAtt                                         <  3:91834/149‑1 (MQ=255)                                                                                                              gAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCAtt                                         <  1:399461/149‑1 (MQ=255)                                                                                                                    tCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAg                                                                <  1:62582/120‑1 (MQ=255)                                                                                                                    tCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAg                                                                >  2:62582/1‑120 (MQ=255)                                                                                                                      gTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCATCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTa                                  <  5:173371/148‑1 (MQ=255)                                                                                                                      gTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTAc                                 <  2:35092/149‑1 (MQ=255)                                                                                                                       tACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCtt                                                                       >  2:306827/1‑110 (MQ=255)                                                                                                                       tACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCtt                                                                       <  1:306827/110‑1 (MQ=255)                                                                                                                          cGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAAt                             >  3:390790/1‑149 (MQ=255)                                                                                                                            gTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCAGTGTCTTTACTAATTc                           >  6:135963/1‑149 (MQ=255)                                                                                                                                 cAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCggg                      >  7:382231/1‑149 (MQ=255)                                                                                                                                       ggATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTcgc                         >  5:386611/1‑140 (MQ=255)                                                                                                                                       ggATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTcgc                         <  6:386611/140‑1 (MQ=255)                                                                                                                                        gatgatGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTgg                <  3:90761/148‑1 (MQ=255)                                                                                                                                                   aTCGGTCGCCAGCGGCACCGGCGAAGCCAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGCCCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGa    >  5:403130/1‑149 (MQ=255)                                                                                                                                                   aTCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACt      <  8:200623/147‑1 (MQ=255)                                                                                                                                                     cGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACg  >  7:145045/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     ATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACG  >  NC_000913/1729717‑1730013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑