Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,729,865 C→T 5.9% S260L (TCG→TTG)  lhr → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,729,8650CT 5.9% 216.8 / 2.5 68S260L (TCG→TTG) lhrputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base C (26/38);  new base T (2/2);  total (28/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

ATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACG  >  NC_000913/1729717‑1730013
                                                                                                                                                    |                                                                                                                                                    
aTTGGCCTTTCTGCCACTGTGCTCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCAGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGACGTACCGGTCGCAAATAAGGATGATGTCTCATc                                                                                                                                                      >  7:168669/1‑149 (MQ=255)
aTTGGACTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATc                                                                                                                                                      >  3:367726/1‑149 (MQ=255)
    gCCTTTCTGCCTCTGTGCGCTCAGCCAGCGATGTGGCATCATTTCTTTGTGGCCATCGCCCGGGTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCCGTCGCCAATATGGATGATGTCTCATCGGTc                                                                                                                                                  <  4:260138/149‑1 (MQ=255)
    gCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTc                                                                                                                                                  <  1:355048/149‑1 (MQ=255)
    gCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTc                                                                                                                                                  >  6:24843/1‑149 (MQ=255)
    gCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGGGGCAGCTTTTCTTTGTGGCGATCGCCCGGTTAGGGTAGTCTACCCGCCCGCAATGCGCCATCCGCAGATTCGAATTGTCGTACCGGGCGCCAATATGGATGATGTCTCATCGGTc                                                                                                                                                  <  4:254887/149‑1 (MQ=255)
     ccTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCg                                                                                                                                                 <  7:100908/149‑1 (MQ=255)
          cTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAg                                                                                                                                             <  5:244077/148‑1 (MQ=255)
             ccACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGc                                                                                                                                            <  7:422445/146‑1 (MQ=255)
             ccACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCgg                                                                                                                                          >  3:382814/1‑148 (MQ=255)
             ccACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGc                                                                                                                                         >  4:184410/1‑149 (MQ=255)
                   tgCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCgg                                                                                                                                    <  3:201307/148‑1 (MQ=255)
                   tgCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCg                                                                                                                                     <  4:125133/147‑1 (MQ=255)
                      gcTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGaa                                                                                                                                <  3:239326/149‑1 (MQ=255)
                              agcGATGTGGCAGCATTTCTTGGTGGCGATCGCCAGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCa                                                                                                                        <  2:113214/149‑1 (MQ=255)
                               gcGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCAt                                                                                                                       <  4:394141/149‑1 (MQ=255)
                                       gcagcaTTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATTGGTCGCCAGCGGCACCGGCGAAGACAGCCATgccggccg                                                                                                               >  6:18848/1‑149 (MQ=255)
                                       gcagcaTTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATTGGTCGCCAGCGGCACCGGCGAAGACAGCCATgccggccg                                                                                                               >  6:24470/1‑149 (MQ=255)
                                            aTTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGa                                                                                                                              <  7:322043/129‑1 (MQ=255)
                                            aTTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGa                                                                                                                              >  8:322043/1‑129 (MQ=255)
                                             tttCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAgg                                                                                                         <  8:170776/149‑1 (MQ=255)
                                                cTTGGGGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCt                                                                                                       <  4:300559/148‑1 (MQ=255)
                                                   ggtggCGATCGCCCGTGTACGGTAGGCAACCCGCCCGAAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATTATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTc                                                                                                      <  6:407621/146‑1 (MQ=255)
                                                   ggtggCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGTATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTc                                                                                                      <  6:22784/146‑1 (MQ=255)
                                                    gtggCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCACCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCAt                                                                                                   <  2:12945/148‑1 (MQ=255)
                                                        cGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGc                                                                                              <  3:184410/149‑1 (MQ=255)
                                                         gATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGc                                                                                              >  5:172123/1‑148 (MQ=255)
                                                          aTCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCa                                                                                            >  4:90761/1‑149 (MQ=255)
                                                           tCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCa                                                                                            >  4:257480/1‑148 (MQ=255)
                                                             gCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCata                                                                                          >  6:340154/1‑148 (MQ=255)
                                                               ccGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGCTGTCTCATCGGTCGCCAGCGGCACCGGCGCAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCatatat                                                                                       >  3:322869/1‑149 (MQ=255)
                                                               ccGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCatatat                                                                                       <  4:382814/149‑1 (MQ=255)
                                                                cGGTTACGGTAGTCAACGCGCCCGCAATGCGCCATCCGCAGATACGTATTGTCGTACCGGGCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATAtt                                                                                      <  8:168669/149‑1 (MQ=255)
                                                                      cGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAAc                                                                                 <  6:197139/148‑1 (MQ=255)
                                                                      cGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACg                                                                                <  5:277526/149‑1 (MQ=255)
                                                                       ggTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCATTATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTTGCCATATATTGAAACgg                                                                               <  6:107116/149‑1 (MQ=255)
                                                                       ggTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACgg                                                                               <  5:33676/149‑1 (MQ=255)
                                                                       ggTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACgg                                                                               <  2:214232/149‑1 (MQ=255)
                                                                         tAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCCTCTGGCCATATATTGAAACGGGt                                                                             >  2:324538/1‑149 (MQ=255)
                                                                            tCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATc                                                                          >  4:49861/1‑149 (MQ=255)
                                                                               aCCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATTGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCtt                                                                       <  5:24470/149‑1 (MQ=255)
                                                                               aCCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGGCGCCAATATGGATGATGTCTCATTGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCtt                                                                       <  5:18848/149‑1 (MQ=255)
                                                                                 ccgcccgcAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTtga                                                                     >  4:88541/1‑149 (MQ=255)
                                                                                    cccgcAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTtgatg                                                                   >  6:173371/1‑148 (MQ=255)
                                                                                         aaTGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAgtgt                                                             >  2:399461/1‑149 (MQ=255)
                                                                                             cgcCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCAGCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTgc                                                          <  5:24843/148‑1 (MQ=255)
                                                                                                  tCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATc                                                    >  7:207437/1‑149 (MQ=255)
                                                                                                  tCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATc                                                    <  3:120312/149‑1 (MQ=255)
                                                                                                     gCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCt                                                 >  7:200623/1‑149 (MQ=255)
                                                                                                        gATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGa                                              >  8:56787/1‑149 (MQ=255)
                                                                                                         aTACGAATTGTCGTACCGCTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCAAATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGAc                                             >  8:153814/1‑149 (MQ=255)
                                                                                                          tACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGAcc                                            <  6:172123/149‑1 (MQ=255)
                                                                                                             gAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCatat                                                                                         <  8:56101/101‑1 (MQ=255)
                                                                                                             gAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCatat                                                                                         >  7:56101/1‑101 (MQ=255)
                                                                                                             gAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCAtt                                         <  3:91834/149‑1 (MQ=255)
                                                                                                             gAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCAtt                                         <  1:399461/149‑1 (MQ=255)
                                                                                                                   tCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAg                                                                <  1:62582/120‑1 (MQ=255)
                                                                                                                   tCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAg                                                                >  2:62582/1‑120 (MQ=255)
                                                                                                                     gTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCATCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTa                                  <  5:173371/148‑1 (MQ=255)
                                                                                                                     gTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTAc                                 <  2:35092/149‑1 (MQ=255)
                                                                                                                      tACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCtt                                                                       >  2:306827/1‑110 (MQ=255)
                                                                                                                      tACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCtt                                                                       <  1:306827/110‑1 (MQ=255)
                                                                                                                         cGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAAt                             >  3:390790/1‑149 (MQ=255)
                                                                                                                           gTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCAGTGTCTTTACTAATTc                           >  6:135963/1‑149 (MQ=255)
                                                                                                                                cAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCggg                      >  7:382231/1‑149 (MQ=255)
                                                                                                                                      ggATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTcgc                         >  5:386611/1‑140 (MQ=255)
                                                                                                                                      ggATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTcgc                         <  6:386611/140‑1 (MQ=255)
                                                                                                                                       gatgatGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTgg                <  3:90761/148‑1 (MQ=255)
                                                                                                                                                  aTCGGTCGCCAGCGGCACCGGCGAAGCCAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGCCCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGa    >  5:403130/1‑149 (MQ=255)
                                                                                                                                                  aTCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACt      <  8:200623/147‑1 (MQ=255)
                                                                                                                                                    cGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACg  >  7:145045/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACG  >  NC_000913/1729717‑1730013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: