Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,372,984 G→A 5.1% T31T (ACG→ACA arnF → undecaprenyl phosphate‑alpha‑L‑ara4N exporter; flippase ArnEF subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,372,9840GA 5.1% 245.9 / 2.3 78T31T (ACG→ACAarnFundecaprenyl phosphate‑alpha‑L‑ara4N exporter; flippase ArnEF subunit
Reads supporting (aligned to +/- strand):  ref base G (39/34);  new base A (2/2);  total (42/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.82e-01

GTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCA  >  NC_000913/2372850‑2373132
                                                                                                                                      |                                                                                                                                                    
gTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGAttt                                                                                                                                        >  4:229256/1‑149 (MQ=255)
    attattGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATc                                                                                                                                    >  2:140586/1‑149 (MQ=255)
    attattGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGATGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATc                                                                                                                                    >  1:327287/1‑149 (MQ=255)
     ttattGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATcg                                                                                                                                   >  1:192498/1‑149 (MQ=255)
       attGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATcgc                                                                                                                                  <  1:324231/148‑1 (MQ=255)
        ttGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCgg                                                                                                                                >  8:6849/1‑149 (MQ=255)
          ggcggcATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCAc                                                                                                                                                   <  2:82733/128‑1 (MQ=255)
          ggcggcATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCAc                                                                                                                                                   >  1:82733/1‑128 (MQ=255)
           gcggcATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGctc                                                                                                                             <  3:382676/149‑1 (MQ=255)
              gcATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGTTTATTCAGCGTCATTATTGCCTCATTTGCGCAATTAAGACTGTGTTTTGCGGCGAGGCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGt                                                                                                                          <  7:357188/149‑1 (MQ=255)
               cATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGtt                                                                                                                         <  5:326482/149‑1 (MQ=255)
                      aTCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCtttt                                                                                                                  >  8:330081/1‑149 (MQ=255)
                        ccTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTgg                                                                                                                >  5:116311/1‑149 (MQ=255)
                         cTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTgg                                                                                                                <  1:195821/148‑1 (MQ=255)
                         cTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTgg                                                                                                                <  3:391004/148‑1 (MQ=255)
                           cGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCt                                                                                                             >  8:338971/1‑149 (MQ=255)
                            gggAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTg                                                                                                            >  2:26727/1‑149 (MQ=255)
                             ggAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATATGCCGACGATGACTCACATATGGGATTTTATAGCGGATCTGTTAGCTTTTGGCCTg                                                                                                            >  3:292522/1‑148 (MQ=255)
                                   cGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGccc                                                                                                     <  6:213729/149‑1 (MQ=255)
                                   cGGTGTAATGGGCCTGATGGGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGccc                                                                                                     <  5:301290/149‑1 (MQ=255)
                                        tAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATa                                                                                                <  8:68848/149‑1 (MQ=255)
                                          aTGGGGCTGTTGTGGGGCTTATTCAGCGTCAATATTGGCTCAGTTGCGCAATTACGCCTGGGTGTTGCGGCGTGTCATCTGCCGCCGATGACGCAATTATGGGATTTTATCGCGGCTCTGTTAGCTTTTTGCCTGGATGCCCGGATACt                                                                                              <  5:157058/149‑1 (MQ=255)
                                           tGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTg                                                                                             >  8:354503/1‑149 (MQ=255)
                                            gggCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGt                                                                                            >  1:130012/1‑149 (MQ=255)
                                                   aTGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCg                                                                                      <  6:116311/148‑1 (MQ=255)
                                                     gtgGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGgc                                                                                   >  4:214095/1‑149 (MQ=255)
                                                          gATTATTCAGCGTCATTATTGCCTCAGTGGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGctgctg                                                                              <  6:210411/149‑1 (MQ=255)
                                                            ttattCAGCGTCATTTCTGCCTCAGTTGCGCAATTAAGCCTGGGTGTTGCTGCGAGTCATCTGCCGCCGATGACGCACTAATGTGATTTTATCCCGGCTCTGTTAGGGTTTGGCCTTGATGCCCGGATTCTGTTACTCGGGCTTCTggg                                                                            <  6:309484/149‑1 (MQ=255)
                                                               ttCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATCGGATTTTATCGCGGCTCTCTTAGCTATTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCAGGGTTa                                                                         >  2:395584/1‑149 (MQ=255)
                                                                  aGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCt                                                                                                             >  7:424088/1‑110 (MQ=255)
                                                                  aGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCt                                                                                                             <  8:424088/110‑1 (MQ=255)
                                                                  aGCGTCATTATTGCCTCAGGTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATc                                                                       <  1:258608/148‑1 (MQ=255)
                                                                   gCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTg                                                                     <  7:6849/149‑1 (MQ=255)
                                                                      tCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCtt                                                                  >  5:392336/1‑149 (MQ=255)
                                                                          tattGCCACAGTAGCGCAATTAAGCCTGGTTTTTGCGGAGAGTCATCTGACGCCGATGAAGCACTTAAGGCATTATATCGCGGATATGTTAGCTTTTGCACTGGAAGCCCGGATACTGTTACTCGGGCAGCAGGGTTATCTGCTTTACg                                                              >  8:351211/1‑149 (MQ=255)
                                                                           attGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGt                                                             >  8:302483/1‑149 (MQ=255)
                                                                              gCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGttt                                                           <  2:130012/148‑1 (MQ=255)
                                                                               ccTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGttttt                                                         >  5:45291/1‑149 (MQ=255)
                                                                               ccTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGttttt                                                         >  4:64712/1‑149 (MQ=255)
                                                                               ccTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGttttt                                                         >  6:365010/1‑149 (MQ=255)
                                                                               ccTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGttttt                                                         >  7:80936/1‑149 (MQ=255)
                                                                               ccTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCGCTGTTAGCTTTTGGCCTGGATGACCGGATAATGTTACGCGGGCTGCTGGGTTATCTGCTTTCCGttttt                                                         >  8:84503/1‑149 (MQ=255)
                                                                                cTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGtttttt                                                        >  8:80288/1‑149 (MQ=255)
                                                                                cTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGtttttt                                                        >  7:195631/1‑149 (MQ=255)
                                                                                cTCAGTTGCGCAATTAAGCCTGGGGTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGtttttt                                                        <  8:195631/149‑1 (MQ=255)
                                                                                   aGTTGCGCAATTACGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCAGTTTTGTGTTTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCGGGATACTGTTAGTCTGGCTGCTGGGTTATCTGCTTTCCGTTTTttgtt                                                     <  2:109044/149‑1 (MQ=255)
                                                                                   aGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTttgtt                                                     <  5:365010/149‑1 (MQ=255)
                                                                                    gTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTttgttg                                                    <  1:140586/149‑1 (MQ=255)
                                                                                    gTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTttgttg                                                    <  3:229256/149‑1 (MQ=255)
                                                                                           aaTTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATaa                                              >  2:276177/1‑148 (MQ=255)
                                                                                           aaTTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATaa                                              >  7:382293/1‑148 (MQ=255)
                                                                                            aTTAAGCCTGGGTTTTGCGGCGAGACATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATaaaa                                            >  1:131020/1‑149 (MQ=255)
                                                                                             ttAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAAc                                           <  4:303827/149‑1 (MQ=255)
                                                                                               aaGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGc                                         >  2:377458/1‑149 (MQ=255)
                                                                                                aGCCTGGGTGTCGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGc                                         <  3:242201/148‑1 (MQ=255)
                                                                                                 gCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCtt                                       >  1:222535/1‑149 (MQ=255)
                                                                                                  ccTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCtt                                       >  7:58916/1‑148 (MQ=255)
                                                                                                   cTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCa                                     <  7:302483/149‑1 (MQ=255)
                                                                                                   cTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCa                                     >  2:265514/1‑149 (MQ=255)
                                                                                                    tGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGtttttt                                                        <  3:95891/129‑1 (MQ=255)
                                                                                                    tGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGtttttt                                                        >  4:95891/1‑129 (MQ=255)
                                                                                                    tGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCa                                     >  6:256930/1‑148 (MQ=255)
                                                                                                    tGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCa                                     >  8:211045/1‑148 (MQ=255)
                                                                                                          tggcggcgAGTCATCTGCCGCCGATGCAGCACTTATGGTCTTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACtt                              <  4:292522/147‑1 (MQ=255)
                                                                                                             cggcgAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTgc                            <  7:211045/148‑1 (MQ=255)
                                                                                                              ggagAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTgcg                           <  2:91742/145‑1 (MQ=255)
                                                                                                               gcgAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTGTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCTGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTTGTATAAAACGCTTCATAAACTTGCGCt                         <  7:351211/149‑1 (MQ=255)
                                                                                                                 gAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTaa                       <  8:80936/149‑1 (MQ=255)
                                                                                                                      aTCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCa                    >  8:78960/1‑147 (MQ=255)
                                                                                                                      aTCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCa                    >  5:393721/1‑147 (MQ=255)
                                                                                                                        cTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGc                >  7:335514/1‑149 (MQ=255)
                                                                                                                         ttccgccgATGAGGCACTTATGGGATTTTATCGCGGCTCGGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGGTGGGTTATCTGCTTTCCTTTTTTTGTTGGTATAATACGCTTCATAAACTTGCGCTAAGCAAAGc                <  2:327287/146‑1 (MQ=255)
                                                                                                                         tGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGcc               >  8:241942/1‑149 (MQ=255)
                                                                                                                           ccgccgATGACACACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTa             >  8:179027/1‑149 (MQ=255)
                                                                                                                           ccgccgATGACACACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTa             >  6:253839/1‑149 (MQ=255)
                                                                                                                           ccgccgATGACACACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTa             <  7:179027/149‑1 (MQ=255)
                                                                                                                           ccgccgATGACACACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTa             <  5:253839/149‑1 (MQ=255)
                                                                                                                                 aTGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCtt       <  7:338971/149‑1 (MQ=255)
                                                                                                                                      gCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTAt                                                                        <  6:307368/79‑1 (MQ=255)
                                                                                                                                      gCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTAt                                                                        >  5:307368/1‑79 (MQ=255)
                                                                                                                                      gCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCa  <  8:335514/149‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                                    
GTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCATCTGCCGCCGATGACGCACTTATGGGATTTTATCGCGGCTCTGTTAGCTTTTGGCCTGGATGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCA  >  NC_000913/2372850‑2373132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: