Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,767,667 T→G 5.7% intergenic (+312/‑43) rnlB → / → yfjP CP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; 50S ribosome‑binding GTPase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,767,6670TG 5.7% 379.9 / 8.8 120intergenic (+312/‑43)rnlB/yfjPCP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
Reads supporting (aligned to +/- strand):  ref base T (61/52);  new base G (4/3);  total (65/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 124 total aligned reads displayed.

TCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTAC  >  NC_000913/2767518‑2767814
                                                                                                                                                     |                                                                                                                                                   
tcAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCCCTGAACAATCCGATGAACGGCAGAACCt                                                                                                                                                     >  4:317263/1‑149 (MQ=255)
  aataatGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTc                                                                                                                                                    >  1:16066/1‑149 (MQ=255)
  aataatGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTc                                                                                                                                                    >  7:341632/1‑149 (MQ=255)
   ataatGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTcg                                                                                                                                                   >  1:305824/1‑149 (MQ=255)
     aatGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTcgcg                                                                                                                                                 >  6:359447/1‑149 (MQ=255)
            ccAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGc                                                                                                                                          >  7:412279/1‑149 (MQ=255)
             cAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGcc                                                                                                                                         >  1:366727/1‑149 (MQ=255)
                 tttCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCGCGAGGTTCTGCCGtt                                                                                                                                      >  4:359062/1‑148 (MQ=255)
                    cTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGttttt                                                                                                                                   <  3:306520/148‑1 (MQ=255)
                     tGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGt                                                                                                                                 >  1:35464/1‑149 (MQ=255)
                      ggAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGtt                                                                                                                                >  5:252981/1‑149 (MQ=255)
                       gAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGt                                                                                                                                 <  6:105277/147‑1 (MQ=255)
                        aaaTCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGtttt                                                                                                                              <  6:199492/149‑1 (MQ=255)
                           tCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATATATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACt                                                                                                                           <  5:227977/149‑1 (MQ=255)
                              tATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACtttt                                                                                                                        >  7:290554/1‑149 (MQ=255)
                              tATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCGCGAGGTTCTGc                                                                                                                                          <  6:230463/131‑1 (MQ=255)
                                tgatgCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACtttt                                                                                                                        <  8:290554/147‑1 (MQ=255)
                                tgatgCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTc                                                                                                                       <  8:341632/148‑1 (MQ=255)
                                tgatgCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTc                                                                                                                       <  8:2693/148‑1 (MQ=255)
                                tgatgCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCa                                                                                                                      <  7:207524/149‑1 (MQ=255)
                                 gatgCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACtttt                                                                                                                        >  5:323896/1‑146 (MQ=255)
                                   tgCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATTTCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCTTTTTTGTTTTACTTTTAagag                                                                                                                   >  5:21578/1‑149 (MQ=255)
                                    gCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACATTTCagag                                                                                                                   >  6:206724/1‑148 (MQ=255)
                                      cTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTa                                                                                                                             >  7:354701/1‑136 (MQ=255)
                                      cTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGAGGTTCTGCCGTTTTTGTTTTACTTTTCAgaggag                                                                                                                >  5:218098/1‑149 (MQ=255)
                                       tttCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAgt                                                                                                               >  7:323831/1‑149 (MQ=255)
                                         tCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAgtg                                                                                                              <  7:169788/148‑1 (MQ=255)
                                          cccGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGt                                                                                                                                 >  8:112828/1‑128 (MQ=255)
                                          cccGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGt                                                                                                                                 <  7:112828/128‑1 (MQ=255)
                                            cGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGACCACTCCGATGCACGGCAGAACCTCGCGGGTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGtga                                                                                                          <  2:292721/149‑1 (MQ=255)
                                              gTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCATAACCTCGCGGTTCTTCCTTTTTTGTTTTACTTTTCAGAGGAGTGGtgat                                                                                                         >  3:91362/1‑148 (MQ=255)
                                              gTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTg                                                                                                                                  >  5:377705/1‑123 (MQ=255)
                                              gTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTg                                                                                                                                  <  6:377705/123‑1 (MQ=255)
                                                      tGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAAtttt                                                                                               <  3:317263/149‑1 (MQ=255)
                                                        cTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAAtttt                                                                                               <  3:291414/148‑1 (MQ=255)
                                                        cTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAAtttt                                                                                               <  6:323896/148‑1 (MQ=255)
                                                        cTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTg                                                                                              <  2:242584/149‑1 (MQ=255)
                                                           tgATTTCAGATAGTTTCTGCATTCTTTGTGCTTTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGaa                                                                                            <  6:252981/148‑1 (MQ=255)
                                                              gTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTc                                                                                        <  1:337156/149‑1 (MQ=255)
                                                               ttCAGAAAGATTCTGCAATCTATGTGAATTGAAAAAATCTTCAGATAGATATCATTCTCCTCAACAATCCGATGAACGGCAGAACCTCGCGCTGGTGCCCTTTATGATTTACTTTTCAGAGGAGTGGTCATGAAAAATTTTGAAGTTCt                                                                                       >  6:37197/1‑149 (MQ=255)
                                                                tCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATACGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTAATTTTCAGATGATTGGTGATTAAAAATTATGAAGTTCTg                                                                                      >  6:114981/1‑149 (MQ=255)
                                                                 cAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTg                                                                                      <  8:195254/148‑1 (MQ=255)
                                                                 cAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTg                                                                                      <  7:307215/148‑1 (MQ=255)
                                                                 cAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTg                                                                                      >  7:425948/1‑148 (MQ=255)
                                                                    aaaGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCCGAACATCGCGTTTCTGCCGTTTTTGTTTTACTTTTCTGAGGAGTGGTGATGAAAAATTTTGCAGTTCtgctgc                                                                                  >  8:401955/1‑149 (MQ=255)
                                                                    aaaGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAgagga                                                                                                                 >  7:127580/1‑118 (MQ=255)
                                                                    aaaGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAgagga                                                                                                                 <  8:127580/118‑1 (MQ=255)
                                                                    aaaGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGc                                                                                  >  2:302010/1‑149 (MQ=255)
                                                                         ttCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTa                                                                             >  4:321263/1‑149 (MQ=255)
                                                                         ttCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGACAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTa                                                                             <  4:144099/149‑1 (MQ=255)
                                                                            tGCAATCTATGTGCATTGAAAAAATAATCAGATAGAAATCATTCTCCAGATCAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTATTACTGTTAAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTa                                                                             >  5:361690/1‑146 (MQ=255)
                                                                             gCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGCACAATCCGATGAACGGCAGAGCCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGGGATGAAAAATTTTGAAGTTCTGCAGCCACTACAg                                                                          >  6:250166/1‑148 (MQ=255)
                                                                             gCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAg                                                                          >  7:71502/1‑148 (MQ=255)
                                                                                    aTGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCACCCACTACAGAACTCCCt                                                                  >  7:345027/1‑149 (MQ=255)
                                                                                       tgCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCttt                                                                <  4:280449/148‑1 (MQ=255)
                                                                                       tgCATTGAAAAAATCTTCAGATAGATATCACTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTc                                                               >  5:245297/1‑149 (MQ=255)
                                                                                         cATTTAAAAAATCTTCAGATAGATTTCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCTCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTTAAGTTCTGCAGCCACTACAGAACTCCCTTTcc                                                              <  5:37197/148‑1 (MQ=255)
                                                                                          aTTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCgg                                                            >  1:132449/1‑149 (MQ=255)
                                                                                          aTTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCgg                                                            >  1:4129/1‑149 (MQ=255)
                                                                                           ttGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGATTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCgg                                                            >  5:273747/1‑148 (MQ=255)
                                                                                           ttGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGt                                                           >  5:65587/1‑149 (MQ=255)
                                                                                           ttGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGt                                                           <  7:255469/149‑1 (MQ=255)
                                                                                            tGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTg                                                                                      >  3:265734/1‑121 (MQ=255)
                                                                                            tGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTg                                                                                      <  4:265734/121‑1 (MQ=255)
                                                                                             gAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTc                                                          >  1:214810/1‑148 (MQ=255)
                                                                                              aaaaaaTCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTa                                                        >  1:154114/1‑149 (MQ=255)
                                                                                               aaaaaTCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGaa                                                                                            >  2:218863/1‑112 (MQ=255)
                                                                                                aaaaTCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTAcc                                                      >  4:175195/1‑149 (MQ=255)
                                                                                                        cAGCTAGATATCATTCTCCTGAACTATCCGATGAACGGCAGAACCTCGCGGTTCTGGCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATgg                                               <  1:175301/148‑1 (MQ=255)
                                                                                                        cAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATgg                                               <  2:35464/148‑1 (MQ=255)
                                                                                                        cAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATgg                                               <  2:366727/148‑1 (MQ=255)
                                                                                                        cAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATg                                                <  5:211945/147‑1 (MQ=255)
                                                                                                        cAGATAGATATCATTCTAATGAACAATCCGATGAAAGGCAGAACCTCGAGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTTGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATgg                                               <  8:294534/148‑1 (MQ=255)
                                                                                                           atagataTCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTAt                                           >  5:113266/1‑149 (MQ=255)
                                                                                                                tatCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGaa                                      >  4:359849/1‑149 (MQ=255)
                                                                                                                tatCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGaa                                      <  2:305824/149‑1 (MQ=255)
                                                                                                                  tCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAAc                                     <  1:302010/148‑1 (MQ=255)
                                                                                                                  tCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAAc                                     <  2:4129/148‑1 (MQ=255)
                                                                                                                   cATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTGCAGCCGGTTTTGGTTTACTTTTCAGAGGAGTGGTGATGCAAAAATTTGAAGTTCTGCAGCCACGACAGATCTCCCTATCCGGTCTACGGGTATGGGTAACTgctcg                                    >  4:214277/1‑144 (MQ=255)
                                                                                                                        tcCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATAtt                              >  6:87906/1‑149 (MQ=255)
                                                                                                                          cttaaCTATCCGATGAACGTCAGAACCTCGCTGTTCTGGCGTTTTTGTTTTTCTTTTCAGAGGAGTGGTGTTGAAAAATTTTGAATTTGTCCAGCTACTACAGAACTCCCTTTGCGGTCTACCGCTATGGGTATCTGAACGTATATTg                             <  5:114981/145‑1 (MQ=255)
                                                                                                                          cTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgc                            <  8:425948/149‑1 (MQ=255)
                                                                                                                          cTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTg                             >  1:224450/1‑148 (MQ=255)
                                                                                                                             aaCAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGACCTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgcag                          >  2:45932/1‑148 (MQ=255)
                                                                                                                             aaCAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgcagc                         <  7:246212/149‑1 (MQ=255)
                                                                                                                             aaCAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGGGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgcagc                         <  8:412279/149‑1 (MQ=255)
                                                                                                                                aaTCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGa                      <  2:132449/149‑1 (MQ=255)
                                                                                                                                    cGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGTTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGTTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATaaa                  <  5:206724/149‑1 (MQ=255)
                                                                                                                                    cGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATaaa                  >  2:318436/1‑149 (MQ=255)
                                                                                                                                      aaaaaCGGCAGAACCGCGAGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTAcc                                                      <  5:35895/108‑1 (MQ=255)
                                                                                                                                      aaaaaCGGCAGAACCGCGAGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAAt                 >  7:57707/4‑148 (MQ=255)
                                                                                                                                       tGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCa               <  2:214810/149‑1 (MQ=255)
                                                                                                                                            ggCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCCCTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTaa          >  3:189285/1‑149 (MQ=255)
                                                                                                                                            ggCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTaa          >  2:326272/1‑149 (MQ=255)
                                                                                                                                              cAGAACCTCGTGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAAcc        <  4:244495/149‑1 (MQ=255)
                                                                                                                                              cAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAAcc        <  8:71502/149‑1 (MQ=255)
                                                                                                                                               aGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAAcc        >  7:93528/1‑148 (MQ=255)
                                                                                                                                                     tCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAATTTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTAc  >  6:365167/1‑148 (MQ=255)
                                                                                                                                                     tCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAAt                 <  4:92195/133‑1 (MQ=255)
                                                                                                                                                     tCGCGGTTCTGCCGTTTTTGGTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAAt                 >  3:92195/1‑133 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTAC  >  NC_000913/2767518‑2767814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: