Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,767,670 C→A 7.4% intergenic (+315/‑40) rnlB → / → yfjP CP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; 50S ribosome‑binding GTPase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,767,6700CA 7.4% 337.6 / 12.4 118intergenic (+315/‑40)rnlB/yfjPCP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
Reads supporting (aligned to +/- strand):  ref base C (57/51);  new base A (5/4);  total (62/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

Only 100 of 122 total aligned reads displayed.

AATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGC  >  NC_000913/2767523‑2767818
                                                                                                                                                   |                                                                                                                                                    
aatGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTcgcg                                                                                                                                                     >  6:359447/1‑149 (MQ=255)
    tCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGtt                                                                                                                                                  >  2:175301/1‑148 (MQ=255)
      aCCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTg                                                                                                                                               >  4:306520/1‑149 (MQ=255)
       ccAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGc                                                                                                                                              >  7:412279/1‑149 (MQ=255)
        cAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGcc                                                                                                                                             >  1:366727/1‑149 (MQ=255)
            tttCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCGCGAGGTTCTGCCGtt                                                                                                                                          >  4:359062/1‑148 (MQ=255)
            tttCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCGCGAGGTTCTGCCGtt                                                                                                                                          <  3:359062/148‑1 (MQ=255)
               cTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGttttt                                                                                                                                       <  3:306520/148‑1 (MQ=255)
                tGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGt                                                                                                                                     >  1:35464/1‑149 (MQ=255)
                 ggAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCTTTTTTGtt                                                                                                                                    >  7:171851/1‑149 (MQ=255)
                 ggAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGtt                                                                                                                                    >  5:252981/1‑149 (MQ=255)
                   aaaTCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGtttt                                                                                                                                  <  6:199492/149‑1 (MQ=255)
                      tCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATATATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACt                                                                                                                               <  5:227977/149‑1 (MQ=255)
                         tATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCGCGAGGTTCTGc                                                                                                                                              <  6:230463/131‑1 (MQ=255)
                           tgatgCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACtttt                                                                                                                            <  8:290554/147‑1 (MQ=255)
                           tgatgCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTc                                                                                                                           <  8:2693/148‑1 (MQ=255)
                           tgatgCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTc                                                                                                                           <  8:341632/148‑1 (MQ=255)
                           tgatgCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCa                                                                                                                          <  7:207524/149‑1 (MQ=255)
                            gatgCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACtttt                                                                                                                            >  5:323896/1‑146 (MQ=255)
                              tgCCTTTTCCGTTAAATGGTGCTGGGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGATAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCagag                                                                                                                       <  1:354397/149‑1 (MQ=255)
                              tgCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATTTCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCTTTTTTGTTTTACTTTTAagag                                                                                                                       >  5:21578/1‑149 (MQ=255)
                               gCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACATTTCagag                                                                                                                       >  6:206724/1‑148 (MQ=255)
                                 cTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTa                                                                                                                                 <  8:354701/136‑1 (MQ=255)
                                 cTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGAGGTTCTGCCGTTTTTGTTTTACTTTTCAgaggag                                                                                                                    >  5:218098/1‑149 (MQ=255)
                                  tttCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAgt                                                                                                                   >  7:323831/1‑149 (MQ=255)
                                    tCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAgtg                                                                                                                  <  7:169788/148‑1 (MQ=255)
                                     cccGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGt                                                                                                                                     >  8:112828/1‑128 (MQ=255)
                                     cccGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGt                                                                                                                                     <  7:112828/128‑1 (MQ=255)
                                       cGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGACCACTCCGATGCACGGCAGAACCTCGCGGGTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGtga                                                                                                              <  2:292721/149‑1 (MQ=255)
                                        ggTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGACAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGtgat                                                                                                             >  3:144099/1‑149 (MQ=255)
                                         gTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCATAACCTCGCGGTTCTTCCTTTTTTGTTTTACTTTTCAGAGGAGTGGtgat                                                                                                             >  3:91362/1‑148 (MQ=255)
                                         gTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTg                                                                                                                                      <  6:377705/123‑1 (MQ=255)
                                         gTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTg                                                                                                                                      >  5:377705/1‑123 (MQ=255)
                                                   cTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAAtttt                                                                                                   <  6:323896/148‑1 (MQ=255)
                                                   cTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAAtttt                                                                                                   <  3:291414/148‑1 (MQ=255)
                                                   cTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTg                                                                                                  <  2:300243/149‑1 (MQ=255)
                                                      tgATTTCAGATAGTTTCTGCATTCTTTGTGCTTTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGaa                                                                                                <  6:252981/148‑1 (MQ=255)
                                                         gTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTc                                                                                            <  1:337156/149‑1 (MQ=255)
                                                         gTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTc                                                                                            >  2:337156/1‑149 (MQ=255)
                                                           tCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATACGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTAATTTTCAGATGATTGGTGATTAAAAATTATGAAGTTCTg                                                                                          >  6:114981/1‑149 (MQ=255)
                                                            cAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTg                                                                                          <  7:307215/148‑1 (MQ=255)
                                                            cAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTg                                                                                          <  8:195254/148‑1 (MQ=255)
                                                            cAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTg                                                                                          >  7:425948/1‑148 (MQ=255)
                                                               aaaGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCCGAACATCGCGTTTCTGCCGTTTTTGTTTTACTTTTCTGAGGAGTGGTGATGAAAAATTTTGCAGTTCtgctgc                                                                                      >  8:401955/1‑149 (MQ=255)
                                                               aaaGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAgagga                                                                                                                     <  8:127580/118‑1 (MQ=255)
                                                               aaaGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGc                                                                                      >  2:302010/1‑149 (MQ=255)
                                                                    ttCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTa                                                                                 >  4:321263/1‑149 (MQ=255)
                                                                    ttCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGACAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTa                                                                                 <  4:144099/149‑1 (MQ=255)
                                                                       tGCAATCTATGTGCATTGAAAAAATAATCAGATAGAAATCATTCTCCAGATCAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTATTACTGTTAAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTa                                                                                 >  5:361690/1‑146 (MQ=255)
                                                                        gCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAg                                                                              >  7:71502/1‑148 (MQ=255)
                                                                         cAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGaa                                                                            >  4:209705/1‑149 (MQ=255)
                                                                               aTGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCACCCACTACAGAACTCCCt                                                                      >  7:345027/1‑149 (MQ=255)
                                                                                  tgCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCttt                                                                    <  4:280449/148‑1 (MQ=255)
                                                                                  tgCATTGAAAAAATCTTCAGATAGATATCACTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTc                                                                   >  5:245297/1‑149 (MQ=255)
                                                                                    cATTTAAAAAATCTTCAGATAGATTTCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCTCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTTAAGTTCTGCAGCCACTACAGAACTCCCTTTcc                                                                  <  5:37197/148‑1 (MQ=255)
                                                                                     aTTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCgg                                                                >  1:132449/1‑149 (MQ=255)
                                                                                      ttGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGATTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCgg                                                                >  5:273747/1‑148 (MQ=255)
                                                                                      ttGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGt                                                               >  5:65587/1‑149 (MQ=255)
                                                                                      ttGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGt                                                               <  7:255469/149‑1 (MQ=255)
                                                                                       tGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTg                                                                                          >  3:265734/1‑121 (MQ=255)
                                                                                        gAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTc                                                              >  1:214810/1‑148 (MQ=255)
                                                                                         aaaaaaTCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTa                                                            >  1:154114/1‑149 (MQ=255)
                                                                                          aaaaaTCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGaa                                                                                                >  2:218863/1‑112 (MQ=255)
                                                                                          aaaaaTCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGaa                                                                                                <  1:218863/112‑1 (MQ=255)
                                                                                           aaaaTCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTAcc                                                          >  4:175195/1‑149 (MQ=255)
                                                                                                   cAGCTAGATATCATTCTCCTGAACTATCCGATGAACGGCAGAACCTCGCGGTTCTGGCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATgg                                                   <  1:175301/148‑1 (MQ=255)
                                                                                                   cAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATgg                                                   <  2:35464/148‑1 (MQ=255)
                                                                                                   cAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATgg                                                   <  2:366727/148‑1 (MQ=255)
                                                                                                   cAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATg                                                    <  5:211945/147‑1 (MQ=255)
                                                                                                   cAGATAGATATCATTCTAATGAACAATCCGATGAAAGGCAGAACCTCGAGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTTGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATgg                                                   <  8:294534/148‑1 (MQ=255)
                                                                                                   aagatagatATCATTCTCATGAACAATCAGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTAt                                                     <  4:91362/145‑1 (MQ=255)
                                                                                                      atagataTCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTAt                                               >  5:113266/1‑149 (MQ=255)
                                                                                                           tatCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGaa                                          <  2:305824/149‑1 (MQ=255)
                                                                                                             tCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAAc                                         <  2:4129/148‑1 (MQ=255)
                                                                                                             tCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAAc                                         <  1:302010/148‑1 (MQ=255)
                                                                                                              cATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTGCAGCCGGTTTTGGTTTACTTTTCAGAGGAGTGGTGATGCAAAAATTTGAAGTTCTGCAGCCACGACAGATCTCCCTATCCGGTCTACGGGTATGGGTAACTgctcg                                        >  4:214277/1‑144 (MQ=255)
                                                                                                                   tcCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATAtt                                  >  6:87906/1‑149 (MQ=255)
                                                                                                                     cttaaCTATCCGATGAACGTCAGAACCTCGCTGTTCTGGCGTTTTTGTTTTTCTTTTCAGAGGAGTGGTGTTGAAAAATTTTGAATTTGTCCAGCTACTACAGAACTCCCTTTGCGGTCTACCGCTATGGGTATCTGAACGTATATTg                                 <  5:114981/145‑1 (MQ=255)
                                                                                                                     cTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCCCTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTg                                 >  5:80177/1‑148 (MQ=255)
                                                                                                                     cTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgc                                <  8:425948/149‑1 (MQ=255)
                                                                                                                        aaCAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgcagc                             <  7:246212/149‑1 (MQ=255)
                                                                                                                        aaCAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgca                               <  6:415305/147‑1 (MQ=255)
                                                                                                                        aaCAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGGGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgcagc                             <  8:412279/149‑1 (MQ=255)
                                                                                                                           aaTCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTgcag                              >  7:314893/1‑145 (MQ=255)
                                                                                                                           aaTCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGa                          <  2:132449/149‑1 (MQ=255)
                                                                                                                               cGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGTTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGTTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATaaa                      <  5:206724/149‑1 (MQ=255)
                                                                                                                               cGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATaaa                      >  2:318436/1‑149 (MQ=255)
                                                                                                                                 aaaaaCGGCAGAACCGCGAGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTAcc                                                          <  5:35895/108‑1 (MQ=255)
                                                                                                                                 aaaaaCGGCAGAACCGCGAGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTAcc                                                          >  6:35895/4‑111 (MQ=255)
                                                                                                                                 aaaaaCGGCAGAACCGCGAGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAAt                     >  7:57707/4‑148 (MQ=255)
                                                                                                                                  tGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCa                   <  2:214810/149‑1 (MQ=255)
                                                                                                                                       ggCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCCCTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTaa              >  3:189285/1‑149 (MQ=255)
                                                                                                                                       ggCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTaa              >  7:383980/1‑149 (MQ=255)
                                                                                                                                         cAGAACCTCGTGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAAcc            <  4:244495/149‑1 (MQ=255)
                                                                                                                                         cAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAAcc            <  8:71502/149‑1 (MQ=255)
                                                                                                                                          aGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAAcc            >  7:93528/1‑148 (MQ=255)
                                                                                                                                                tCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAATTTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTAc      >  6:365167/1‑148 (MQ=255)
                                                                                                                                                tCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAAt                     <  4:92195/133‑1 (MQ=255)
                                                                                                                                                tCGCGGTTCTGCCGTTTTTGGTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAAt                     >  3:92195/1‑133 (MQ=255)
                                                                                                                                                   cgGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGc  <  3:359849/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCATTCTCCTGAACAATCCGATGAACGGCAGAACCTCGCGGTTCTGCCGTTTTTGTTTTACTTTTCAGAGGAGTGGTGATGAAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGC  >  NC_000913/2767523‑2767818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: