Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,802,411 C→A 5.0% A355E (GCA→GAA)  nrdE → ribonucleoside‑diphosphate reductase 2, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,802,4110CA 5.0% 315.7 / 4.5 99A355E (GCA→GAA) nrdEribonucleoside‑diphosphate reductase 2, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (44/50);  new base A (2/3);  total (46/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.45e-01

Only 100 of 101 total aligned reads displayed.

ATGGCGCTGTTTTCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTC  >  NC_000913/2802263‑2802559
                                                                                                                                                    |                                                                                                                                                    
aTGGCGCTGTTTTCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCTTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGc                                                                                                                                                      <  5:88259/149‑1 (MQ=255)
     gcTGTTTTCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGaaa                                                                                                                                                 >  8:247690/1‑149 (MQ=255)
         ttttCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATc                                                                                                                                               >  4:152773/1‑147 (MQ=255)
         ttttCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCa                                                                                                                                             <  7:230641/149‑1 (MQ=255)
           ttCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGt                                                                                                                                           >  4:308588/1‑149 (MQ=255)
            tCGCCTTATGACGTAGAGCGAGTTTATGGCACGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTGCCAGTCGCTGGAAGAAATcacgg                                                                                                                                           <  5:413015/148‑5 (MQ=255)
              gCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGttt                                                                                                                                         <  8:274011/148‑1 (MQ=255)
              gCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGttt                                                                                                                                         <  5:52076/148‑1 (MQ=255)
               ccTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGa                                                                                                                                       <  3:308588/149‑1 (MQ=255)
                   aTGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCa                                                                                                                                                     <  1:422293/131‑1 (MQ=255)
                   aTGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCa                                                                                                                                                     >  2:422293/1‑131 (MQ=255)
                       cGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGc                                                                                                                                >  7:260491/1‑148 (MQ=255)
                        gTAGAGCGAGTTTATGGCAAGCCTTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGc                                                                                                                                <  6:65159/147‑1 (MQ=255)
                        gTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAGCGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTa                                                                                                                              >  6:274569/1‑149 (MQ=255)
                        gTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGc                                                                                                                                >  5:65159/1‑147 (MQ=255)
                        gTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTa                                                                                                                              >  5:294010/1‑149 (MQ=255)
                           gagCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATcc                                                                                                                           <  8:380979/149‑1 (MQ=255)
                           gagCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATcc                                                                                                                           <  3:186973/149‑1 (MQ=255)
                           gagCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATc                                                                                                                            <  3:244372/148‑1 (MQ=255)
                              cGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTa                                                                                                                        <  3:152773/149‑1 (MQ=255)
                                  tttATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTAcat                                                                                                                     >  4:220612/1‑148 (MQ=255)
                                     aTGGCAAGCCGTTTGCCGATGTGGCCTTCAGCCAACACTATGACGAACTGGTTGCCGATGTACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTATTCCAGCGACTTGCAGAAATCCATTTTGAGTCCGGCTATCCCTAcatcat                                                                                                                  <  4:313615/148‑1 (MQ=255)
                                     aTGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATg                                                                                                                 >  7:111727/1‑149 (MQ=255)
                                     aTGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATg                                                                                                                 >  3:186694/1‑149 (MQ=255)
                                      tGGCAAGCCGTTTGCCGATTTGGCCATCAGCCAACACTATGACGAACTCGTTCCCCATGAACGCATTCGCAAAAAATACCACAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGTCTATCCCTACATCATg                                                                                                                 >  1:311762/1‑148 (MQ=255)
                                       ggCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTa                                                                                                               >  1:163563/1‑149 (MQ=255)
                                        gCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACTCATTCGCAAAAAATACCTCAACGCCCGTCATTTCTTCCAGAGACTGTCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTa                                                                                                               <  5:26494/148‑1 (MQ=255)
                                        gCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTa                                                                                                               <  7:404240/148‑1 (MQ=255)
                                        gCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTAt                                                                                                              <  4:186694/149‑1 (MQ=255)
                                        gCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTAt                                                                                                              >  1:193331/1‑149 (MQ=255)
                                        gCAAGCCGTGTGCCGATGTGGCCATCAGCCTACACTATGACGAAGTGGTTGCCGATGAACGCATTCGCAAAAACTCCCTCAACGCCCGTGATTTCTTCCAGCGACTGCCAGAATTCCAGTTTGAGTCCGGCTATCCCTACATCATGTAt                                                                                                              <  2:58390/149‑1 (MQ=255)
                                            gCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGACGATGAACGCATTCGCAAAAAATACATCAACGCCCGTCATTTCTTCCAGCGACTGGCAGAAATCCAGATTGAGTCCGGCTATCCATACATCATGTATg                                                                                                             >  6:375313/1‑146 (MQ=255)
                                              cGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAAACCAGTTTGAGTCCGGCTATCCCTACATCAAGTATGAAGa                                                                                                         >  4:14489/1‑148 (MQ=255)
                                                tttGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTAc                                                                                                                       >  4:131327/1‑132 (MQ=255)
                                                tttGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTAc                                                                                                                       <  3:131327/132‑1 (MQ=255)
                                                   gCCGATGTGGCCATCATCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCTCAAAAAATACATCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACgg                                                                                                    <  7:359733/148‑1 (MQ=255)
                                                   gCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACgg                                                                                                    <  8:199286/148‑1 (MQ=255)
                                                      gATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTaaa                                                                                                <  4:244959/149‑1 (MQ=255)
                                                        tgtgGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCACGCCCGTGATTTCTTACAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCg                                                                                             >  5:233390/1‑149 (MQ=255)
                                                         gtgGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTaaa                                                                                                >  8:278702/1‑146 (MQ=255)
                                                         gtgGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTaaa                                                                                                <  7:278702/146‑1 (MQ=255)
                                                                  aGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAAcc                                                                                     <  7:247690/148‑1 (MQ=255)
                                                                      aaCACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATc                                                                                >  8:335466/1‑149 (MQ=255)
                                                                      aaCACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATc                                                                                >  8:147759/1‑149 (MQ=255)
                                                                          cTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCACCGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCg                                                                            >  7:340648/1‑149 (MQ=255)
                                                                             tGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACCTCTTGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCgg                                                                           <  3:14489/147‑1 (MQ=255)
                                                                             tGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCggg                                                                          <  2:193331/148‑1 (MQ=255)
                                                                             tGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCgg                                                                           <  6:294010/147‑1 (MQ=255)
                                                                               aCGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGgcg                                                                        >  4:391888/1‑148 (MQ=255)
                                                                                 gAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCa                                                                      >  1:107367/1‑148 (MQ=255)
                                                                                    cTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATaaa                                                                  <  7:71895/149‑1 (MQ=255)
                                                                                       gTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAatat                                                               >  4:17446/1‑149 (MQ=255)
                                                                                        ttGCCGATGAACGCATTCGCAAAAAATACCTCACCGCCCGTGATTTCTTCCAGCGACTGGCAGAACTCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAatat                                                               >  6:31829/1‑148 (MQ=255)
                                                                                          gCCGATGAACGCATTCGCAAAAAATACCTCACCGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGa                                                             <  3:215315/148‑1 (MQ=255)
                                                                                          gCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGa                                                             <  1:47368/148‑1 (MQ=255)
                                                                                          gCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGa                                                             <  2:210038/148‑1 (MQ=255)
                                                                                          gCCGATGAACGCATGCGCAAAAAATACCTCAACGCCCGGGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGa                                                             <  8:340648/148‑1 (MQ=255)
                                                                                           ccGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAg                                                            >  7:92777/1‑148 (MQ=255)
                                                                                            cGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGtt                                                                                                                                          <  5:97913/69‑1 (MQ=255)
                                                                                            cGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGtt                                                                                                                                          >  6:97913/1‑69 (MQ=255)
                                                                                             gATGCACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCTTGCTAACCCTATCGCCGGGCGCATCAATATGAGTaa                                                         >  6:254842/1‑149 (MQ=255)
                                                                                             gATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTaa                                                         >  6:370960/1‑149 (MQ=255)
                                                                                                gAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAAtct                                                      >  1:368249/1‑149 (MQ=255)
                                                                                                 aaCGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCgg                                                                           >  1:213367/1‑127 (MQ=255)
                                                                                                 aaCGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCgg                                                                           <  2:213367/127‑1 (MQ=255)
                                                                                                 aaCGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAAtctc                                                     <  2:368249/149‑1 (MQ=255)
                                                                                                  aCGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTa                                                                                  >  2:361825/1‑119 (MQ=255)
                                                                                                  aCGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTa                                                                                  <  1:361825/119‑1 (MQ=255)
                                                                                                  aCGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATctct                                                    >  2:291269/1‑149 (MQ=255)
                                                                                                  aCGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCCGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATctct                                                    >  8:34919/1‑149 (MQ=255)
                                                                                                     cATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCt                                                 <  7:147759/149‑1 (MQ=255)
                                                                                                          gCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTAAGCCTACATCCTGTATGAAGACACGGTAAACCGTGCTAGCCCTATCGCCGGGCGCATAAATATGAGAAATCTCTGATCAGa                                             >  6:97717/1‑148 (MQ=255)
                                                                                                              aaaaTACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAAttt                                        <  8:111727/149‑1 (MQ=255)
                                                                                                               aaaTACCTCCACGCCCGTGATTTCTTCCAGCGACTCGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACCCGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAACtttt                                       <  2:218423/149‑1 (MQ=255)
                                                                                                               aaaTACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAAtttt                                       <  8:132702/149‑1 (MQ=255)
                                                                                                               aaaTACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGCCGATCCCAACATCATGTATGACGACACGGTAAACCGTGCTAACCCTCTCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAAtttt                                       <  6:45925/149‑1 (MQ=255)
                                                                                                               aaaTACCTCAACGCCCGTGATATCTTACAGCGACTGGCAGAAATCCAGTTTGAGTCCGTATATCCCTACATCATGTATGAAGACACGGTAAACCATGCTAACCCTATCGCCGGTCGCTTAATTATGAGTAATCTCTGCTCAGAAAtttt                                       <  5:182483/149‑1 (MQ=255)
                                                                                                                 aTACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGc                                     <  2:107367/149‑1 (MQ=255)
                                                                                                                     cTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGt                                 <  3:391888/149‑1 (MQ=255)
                                                                                                                      tCAACTCCCGTGATTACTTCCAGCGACTGGCAGAAATACAGTTTGATTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGtt                                <  4:308022/149‑1 (MQ=255)
                                                                                                                          cGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACa                            <  7:335466/149‑1 (MQ=255)
                                                                                                                            aactggATTTCTGCCAGTCGCTGGAAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGa                                                             <  5:105085/109‑1 (MQ=38)
                                                                                                                            aactggATTTCTGCCAGTCGCTGGAAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGa                                                             >  6:105085/6‑114 (MQ=38)
                                                                                                                             ccGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAgcg                         >  3:346544/1‑149 (MQ=255)
                                                                                                                               tggATTTCTGCCAGTCGCTGGAAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGcc                       >  7:361881/3‑149 (MQ=255)
                                                                                                                                tGATTTCTTCCAGCGACTGGAAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCt                      <  3:17446/149‑1 (MQ=255)
                                                                                                                                  aTTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCa                    <  7:114503/149‑1 (MQ=255)
                                                                                                                                     tcttcCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACTCGGTAACCCGTGCTAGCCCTATCGCCGGGCGCAAAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCagc                  <  2:163563/148‑2 (MQ=255)
                                                                                                                                     tcttcCAGCGACTGGCAGAAATCCAGTTTGAGTCCCGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCaga                  <  7:34919/148‑1 (MQ=255)
                                                                                                                                         ccAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCa                                    >  5:185993/1‑126 (MQ=255)
                                                                                                                                         ccAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCa                                    <  6:185993/126‑1 (MQ=255)
                                                                                                                                          cAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGa            <  8:260491/149‑1 (MQ=255)
                                                                                                                                           aGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGAc           >  2:88229/1‑149 (MQ=255)
                                                                                                                                              gACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACgag        <  5:274569/149‑1 (MQ=255)
                                                                                                                                                 tGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGc                                                                              <  7:36197/76‑1 (MQ=255)
                                                                                                                                                 tGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGc                                                                              >  8:36197/1‑76 (MQ=255)
                                                                                                                                                 tGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAAt     >  8:197574/1‑149 (MQ=255)
                                                                                                                                                 tGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAAt     >  5:418456/1‑149 (MQ=255)
                                                                                                                                                   gCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAAtct   >  8:103350/1‑149 (MQ=255)
                                                                                                                                                    cAGAAATCCAGTTTGAGTCCCGCTATCCCTACAACATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCGCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAAtctc  <  5:375313/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATGGCGCTGTTTTCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTC  >  NC_000913/2802263‑2802559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: