| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,786,146 | C→T | 6.3% | T296I (ACC→ATC) | gpmM → | phosphoglycero mutase III, cofactor‑independent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,786,146 | 0 | C | T | 6.3% | 302.7 / 6.9 | 95 | T296I (ACC→ATC) | gpmM | phosphoglycero mutase III, cofactor‑independent |
| Reads supporting (aligned to +/- strand): ref base C (45/44); new base T (3/3); total (48/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01 | |||||||||||
CCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTC > NC_000913/3785998‑3786291 | ccAGCCGCGGCAACGGAAGACGGCGATGCACTGACTTTCATGAACTTCCGTGCGGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGAc < 6:86205/149‑1 (MQ=255)ccAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGAc > 1:179194/1‑149 (MQ=255)ccAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGAc > 6:129249/1‑149 (MQ=255)ccAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGAc > 2:282441/1‑149 (MQ=255)ccAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGAc > 7:287878/1‑149 (MQ=255) aCGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGaa < 1:193179/149‑1 (MQ=255) cgcgGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGGGAAATCACTCGTTCTTTCGTGAACTCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAAt < 1:119917/149‑1 (MQ=255) cgGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATAc > 5:348627/1‑149 (MQ=255) tGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCt < 4:339379/149‑1 (MQ=255) aaGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGa > 7:221185/1‑148 (MQ=255) aGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACa < 2:28177/149‑1 (MQ=255) cGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCaaaa < 6:261402/149‑1 (MQ=255) tGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTg < 6:93827/149‑1 (MQ=255) gCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATAcgc > 3:214878/1‑131 (MQ=255) gCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATAcgc < 4:214878/131‑1 (MQ=255) cACTGATTTTCAACAACTTCCGTGCTGACCGCGCGCGGGAAATCCCTCGTGCTTTCGTGAACGATGATTTCGATGGCTTCGCGCGTAAGAAATTGGCTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGc < 2:312515/148‑1 (MQ=255) tGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCAATCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGATGACATCAAAACTTCGttt > 2:396729/1‑149 (MQ=255) aTTTTCATGGACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGc > 2:142444/1‑149 (MQ=255) aTTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTg < 7:375121/148‑1 (MQ=255) aTTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTg < 2:179194/148‑1 (MQ=255) aTTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGc > 8:402701/1‑149 (MQ=255) tCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTa < 8:201056/148‑1 (MQ=255) tCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTa < 8:50999/148‑1 (MQ=255) tCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTATCGTGAACGCTGATTTCGATGTCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGCTCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTac > 4:130503/1‑149 (MQ=255) aTGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGATCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTacc > 7:374314/1‑148 (MQ=255) tGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTaccca > 3:278130/1‑149 (MQ=255) gAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTacccac > 3:167937/1‑149 (MQ=255) gAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTacccac > 2:361634/1‑149 (MQ=255) aaCTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTacccac < 2:266651/148‑1 (MQ=255) cTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGATCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCg < 8:374314/149‑1 (MQ=255) cTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCg > 1:132059/1‑149 (MQ=255) ttCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGc > 7:134380/1‑149 (MQ=255) gTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTcc < 4:167937/149‑1 (MQ=255) gTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTcc > 6:213048/1‑149 (MQ=255) cTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCt < 1:282441/148‑1 (MQ=255) cTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCt < 4:260084/148‑1 (MQ=255) cTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTg < 5:129249/149‑1 (MQ=255) gACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGt > 6:195556/1‑149 (MQ=255) aCCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTgg < 3:77979/147‑1 (MQ=255) cgcgcgcgTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGACTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTaa < 4:170217/149‑1 (MQ=255) gcgcgcgTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGATCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTccc > 7:115933/1‑141 (MQ=255) gcgcgcgTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGATCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTccc < 8:115933/141‑1 (MQ=255) gTGAAATCACTCGTGCTTTCGTGAACGGTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGGGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTc > 4:43963/1‑149 (MQ=255) tGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCg < 5:213048/149‑1 (MQ=255) gAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCgg < 8:221185/149‑1 (MQ=255) gAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCATGGTTAACACCTTCgg > 3:46842/1‑149 (MQ=255) aaaTCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCgg > 7:245731/1‑148 (MQ=255) aaaTCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCgg < 8:245731/148‑1 (MQ=255) aaaTCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGc < 6:348627/149‑1 (MQ=255) aaaTCACTAGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGCCCGAATACGCCGCTGACATCAAAACTGCAGTTGCTTCCCCCCCCGCTTCCCTGGTTAACACCTTCGGc > 4:16754/1‑149 (MQ=255) aTCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTGCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGCCCGATTACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTACCTTGTTAACACCTTCGGc > 2:95328/1‑147 (MQ=255) tCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAg > 7:80123/1‑149 (MQ=255) tCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTGCGGCGAg > 2:288714/1‑149 (MQ=255) gTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATg < 8:134380/149‑1 (MQ=255) gCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGc > 7:369988/1‑149 (MQ=255) gTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTCCTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGACTTTGCTTACCCACCCGCTTCCCTTGTTACCACCTTCGGCGATTGGATGGCGaaaaa > 4:297654/1‑149 (MQ=255) tGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAAc > 7:307488/1‑149 (MQ=255) tGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAAc > 3:37150/1‑149 (MQ=255) gAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAAc > 7:21516/1‑148 (MQ=255) aaCGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGa < 1:396729/149‑1 (MQ=255) cTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACaaa < 2:132059/149‑1 (MQ=255) tGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACaaaa > 4:246322/1‑149 (MQ=255) aTTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACt > 1:143518/1‑149 (MQ=255) aTTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACt > 2:85218/1‑149 (MQ=255) aTTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACt < 3:43963/149‑1 (MQ=255) tttCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACt > 8:5094/1‑148 (MQ=255) tCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCa < 3:246322/148‑1 (MQ=255) gATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGtt > 7:149258/1‑149 (MQ=255) gATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGtt > 4:288427/1‑149 (MQ=255) gATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGtt < 8:369988/149‑1 (MQ=255) gATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGCTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACCAACCTCAGtt > 8:293031/1‑149 (MQ=255) gATGGCTTCGCGCGTAAGAAAGTGGGTAACGTCGATTTCGTGATGCTTACCGAATACGCCCCTGACATCAAAACTGCGGTTGCTGACCCCCCCGCTTCCCTGGGTAACACCTTCGGCGAGGGGAGGGCGAATAACGACAacacaccggt > 8:293028/1‑143 (MQ=255) aTGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTg > 7:307362/1‑149 (MQ=255) tGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGATGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGCCAAAACTCAGTTGc > 7:219180/1‑149 (MQ=255) ggCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGc < 8:149258/148‑1 (MQ=255) ggCTTCGCGAGTAAGAAAGTGGTTAACGTAGATTTCGTGATGCTGACAGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGc < 3:297654/148‑1 (MQ=255) cTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTa > 6:387090/1‑149 (MQ=255) aGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTccgaaac < 1:361634/148‑1 (MQ=255) aGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTccgaaaccgaaa < 7:10863/149‑1 (MQ=255) gtaaaCGTCGATTACGTGATGCTGACCGACTACGCCGCTGACATCAAAACTCCGGATGCTAACCCACCCGCTTCCCTGGTTAACACCTTCGGCCAGTGGATCGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGaaaaa < 1:95328/144‑1 (MQ=255) gTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAata < 5:387090/149‑1 (MQ=255) gTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCAACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAata > 7:3859/1‑149 (MQ=255) ttAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAatat > 2:172680/1‑149 (MQ=255) gTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGtt > 1:399878/1‑119 (MQ=255) gTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGtt < 2:399878/119‑1 (MQ=255) gTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCa > 8:249527/1‑149 (MQ=255) gATTTCGTGATGCTGATCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTACCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGGCCACGt < 3:130503/149‑1 (MQ=255) aTTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGtt < 7:5094/149‑1 (MQ=255) aTTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGt > 1:86020/1‑148 (MQ=255) ttCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTAc > 5:153723/1‑149 (MQ=255) tGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTttc > 3:206176/1‑149 (MQ=255) tGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTttc < 8:287878/149‑1 (MQ=255) tGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTttc < 4:37150/149‑1 (MQ=255) tGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTttc > 7:158320/1‑149 (MQ=255) aTGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGTCAAAACTCAGTTGCGTATTTCCGTAACCGAAAAATATGCCCACGTTACTttctt < 4:278130/149‑1 (MQ=255) cTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTttcttctt < 7:60010/149‑1 (MQ=255) cTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTttcttctt < 5:195556/149‑1 (MQ=255) cTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTttcttct < 3:288427/148‑1 (MQ=255) gACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTttcttcttc > 7:37679/1‑148 (MQ=255) aCCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTttcttcttc < 8:21516/147‑1 (MQ=255) | CCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTC > NC_000913/3785998‑3786291 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |