Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	NC_000913	911,086	IS2 (–) +5 bp	68.6%	intergenic (‑37/+92)	poxB ← / ← hcr	pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	381260 =	NA (NA)	46 (0.460)	39/258	NT	63.9%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	911086 =	25 (0.260)					intergenic (‑37/+96)	poxB/hcr	pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent
*	?	NC_000913	= 382590	NA (NA)	61 (0.610)	51/256	NT	71.0%	noncoding (1331/1331 nt)	IS2	repeat region
	?	NC_000913	= 911090	24 (0.250)					intergenic (‑41/+92)	poxB/hcr	pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

GATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/381407‑381260 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTAC  >  NC_000913/911086‑911229                                                                                                                                                                                                                                                                                                      GATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAT                                                                                                                                                 >  7:168584/1‑149          CTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCA                                                                                                                                         <  4:162955/148‑1          CTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCA                                                                                                                                         <  4:169119/148‑1           TGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGTGCCAGTCTATTTAGTTCATC                                                                                                                                       >  6:283872/1‑149              GTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGA                                                                                                                                    >  3:73830/1‑149               TGGTACGCCGTCTGCGTTTCTCAGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGAC                                                                                                                                   <  2:298970/149‑1                GGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACG                                                                                                                                  >  6:70962/1‑149                    CGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGG                                                                                                                              <  3:216836/149‑1                         TCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAA                                                                                                                         <  2:69333/149‑1                               TTTCTCCGTCCCTAAGACATCCATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTTTCTGGAGATGCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATG                                                                                                                   <  2:345719/149‑1                                 TCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGG                                                                                                                  <  1:158383/148‑1                                  CTCCGGCCCTCAGACATCAATCATCTGAGCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGG                                                                                                                 <  6:19824/148‑1                                     CGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGA                                                                                                              <  4:69062/148‑1                                     CGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGA                                                                                                                <  3:171141/146‑1                                                CATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCAC                                                                                                  >  8:347466/1‑149                                                CATAAATCTTCTGTGATCAAATGACTAGTCTAAAAACTCGGATTAAGACTATCACTTATTGAAGTGATATTGGTGGTCGTGAGATTCAGGGGGCCCTGCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCAC                                                                                                  <  5:283872/149‑1                                                 ATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATT                                                                                                                                                >  3:134113/1‑102                                                 ATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATT                                                                                                                                                <  4:134113/102‑1                                                      TCATCTGTTCTCCAATGACTAGTCTAACAACTAGTATTAAGACTATCACTTATTTCAGTGATATTGGTGGGCTGGAGATTCAGGCGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGG                                                                                            <  5:70962/149‑1                                                           TGTTCTCCAATGACTAGTCTAAAAACTAGAAAAAACACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGTGGGCCAGTCTAATAAGATCATCTGACGGAGGGTCAAGGGATGGGAGAGAAAGGAGGCACTAAAGGTTAAA                                                                                       >  5:139903/1‑149                                                                  CAATGACTAGTCAAAAAACTAGTATTAAGACTATCACTTCTTTAAGTGATATTGGTTGTCTGGACATGCAGGGGGCCAGACTATTTAGTTCATCTCACGGAGGGCGACGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCC                                                                                  <  5:114545/147‑1                                                                        CTACTCTAAAAACTAGTATTAAGACTCTCACTTATTTCAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCCGTCTATTTAGTTCCTCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAA                                                                          <  8:168584/149‑1                                                                              TAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATG                                                                                                                   <  2:444966/102‑1                                                                              TAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATG                                                                                                                   >  1:444966/1‑102                                                                              TAAAAACTAGTATTAAGACTATCACTTATATAAGTGATATTGGTTGTCTGGAGATTCAGGGGGACAGTCTATTTAGTAAATCTGACGGAGGGGGAAGGGAAGGGAGAGAAAGGAGGCACTAACcg                                                                                            >  3:148753/1‑123                                                                                   aaTAGTTTTAAGACTATCAGTTATTTACGTGATATTGGTTGTCTGGAGATTCACGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCC                                                                                  <  4:22892/128‑1                                                                                   ACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCC                                                                                  >  3:22892/1‑130                                                                                     TAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGTCCGATGAAAGGAATATCATCGG                                                             >  1:214347/1‑149                                                                                     TAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGTCCGATGAAAGGAATATCATCGG                                                             >  1:207878/1‑149                                                                                       GTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGG                                                             <  4:73830/147‑1                                                                                          TTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCAT                                                         >  2:163090/1‑148                                                                                          TTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAA                                                                                                          >  8:51206/1‑99                                                                                          TTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAA                                                                                                          <  7:51206/99‑1                                                                                              GACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGTGATGGGAGAGAACGGAGGCAGTAACGGATAAATAGCCCGCTGAAAGGAATATCATCGGGCATAAGGC                                                    >  6:24658/1‑149                                                                                                CTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGA                                                  >  5:77467/1‑149                                                                                                 TATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCAACTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGACCTATTAAAGGAATATCATCGGGCATAAGGCGAT                                                 >  6:133264/1‑149                                                                                                   tccCTTATTTAAGTGATATTGGCGGTCTGGAGATTCAGGGGCCCAGGCTAATTAGTTCCCCTGACGGACGGGGACGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGAT                                                 <  7:347466/144‑1                                                                                                     ACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATG                                             >  5:429747/1‑149                                                                                                       cTATTTAAGGGATCTTGGTTGGCTGGCGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGCAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAAT                                                                                      <  4:180891/105‑1                                                                                                       TTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGGAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGA                                                  >  7:401453/1‑142                                                                                                       TTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAAT                                                                                      >  3:180891/1‑106                                                                                                              AGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCAGAAGAAAGGAATATCATCGGGCAGAAGGCGATTATGCGAGAACC                                     >  2:97320/1‑148                                                                                                                 GATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAAT                                 >  1:405690/1‑149                                                                                                                          gGTCTGGAGATTCAGCGGGCCCTTCTATGTAGCTCCTCTGACGGAGGGGTAAGGGATGGGAGAGAAAGGAGGCACTAACGGGTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCGCCCTGCG                        <  6:139903/148‑1                                                                                                                               GGAGATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGG                   <  2:405690/149‑1                                                                                                                                   ATTCAGGGGGCCAGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAG                >  6:111869/1‑148                                                                                                                                       AGGGGGCAAGTCTATTAAGTTCATCTGACGTAGGGGGAAGGCATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCTTAAGCCGATTATGCGACAGCCAAAACCCCAGGCGGATGGCAGGAG             >  6:365523/1‑147                                                                                                                                        GGGGGCCAGTCTATTTAGTTCTCCTGACGGAGGGGGACGGGTTGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAG          <  6:429747/149‑1                                                                                                                                               AGTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTA   >  8:306517/1‑149                                                                                                                                                GTCTATTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTAC  >  8:421544/1‑149                                                                                                                                                                                                                                                                                                      GATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/381407‑381260 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTAC  >  NC_000913/911086‑911229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.