New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4463460 =	206 (2.050)	3 (0.030)	3/240	NT	1.6%	coding (1250/1656 nt)	treC	trehalose‑6‑P hydrolase
	?	NC_000913	4463530 =	173 (1.850)					coding (1180/1656 nt)	treC	trehalose‑6‑P hydrolase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCATGACCAACCCGCATTTCACGCGCATTACTGACTATCGCGACGTAGAGAGCCTCAATATGTTTGCCGAGCTGCGCAACGATGGGCGTGATGCCGACGAGTTATTGGCAATCCTCGCCAGTAAATCCCGTGACAACAGTCGCACGCCCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4463610‑4463460 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cacgcccatCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTT  >  NC_000913/4463530‑4463677                                                                                                                                                                                                                                                                                                             GCATGACCAACCCGCATTTCACGCGCATTACTGACTATCGCGACGTAGAGAGCCTCAATATGTTTGCCGAGCTGCGCACCGATGGGCGTGATGCCGACGAGTTATTGGCAATCCTCTCCAGTAAATCCCGTGACAACAGTCGCACGCC                                                                                                                                                         >  4:58828/1‑148            CCGCATTTCACGCGCATTACTGACTATCGCGACGTAGAGAGCCTCAATATGTTTGCCGAGCTGCGCAACGATGGGCGTGATGCCGACGAGTTATTGGCAATCCTCGCCAGTAAATCCCGTGACAACAGTCGCAC                                                                                                                                                            <  6:166257/134‑1            CCGCATTTCACGCGCATTACTGACTATCGCGACGTAGAGAGCCTCAATATGTTTGCCGAGCTGCGCAACGATGGGCGTGATGCCGACGAGTTATTGGCAATCCTCGCCAGTAAATCCCGTGACAACAGTCGCAC                                                                                                                                                            >  5:166257/1‑134                         GCATTACTGACTATCGCGACGTAGAGAGCCTCAATATGTTTGCCGAGCTGCGCAACGATGGGCGTGATGCCGACGAGTTATTGGCAATCCTCGCCAGTAAATCCCGTGACAACAGTCGCACGCCCAT                                                                                                                                                      >  6:65203/1‑127                                                                          GCGCAACGATGGGCGTGATGCCGACGAGTTATTGGCAATCCTCGCCAGTAAATCCCGTGACAACAGTCGCACGCCCATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGG                                                                               >  1:334895/1‑149                                                                              AACGATGGGCGTGATGCCGACGAGTTATTGGCAATCCTCGCCAGTAAATCCCGTGACAACAGTCGCACGCCCATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGG                                                                               <  8:460475/145‑1                                                                              AACGATGGGCGTGATGCCGACGAGTTATTGGCAATCCTCGCCAGTAAATCCCGTGACAACAGTCGCACGCCCATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGG                                                                               >  7:460475/1‑145                                                                                                                                              tccCGCCCATCGTGGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGGCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGC           <  5:309914/146‑1                                                                                                                                                ACGCCCATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCA         <  1:190153/149‑1                                                                                                                                                ACGCCCATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCA         <  8:113804/149‑1                                                                                                                                                ACGCCCATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCA         <  2:25932/149‑1                                                                                                                                                ACGCCCATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCA         >  5:406809/1‑149                                                                                                                                                ACGCCCATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGC           >  4:406660/1‑147                                                                                                                                                ACGCCCATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGC           >  2:336426/1‑147                                                                                                                                                  GCCCATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCAG        >  4:146066/1‑148                                                                                                                                                  GCCCATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCAG        >  8:381201/1‑148                                                                                                                                                  GCCCATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCAG        >  8:237134/1‑148                                                                                                                                                  GCCCATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGA                                                <  7:108199/108‑1                                                                                                                                                  GCCCATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGA                                                >  8:108199/1‑108                                                                                                                                                  GCCCATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTG                                                                            >  6:313360/1‑80                                                                                                                                                  GCCCATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTG                                                                            <  5:313360/80‑1                                                                                                                                                    CCATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCAT     >  1:52773/1‑149                                                                                                                                                    CCATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCT                                                      >  6:338216/1‑100                                                                                                                                                    CCATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCT                                                      <  5:338216/100‑1                                                                                                                                                    CCATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTC                                                           <  6:288779/95‑1                                                                                                                                                    CCATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTC                                                           >  5:288779/1‑95                                                                                                                                                     CATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCATT    >  5:335638/1‑149                                                                                                                                                      ATCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTT   >  6:153642/1‑149                                                                                                                                                       TCGTTGCGCAGCTCGTCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTT  <  5:169213/149‑1                                                                                                                                                                                                                                                                                                             GCATGACCAACCCGCATTTCACGCGCATTACTGACTATCGCGACGTAGAGAGCCTCAATATGTTTGCCGAGCTGCGCAACGATGGGCGTGATGCCGACGAGTTATTGGCAATCCTCGCCAGTAAATCCCGTGACAACAGTCGCACGCCCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4463610‑4463460 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cacgcccatCGTTGCGCAGCTCGGCAAACATATTGAGGCTCTCTACGTCGCGATAGTCAGTAATGCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTT  >  NC_000913/4463530‑4463677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.