Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,840,803 T→C 9.1% K201E (AAG→GAG)  ynjF ← CDP‑alcohol phosphatidyltransferase family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,840,8030TC 9.1% 156.9 / 5.7 55K201E (AAG→GAG) ynjFCDP‑alcohol phosphatidyltransferase family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (21/29);  new base C (3/2);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

TACGCACGCGCCATGGGTTGGAAGAATGTTTCCGTGTATGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCC  >  NC_000913/1840655‑1840950
                                                                                                                                                    |                                                                                                                                                   
tACGCACGCGCCATGGGTTGGAAGAATGTTTCCGTGTATGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGTGACtt                                                                                                                                                     <  8:417299/149‑1 (MQ=255)
      cgcgCCATGGGTTGGAAGAATGTTTCCGTGTATGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGt                                                                                                                                               <  1:456561/149‑1 (MQ=255)
        cgcCATGGGTTGGAAGAATGTTTCCGTGTATGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCTCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGAAGTCGCTCCAGCGTCa                                                                                                                                             <  5:390395/149‑1 (MQ=255)
         gcCATGGGTTGGAAGAATGTTTCCGTGTATGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAg                                                                                                                                            <  6:129447/149‑1 (MQ=255)
                 ttGGAAGAATGTTTCCGTGTATGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGAAGTCGCTCCAGCGTCa                                                                                                                                             <  6:16462/140‑1 (MQ=255)
                 ttGGAAGAATGTTTCCGTGTATGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGAAGTCGCTCCAGCGTCAGagatctg                                                                                                                                     >  5:16462/1‑142 (MQ=255)
                    gaagaaTGTTTCCGTGTATGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTc                                                                                                                                  <  5:216965/148‑1 (MQ=255)
                        aaTGTTTCCGTGTATGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCacac                                                                                                                             >  5:374115/1‑149 (MQ=255)
                          tggttCCGTGTATGACGGCGGCTGGTACGAATGGAGCAGCGAGCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACAccc                                                                                                                           <  6:343788/146‑1 (MQ=255)
                           gTTTCCGTGTATGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCcg                                                                                                                          >  2:161908/1‑149 (MQ=255)
                            tttCCGTGTATGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCcgc                                                                                                                         <  1:161908/149‑1 (MQ=255)
                               ccGTGTATGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGt                                                                                                                       <  3:30464/148‑1 (MQ=255)
                                  tgtATGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCCCACCCGCGTAAAg                                                                                                                   >  6:288208/1‑149 (MQ=255)
                                  tgtATGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAg                                                                                                                   <  2:109837/149‑1 (MQ=255)
                                    tATGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGgt                                                                                                                 >  6:452523/1‑149 (MQ=255)
                                      tGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGgtc                                                                                                                <  7:23342/148‑1 (MQ=255)
                                       gACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACAccc                                                                                                                           >  3:353819/1‑136 (MQ=255)
                                       gACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACAccc                                                                                                                           <  4:353819/136‑1 (MQ=255)
                                              gcTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAg                                                                                                       <  5:1357/149‑1 (MQ=255)
                                                  gTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGcac                                                                                                    >  5:442599/1‑148 (MQ=255)
                                                   tACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACaa                                                                                                  >  2:325760/1‑149 (MQ=255)
                                                   tACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACaa                                                                                                  >  1:437832/1‑149 (MQ=255)
                                                       aaTGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCa                                                                                              >  6:69568/1‑149 (MQ=255)
                                                       aaTGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGACCGGACAGTAGCAAATAACATTGCAATTACTGACCCTGGAGCGACTTCAGCGTCAGATACCAACTCCACACCCGCGTAAAGGTCGTCATACAGCACAACGcc                                                                                              >  1:184008/1‑148 (MQ=255)
                                                       aaTGGAGCAGCGATCCAAAAAATCCGATAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCa                                                                                              <  4:430367/149‑1 (MQ=255)
                                                            agcagcGATCCAAAAAATCCTGTAGCAACCTGTGAACGCTGCGCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGaa                                                                                         <  2:184008/149‑1 (MQ=255)
                                                             gcagcGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAg                                                                                        <  8:15071/149‑1 (MQ=255)
                                                                  gATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATcca                                                                                   <  5:69568/149‑1 (MQ=255)
                                                                   aTCCAAAAAATCCTGGACCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTTCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATccac                                                                                  <  4:241386/149‑1 (MQ=255)
                                                                   aTCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATccac                                                                                  <  1:325760/149‑1 (MQ=255)
                                                                      cAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATccacgc                                                                                >  2:37191/1‑148 (MQ=255)
                                                                       aaaaaaTCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGgtc                                                                                                                <  4:58009/115‑1 (MQ=255)
                                                                       aaaaaaTCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGgtc                                                                                                                >  6:38963/1‑115 (MQ=255)
                                                                       aaaaaaTCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGgtc                                                                                                                <  5:38963/115‑1 (MQ=255)
                                                                       aaaaaaTCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGgtc                                                                                                                >  3:58009/1‑115 (MQ=255)
                                                                       aaaaaaTCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGACGATccacgca                                                                               >  7:385872/1‑148 (MQ=255)
                                                                         aaaaTCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATccacgcaaac                                                                            <  6:374115/149‑1 (MQ=255)
                                                                            aTCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATccacgcaaaccac                                                                         <  6:442599/149‑1 (MQ=255)
                                                                                      aaCCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATg                                                               <  5:288208/149‑1 (MQ=255)
                                                                                                 gcgGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATccacgcaaaccac                                                                         <  1:425868/128‑1 (MQ=255)
                                                                                                 gcgGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATccacgcaaaccac                                                                         >  2:425868/1‑128 (MQ=255)
                                                                                                      ccGTACAGTAGCAAATAAAATTCAAATTACTGAAGAAGGAGCGAATTCATCGTCAGATACCCAATCCACACCCTCTTAAAGGTCGTCATACAGCAAAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATcc                                               <  2:84033/149‑1 (MQ=255)
                                                                                                                  aaaTAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAg                                   >  5:93193/1‑149 (MQ=255)
                                                                                                                     tAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTaa                                <  5:452523/149‑1 (MQ=255)
                                                                                                                         aTTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGt                                  >  5:103216/1‑143 (MQ=255)
                                                                                                                               aTTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGAACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCt                      <  2:437832/149‑1 (MQ=255)
                                                                                                                                 atcTGACGCTGAAGTCGCTCCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCtgt                    >  2:137599/3‑149 (MQ=255)
                                                                                                                                  tcTGACGCTGAAGTCGCTCCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCtgtg                   >  5:400127/2‑149 (MQ=255)
                                                                                                                                  aCTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCtgt                    <  1:37191/148‑1 (MQ=255)
                                                                                                                                   cTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCtgt                    <  7:393743/147‑1 (MQ=255)
                                                                                                                                   cTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCtgt                    >  8:393743/1‑147 (MQ=255)
                                                                                                                                    tGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGttt                        >  7:459057/1‑142 (MQ=255)
                                                                                                                                    tGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGttt                        <  8:459057/142‑1 (MQ=255)
                                                                                                                                     gACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCt                >  7:284180/1‑149 (MQ=255)
                                                                                                                                      aCGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATAGTTTCTGTGCCtt               >  6:36986/1‑149 (MQ=255)
                                                                                                                                            gAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAACGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTa         <  6:142256/149‑1 (MQ=255)
                                                                                                                                                   ttCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAATccccc  >  7:379183/1‑149 (MQ=255)
                                                                                                                                                   ttCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAATccccc  >  2:55682/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TACGCACGCGCCATGGGTTGGAAGAATGTTTCCGTGTATGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAACATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCC  >  NC_000913/1840655‑1840950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑