Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,936,091 T→C 5.3% D75G (GAT→GGT)  zwf ← glucose‑6‑phosphate 1‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,936,0910TC 5.3% 351.0 / 4.7 111D75G (GAT→GGT) zwfglucose‑6‑phosphate 1‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (53/52);  new base C (3/3);  total (56/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

GTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGC  >  NC_000913/1935947‑1936237
                                                                                                                                                |                                                                                                                                                  
    tGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGtt                                                                                                                                            >  7:300234/1‑149 (MQ=255)
         ggcATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATTCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGtttcttt                                                                                                                                       <  3:228957/149‑1 (MQ=255)
          gcATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGtttctttc                                                                                                                                      <  6:450614/149‑1 (MQ=255)
            aTGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCAt                                                                                                                                    >  5:336884/1‑149 (MQ=255)
              ggCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATg                                                                                                                                   >  3:352868/1‑148 (MQ=255)
                cAAAGAAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGa                                                                                                                                  >  6:358740/1‑147 (MQ=255)
                   agtagtTTATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGtt                                                                                                                             <  4:40804/149‑1 (MQ=255)
                       gtTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTc                                                                                                                           >  4:204801/1‑147 (MQ=255)
                       gtTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTc                                                                                                                           >  2:460789/1‑147 (MQ=255)
                       gtTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTc                                                                                                                           >  6:379667/1‑147 (MQ=255)
                       gtTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGa                                                                                                                         <  3:379128/149‑1 (MQ=255)
                        ttAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAg                                                                                                                        <  3:183866/149‑1 (MQ=255)
                          aaTGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAgcg                                                                                                                      >  4:343518/1‑149 (MQ=255)
                          aaTGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAgc                                                                                                                       <  5:379667/148‑1 (MQ=255)
                          aaTGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAgc                                                                                                                       <  5:284253/148‑1 (MQ=255)
                              gTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTc                                                                                                                  >  5:96401/1‑149 (MQ=255)
                                 aTACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTcgcg                                                                                                               <  5:221942/149‑1 (MQ=255)
                                     gATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATAACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGAc                                                                                                            >  6:162333/1‑148 (MQ=255)
                                       tttttttGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGTGATTACAAAAATCCAGACGTGCACTCAGGTTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCTCCTCGCGGCCAAc                                                                                                         >  7:104332/1‑149 (MQ=255)
                                              gATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGt                                                                                                   <  5:358740/148‑1 (MQ=255)
                                               aTCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGta                                                                                                  >  8:408800/1‑148 (MQ=255)
                                                tCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGtata                                                                                                >  1:388013/1‑149 (MQ=255)
                                                tCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGtata                                                                                                >  4:125993/1‑149 (MQ=255)
                                                 ccAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTGTTTCATGaaa                                                                                                                                <  7:351195/116‑1 (MQ=255)
                                                  cAGCATCGCGCAGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATg                                                                                              >  2:225756/1‑149 (MQ=255)
                                                           gcCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTAt                                                                                     <  8:300234/149‑1 (MQ=255)
                                                               agaCGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCa                                                                                 <  5:168773/149‑1 (MQ=255)
                                                               agaCGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTAATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCTGACAACTTTGGTATATGCCGCTTTATCCCa                                                                                 <  5:154382/149‑1 (MQ=255)
                                                                    gCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAg                                                                            <  7:416356/149‑1 (MQ=255)
                                                                     cTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCttt                                                                                       >  4:365657/1‑137 (MQ=255)
                                                                     cTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCttt                                                                                       <  3:365657/137‑1 (MQ=255)
                                                                          tGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGcc                                                                      >  2:295571/1‑149 (MQ=255)
                                                                           gcagcaGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGccc                                                                     >  6:184172/1‑149 (MQ=255)
                                                                                agTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGc                                                                >  8:302596/1‑149 (MQ=255)
                                                                                    tCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGa                                                             >  5:440829/1‑148 (MQ=255)
                                                                                    tCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGa                                                             <  7:146160/148‑1 (MQ=255)
                                                                                    tCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGa                                                             >  2:443403/1‑148 (MQ=255)
                                                                                      aTTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATaa                                                          >  1:466121/1‑149 (MQ=255)
                                                                                      aTTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATa                                                           >  2:194261/1‑148 (MQ=255)
                                                                                      aTTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCACCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATaa                                                          >  7:264366/1‑149 (MQ=255)
                                                                                      aTTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCACCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATaa                                                          >  7:186438/1‑149 (MQ=255)
                                                                                      aTTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCACCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATaa                                                          >  7:180026/1‑149 (MQ=255)
                                                                                          aCATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTAc                                                                  <  4:383917/137‑1 (MQ=255)
                                                                                          aCATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCg                                                      >  5:1320/1‑149 (MQ=255)
                                                                                           cATCGAGATTACAAAAATCCAGACGTGCACTCAGTGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCgg                                                     <  2:466121/149‑1 (MQ=255)
                                                                                           cATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGa                                                             <  3:26343/141‑1 (MQ=255)
                                                                                           cATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCgg                                                     <  1:194261/149‑1 (MQ=255)
                                                                                           cATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCgg                                                     <  1:443403/149‑1 (MQ=255)
                                                                                           cATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCgg                                                     <  3:125993/149‑1 (MQ=255)
                                                                                            aTCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCggg                                                    >  8:356234/1‑149 (MQ=255)
                                                                                               gagaTTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGgtgt                                                 >  3:250250/1‑149 (MQ=255)
                                                                                                 gaTTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTcc                                               >  3:419454/1‑149 (MQ=255)
                                                                                                     aCAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCAGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCggg                                            <  2:388013/148‑1 (MQ=255)
                                                                                                      cAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGtt                                          >  8:126658/1‑149 (MQ=255)
                                                                                                       aaaaaTCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGtt                                          >  6:336594/1‑148 (MQ=255)
                                                                                                       aaaaaTCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTg                                         >  1:303359/1‑149 (MQ=255)
                                                                                                         aaaTCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAg                                       <  7:356234/149‑1 (MQ=255)
                                                                                                         aaaTCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAg                                       <  4:250250/149‑1 (MQ=255)
                                                                                                         aaaTCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAg                                       <  7:302596/149‑1 (MQ=255)
                                                                                                          cacccAGACGTGCACTCAGCGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGc                                      <  4:352868/146‑1 (MQ=255)
                                                                                                          aaTCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGc                                      <  8:48994/149‑1 (MQ=255)
                                                                                                          aaTCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGc                                      >  4:430357/1‑149 (MQ=255)
                                                                                                           aTCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCt                                     >  8:317562/1‑149 (MQ=255)
                                                                                                              cAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGAc                                  <  6:96401/149‑1 (MQ=255)
                                                                                                                 aCGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCgg                               >  5:256693/1‑149 (MQ=255)
                                                                                                                        cTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTc                        <  3:204801/149‑1 (MQ=255)
                                                                                                                        cTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTc                        >  7:430611/1‑149 (MQ=255)
                                                                                                                         tCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTc                        >  5:174887/1‑148 (MQ=255)
                                                                                                                         tCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTc                        >  6:426546/1‑148 (MQ=255)
                                                                                                                         tCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTc                        >  7:352501/1‑148 (MQ=255)
                                                                                                                         tCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTc                        >  2:8052/1‑148 (MQ=255)
                                                                                                                           aGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCgg                                                     <  2:60285/117‑1 (MQ=255)
                                                                                                                           aGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCgg                                                     >  1:60285/1‑117 (MQ=255)
                                                                                                                            gggTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGt                    <  6:1320/149‑1 (MQ=255)
                                                                                                                             ggTGTCCCATAAACCTTCACCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGtt                   <  8:264366/149‑1 (MQ=255)
                                                                                                                             ggTGTCCCATAAACCTTCACCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGtt                   <  8:186438/149‑1 (MQ=255)
                                                                                                                             ggTGTACCATACACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGtt                   <  3:299368/149‑1 (MQ=255)
                                                                                                                                 tCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATa               <  1:225756/149‑1 (MQ=255)
                                                                                                                                   ccATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGa                                        >  3:379175/1‑122 (MQ=255)
                                                                                                                                   ccATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGa                                        <  4:379175/122‑1 (MQ=255)
                                                                                                                                   ccATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTACGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGtt                   >  4:232826/1‑143 (MQ=255)
                                                                                                                                    cATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATa               >  4:184328/1‑146 (MQ=255)
                                                                                                                                    cATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACa             <  3:184328/148‑1 (MQ=255)
                                                                                                                                    cATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAg            >  8:170974/1‑149 (MQ=255)
                                                                                                                                     ttAAACCTTCATCAATGGTTTCTTTAATTAAAGTTTCGATCTCCTCGAGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCTCGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCTGCTTTTTCCAGTTGATACAgg           <  8:104332/148‑1 (MQ=255)
                                                                                                                                     aTAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCg                                              >  2:15071/1‑114 (MQ=255)
                                                                                                                                     aTAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCg                                              <  1:15071/114‑1 (MQ=255)
                                                                                                                                     aTAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAgg           <  8:430611/149‑1 (MQ=255)
                                                                                                                                       aaaCCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAggg          >  5:320019/1‑148 (MQ=255)
                                                                                                                                        aaacTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGaa        <  7:381228/146‑1 (MQ=255)
                                                                                                                                          ccTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAgg      >  5:437887/1‑149 (MQ=255)
                                                                                                                                           cTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAgg      >  8:311120/1‑148 (MQ=255)
                                                                                                                                           cTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGgc     <  6:336884/149‑1 (MQ=255)
                                                                                                                                           cTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGgc     <  6:437887/149‑1 (MQ=255)
                                                                                                                                            ttCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAgg           >  1:375629/1‑142 (MQ=255)
                                                                                                                                            ttCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAgg           <  2:375629/142‑1 (MQ=255)
                                                                                                                                             tcatcaATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAgg      >  7:359344/1‑146 (MQ=255)
                                                                                                                                             tcatcaATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGgcag   >  8:394653/1‑149 (MQ=255)
                                                                                                                                             tcatcaATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGgca    <  8:359344/148‑1 (MQ=255)
                                                                                                                                              catcaATGGTTGCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGgca    <  5:162333/147‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGC  >  NC_000913/1935947‑1936237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: