Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,981,762 A→C 5.1% V209G (GTT→GGT)  otsB ← trehalose‑6‑phosphate phosphatase, biosynthetic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,981,7620AC 5.1% 356.7 / 5.6 111V209G (GTT→GGT) otsBtrehalose‑6‑phosphate phosphatase, biosynthetic
Reads supporting (aligned to +/- strand):  ref base A (51/54);  new base C (3/3);  total (54/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

TCACTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGC  >  NC_000913/1981618‑1981908
                                                                                                                                                |                                                                                                                                                  
tcaCTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGAc                                                                                                                                                >  2:235398/1‑149 (MQ=255)
    tCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACt                                                                                                                                               >  5:67623/1‑146 (MQ=255)
    tCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACt                                                                                                                                               <  6:67623/146‑1 (MQ=255)
      cTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGaa                                                                                                                                          <  2:69832/149‑1 (MQ=255)
       tGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAg                                                                                                                                         >  2:304567/1‑149 (MQ=255)
         ttATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGc                                                                                                                                        >  5:164460/1‑148 (MQ=255)
           aTTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCa                                                                                                                                      <  6:138371/148‑1 (MQ=255)
            ttttCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGa                                                                                                                                    <  6:94699/149‑1 (MQ=255)
            ttttCTCTTTTTTGTTGTAAGGCGGTGGTGATCATTTCTCGCCAGCTCCAGACAGCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTAACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAg                                                                                                                                     <  5:21226/148‑1 (MQ=255)
               tctcTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGAtt                                                                                                                                  >  3:364470/1‑148 (MQ=255)
               tctcTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGAtt                                                                                                                                  >  7:457695/1‑148 (MQ=255)
                   ttttttGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCAt                                                                                                                               <  2:290670/147‑1 (MQ=255)
                    tttttGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGAGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCgg                                                                                                                            >  4:238861/1‑149 (MQ=255)
                     ttttGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATc                                                                                                                              <  7:65385/146‑1 (MQ=255)
                     ttttGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCg                                                                                                                             <  8:457695/147‑1 (MQ=255)
                     ttttGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGGCGGTTAACGACTGCGAAGCCAGATTCATc                                                                                                                              <  7:285026/146‑1 (MQ=255)
                      tttGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGt                                                                                                                           >  6:171336/1‑148 (MQ=255)
                      tttGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGt                                                                                                                           <  7:298885/148‑1 (MQ=255)
                          ttgtAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTAATAGGTTAAat                                                                                                                      >  5:231242/1‑149 (MQ=255)
                            gtAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAatca                                                                                                                    >  7:202636/1‑149 (MQ=255)
                               aTGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAatcatc                                                                                                                  <  3:110100/148‑1 (MQ=255)
                                       gtTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGaaa                                                                                                         >  3:123524/1‑149 (MQ=255)
                                        ttATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGaaa                                                                                                         >  4:251328/1‑148 (MQ=255)
                                          ataaTTTAAATCAAGCTCCAGACATCCTGCACACCCGCCAGTCTCCATGATGACTGAGTTGCAACTGTGCCAATTTTTACTGACATTCAGACCAGTCGGTTAACGACTGCGAAGCCAGATTCAta                                                                                                                              >  1:121316/4‑124 (MQ=255)
                                             aTTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACggg                                                                                                   >  8:15973/1‑149 (MQ=255)
                                                 cAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGt                                                                                                <  6:164460/148‑1 (MQ=255)
                                                  aaGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTc                                                                                              <  2:445993/149‑1 (MQ=255)
                                                       aGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGccc                                                                                          >  2:93824/1‑148 (MQ=255)
                                                             aGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAg                                                                                   >  6:182430/1‑149 (MQ=255)
                                                             aGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGAGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAg                                                                                   <  3:238861/149‑1 (MQ=255)
                                                              gACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAgg                                                                                  <  1:235398/149‑1 (MQ=255)
                                                                 aTCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCTAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCg                                                                                                                 >  1:448590/1‑115 (MQ=255)
                                                                  tCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGc                                                                              <  1:203233/149‑1 (MQ=255)
                                                                   ccGGCACACCCGCCAGTCGCCGTGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAAc                                                                                                                                                   >  7:470027/1‑79 (MQ=255)
                                                                   ccGGCACACCCGCCAGTCGCCGTGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAAc                                                                                                                                                   <  8:470027/79‑1 (MQ=255)
                                                                        acacCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTg                                                                        <  8:387715/149‑1 (MQ=255)
                                                                        acacCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTg                                                                        <  1:304567/149‑1 (MQ=255)
                                                                        acacCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTg                                                                        <  5:171336/149‑1 (MQ=255)
                                                                         cacCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTg                                                                        >  4:360087/1‑148 (MQ=255)
                                                                           cccGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCa                                                                      >  2:232795/1‑148 (MQ=255)
                                                                             cGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGAGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATa                                                                    >  7:404146/1‑148 (MQ=255)
                                                                              gCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATaa                                                                   >  1:147633/1‑148 (MQ=255)
                                                                               ccAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATaaaa                                                                 <  8:202636/149‑1 (MQ=255)
                                                                               ccAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATaaaa                                                                 <  6:117622/149‑1 (MQ=255)
                                                                                   tCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACgg                                                                                                    <  2:471727/110‑1 (MQ=255)
                                                                                   tCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTg                                                             <  1:301984/149‑1 (MQ=255)
                                                                                   tCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTg                                                             <  6:177675/149‑1 (MQ=255)
                                                                                      ccATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCaa                                                          >  5:310997/1‑149 (MQ=255)
                                                                                      ccATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCaa                                                          >  7:141379/1‑149 (MQ=255)
                                                                                      ccATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCaa                                                          >  1:57025/1‑149 (MQ=255)
                                                                                       cATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAAt                                                         >  8:432995/1‑149 (MQ=255)
                                                                                       cATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAAt                                                         >  6:153759/1‑149 (MQ=255)
                                                                                           atgCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGc                                                      <  2:57025/148‑1 (MQ=255)
                                                                                               cTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGc                                                      >  8:153317/1‑144 (MQ=255)
                                                                                               cTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGc                                                      <  7:153317/144‑1 (MQ=255)
                                                                                               cTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCAGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCAc                                                 <  2:170852/149‑1 (MQ=255)
                                                                                                  aGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCAcc                                                <  4:364470/147‑1 (MQ=255)
                                                                                                   gTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAgg                                                                                  <  6:121359/112‑1 (MQ=255)
                                                                                                    ttGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTa                                            <  3:360087/149‑1 (MQ=255)
                                                                                                     tgaaCCTGTGACAATTTTTAATGACAATCAGCCCAGTGGTTTACCGACTGCGTAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTTCAATTGCCTCACCTTTAc                                           <  2:362845/146‑1 (MQ=255)
                                                                                                      gCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACt                                          >  2:169255/1‑149 (MQ=255)
                                                                                                       cACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTg                                         <  7:15973/149‑1 (MQ=255)
                                                                                                          cTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCt                                                              <  3:336399/125‑1 (MQ=255)
                                                                                                          cTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCt                                                              >  4:336399/1‑125 (MQ=255)
                                                                                                          cTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTa                                      >  1:229328/1‑149 (MQ=255)
                                                                                                          cTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTa                                      >  5:59981/1‑149 (MQ=255)
                                                                                                             tgCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTAc                                     <  1:232795/147‑1 (MQ=255)
                                                                                                               ccAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTCCAATTGCCTCACCTTTACTGGTACcact                                 >  4:211484/1‑149 (MQ=255)
                                                                                                                cAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGc                                                            >  1:328469/1‑121 (MQ=255)
                                                                                                                cAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGc                                                            <  2:328469/121‑1 (MQ=255)
                                                                                                                cAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCtctc                                >  8:45101/1‑149 (MQ=255)
                                                                                                                cAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCtctc                                >  6:368577/1‑149 (MQ=255)
                                                                                                                    ttttACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGtt                            >  1:468120/1‑149 (MQ=255)
                                                                                                                     tttACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGtt                            <  2:468120/148‑1 (MQ=255)
                                                                                                                      ttACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTAATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGATGCAATTGCCTCACCTTTACTGGTACCTCTCGGttt                           <  8:404146/148‑1 (MQ=255)
                                                                                                                       tACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTg                          <  8:395211/148‑1 (MQ=255)
                                                                                                                          tGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCttt                                             >  5:56756/1‑126 (MQ=255)
                                                                                                                          tGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCttt                                             <  6:56756/126‑1 (MQ=255)
                                                                                                                          tGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTa                                            >  6:61178/1‑127 (MQ=255)
                                                                                                                          tGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTa                                            <  5:61178/127‑1 (MQ=255)
                                                                                                                          tGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTGTGAgct                      >  5:339302/1‑149 (MQ=255)
                                                                                                                            aCATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAtctc                     <  3:251328/148‑1 (MQ=255)
                                                                                                                            aCATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAtctc                     <  1:93824/148‑1 (MQ=255)
                                                                                                                            aCATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAtct                      <  5:182430/147‑1 (MQ=255)
                                                                                                                                tCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCCTCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGCGCTTCCTGCCTAAAAGCTGCAATTGCCTCCCCTTTACTGGTACCTCTCGGTTTGATCGCGacaa                >  3:7004/1‑149 (MQ=255)
                                                                                                                                tCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAAtt                                                        >  6:374051/1‑109 (MQ=255)
                                                                                                                                tCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAAtt                                                        <  5:374051/109‑1 (MQ=255)
                                                                                                                                  ttcgcAGTCGTTAACCGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTa                                            >  5:186732/5‑119 (MQ=255)
                                                                                                                                  ttcgcAGTCGTTAACCGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTa                                            <  6:186732/115‑1 (MQ=255)
                                                                                                                                  ttcgcAGTCGTTAACCGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTa                                            >  5:180120/5‑119 (MQ=255)
                                                                                                                                  ttcgcAGTCGTTAACCGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGacaaca              >  5:183475/5‑149 (MQ=255)
                                                                                                                                   gCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAacac             >  1:201438/1‑149 (MQ=255)
                                                                                                                                        gTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACttt        <  2:229328/149‑1 (MQ=255)
                                                                                                                                         tCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGac                  >  4:93942/1‑138 (MQ=255)
                                                                                                                                         tCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGac                  <  3:93942/138‑1 (MQ=255)
                                                                                                                                           ggTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTcc      <  1:169255/148‑1 (MQ=255)
                                                                                                                                           ggTTAACGACTGCGAAGCCAGATTCATCGGGTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTAACCTTTACTGGTACCTCTCGGTTTGATCTCGAAAACAAACTTTccc     >  2:419746/1‑149 (MQ=255)
                                                                                                                                               aaCGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCctgc  <  2:201438/148‑1 (MQ=255)
                                                                                                                                                aCGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGa                   >  1:414768/1‑130 (MQ=255)
                                                                                                                                                aCGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGa                   <  2:414768/130‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
TCACTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGC  >  NC_000913/1981618‑1981908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: