Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,987,857 C→T 6.6% intergenic (‑99/+16) yecJ ← / ← azuC DUF2766 family protein/acid‑inducible small membrane‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,987,8570CT 6.6% 327.4 / 8.0 106intergenic (‑99/+16)yecJ/azuCDUF2766 family protein/acid‑inducible small membrane‑associated protein
Reads supporting (aligned to +/- strand):  ref base C (48/51);  new base T (3/4);  total (51/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01

Only 100 of 106 total aligned reads displayed.

ACCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTT  >  NC_000913/1987712‑1988001
                                                                                                                                                 |                                                                                                                                                
aCCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGa                                                                                                                                               <  1:130902/149‑1 (MQ=255)
   acaTCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCg                                                                                                                                                 <  7:428250/144‑1 (MQ=255)
   acaTCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCg                                                                                                                                                 >  8:428250/1‑144 (MQ=255)
   acaTCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAg                                                                                                                                              >  2:446859/1‑147 (MQ=255)
      tCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCGTGATAGCATTTTGAAAGCCGTTATAGTATCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGAAGGt                                                                                                                                         >  4:253699/1‑149 (MQ=255)
         gAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGtttt                                                                                                                                      >  3:382540/1‑149 (MQ=255)
         gAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGtttt                                                                                                                                      >  8:317850/1‑149 (MQ=255)
          aaaCCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCTCCGGCAGGttttt                                                                                                                                     <  6:422132/149‑1 (MQ=255)
          aaaCCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGttttt                                                                                                                                     >  7:460777/1‑149 (MQ=255)
           aaCCAATTCCTGTTCGGCATTCAGCGGCTGTGACATCTTAAACTCCTGATATCATTTTGTAAGGCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGtttttt                                                                                                                                    <  5:2565/149‑1 (MQ=255)
           aaCCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGtttttt                                                                                                                                    <  3:345373/149‑1 (MQ=255)
            aCCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCACTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCTCCGGCAGGttttttt                                                                                                                                   <  8:38036/149‑2 (MQ=255)
             ccAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGt                                                                                                                                  >  7:383838/1‑149 (MQ=255)
              cAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGc                                                                                                                                             >  6:159072/1‑137 (MQ=255)
              cAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGc                                                                                                                                             <  5:159072/137‑1 (MQ=255)
              cAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGt                                                                                                                                  >  5:133720/1‑148 (MQ=255)
               aaTTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTa                                                                                                                                >  1:37710/1‑149 (MQ=255)
                aTTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTAt                                                                                                                               >  5:327264/1‑149 (MQ=255)
                 ttCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATc                                                                                                                              <  5:390506/149‑1 (MQ=255)
                    cTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCgg                                                                                                                            <  6:80446/148‑1 (MQ=255)
                     tGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGaa                                                                                                                          >  7:310419/1‑149 (MQ=255)
                          ggCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACatat                                                                                                                     >  2:344279/1‑149 (MQ=255)
                          ggCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACatat                                                                                                                     >  8:183459/1‑149 (MQ=255)
                             aTTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGc                                                                                                                  <  4:422248/149‑1 (MQ=255)
                                 aGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTgg                                                                                                              <  4:382540/149‑1 (MQ=255)
                                  gCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGc                                                                                                             >  6:91368/1‑149 (MQ=255)
                                   cGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCg                                                                                                            <  8:121693/149‑1 (MQ=255)
                                   cGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCg                                                                                                            <  8:460777/149‑1 (MQ=255)
                                     gCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAAACTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCgg                                                                                                           >  2:30768/1‑148 (MQ=255)
                                       tGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCATCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTAc                                                                                                        >  2:163310/1‑149 (MQ=255)
                                           aCATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAGCCTGCCGGAGCAGGTTTTTTGTTATc                                                                                                                              <  8:167021/123‑1 (MQ=255)
                                           aCATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAGCCTGCCGGAGCAGGTTTTTTGTTATc                                                                                                                              >  7:167021/1‑123 (MQ=255)
                                           aCATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCt                                                                                                    <  1:335478/149‑1 (MQ=255)
                                                gTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGCCGTCACATCGTCCGCGAAAGTCACGTCCACCGTCATCACGCCACAAAAAACCCTGCCCGCGCAGGTTTTTGGTTAGCGGCACATATTGCCTGGCGGTCCGTCtgttca                                                                                               >  4:281688/1‑144 (MQ=255)
                                                gTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGa                                                                                                <  1:344279/148‑1 (MQ=255)
                                                 tAAACTCGTGATAGCATTTTGAAAGCCGATATAGTCGCGACTTCACATCTTCAGAGATAGTCACATCCACCGTCATCAGCACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGGCTTTGAAc                                                                                              <  1:108512/149‑1 (MQ=255)
                                                 tAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCTACTTCACATCTTCAGCGAAAGTCACATCCACCGTCATCAGTACACAAAAAAACCTGCCGGAGAAGGATTTTTGTTATCGGAACATATTGCCTCGCCGTACGTCTTTGAAc                                                                                              >  3:341875/1‑149 (MQ=255)
                                                 tAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAAc                                                                                              >  7:209172/1‑149 (MQ=255)
                                                    aCTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACAGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTc                                                                                           <  6:197107/149‑1 (MQ=255)
                                                     cTCCTGATAGCATTTTGAAAGCCTTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCt                                                                                          <  7:357381/149‑1 (MQ=255)
                                                     cTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCt                                                                                          <  3:344612/149‑1 (MQ=255)
                                                      tCCTGATAGCATTTTGAAAGCCGTTATAGTAGAGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCtt                                                                                         >  2:441694/1‑149 (MQ=255)
                                                        cTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTg                                                                                        <  2:260259/148‑1 (MQ=255)
                                                        cTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGc                                                                                       <  6:133720/149‑1 (MQ=255)
                                                               cATTTTGAACGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGt                                                                                 >  2:160604/1‑148 (MQ=255)
                                                                aTTTTGAAAGACGGTATAGTATAGTCTTCACATCTTCAGCGATAGTCACATCCACCGTCATCCGGACACAAAAAAACCTTCCGGAGCAGGTTTTTTGTTATCTGAACATATTGCCTGGCGGTAAGTCTTTGAACGTATTGCAATAGTTa                                                                               <  8:233838/149‑1 (MQ=255)
                                                                 ttttGAAAGCCCTTATAGTAGCGACTTCACATCTTAAGCGAAAGTCACATCCAACGTCATCAGGACACAAAAAAACCTGCCGGAGAAGGTTTTTAGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTAt                                                                              >  8:150139/1‑149 (MQ=255)
                                                                     gAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAgg                                                                                                                                          >  1:460517/1‑85 (MQ=255)
                                                                     gAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAgg                                                                                                                                          <  2:460517/85‑1 (MQ=255)
                                                                           cGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACt                                                                    >  3:427459/1‑149 (MQ=255)
                                                                            gTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACtt                                                                   <  6:280974/149‑1 (MQ=255)
                                                                                tagtagCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCa                                                               <  1:441694/149‑1 (MQ=255)
                                                                                       gACTTCAGATCTTCAGCGTTAGTCACATCCACCGTCATCAGGACACAAAAAAACCTTCCTGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGAtttt                                                        <  1:30768/149‑1 (MQ=255)
                                                                                         cTTCACATCTTCTGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTgc                                                      <  3:281688/149‑1 (MQ=255)
                                                                                         cTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTgc                                                      <  8:383838/149‑1 (MQ=255)
                                                                                         cTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTgc                                                      <  1:266943/149‑1 (MQ=255)
                                                                                           tCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTgcg                                                     >  7:16677/1‑148 (MQ=255)
                                                                                             acaTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATCTTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAg                                                  >  6:23530/1‑149 (MQ=255)
                                                                                             acaTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCa                                                   <  2:37710/148‑1 (MQ=255)
                                                                                               aTCTTCAGCGATAGTCACATCCATCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGtt                                                <  1:163310/149‑1 (MQ=255)
                                                                                               aTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGtt                                                >  6:153852/1‑149 (MQ=255)
                                                                                                tCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGttt                                               >  5:283406/1‑149 (MQ=255)
                                                                                                tCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGtt                                                >  8:415235/1‑148 (MQ=255)
                                                                                                 cTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAg                                                              >  1:466846/1‑133 (MQ=255)
                                                                                                 cTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAg                                                              <  2:466846/133‑1 (MQ=255)
                                                                                                   tCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGACTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCAt                                            >  7:247898/1‑149 (MQ=255)
                                                                                                                  tCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGAc                              >  2:260095/1‑148 (MQ=255)
                                                                                                                      ccGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCttt                                                                                                  <  4:421978/76‑1 (MQ=255)
                                                                                                                      ccGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCttt                                                                                                  >  3:421978/1‑76 (MQ=255)
                                                                                                                      ccGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCAttt                         <  7:183459/149‑1 (MQ=255)
                                                                                                                       cGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTg                        <  5:91368/149‑1 (MQ=255)
                                                                                                                         tcatcaGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGtt                      <  8:16677/149‑1 (MQ=255)
                                                                                                                           atcaGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTta                     <  7:141712/148‑1 (MQ=255)
                                                                                                                           atcaGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTta                     <  1:446859/148‑1 (MQ=255)
                                                                                                                           atcaGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTta                     <  6:327264/148‑1 (MQ=255)
                                                                                                                            tcaGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCGTCGCGGCACTCCGACCATTTGTtata                   <  4:43473/149‑1 (MQ=255)
                                                                                                                            tcaGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTtata                   <  4:427459/149‑1 (MQ=255)
                                                                                                                            tcaGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTtata                   >  2:464877/1‑149 (MQ=255)
                                                                                                                              aGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGACCATATTGGCTTGCGGTACGTCTTTGATCGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATAc                  <  4:341875/148‑1 (MQ=255)
                                                                                                                              aGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGACCATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTtata                   >  5:83452/1‑147 (MQ=255)
                                                                                                                              aGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACa                 <  4:382487/148‑1 (MQ=255)
                                                                                                                               ggACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAg                <  8:310419/149‑1 (MQ=255)
                                                                                                                                  cacaAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTGCATCGCGGCACTCCGACCATTTGTTATACAGgtg             <  1:260095/149‑1 (MQ=255)
                                                                                                                                   acaAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGgtgt            >  1:241316/1‑149 (MQ=255)
                                                                                                                                    caAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGgt              <  7:145278/146‑1 (MQ=255)
                                                                                                                                    caAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGgt              >  8:145278/1‑146 (MQ=255)
                                                                                                                                     aacaacaCCTGCCGTAGCAGGTTTTTTGTTATCGGAACATATTGCAGGGCGGTACGTCGTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGTATTTTGCGCAGTTTCATCTCGGCACTCAGACCATTTTTTATACAg                <  6:237488/137‑1 (MQ=255)
                                                                                                                                     aaaaaaaCCTGCCGGAGCATGGTTTTTGTTATCGGAACATATTGCCTGGCGGTCCGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACAATTTGTTATACAg                >  5:237488/1‑143 (MQ=255)
                                                                                                                                      aaaaaaCCTGCTCCGGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGtt                      >  4:188628/1‑136 (MQ=255)
                                                                                                                                      aaaaaaCCTGCTCCGGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGtt                      <  3:188628/136‑1 (MQ=255)
                                                                                                                                      aaaaaaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTAt         >  2:459774/1‑149 (MQ=255)
                                                                                                                                      aaaaaaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTAt         >  2:452432/1‑149 (MQ=255)
                                                                                                                                      aaaaaaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTAt         <  1:160604/149‑1 (MQ=255)
                                                                                                                                       aaaaaCCTGCTCCGGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCATTAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTAtt        >  5:244418/1‑149 (MQ=255)
                                                                                                                                          aaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTc     <  7:317850/149‑1 (MQ=255)
                                                                                                                                          aaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTc     >  8:79204/1‑149 (MQ=255)
                                                                                                                                            ccTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCtt   >  4:264584/1‑149 (MQ=255)
                                                                                                                                             cTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCttt  <  7:415235/149‑1 (MQ=255)
                                                                                                                                               gCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTAtt        >  8:19003/1‑141 (MQ=255)
                                                                                                                                               gCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTAtt        <  7:19003/141‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTT  >  NC_000913/1987712‑1988001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: