Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,120,198 T→C 5.2% T453A (ACG→GCG)  wcaJ ← colanic biosynthesis UDP‑glucose lipid carrier transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,120,1980TC 5.2% 240.6 / 2.6 76T453A (ACG→GCG) wcaJcolanic biosynthesis UDP‑glucose lipid carrier transferase
Reads supporting (aligned to +/- strand):  ref base T (32/40);  new base C (2/2);  total (34/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

GATAATCCGCGCCAGCACGGTCATCTGCACCAGCCCGAGGCCGATGATGATCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCAC  >  NC_000913/2120051‑2120345
                                                                                                                                                   |                                                                                                                                                   
gATAATCCGCGCCAGCACGGTCATCTGCACCAGCCCGAGGCCGATGATGATCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGt                                                                                                                                                     >  1:182027/1‑148 (MQ=255)
gATAATCCGCGCCAGCACGGTCATCTGCACCAGCCCGAGGCCGATGATGATCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGt                                                                                                                                                     >  6:173207/1‑148 (MQ=255)
 aTAATCCGCGCCAGCACGGTCATCTGCACCAGCCCGAGGCCGATGATGATCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCa                                                                                                                                                   <  1:331078/149‑1 (MQ=255)
 aTAATCCGCGCCAGCACGGTCATCTGCACCAGCCCGAGGCCGATGATGATCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTGTTTTCACGTAAGCTCATATCAATATGCCGCGTTGTTAACGAAACCTTTGAACACCGTCa                                                                                                                                                   <  2:357703/149‑1 (MQ=255)
           ccAGCACGGTCATCTGCACCAGCCCGAGGCCGATGATAATCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGAt                                                                                                                                         <  8:399098/149‑1 (MQ=255)
            cAGCACGGTCATCTGCACCAGCCCGATGGCGATGATGATCACAGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGAtt                                                                                                                                        <  2:134377/149‑1 (MQ=255)
            cAGCACGGTCATCTGCACCAGCCCGAGGCCGATGATGATCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGAtt                                                                                                                                        >  6:116133/1‑149 (MQ=255)
             aGCACGGTCATCTGCACCAGCCCGAGGCCGATGATGATCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGAttt                                                                                                                                       <  6:78903/149‑1 (MQ=255)
              gCACGGTCATCTGCACCAGCCCGAGGCCGATGATGATCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGAtttt                                                                                                                                      <  7:98267/149‑1 (MQ=255)
                aCGGTCATCTGCACCAGCCCGAGGCCGATGATGATCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGa                                                                                                                                    <  7:462951/149‑1 (MQ=255)
                  ggTCATCTGCACCAGCCCGAGGCCGATGATGATCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGata                                                                                                                                  <  7:21126/149‑1 (MQ=255)
                    tCATCTGCACCAGCCCGAGGCCGATGATGATCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAATGATTTTGATATc                                                                                                                                <  5:181400/149‑1 (MQ=255)
                    tCATCTGCACCAGCCCGAGGCCGATGATGATCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATc                                                                                                                                <  8:146168/149‑1 (MQ=255)
                      aTCTGCACCAGCCCGAGGCCGATGATGATCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGa                                                                                                                              <  5:116133/149‑1 (MQ=255)
                              cAGCCCGAGGCCGATGATGATCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACg                                                                                                                      >  3:171822/1‑149 (MQ=255)
                               aGCCCGAGGCCGATGATGATCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGc                                                                                                                     <  2:259160/149‑1 (MQ=255)
                                      ggCCGATGATGATCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTc                                                                                                              >  3:373110/1‑149 (MQ=255)
                                        ccGATGATGATCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTcgc                                                                                                            <  2:182027/149‑1 (MQ=255)
                                          gatgatgatCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGa                                                                                                                        <  1:9423/135‑1 (MQ=255)
                                          gatgatgatCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGa                                                                                                                        >  2:9423/1‑135 (MQ=255)
                                           atgatgatCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGa                                                                                                         >  6:389736/1‑149 (MQ=255)
                                            tgatgatCACCGTGGAAATCGCCGACCACTTCGCGCCGCTGATGGGTTTTTCACGTAAGCTACTATAAATAGTCCGCTTTGTTAACGAAACCTTTGACCACCGTCAGGAAAACGATTTTGATCTCGAACCAGACGCTCCATTCGCGGAt                                                                                                        <  5:76512/149‑1 (MQ=255)
                                              atgatCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGt                                                                                                      <  5:37410/149‑1 (MQ=255)
                                               tgatCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGCCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTa                                                                                                     >  6:160657/1‑149 (MQ=255)
                                               tgatCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGCCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTa                                                                                                     >  6:167416/1‑149 (MQ=255)
                                                gatCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTAc                                                                                                    >  6:312712/1‑149 (MQ=255)
                                                  tCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGatat                                                                                                                                 <  2:284663/118‑1 (MQ=255)
                                                  tCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCAAGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGatat                                                                                                                                 >  1:284663/1‑118 (MQ=255)
                                                    aCCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGCCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCa                                                                                                 <  5:167416/148‑1 (MQ=255)
                                                    aCCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGCCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCa                                                                                                 <  5:160657/148‑1 (MQ=255)
                                                            aaTCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAAc                                                                                        >  2:179679/1‑149 (MQ=255)
                                                             aTCGACGACCACTTCGAGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACt                                                                                       <  5:439705/149‑1 (MQ=255)
                                                                 ccGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGAc                                                                                   >  6:12799/1‑149 (MQ=255)
                                                                 ccGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGAc                                                                                   >  7:32175/1‑149 (MQ=255)
                                                                   gACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGAt                                                                                                        <  4:404067/126‑1 (MQ=255)
                                                                   gACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGAt                                                                                                        >  3:404067/1‑126 (MQ=255)
                                                                      cACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGtt                                                                              >  8:155849/1‑149 (MQ=255)
                                                                         ttCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGttttt                                                                           >  8:247318/1‑149 (MQ=255)
                                                                           cgcgCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTc                                                                          <  3:18812/148‑1 (MQ=255)
                                                                             cgccgcTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTGAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCAt                                                                       <  5:173207/149‑1 (MQ=255)
                                                                              gccgcTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCAtt                                                                      <  8:32175/149‑1 (MQ=255)
                                                                                 gcTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTtc                                                                   <  4:124924/149‑1 (MQ=255)
                                                                                    gATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTtctc                                                                 >  1:414117/1‑148 (MQ=255)
                                                                                     aTGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTcc                                                                <  5:312712/148‑1 (MQ=255)
                                                                                        gTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTCAACACCGTCACGAAAACGATTATGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGc                                                             <  8:336258/148‑1 (MQ=255)
                                                                                               cACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGt                                                     >  5:99579/1‑149 (MQ=255)
                                                                                                aCGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGATCCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGtt                                                    >  6:113129/1‑149 (MQ=255)
                                                                                                 cGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGttt                                                   >  8:367025/1‑149 (MQ=255)
                                                                                                  gTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTc                                                  <  2:414117/149‑1 (MQ=255)
                                                                                                  gTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTc                                                  <  7:155849/149‑1 (MQ=255)
                                                                                                          atatCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCa                                                               <  6:188696/128‑1 (MQ=255)
                                                                                                          atatCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCa                                                               >  5:188696/1‑128 (MQ=255)
                                                                                                            atCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATAGAACCAGACGCTCCATTCGCGGATGTACTCAGGGTCGAACTCGACGCGTTTTTCCATTTTCTCCATCGTGTCGGTTTCGCCGCGCCAg                                        >  7:469781/1‑149 (MQ=255)
                                                                                                                atatGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGt                                    <  5:12799/149‑1 (MQ=255)
                                                                                                                atatGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGt                                    <  8:295359/149‑1 (MQ=255)
                                                                                                                atatGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGt                                    >  2:95527/1‑149 (MQ=255)
                                                                                                                ataaGCCGCTTTGATAACGAAACCTTTCAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGGCGGTTTCGCCGCGCCAGCCGt                                    <  7:203478/149‑1 (MQ=255)
                                                                                                                  atGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGtt                                   >  5:154454/1‑148 (MQ=255)
                                                                                                                   tGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGt                                    <  4:346469/146‑1 (MQ=255)
                                                                                                                   tGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGt                                    >  3:346469/1‑146 (MQ=255)
                                                                                                                    gCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAAt                                >  3:273755/1‑149 (MQ=255)
                                                                                                                    gCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAAt                                >  1:254557/1‑149 (MQ=255)
                                                                                                                        cTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTgc                            <  1:179679/149‑1 (MQ=255)
                                                                                                                           tGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTgcgc                          <  2:254557/148‑1 (MQ=255)
                                                                                                                             ttAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGcc                         >  7:133848/1‑147 (MQ=255)
                                                                                                                                  gAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCgg                  >  8:107084/1‑149 (MQ=255)
                                                                                                                                   aaaCCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTc                                                  >  6:326721/1‑116 (MQ=255)
                                                                                                                                   aaaCCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTc                                                  <  5:326721/116‑1 (MQ=255)
                                                                                                                                     aCCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTaa               >  4:337804/1‑149 (MQ=255)
                                                                                                                                     aCCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTaa               >  2:250396/1‑149 (MQ=255)
                                                                                                                                      ccTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAAt              <  6:99579/149‑1 (MQ=255)
                                                                                                                                        tttGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCt                              <  4:236607/131‑1 (MQ=255)
                                                                                                                                        tttGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCt                              >  3:236607/1‑131 (MQ=255)
                                                                                                                                            aaCACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCg         <  5:113129/148‑1 (MQ=255)
                                                                                                                                                 cGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCa   <  3:337804/149‑1 (MQ=255)
                                                                                                                                                  gTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCAc  >  7:381537/1‑149 (MQ=255)
                                                                                                                                                  gTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCAc  >  5:402890/1‑149 (MQ=255)
                                                                                                                                                  gTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCAc  <  8:381537/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GATAATCCGCGCCAGCACGGTCATCTGCACCAGCCCGAGGCCGATGATGATCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCAC  >  NC_000913/2120051‑2120345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: