Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,236,672 T→C 6.0% intergenic (+172/+71) preA → / ← mglC dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C/methyl‑galactoside transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,236,6720TC 6.0% 261.5 / 5.1 85intergenic (+172/+71)preA/mglCdihydropyrimidine dehydrogenase, NADH‑dependent, subunit C/methyl‑galactoside transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (40/39);  new base C (3/2);  total (44/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

GTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGATAATAATCGCCCCTTTGATGATGTACTGC  >  NC_000913/2236525‑2236818
                                                                                                                                                   |                                                                                                                                                  
gTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCATCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCATTa                                                                                                                                                   >  8:163851/1‑149 (MQ=255)
   ccTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATc                                                                                                                                                 <  8:273412/148‑1 (MQ=255)
    cTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCTCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                                <  4:84802/148‑1 (MQ=255)
       tCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                            >  1:246628/1‑149 (MQ=255)
       tCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                            <  3:409918/149‑1 (MQ=255)
         gCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTa                                                                                                                                          <  3:232825/149‑1 (MQ=255)
         gCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTa                                                                                                                                          >  1:353670/1‑149 (MQ=255)
              aTGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACaaaa                                                                                                                                     <  2:353670/149‑1 (MQ=255)
                   gCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACcaca                                                                                                                                <  4:363686/149‑1 (MQ=255)
                           gCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCat                                                                                                                        >  5:260300/1‑149 (MQ=255)
                           gCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCat                                                                                                                        >  5:396521/1‑149 (MQ=255)
                           gCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCat                                                                                                                        >  7:343883/1‑149 (MQ=255)
                             cTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCatga                                                                                                                      >  7:1259/1‑149 (MQ=255)
                                aTCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCatgat                                                                                                                     <  3:872/147‑1 (MQ=255)
                                 tCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATAAGGCGTTCACGCCGCATCCGGcaaca                                                                                                                                         >  3:163444/1‑122 (MQ=38)
                                 tCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATAAGGCGTTCACGCCGCATCCGGcaaca                                                                                                                                         <  4:163444/126‑5 (MQ=38)
                                   cGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTAt                                                                                                                >  6:372850/1‑149 (MQ=255)
                                   cGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTAt                                                                                                                <  4:131358/149‑1 (MQ=255)
                                    ggCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTAtg                                                                                                               >  4:179365/1‑149 (MQ=255)
                                     gCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTAtgt                                                                                                              >  4:155333/1‑149 (MQ=255)
                                     gCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTAtgt                                                                                                              >  8:364628/1‑149 (MQ=255)
                                        tACAAAACCATACAAATCAGTATGTTGAGCTTATTGTATGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTACTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCg                                                                                                           >  3:208470/1‑149 (MQ=255)
                                           aaaaCCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGttt                                                                                                        <  5:413711/149‑1 (MQ=255)
                                           aaaaCCAAACAAAACAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTTCATCAGGCATTTTGATCCTGTTGACGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATAAAGATTTTATGTGCGtg                                                                                                         >  1:274030/1‑147 (MQ=37)
                                                 aTACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCatgatg                                                                                                                    >  2:24104/1‑131 (MQ=255)
                                                 aTACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCatgatg                                                                                                                    <  1:24104/131‑1 (MQ=255)
                                                    cAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGaa                                                                                               <  7:127253/149‑1 (MQ=255)
                                                     aaaTCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAAt                                                                                              >  1:100050/1‑149 (MQ=255)
                                                     aaaTCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAAt                                                                                              <  3:362619/149‑1 (MQ=255)
                                                       aTCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGc                                                                                            >  3:386694/1‑149 (MQ=255)
                                                          aGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTTGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGa                                                                                         <  6:260300/149‑1 (MQ=255)
                                                          aGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGa                                                                                         <  6:396521/149‑1 (MQ=255)
                                                                ttGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACcaca                                                                                    <  4:285740/148‑1 (MQ=255)
                                                                  gAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCTGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACAtt                                                                                  <  2:388356/148‑1 (MQ=255)
                                                                    gCTTATTGTAGGCAGGATAAAACGCTGCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTTTCCGGCCTACAAAACCACACAGATCTTGATTTTATGTGCGTTTATGACCGATTGCGGACCACATTcac                                                                               <  2:274030/149‑1 (MQ=38)
                                                                     cTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTcac                                                                               <  6:402966/148‑1 (MQ=255)
                                                                           gTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCAttt                                                                        >  2:136233/1‑149 (MQ=255)
                                                                               gCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTa                                                                    >  4:42440/1‑149 (MQ=255)
                                                                               gCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTa                                                                    >  6:112899/1‑149 (MQ=255)
                                                                                    aTAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTAcgcgc                                                               <  3:179020/149‑1 (MQ=255)
                                                                                     tAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTca                                                                                <  2:365130/131‑1 (MQ=255)
                                                                                     tAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTca                                                                                >  1:365130/1‑131 (MQ=255)
                                                                                       aaaCGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTa                                                            >  5:253871/1‑149 (MQ=255)
                                                                                        aaCGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTAt                                                           >  6:71939/1‑149 (MQ=255)
                                                                                        aaCGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTAt                                                           >  8:372771/1‑149 (MQ=255)
                                                                                         aCGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTAtt                                                          <  3:155333/149‑1 (MQ=255)
                                                                                          cgcgCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTAttt                                                         <  2:246628/149‑1 (MQ=255)
                                                                                             gcCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAg                                                      >  7:188032/1‑149 (MQ=255)
                                                                                               cAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGt                                                     >  2:226073/1‑148 (MQ=255)
                                                                                               cAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGt                                                     >  3:55602/1‑148 (MQ=255)
                                                                                                aGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCgg                                                                                          <  7:99492/110‑1 (MQ=255)
                                                                                                aGCGTTGCATCCGGCATTTGGCTACTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACACATCATGATGTTATGTGCGTTTATGACATAATGCgg                                                                                          >  8:99492/1‑110 (MQ=25)
                                                                                                   gTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGCACCACATTCACATCATTTCTAACGCGCGAATTTCAGTGAATc                                                >  2:348568/1‑149 (MQ=255)
                                                                                                       cATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAgc                                            <  8:451232/149‑1 (MQ=255)
                                                                                                       cATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGACTACAAAACCAAAAATATCATGATGTTATTTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAgc                                            >  4:59105/1‑149 (MQ=39)
                                                                                                         tCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAgcgc                                          >  5:253587/1‑149 (MQ=255)
                                                                                                             gCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTAtgt                                                                                                              >  3:28585/1‑77 (MQ=255)
                                                                                                             gCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTAtgt                                                                                                              <  4:28585/77‑1 (MQ=255)
                                                                                                             gCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTAATCCGGCCTACAAAACCACACAGATCATGAAGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACg                                      >  6:33160/1‑149 (MQ=255)
                                                                                                               aTTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACATATCATGATGTTATGTTCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGc                                    <  1:381667/149‑1 (MQ=255)
                                                                                                                 ttGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCg                                   >  3:291777/1‑148 (MQ=255)
                                                                                                                   ggCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGaa                                 <  7:364628/148‑1 (MQ=255)
                                                                                                                   ggCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGaa                                 <  3:42440/148‑1 (MQ=255)
                                                                                                                    gCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGa                               <  7:372771/149‑1 (MQ=255)
                                                                                                                      tCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGat                              >  1:291339/1‑148 (MQ=255)
                                                                                                                        cTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGataa                            >  1:74436/1‑148 (MQ=255)
                                                                                                                          taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGata                             >  2:456912/4‑145 (MQ=255)
                                                                                                                          taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGata                             <  1:456912/142‑1 (MQ=255)
                                                                                                                            ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGATAATAATc                       >  2:172228/2‑149 (MQ=255)
                                                                                                                             gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGATAATAATc                       <  4:279955/148‑1 (MQ=255)
                                                                                                                                     cggcgTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGATAATAATCGCCCCTTtg              <  8:1259/149‑1 (MQ=255)
                                                                                                                                     cggcgTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGATAATAATCGCCCCTTtg              <  2:100050/149‑1 (MQ=255)
                                                                                                                                      ggcgTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGCATCCAGCGCTACGGCGAAGATAATAATCGCCCCTTtg              >  8:452692/1‑148 (MQ=255)
                                                                                                                                      ggcgTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGATAATAATCGCCCCTTtga             >  1:195708/1‑149 (MQ=255)
                                                                                                                                      ggcgTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCCAAGATAATAATCGCCCCTTtga             <  1:226073/149‑1 (MQ=255)
                                                                                                                                       gcgTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGATAATAATCGCCCCTTtgat            <  6:253587/149‑1 (MQ=255)
                                                                                                                                        cgTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGATAATAATCGCCCCTTtgatg           <  5:112899/149‑1 (MQ=255)
                                                                                                                                          tGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGATAATAATCGCCCCTTTgatgat         <  4:291777/149‑1 (MQ=255)
                                                                                                                                          tGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGATAATAATCGCCCCTTTgatgat         <  6:261689/149‑1 (MQ=255)
                                                                                                                                          tGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGATAATAATCGCCCCTTTgatgat         <  5:71939/149‑1 (MQ=255)
                                                                                                                                               gCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGATAATAATCGCCCCTTTGATGATGTACt    >  2:141099/1‑149 (MQ=255)
                                                                                                                                               gCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGATAATAATCGCCCCTTTGATGATGTACt    <  3:179365/149‑1 (MQ=255)
                                                                                                                                                ccTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGATAATAATCGCCCCTTTGATGATGTACTg   >  1:369421/1‑149 (MQ=255)
                                                                                                                                                 cTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGATAATAATCGCCCCTTTGATGATGTACTGc  >  8:345501/1‑149 (MQ=255)
                                                                                                                                                 cTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACAACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGATAATAATCGCCCCTTTGATGATGTACTGc  >  6:165451/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTTCCTGTCGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAACCATACAAATCAGTATGTTGAGCTTATTGTAGGCCGGATAAAACGCGCCAGCGTTGCATCCGGCATTTGGCTCCTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCACACAGATCATGATGTTATGTGCGTTTATGACCGAATGCGGACCACATTCACATCATTTCTTACGCGCGTATTTCAGTGAATCCAGCGCTACGGCGAAGATAATAATCGCCCCTTTGATGATGTACTGC  >  NC_000913/2236525‑2236818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: