Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,394,036 G→T 5.6% T337T (ACC→ACA nuoL ← NADH:ubiquinone oxidoreductase, membrane subunit L

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,394,0360GT 5.6% 234.5 / 2.4 71T337T (ACC→ACAnuoLNADH:ubiquinone oxidoreductase, membrane subunit L
Reads supporting (aligned to +/- strand):  ref base G (32/35);  new base T (2/2);  total (34/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

ACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCCAGCAGCAGCGTAACCG  >  NC_000913/2393888‑2394184
                                                                                                                                                    |                                                                                                                                                    
aCAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTgg                                                                                                                                                      <  4:423168/149‑1 (MQ=255)
  aGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGt                                                                                                                                                    <  4:28123/149‑1 (MQ=255)
    tgctgcGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTATAAAGCACGCGTGGGtca                                                                                                                                                  >  1:342241/1‑149 (MQ=255)
         cgccgcCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCaa                                                                                                                                              <  3:346282/148‑1 (MQ=255)
          gccgccCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGt                                                                                                                                            <  5:324702/149‑1 (MQ=255)
                  ccaGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATc                                                                                                                                    >  5:406740/1‑149 (MQ=255)
                    aGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGACCCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATcgc                                                                                                                                  >  8:137815/1‑149 (MQ=255)
                     ggAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCGATCTGGAAGATGTTCTGTTCGTGATGGCAGGGCAGAGTGACGGAACCGGATGCCAGGAACAGCAGCGCGTTAAAGAACGCGTGGGTCATCAAGTGGAAAATcgcc                                                                                                                                 >  6:318282/1‑149 (MQ=255)
                     ggAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATcgcc                                                                                                                                 <  7:140247/149‑1 (MQ=255)
                           agagATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGt                                                                                                                                            <  3:222291/132‑1 (MQ=255)
                           agagATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGt                                                                                                                                            >  4:222291/1‑132 (MQ=255)
                           agagATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATcc                                                                                                                           >  7:50253/1‑149 (MQ=255)
                             agaTAAACCAGCAGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCa                                                                                                                         <  3:301760/149‑1 (MQ=255)
                               aTAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATg                                                                                                                       >  2:258998/1‑149 (MQ=255)
                                      aGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCAc                                                                                                                <  8:54193/149‑1 (MQ=255)
                                           aaTAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTgg                                                                                                                                                      >  7:201333/1‑106 (MQ=255)
                                           aaTAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTgg                                                                                                                                                      <  8:201333/106‑1 (MQ=255)
                                                aTTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAgcgc                                                                                                       >  6:257154/1‑148 (MQ=255)
                                                 tttACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGa                                                                                                     <  6:406740/149‑1 (MQ=255)
                                                 tttACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGa                                                                                                     <  1:258998/149‑1 (MQ=255)
                                                 tttACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGa                                                                                                     <  7:417584/149‑1 (MQ=255)
                                                   tACGCAGACCGCCCATCTTGAAGATGTTCTGTGCGTGAGGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAg                                                                                                    <  2:368685/148‑1 (MQ=255)
                                                     cGCTGACCGCCCATCTTGAAGATGTTGTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGCACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGa                                                                                                  <  5:94380/148‑1 (MQ=255)
                                                     cGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGaa                                                                                                 <  6:143723/149‑1 (MQ=255)
                                                          aCCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGt                                                                                            <  2:231588/149‑1 (MQ=255)
                                                            cGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAg                                                                                          >  7:436927/1‑149 (MQ=255)
                                                                cATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCaa                                                                                      >  6:384197/1‑149 (MQ=255)
                                                                 aTCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAg                                                                                                    >  5:240660/1‑134 (MQ=255)
                                                                 aTCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAg                                                                                                    <  6:240660/134‑1 (MQ=255)
                                                                  tCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCATCGCATTAAAGACCGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATc                                                                                    >  6:335260/1‑149 (MQ=255)
                                                                   cTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCt                                                                                   >  1:466252/1‑149 (MQ=255)
                                                                      gAAGATGTTCTGTTCGTGATGGCAGTCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGTGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCaa                                                                                      >  5:240551/1‑143 (MQ=255)
                                                                      gAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGTGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCaa                                                                                      <  6:240551/143‑1 (MQ=255)
                                                                      gAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTgg                                                                                 <  8:50253/148‑1 (MQ=255)
                                                                      gAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGc                                                                                >  5:326713/1‑149 (MQ=255)
                                                                      gAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGc                                                                                >  2:179789/1‑149 (MQ=255)
                                                                      gAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGc                                                                                >  6:456607/1‑149 (MQ=255)
                                                                            gttctgttcGTGATGGCAGGCCAGAATGACGTAACCGGATGCCAGGAAGAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGGCGGATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATg                                                                           <  8:111520/148‑1 (MQ=255)
                                                                              tctgttcGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATgg                                                                          >  2:166144/1‑147 (MQ=255)
                                                                                  ttcGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTaga                                                                      <  5:384197/147‑1 (MQ=255)
                                                                                  ttcGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTaga                                                                      <  5:257154/147‑1 (MQ=255)
                                                                                  ttcGTGATGGCAGGCCAGAAGGACGGAACCGGAGGCCAGGAACAGCAGCGCTTTAAAGACCGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACTTGTAGCCAATCTGGCTCATGGTaga                                                                      <  4:334065/147‑1 (MQ=255)
                                                                                     gTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAg                                                                 >  8:221993/1‑149 (MQ=255)
                                                                                      tGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGcc                                                                                                             <  7:82939/104‑1 (MQ=255)
                                                                                      tGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGc                                                                <  6:326713/149‑1 (MQ=255)
                                                                                      tGATGGCAGGCCAGAATGACGGAACCGGATCCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGcc                                                                                                             >  8:82939/1‑104 (MQ=255)
                                                                                                ccAGAATGACGGAACCGGATGCCAGGAACAGGAGCGCTTTAAAGATCGGGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATACCTGCACGCGAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGt                                                      <  7:137815/149‑1 (MQ=255)
                                                                                                  aGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGttt                                                    <  1:166144/149‑1 (MQ=255)
                                                                                                       gACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTa                                                                        >  5:291253/1‑124 (MQ=255)
                                                                                                       gACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTa                                                                        <  6:291253/124‑1 (MQ=255)
                                                                                                         cGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCg                                             <  4:174754/149‑1 (MQ=255)
                                                                                                         cGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCg                                             >  7:351290/1‑149 (MQ=255)
                                                                                                          ggAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTa                                                                        <  1:122973/121‑1 (MQ=255)
                                                                                                          ggAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTa                                                                        >  2:122973/1‑121 (MQ=255)
                                                                                                           gAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGt                                           <  5:318282/149‑1 (MQ=255)
                                                                                                             aCCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCt                                         <  1:179789/149‑1 (MQ=255)
                                                                                                                 gATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTAc                                     >  8:108742/1‑149 (MQ=255)
                                                                                                                      cAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTaga                                                                      <  8:252657/111‑1 (MQ=255)
                                                                                                                      cAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTaga                                                                      >  7:252657/1‑111 (MQ=255)
                                                                                                                       aGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACa                                                                                               <  8:73329/85‑1 (MQ=255)
                                                                                                                       aGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACa                                                                                               >  7:73329/1‑85 (MQ=255)
                                                                                                                               cagcGCTTTAAAGAACGCGTGTGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAAc                       <  1:223080/149‑1 (MQ=255)
                                                                                                                               cagcGCTTTAAAGAACGCGTGTGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAAc                       >  2:223080/1‑149 (MQ=255)
                                                                                                                                 gcgcTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCg                     >  6:37815/1‑149 (MQ=255)
                                                                                                                                    cTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGc                   >  7:252298/1‑148 (MQ=255)
                                                                                                                                     tttAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGc                   >  8:308439/1‑147 (MQ=255)
                                                                                                                                          aGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGt                                               <  4:147262/114‑1 (MQ=255)
                                                                                                                                          aGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGt                                               >  3:147262/1‑114 (MQ=255)
                                                                                                                                           gAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAAc                                                                                                >  5:74431/1‑64 (MQ=255)
                                                                                                                                           gAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAAc                                                                                                <  6:74431/64‑1 (MQ=255)
                                                                                                                                           gAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCcagcagca           <  8:351290/149‑1 (MQ=255)
                                                                                                                                            aaCGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCcagcagcag          >  6:130891/1‑149 (MQ=255)
                                                                                                                                             actcgTGGGTCATCAAGTGGAAAATCGCCTCATCCCAAGCCTGCACGCCAAGCGCGAGGAACATGTAGACAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGt                                           >  2:136741/4‑115 (MQ=255)
                                                                                                                                             aCGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGAGAGGAACATGTATCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATTTCGGt                                           <  1:136741/115‑1 (MQ=255)
                                                                                                                                                    ggTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCCAGCAGCAGCGTAACCg  >  8:185022/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACAGTGCTGCGCCGCCCACCAGGAAGCAGAGATAAACCAGCGGAATAGATTTACGCAGACCGCCCATCTTGAAGATGTTCTGTTCGTGATGGCAGGCCAGAATGACGGAACCGGATGCCAGGAACAGCAGCGCTTTAAAGAACGCGTGGGTCATCAAGTGGAAAATCGCCGCATCCCATGCCTGCACGCCAAGCGCGAGGAACATGTAGCCAATCTGGCTCATGGTAGAGTAAGCGAGAACACGTTTGATGTCGGTCTGTACCAGCGCGGCAAAACCGGCCAGCAGCAGCGTAACCG  >  NC_000913/2393888‑2394184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: