Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,411,925 A→T 5.3% F55L (TTT→TTA) ‡ yfbT ← sugar phosphatas

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,411,9250AT 5.3% 299.3 / 5.6 94F55L (TTT→TTA) ‡yfbTsugar phosphatas
Reads supporting (aligned to +/- strand):  ref base A (41/48);  new base T (2/3);  total (43/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

AATGGCCCACGGAATACCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAG  >  NC_000913/2411778‑2412071
                                                                                                                                                   |                                                                                                                                                  
aaTGGCCCACGGAATACCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGTTGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGAGTTGCCCGCCATa                                                                                                                                                    >  3:276647/1‑148 (MQ=255)
     cccACGGAATACCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATgg                                                                                                                                              <  3:420535/149‑1 (MQ=255)
      ccACGGAATACCTGCTTTATTCAAATGACTCAGTAAGGCGATTGCCCACGGAAGCGCTGTAATACCTTCGGTTTCAGTGGACTCTATGTGCTCCAGACGCGTAAACTCGGCTGCAATATCAGCCTCGTATTTGCCCGCCATaaaaag                                                                                                                                               >  8:367390/1‑145 (MQ=255)
      caaCGGAATACCTGCTATATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGgc                                                                                                                                             >  2:270652/3‑149 (MQ=255)
       cACGGAATACCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGgcg                                                                                                                                            >  1:205044/1‑149 (MQ=255)
         cGGAATACCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATTTTATGgcggg                                                                                                                                          <  7:256499/149‑3 (MQ=255)
           gAATACCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCaga                                                                                                                                        <  8:33430/149‑1 (MQ=255)
           gAATACCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCaga                                                                                                                                        <  7:367390/149‑1 (MQ=255)
              tACCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCagagag                                                                                                                                     <  3:363804/149‑1 (MQ=255)
              tACCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCagagag                                                                                                                                     <  6:189259/149‑1 (MQ=255)
              tACCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCagagag                                                                                                                                     <  5:441141/149‑1 (MQ=255)
                ccTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCGGCCGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGt                                                                                                                                   >  6:219662/1‑149 (MQ=255)
                ccTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAgg                                                                                                                                    <  6:202014/148‑1 (MQ=255)
                ccTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGt                                                                                                                                   >  5:278722/1‑149 (MQ=255)
                         ttCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTg                                                                                                                          >  4:203715/1‑149 (MQ=255)
                         ttCAAATGACTGAGTAAGGCGATTGCCACCGGAAGCGCGGTAATACCTTCGGTTTCCTTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGCCGCAGAGAGGTGATCGCCTg                                                                                                                          >  3:269723/1‑149 (MQ=255)
                            aaaTGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCaga                                                                                                                                        >  4:418589/1‑132 (MQ=255)
                            aaaTGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCaga                                                                                                                                        <  3:418589/132‑1 (MQ=255)
                            aaaTGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGtt                                                                                                                        >  8:171205/1‑148 (MQ=255)
                            aaaTGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGtt                                                                                                                        >  3:98402/1‑148 (MQ=255)
                            aaaTGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGtt                                                                                                                        >  6:241443/1‑148 (MQ=255)
                            aaaTGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGtt                                                                                                                        >  7:113305/1‑148 (MQ=255)
                            aaaTGACTGAGTAAGGCGATTGCCCCCGGAAGAGCGGTAATACCTTCTGATTCAGTGGCCTCTATGTGCTCCAGACGCGTAAACTCTGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGACTGtt                                                                                                                        >  1:127692/1‑148 (MQ=255)
                             aaTGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTa                                                                                                                      >  7:320666/1‑149 (MQ=255)
                               tGACTGAGTACGGCGATTGCCCCCTGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTAcc                                                                                                                    <  4:269723/149‑1 (MQ=255)
                               tGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTAcc                                                                                                                    <  3:366293/149‑1 (MQ=255)
                                 aCTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGt                                                                                                                  <  5:300010/149‑1 (MQ=255)
                                   tGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCa                                                                                                                                          >  2:148688/1‑123 (MQ=255)
                                   tGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCa                                                                                                                                          <  1:148688/123‑1 (MQ=255)
                                     aGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGtgaat                                                                                                              <  2:121509/149‑1 (MQ=255)
                                     aGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGtgaa                                                                                                               <  1:270652/148‑1 (MQ=255)
                                         aGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGaaa                                                                                                          <  4:376422/149‑1 (MQ=255)
                                         aGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGaaa                                                                                                          <  7:116936/149‑1 (MQ=255)
                                         aGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGaaa                                                                                                          <  1:167088/149‑1 (MQ=255)
                                         aGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCACAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGaaa                                                                                                          <  5:218890/149‑1 (MQ=255)
                                           gCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGc                                                                                                        <  8:113305/149‑1 (MQ=255)
                                             gATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGAAAACTCGGCGGCAATATAAGCCTCGGATTTGCCCGACATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGc                                                                                                        >  1:83702/1‑147 (MQ=255)
                                                 gCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATAAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCa                                                                                                   <  5:75925/148‑1 (MQ=255)
                                                  cccccGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGc                                                                                                        >  1:289368/1‑142 (MQ=255)
                                                  cccccGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGc                                                                                                        <  2:289368/142‑1 (MQ=255)
                                                   ccccGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATTTTATGgcgg                                                                                                                                           <  3:212995/106‑2 (MQ=255)
                                                   ccccGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATTTTATGgcgg                                                                                                                                           >  4:212995/1‑105 (MQ=255)
                                                         aaGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCg                                                                                          <  1:299925/149‑1 (MQ=255)
                                                          aGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCgg                                                                                         >  8:392046/1‑149 (MQ=255)
                                                          aGCGCGGTAATACCTTCGGTTTCCGTGGACTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTAAAGTGAATGAAAGCCAGCACCTCTTCCgg                                                                                         >  3:338663/1‑149 (MQ=255)
                                                           gcgcGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCCGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCCCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGgc                                                                                        <  5:219662/149‑1 (MQ=255)
                                                             gcgGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATTTTATGgcgggc                                                                                                                                         >  6:384847/1‑95 (MQ=255)
                                                             gcgGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATTTTATGgcgggc                                                                                                                                         <  5:384847/98‑4 (MQ=255)
                                                             gcgGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGtgaat                                                                                                              <  3:417705/125‑1 (MQ=255)
                                                             gcgGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGtgaat                                                                                                              >  4:417705/1‑125 (MQ=255)
                                                                 tAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAAc                                                                                  <  4:98402/149‑1 (MQ=255)
                                                                             tttCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTg                                                                      >  4:12506/1‑149 (MQ=255)
                                                                               tCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCACGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGtgaatgaat                                                                                                          >  2:205911/1‑110 (MQ=255)
                                                                                  gTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGcc                                                                   >  5:434759/1‑147 (MQ=255)
                                                                                   tGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCt                                                                                     >  2:232530/1‑128 (MQ=255)
                                                                                   tGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCt                                                                                     <  1:232530/128‑1 (MQ=255)
                                                                                   tGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAg                                                                <  5:241443/149‑1 (MQ=255)
                                                                                       cTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGaaa                                                                                                          >  7:6839/1‑103 (MQ=255)
                                                                                       cTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGaaa                                                                                                          <  8:6839/103‑1 (MQ=255)
                                                                                          gATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGtgaat                                                                                                              >  2:35640/1‑96 (MQ=255)
                                                                                          gATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGtgaat                                                                                                              <  1:35640/96‑1 (MQ=255)
                                                                                          gATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACct                                                                                                >  1:395398/1‑110 (MQ=255)
                                                                                          gATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACct                                                                                                <  2:395398/110‑1 (MQ=255)
                                                                                            tgtgCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCAc                                                       <  4:338663/149‑1 (MQ=255)
                                                                                                cTCCAGACGCGTAAACTCGGCGGCAATATGACCCTCGGAGTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTTCCGTGATTGAAAGCCAGCACCTCTTCCCGCGCTAACCCATGACTTGTGGCCCAGTTGCTCCACGccc                                                   <  2:127692/149‑1 (MQ=255)
                                                                                                cTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGccc                                                   <  8:320666/149‑1 (MQ=255)
                                                                                                    aGATGCGTAAACTCGGCTGCTATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGGGATCGCCTGTTTACGGTGACTGATAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCAGTCCCGTTc                                               <  2:83702/149‑1 (MQ=255)
                                                                                                         cgTAAACTCGGCGGCAATATCAGACTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGTCGCTAACCCATGACTTCTGGCCCAGTTGATCCACGCCCGTTCTACCg                                          >  4:299603/1‑149 (MQ=255)
                                                                                                          gTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCg                                          >  3:267366/1‑148 (MQ=255)
                                                                                                            aaaCTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGaaa                                                                                                          >  2:160800/1‑82 (MQ=255)
                                                                                                            aaaCTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGaaa                                                                                                          <  1:160800/82‑1 (MQ=255)
                                                                                                             aaCTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCa                                                                 <  2:152714/122‑1 (MQ=255)
                                                                                                             aaCTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCa                                                                 <  4:411005/122‑1 (MQ=255)
                                                                                                             aaCTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCa                                                                 >  3:411005/1‑122 (MQ=255)
                                                                                                             aaCTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCa                                                                 >  1:152714/1‑122 (MQ=255)
                                                                                                             aaCTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCgcagg                                      <  6:434759/149‑1 (MQ=255)
                                                                                                              aCTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCgcaggc                                     <  6:278722/149‑1 (MQ=255)
                                                                                                                tCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCgcaggcag                                   >  3:157977/1‑149 (MQ=255)
                                                                                                                          tatCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATccac                          >  8:71716/1‑148 (MQ=255)
                                                                                                                            tCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGt                                                 <  1:28840/123‑1 (MQ=255)
                                                                                                                            tCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGt                                                 >  2:28840/1‑123 (MQ=255)
                                                                                                                            tCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATccacca                        <  7:171205/148‑1 (MQ=255)
                                                                                                                             cAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAg                                                                >  6:255820/1‑107 (MQ=255)
                                                                                                                             cAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAg                                                                <  5:255820/107‑1 (MQ=255)
                                                                                                                                    gtaTTTGCCCGACATAAAATGGCGCAGATAGGTGATCGCCTGTTTACCTTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGGTCCACGCCCGTTCTACCGCAGGCAGGGATTTAACCAGCGTTcc                 <  8:340681/145‑1 (MQ=255)
                                                                                                                                     gATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATc              <  4:267366/149‑1 (MQ=255)
                                                                                                                                           cccGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATc        <  7:392046/149‑1 (MQ=255)
                                                                                                                                           cccGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAACGCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATc        <  2:205044/149‑1 (MQ=255)
                                                                                                                                            ccGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTa                                             >  4:134647/1‑111 (MQ=255)
                                                                                                                                            ccGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTa                                             <  3:134647/111‑1 (MQ=255)
                                                                                                                                            ccGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATccacca                        <  6:314220/132‑1 (MQ=255)
                                                                                                                                            ccGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATccacca                        >  5:314220/1‑132 (MQ=255)
                                                                                                                                            ccGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATc        >  8:162474/1‑148 (MQ=255)
                                                                                                                                              gCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCaaa     >  2:107979/1‑149 (MQ=255)
                                                                                                                                                  tAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGCATCCACCAGCGTTCCATCAAGATCAAACAg  >  3:26995/1‑148 (MQ=255)
                                                                                                                                                  tAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCAATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAg  >  7:219720/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AATGGCCCACGGAATACCTGCTTTATTCAAATGACTGAGTAAGGCGATTGCCCCCGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAG  >  NC_000913/2411778‑2412071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: