Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,613,332 C→T 5.5% P478L (CCG→CTG)  hyfR → hydrogenase‑4 transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,613,3320CT 5.5% 223.8 / 2.5 73P478L (CCG→CTG) hyfRhydrogenase‑4 transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (33/35);  new base T (2/2);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

ATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTT  >  NC_000913/2613185‑2613480
                                                                                                                                                   |                                                                                                                                                    
aTACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCg                                                                                                                                                     <  6:375597/149‑1 (MQ=255)
 tACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCgg                                                                                                                                                    >  1:410921/1‑149 (MQ=255)
  acgcATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGGTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTTAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGt                                                                                                                                                   <  5:235000/146‑1 (MQ=255)
   cccATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGt                                                                                                                                                   <  8:59008/148‑1 (MQ=255)
    ccATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTAGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGTAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTg                                                                                                                                                  >  7:215724/1‑148 (MQ=255)
     cATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGaa                                                                                                                                                >  1:353949/1‑149 (MQ=255)
         gTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTc                                                                                                                                            <  7:398408/149‑1 (MQ=255)
          tGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCa                                                                                                                                           <  7:292354/149‑1 (MQ=255)
            gTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCaga                                                                                                                                         <  2:410921/149‑1 (MQ=255)
            gTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCATATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCaga                                                                                                                                         <  6:248066/149‑1 (MQ=255)
             tCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCagag                                                                                                                                        >  3:307559/1‑149 (MQ=255)
             tCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCagag                                                                                                                                        >  1:195893/1‑149 (MQ=255)
                            cGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCACGGTGAATGTCAGAGTCATTGCCGccacc                                                                                                                          >  6:80284/1‑148 (MQ=255)
                              aTGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAAc                                                                                                                       >  3:135980/1‑149 (MQ=255)
                                 gcggcACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCACGGTGAATGTCAGAGTCATTGCCGCCACCAACCGt                                                                                                                    <  5:80284/149‑1 (MQ=255)
                                    gcACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGGCAGAGTCATTGCCGCCACCAACCGTGAt                                                                                                                 <  1:116693/149‑1 (MQ=255)
                                      aCGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCTGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGAttt                                                                                                               >  3:4733/1‑149 (MQ=255)
                                      aCGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGAttt                                                                                                               >  7:255224/1‑149 (MQ=255)
                                       cGTTGTTTCCCGATGAAATTGGCGCTCTGCCGGTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATCTCAGAGTCATTGCCGCCACCAACCGTGATTtg                                                                                                              <  6:157630/149‑1 (MQ=255)
                                        gttgttTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCTCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCATAGTCAtt                                                                                                                                   >  8:444272/1‑127 (MQ=255)
                                                  gATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATgg                                                                                                    <  6:1042/148‑1 (MQ=255)
                                                  gATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGt                                                                                                   >  3:154137/1‑149 (MQ=255)
                                                  gATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGt                                                                                                   >  3:255727/1‑149 (MQ=255)
                                                  gATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGt                                                                                                   >  2:235149/1‑149 (MQ=255)
                                                   aagAAATTGGCGATCAGCCGTAAGAACTACAGCCTAAACTGCTCCCCGTATTGCAGGAGCCGGAGATTGAGCGGCTCGGCGGGAGTAGAACGATCCCGGTGAAAGTCAGAGTCAGTGCCGCCACCAAACGTGATTTGAGGAAAATTGtt                                                                                                  >  6:341550/3‑149 (MQ=255)
                                                   aTGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTCTTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGtt                                                                                                  <  5:250451/149‑1 (MQ=255)
                                                   aTGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGtt                                                                                                  >  1:21583/1‑149 (MQ=255)
                                                    tGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTg                                                                                                 <  3:422699/149‑1 (MQ=255)
                                                    tGAAATTGGCGATCTGCCGGTAGAACTTCAGCCTAACCTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCAATGCCGCCACCAACCGTGATTTGTGGCAAATGGtt                                                                                                  <  6:342034/148‑1 (MQ=255)
                                                     gAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTg                                                                                                 >  7:446993/1‑148 (MQ=255)
                                                       aaTTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATgg                                                                                                    >  6:242574/1‑143 (MQ=255)
                                                       aaTTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATgg                                                                                                    <  5:242574/143‑1 (MQ=255)
                                                        aTTGGCGATCTGCCGTTAGACCTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCTGGTGAATGTCAGAGTCATTgccgc                                                                                                                              >  3:223243/1‑116 (MQ=255)
                                                        aTTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCTGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGa                                                                                             <  4:4733/149‑1 (MQ=255)
                                                        aTTGGCGATCTGCCGTTAGAACTTAAGACTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCTGGTGAATGTCAGAGTCATTgccgc                                                                                                                              <  4:223243/116‑1 (MQ=255)
                                                         ttGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGAt                                                                                            <  7:135684/149‑1 (MQ=255)
                                                           ggctttCTGCCGTTATAACTTCTGCCTTAAGTGGTGCGCGTATTTTATGAGAGGGAGATTGTTAGTATAGGCGTGATTAGTACGTTCCCTGTGAATGTCAGAGTCATTGGCGCCACGTACCGTGTTTTGTGGCAAATGGTTGAAGATc                                                                                           <  2:21583/143‑1 (MQ=255)
                                                             cGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGc                                                                                         >  3:401828/1‑148 (MQ=255)
                                                               aTCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAg                                                                                      >  7:3515/1‑149 (MQ=255)
                                                                   gCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTc                                                                                  >  1:401941/1‑149 (MQ=255)
                                                                   gCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTc                                                                                  >  5:335097/1‑149 (MQ=255)
                                                                    ccGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTc                                                                                  <  2:401941/148‑1 (MQ=255)
                                                                    ccGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCg                                                                                 <  2:195893/149‑1 (MQ=255)
                                                                      gTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCg                                                                                 >  8:448186/1‑147 (MQ=255)
                                                                         agtaCTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGc                                                                             <  6:335097/145‑1 (MQ=255)
                                                                                 aGCCTAAACTGCTTCGCGTATTGCAGGAGCGGGAGATTGAGCGGCTCGGCGGGAGTAGAACTATCCAGGTGTATTTCAGAGTCATTGCCGCCACCAAGAGTGATTTGTGGCAAATGGTTGAATATCGCCAGTTTCGCAGCTATCTCtt                                                                     <  5:341550/148‑1 (MQ=255)
                                                                                 aGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCttt                                                                    <  4:307559/149‑1 (MQ=255)
                                                                                   ccTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTc                                                                                  <  1:161835/133‑1 (MQ=255)
                                                                                   ccTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTc                                                                                  >  2:161835/1‑133 (MQ=255)
                                                                                   ccTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTa                                                                  >  2:192428/1‑149 (MQ=255)
                                                                                   ccTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTa                                                                  >  1:237368/1‑149 (MQ=255)
                                                                                     tAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCa                                                                               >  2:279468/1‑134 (MQ=255)
                                                                                     tAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCa                                                                               <  1:279468/134‑1 (MQ=255)
                                                                                       aaCTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGc                                                              <  2:353949/149‑1 (MQ=255)
                                                                                        aCTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGcc                                                             <  6:383666/149‑1 (MQ=255)
                                                                                         ctgctgCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCt                                                            <  4:135980/149‑1 (MQ=255)
                                                                                         ctgctgCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCt                                                            <  4:255727/149‑1 (MQ=255)
                                                                                               cgcgTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAAt                                                        <  7:448186/147‑1 (MQ=255)
                                                                                               cgcgTATTGCAGGAGCGGGAGATTGAGCGTCTCGGAGGGAGTAGAACGATCCCTGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAAt                                                        <  8:215724/147‑1 (MQ=255)
                                                                                                    aTTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTcc                                                 >  7:379827/1‑149 (MQ=255)
                                                                                                        cAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACt                                             <  8:255224/149‑1 (MQ=255)
                                                                                                         aGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTg                                            >  7:137761/1‑149 (MQ=255)
                                                                                                         aGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTg                                            <  8:446993/149‑1 (MQ=255)
                                                                                                             gCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAAt                                        <  4:154137/149‑1 (MQ=255)
                                                                                                                 gagaTTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTgcc                                    <  5:42501/149‑1 (MQ=255)
                                                                                                                 gagaTTGAGCGTCTAGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATAGCCGCCACCAACCCTGATTTGTGGCAAATGGTAGAAGATCGCCAGATTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAAATgac                                    >  2:92505/1‑147 (MQ=255)
                                                                                                                         gCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTAc                            <  8:137761/149‑1 (MQ=255)
                                                                                                                            tctcGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTAcgc                          >  5:266359/1‑148 (MQ=255)
                                                                                                                              tcGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGAc                       >  2:462933/1‑149 (MQ=255)
                                                                                                                                ggcggGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGa                        <  1:192428/146‑1 (MQ=255)
                                                                                                                                    ggAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCg                 >  5:143044/1‑149 (MQ=255)
                                                                                                                                        tAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCgg                >  8:297764/1‑146 (MQ=255)
                                                                                                                                               aTCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATccc      >  4:7293/1‑149 (MQ=255)
                                                                                                                                                  ccGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCAGTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCctct   <  4:401828/149‑1 (MQ=255)
                                                                                                                                                   cGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCtt  >  2:441783/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTT  >  NC_000913/2613185‑2613480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: