Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,684,083 C→T 8.1% intergenic (+27/+171) yphH → / ← glyA putative DNA‑binding transcriptional regulator/serine hydroxymethyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,684,0830CT 8.1% 289.0 / 11.2 98intergenic (+27/+171)yphH/glyAputative DNA‑binding transcriptional regulator/serine hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base C (48/42);  new base T (5/3);  total (53/45)
Fisher's exact test for biased strand distribution p-value = 7.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01

Only 100 of 106 total aligned reads displayed.

CGACCGCGACGAAGGACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACAT  >  NC_000913/2683936‑2684231
                                                                                                                                                   |                                                                                                                                                    
cGACCGCGACGAAGGACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTccc                                                                                                                                                      <  8:389176/148‑1 (MQ=255)
cGACCGCGACGAAGGACCGAGCGGGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTccc                                                                                                                                                      <  6:2844/148‑1 (MQ=255)
  aCCGCGACGAAGGACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCgaa                                                                                                                                                   <  6:214350/149‑1 (MQ=255)
   ccGCGACGAAGGACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCgaag                                                                                                                                                  <  4:233402/149‑1 (MQ=255)
    ctcgacgaAGGACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCgaag                                                                                                                                                  <  5:352382/146‑1 (MQ=255)
    cgcgACGAAGGACCGAGCATGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCgaag                                                                                                                                                  >  7:359588/1‑148 (MQ=255)
       gacgaAGGACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCgaag                                                                                                                                                  >  6:352382/1‑145 (MQ=255)
         cgaAGGACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGcc                                                                                                                                            <  2:367842/149‑1 (MQ=255)
          gaAGGACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCTTCGGGAAGCCt                                                                                                                                           <  3:348992/149‑1 (MQ=255)
          gaAGGACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCt                                                                                                                                           >  1:230645/1‑149 (MQ=255)
           aaGGACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCtt                                                                                                                                          <  1:412188/149‑1 (MQ=255)
            aGGACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTtg                                                                                                                                         <  7:289151/149‑1 (MQ=255)
              gACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTtgat                                                                                                                                       <  8:153003/149‑1 (MQ=255)
               ttagAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTtgatg                                                                                                                                      <  3:427147/146‑1 (MQ=255)
                   aGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCa                                                                                                                                  >  3:373675/1‑149 (MQ=255)
                                 gCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTg                                                                                                                    >  1:154865/1‑149 (MQ=255)
                                  cAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGc                                                                                                                   <  5:143129/149‑1 (MQ=255)
                                        ggCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGAt                                                                                                              <  4:338377/148‑1 (MQ=255)
                                           tttGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATgcgc                                                                                                          >  5:334524/1‑149 (MQ=255)
                                           tttGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATgcgc                                                                                                          >  2:145535/1‑149 (MQ=255)
                                           tttGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATgcgc                                                                                                          >  8:272704/1‑149 (MQ=255)
                                                gCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGc                                                                                                     <  4:327246/149‑1 (MQ=255)
                                                    ttAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGCTCATAAACGAAAAATTGCCTGATGCGCTACGCTTAt                                                                                                 >  5:6293/1‑149 (MQ=255)
                                                    ttAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTAt                                                                                                 <  7:272704/149‑1 (MQ=255)
                                                       aGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCa                                                                                               <  1:3109/148‑1 (MQ=255)
                                                       aGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAg                                                                                              >  8:361353/1‑149 (MQ=255)
                                                        gCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAgg                                                                                             >  1:278898/1‑149 (MQ=255)
                                                            cgcACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTa                                                                                         <  4:373675/149‑1 (MQ=255)
                                                             gcACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTa                                                                                         >  8:126942/1‑148 (MQ=255)
                                                              cacaACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTaca                                                                                       <  7:127126/149‑1 (MQ=255)
                                                                      gTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCTGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGAtt                                                                               >  2:346863/1‑149 (MQ=255)
                                                                      gTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTg                                                                                                                >  4:89369/1‑116 (MQ=255)
                                                                      gTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTg                                                                                                                <  3:89369/116‑1 (MQ=255)
                                                                          tGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCaa                                                                           >  3:114848/1‑149 (MQ=255)
                                                                            ggAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAata                                                                         >  5:127687/1‑149 (MQ=255)
                                                                            ggAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTCGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAat                                                                          <  8:336425/148‑1 (MQ=255)
                                                                                ttGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATAtt                                                                     <  1:145535/149‑1 (MQ=255)
                                                                                  ggCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTg                                                                    >  5:380067/1‑148 (MQ=255)
                                                                                      aTTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGgaga                                                                                 <  6:268481/131‑1 (MQ=255)
                                                                                      aTTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGgaga                                                                                 >  5:268481/1‑131 (MQ=255)
                                                                                       tttGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAAttt                                                               <  7:361353/148‑1 (MQ=255)
                                                                                       tttGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTg                                                              >  2:142229/1‑149 (MQ=255)
                                                                                          gTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCa                                                            >  4:396924/1‑148 (MQ=255)
                                                                                          gTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCa                                                            >  3:150614/1‑148 (MQ=255)
                                                                                          gTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCa                                                            >  7:115926/1‑148 (MQ=255)
                                                                                          gTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGCAAGCCTTGATGATCATAAACGAAAAATTGcc                                                                                                                  <  5:452385/94‑1 (MQ=255)
                                                                                          gTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCACGAAGGAAGCCTTGATGATCATAAACGAAAAATTccc                                                                                                                  >  6:452385/1‑94 (MQ=255)
                                                                                           tATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCa                                                            >  7:37744/1‑147 (MQ=255)
                                                                                           tATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCa                                                            >  4:255317/1‑147 (MQ=255)
                                                                                                  aGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTg                                                    <  3:237036/148‑1 (MQ=255)
                                                                                                   ggCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTa                                                  <  3:180455/149‑1 (MQ=255)
                                                                                                   ggCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTa                                                  <  5:281779/149‑1 (MQ=255)
                                                                                                       cAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGcc                                              <  6:6293/149‑1 (MQ=255)
                                                                                                       cAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGcc                                              <  8:37744/149‑1 (MQ=255)
                                                                                                          ttACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCTGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGa                                           <  1:346863/149‑1 (MQ=255)
                                                                                                          ttACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGa                                           >  2:111717/1‑149 (MQ=255)
                                                                                                          ttACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGa                                           >  5:164569/1‑149 (MQ=255)
                                                                                                              gACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGtttt                                                     <  5:90713/135‑1 (MQ=255)
                                                                                                              gACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGtttt                                                     >  6:90713/1‑135 (MQ=255)
                                                                                                              gACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAg                                       >  6:149374/1‑149 (MQ=255)
                                                                                                               aCAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAgg                                      >  1:379745/1‑149 (MQ=255)
                                                                                                                cAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGTAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGACTACACGGAGATTGCAATATATAGAATTTGCAAAGATTTGTAGGCAGGATAAGGc                                     >  4:53211/1‑149 (MQ=38)
                                                                                                                cAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGc                                     <  5:149374/149‑1 (MQ=255)
                                                                                                                cAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGc                                     <  1:111717/149‑1 (MQ=255)
                                                                                                                   aTTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGtt                                  <  2:154865/149‑1 (MQ=255)
                                                                                                                    tttGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATaa                                        <  3:90408/142‑1 (MQ=255)
                                                                                                                    tttGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATaa                                        >  4:90408/1‑142 (MQ=255)
                                                                                                                    tttGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTACAAAGTTTTGTAGGCCGGATAAGGCGtt                                  >  7:266262/1‑148 (MQ=255)
                                                                                                                    tttGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATAATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTc                                 <  6:96458/149‑1 (MQ=255)
                                                                                                                    tttGATGGCGCGATAACGTAGAAAGGCTTCACGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCATACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGtt                                  <  3:53211/148‑1 (MQ=255)
                                                                                                                      tGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCa                                <  8:359588/148‑1 (MQ=255)
                                                                                                                         tGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCAcgcc                            <  2:230645/149‑1 (MQ=255)
                                                                                                                             gcgATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAAttt                                                               <  4:289/110‑1 (MQ=255)
                                                                                                                             gcgATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGc                                     <  7:332721/136‑1 (MQ=255)
                                                                                                                             gcgATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGc                                     >  8:332721/1‑136 (MQ=255)
                                                                                                                             gcgATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCATGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAAttt                                                               >  3:289/1‑110 (MQ=255)
                                                                                                                                   gatcatcaaGGCTTCCTTCGGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                  >  3:159697/8‑149 (MQ=21)
                                                                                                                                   aCGTAGAAATGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCATAGCAGGCCTCCACTTGGATTGCAATATATTTAATTTGCAAAGTTTTGTAGGCCGGATAATGCGTTCACTCCTCATCGGGCa                  >  8:331458/1‑149 (MQ=21)
                                                                                                                                     gTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAgg                                      >  7:314387/1‑127 (MQ=255)
                                                                                                                                     gTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAgg                                      <  8:314387/127‑1 (MQ=255)
                                                                                                                                     gTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAt                 >  8:156411/1‑148 (MQ=255)
                                                                                                                                     gTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAt                 >  8:368151/1‑148 (MQ=255)
                                                                                                                                       atcaaGGCTTCCTTCGGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGtt                                  >  8:154522/4‑129 (MQ=21)
                                                                                                                                       atcaaGGCTTCCTTCGGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGtt                                  <  7:154522/126‑1 (MQ=21)
                                                                                                                                        tcaaGGCTTCCTTCGGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaa              >  2:92425/3‑148 (MQ=255)
                                                                                                                                        tcaaGGCTTCCTTCGGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaa              >  5:99766/3‑148 (MQ=255)
                                                                                                                                         aaaGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaac             >  1:324092/1‑148 (MQ=255)
                                                                                                                                         aaaGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaac             >  3:183989/1‑148 (MQ=255)
                                                                                                                                           aGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGTGATTGCAATATTTTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCTCGACGCATCCTGCATGaacagc          >  4:414288/1‑147 (MQ=25)
                                                                                                                                           aGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaacaac          >  4:420872/1‑149 (MQ=255)
                                                                                                                                           aGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaacaac          <  4:150614/149‑1 (MQ=255)
                                                                                                                                               ttCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGc      >  6:217351/1‑149 (MQ=255)
                                                                                                                                               ttCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGCCATGaacaactat       >  5:314683/1‑145 (MQ=255)
                                                                                                                                                tCCCGAAGGAAGCCTTGATGATCATAAACGCAAAATTGCCTGATGCGATACGCTTATCAGGCCTACCCGTAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCTTTCACGCCGCATCCGGCATGAACAACGAGca     >  7:324846/1‑149 (MQ=21)
                                                                                                                                                 cccGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGc      <  6:241453/147‑1 (MQ=255)
                                                                                                                                                 cccGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTACAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGc      <  8:266262/147‑1 (MQ=255)
                                                                                                                                                  ccGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAgg                                      <  7:410558/114‑1 (MQ=255)
                                                                                                                                                  ccGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAgg                                      >  8:410558/1‑114 (MQ=255)
                                                                                                                                                  ccGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGcaca   >  5:216322/1‑149 (MQ=255)
                                                                                                                                                   cGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACAt  >  2:5205/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGACCGCGACGAAGGACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGATGGCGCGATAACGTAGAAAGGCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACAT  >  NC_000913/2683936‑2684231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: