Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,801,056 A→T 7.3% N31I (AAT→ATT)  nrdI → NrdEF cluster assembly flavodoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,801,0560AT 7.3% 347.6 / 12.4 110N31I (AAT→ATT) nrdINrdEF cluster assembly flavodoxin
Reads supporting (aligned to +/- strand):  ref base A (58/44);  new base T (2/6);  total (60/50)
Fisher's exact test for biased strand distribution p-value = 1.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 114 total aligned reads displayed.

TGGTTTCCGTCCGGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCT  >  NC_000913/2800908‑2801204
                                                                                                                                                    |                                                                                                                                                    
tggtTTCCGTCCGGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCaa                                                                                                                                                      >  5:245793/1‑149 (MQ=255)
  gtTTCCGTCCGGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAAt                                                                                                                                                     >  6:103597/1‑148 (MQ=255)
   tttCCGTCCGGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGa                                                                                                                                                   <  2:155210/149‑1 (MQ=255)
       cgtccgGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCgg                                                                                                                                               <  1:61676/149‑1 (MQ=255)
         tccgGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCATTGAGCggg                                                                                                                                              >  7:154516/1‑148 (MQ=255)
         tccgGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCATTGAGCGGGa                                                                                                                                             <  4:275765/149‑1 (MQ=255)
          ccgGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCATTGAGCGGGat                                                                                                                                            <  4:412861/149‑2 (MQ=255)
           cgGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCATTGAGCGGGat                                                                                                                                            <  6:215001/148‑2 (MQ=255)
           cgGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAAc                                                                                                                                           <  5:103597/149‑1 (MQ=255)
           cgGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAAc                                                                                                                                           <  4:229921/149‑1 (MQ=255)
                aTGATTAACCGTCTGCATACAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGAtt                                                                                                                                      <  4:407995/149‑1 (MQ=255)
                  gATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGAtt                                                                                                                                      >  3:135619/1‑147 (MQ=255)
                   aTTAACCGTCTGCATTCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAg                                                                                                                                   >  6:303029/1‑149 (MQ=255)
                      aaCCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAgg                                                                                                                                  >  3:66842/1‑147 (MQ=255)
                        ccGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTACCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGa                                                                                                                              >  4:182249/1‑149 (MQ=255)
                          gTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGAc                                                                                                                             >  5:379059/1‑148 (MQ=255)
                               cATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGa                                                                                                                           >  1:156540/1‑145 (MQ=255)
                               cATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGa                                                                                                                           <  2:156540/145‑1 (MQ=255)
                               cATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGc                                                                                                                         <  5:370544/147‑1 (MQ=255)
                               cATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCt                                                                                                                       >  4:376639/1‑149 (MQ=255)
                                aTCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCtt                                                                                                                      <  6:379059/149‑1 (MQ=255)
                                aTCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCt                                                                                                                       >  2:349702/1‑148 (MQ=255)
                                       ggcagacGCGGCCTGTGCATGACCCAGCTCGTCTACTGCTCGAGGAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCTGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCTGGTAGACGAGCCTTACATCCt                                                                                                               <  2:178608/144‑1 (MQ=255)
                                         cacaCGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTAc                                                                                                                    >  5:445926/1‑142 (MQ=255)
                                             cgcgGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGt                                                                                                         <  5:303029/149‑1 (MQ=255)
                                              gctgCCAGTGCATGAGCCAGATCTTCTACTTCTCCAGCAGCTCCGAAAACACGAAGCTTTTTTTGGAACGTTTAGGTCTGCGCGCGGTGCGCATCCCGCTCAATGATCGGGATCGGATTCAGGTAGACGAGCGTTACATCCTGATCGTg                                                                                                        <  4:135619/146‑1 (MQ=255)
                                              gcgGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGAt                                                                                                                                       <  7:176576/118‑1 (MQ=255)
                                                ggCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGcc                                                                                                      >  6:14634/1‑149 (MQ=255)
                                                ggCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGcc                                                                                                      >  6:212373/1‑149 (MQ=255)
                                                ggCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGcc                                                                                                      <  5:212373/149‑1 (MQ=255)
                                                 gCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGccc                                                                                                     >  8:454282/1‑149 (MQ=255)
                                                     gTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCtt                                                                                                 <  8:149231/149‑1 (MQ=255)
                                                           gAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGCGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGCGCCCTCTTAcggcg                                                                                           >  6:219826/1‑149 (MQ=255)
                                                            aGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTAcggcgg                                                                                          >  6:334516/1‑149 (MQ=255)
                                                             gCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGcc                                                                                                                        >  1:21850/1‑118 (MQ=255)
                                                             gCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGcc                                                                                                                        <  2:21850/118‑1 (MQ=255)
                                                                     gTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTAc                                                                                 >  6:269363/1‑149 (MQ=255)
                                                                     gTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTATTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGCGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTAc                                                                                 >  6:93642/1‑149 (MQ=255)
                                                                      tCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTAcggcggcgg                                                                                       <  3:175281/142‑1 (MQ=255)
                                                                      tCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTAcggcggcgg                                                                                       >  4:175281/1‑142 (MQ=255)
                                                                        tACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGcc                                                                                                                        <  8:278492/107‑1 (MQ=255)
                                                                        tACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGcc                                                                                                                        >  7:278492/1‑107 (MQ=255)
                                                                          cTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCt                                                                             <  3:376639/148‑1 (MQ=255)
                                                                           ttCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTAcggcggcggcgg                                                                                    >  6:98622/1‑140 (MQ=255)
                                                                           ttCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTAcggc                                                                                            <  2:17463/132‑1 (MQ=255)
                                                                           ttCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTAcggc                                                                                            >  1:17463/1‑132 (MQ=255)
                                                                           ttCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTgg                                                                           >  6:68670/1‑149 (MQ=255)
                                                                           ttCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATAGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGATCAATGAGCGGGAACGGATTAAGGTAGACGAGCCTTACATACTGATCGAGCCCTCTTACGGCGGCGGCGGTACGGCTgg                                                                           >  5:237269/1‑149 (MQ=255)
                                                                                 agcagcTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGAGGTGCGCATCCCGCTCAATGAGCGGGGACGGATTCAGGTAGACGAGCCTTACATCCTGAACGTCCCCTCTTACGTCGGCGGCGGTACGGCTGGagcg                                                                       >  6:305131/1‑147 (MQ=255)
                                                                                   cagcTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGc                                                                   >  7:395346/1‑149 (MQ=255)
                                                                                   cagcTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGc                                                                   >  1:274906/1‑149 (MQ=255)
                                                                                     gcTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCa                                                                 <  4:324037/149‑1 (MQ=255)
                                                                                       tCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCGCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCAc                                                                >  3:226688/1‑148 (MQ=255)
                                                                                       tCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACg                                                               >  1:97107/1‑149 (MQ=255)
                                                                                         cGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGa                                                              >  4:98450/1‑148 (MQ=255)
                                                                                         cGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGAc                                                             <  8:183593/149‑1 (MQ=255)
                                                                                             aaCACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGt                                                         <  7:454282/149‑1 (MQ=255)
                                                                                             aaCACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGt                                                         <  1:349702/149‑1 (MQ=255)
                                                                                               ctcGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCTTTTTGCATGCCGCTATTTGAGCTGGAATGGATTCTGGTAGACGAGCCTTACATCCTGTTCGTGGCCTCTTTCGGCGGCGGATGTAAGGCTGGCGCGGTGCCACGAGAGGTaa                                                       <  8:154516/147‑1 (MQ=255)
                                                                                                 cGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCa                                                                 <  7:281510/137‑1 (MQ=255)
                                                                                                 cGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCa                                                                 >  8:281510/1‑137 (MQ=255)
                                                                                                    aGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGc                                                  >  3:370189/1‑149 (MQ=255)
                                                                                                    aGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGc                                                  >  3:415352/1‑149 (MQ=255)
                                                                                                     gCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCGCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCt                                                 <  4:226688/149‑1 (MQ=255)
                                                                                                      tgTTTTCTCGAACGGTTCGCTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTCGACGAGCCTTACATCCTGCTCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGc                                                  <  4:204347/146‑1 (MQ=255)
                                                                                                      cGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCtt                                                <  7:133577/149‑1 (MQ=255)
                                                                                                       gTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCttt                                               <  8:395346/149‑1 (MQ=255)
                                                                                                        ttttATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCtttt                                              >  2:201392/1‑149 (MQ=255)
                                                                                                        ttttATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATACAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACCGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCtag                                               >  3:204347/1‑146 (MQ=255)
                                                                                                          ttATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCttttt                                             <  4:370189/148‑1 (MQ=255)
                                                                                                          ttATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCttttt                                             <  2:97107/148‑1 (MQ=255)
                                                                                                           tATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTa                                            <  5:14634/148‑1 (MQ=255)
                                                                                                             tCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTc                                                    >  5:188168/1‑138 (MQ=255)
                                                                                                             tCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTc                                                    <  6:188168/138‑1 (MQ=255)
                                                                                                             tCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTaaa                                          <  4:415352/148‑1 (MQ=255)
                                                                                                             tCGAACGTTTAGGTCTGCCCCCCGAGCGCATCCCGCTCAATGAGCGCGAACGGATTCAGCTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCCGTACCGCTGGCGCGGTGCCACGACAGGTAATTCGCTATTTAAac                                         >  4:341839/1‑149 (MQ=255)
                                                                                                               gAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAGacga                                       >  1:48515/1‑149 (MQ=255)
                                                                                                               gAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAacga                                       >  3:190461/1‑149 (MQ=255)
                                                                                                               gAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAacga                                       >  1:93092/1‑149 (MQ=255)
                                                                                                                  ggTTTAGGTCTGCCCGCGGTGCGCAGACCGCTCAATGAGCGGGACCGTATTAAGGTCCACGTGCCTTACAAACTGATCTTGCCCTCTTCCGGCGGCGGCGGTTCGGCTGGCGCGGTGCAACTACAGGTAATTGCCTTTTTAAacgacta                                    <  6:237269/148‑3 (MQ=255)
                                                                                                                  cGTTTAGGTCTGCCCGCGGTGCGCATACCGCTCAATGAGCGGGAACGGATTCAGTTAGACGAGCCTTAAATCCTGATCGTGCCCTCTTACGGCGGCGGCTGTACGGCTGGCGCGGTGCCACTACAGGTAATTCGCTTTTTAAAcgacga                                    >  6:398994/1‑149 (MQ=255)
                                                                                                                   gTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAg                                   >  8:243126/1‑149 (MQ=255)
                                                                                                                   gTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAg                                   >  5:367542/1‑149 (MQ=255)
                                                                                                                   gTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACCGCGCCAGCCGTACCGCCGCCGCCGt                                                                     <  3:382806/115‑1 (MQ=255)
                                                                                                                   gTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACCGCGCCAGCCGTACCGCCGCCGCCGt                                                                     >  4:382806/1‑115 (MQ=255)
                                                                                                                        ggTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGCGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGcaca                               <  5:219826/148‑1 (MQ=255)
                                                                                                                         gTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAAc                             >  7:300656/1‑149 (MQ=255)
                                                                                                                              cccGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATc                                                                                                                 <  2:303388/60‑1 (MQ=255)
                                                                                                                              cccGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATc                                                                                                                 >  1:303388/1‑60 (MQ=255)
                                                                                                                                cgcgGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGt                      <  5:68670/149‑1 (MQ=255)
                                                                                                                                    gTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCt                  >  7:156903/1‑149 (MQ=255)
                                                                                                                                     tGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCtt                 >  1:341692/1‑149 (MQ=255)
                                                                                                                                        gcATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCCGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTcgc              >  2:266646/1‑149 (MQ=255)
                                                                                                                                          aTCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCgcgg            >  8:278975/1‑149 (MQ=255)
                                                                                                                                           tCCCGCTCATTGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCgcggc           >  1:47031/1‑149 (MQ=255)
                                                                                                                                             ccGCTCATTGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGt         <  5:39614/149‑1 (MQ=255)
                                                                                                                                               gCTCATTGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTa       <  2:47031/149‑1 (MQ=255)
                                                                                                                                               gCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTa       >  3:357996/1‑149 (MQ=255)
                                                                                                                                                   aaTGAGCGGTAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCTGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGAGTTATTGc   <  6:367542/149‑1 (MQ=255)
                                                                                                                                                    aTGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCt  >  1:164111/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGGTTTCCGTCCGGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCT  >  NC_000913/2800908‑2801204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: