Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,068,929 A→C 6.3% intergenic (‑121/+18) argO ← / ← mscS arginine transporter/mechanosensitive channel protein, small conductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,068,9290AC 6.3% 287.1 / 9.0 92intergenic (‑121/+18)argO/mscSarginine transporter/mechanosensitive channel protein, small conductance
Reads supporting (aligned to +/- strand):  ref base A (43/43);  new base C (4/2);  total (47/45)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

CAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGTTTTGCAGATCGC  >  NC_000913/3068781‑3069077
                                                                                                                                                    |                                                                                                                                                    
cAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtatta                                                                                                                                                      <  8:341221/149‑1 (MQ=255)
    aCCTTGAAAGTAATAAGAAAACACGTTGGAAATTCCGATTAAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATTAGAGAGAGGCCAGAGAGTATTAtctg                                                                                                                                                  >  2:445683‑M2/1‑145 (MQ=255)
      cTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTAtctggc                                                                                                                                                >  1:276565‑M2/1‑143 (MQ=255)
       ttGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTAtctggcc                                                                                                                                               <  8:248591‑M2/149‑8 (MQ=255)
       acgatattaataagaaaacaCGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTAtctggc                                                                                                                                                >  5:36710‑M2/8‑142 (MQ=255)
         gAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCtctggcc                                                                                                                                               >  3:137716‑M2/1‑140 (MQ=255)
         gAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTAtctggcct                                                                                                                                              <  7:230194‑M2/148‑9 (MQ=255)
          aaaGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCa                                                                                                                                            <  5:336156/149‑1 (MQ=255)
           aaGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAg                                                                                                                                           >  1:220181/1‑149 (MQ=255)
           aaGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAg                                                                                                                                           <  2:28864/149‑1 (MQ=255)
           aaGTAATAAGAAAACACGTTGGATAGTCCGAATTAAGTATCTGATGCAGAGAATATATCGCGGGATTGTTAGTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTTATAAGAGAGAGTCCAGAGAGTATTATCTGGCCTCAg                                                                                                                                           >  1:346235/1‑149 (MQ=255)
              taataaGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGt                                                                                                                                        <  4:244494/149‑1 (MQ=255)
               aataaGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTAtctggc                                                                                                                                                <  6:36710‑M2/140‑7 (MQ=255)
               aataaGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGtt                                                                                                                                       >  6:293125/1‑149 (MQ=255)
                ataaGATAACTCGTTTGATATTCTGAATTAAGTATCTTATGGAGAGAATATTTCGCTGGATTGTTATTAGCGTAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTAAGCGtt                                                                                                                                       <  6:394178/148‑1 (MQ=255)
                 taaGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGa                                                                                                                                     <  2:150202/149‑1 (MQ=255)
                    gAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTa                                                                                                                                  >  8:75485/1‑149 (MQ=255)
                      aaaCACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACg                                                                                                                                <  2:212325/149‑1 (MQ=255)
                      aaaCACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACg                                                                                                                                <  8:182981/149‑1 (MQ=255)
                        acacGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCa                                                                                                                              <  7:23063/149‑1 (MQ=255)
                                gATATTCCGAATTAAGTATCTGATGCCGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGtc                                                                                                                      <  8:294886/149‑1 (MQ=255)
                                gATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGtc                                                                                                                      >  3:234370/1‑149 (MQ=255)
                                gATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGtc                                                                                                                      >  5:349882/1‑149 (MQ=255)
                                gATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGtc                                                                                                                      >  6:411948/1‑149 (MQ=255)
                                  tatTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGtctt                                                                                                                    <  7:248591/149‑1 (MQ=255)
                                   atTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGtcttc                                                                                                                   >  1:267404/1‑149 (MQ=255)
                                    ttCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGtcttc                                                                                                                   <  7:337597/148‑1 (MQ=255)
                                      ccGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTcttctt                                                                                                                 <  7:75485/148‑1 (MQ=255)
                                      ccGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTcttctt                                                                                                                 <  6:349882/148‑1 (MQ=255)
                                            tAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCtctggcctctctcttattagtttttctgattgccaattaatat                                                                                                           <  2:336569‑M2/148‑44 (MQ=255)
                                              aGTATCTGCTGCAGAGACTAAATCGCGGGATTGTTATTCGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCtctggcctctctcttattagtttttctgattgccaattaatattat                                                                                                        >  3:326604‑M2/1‑103 (MQ=255)
                                              aGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCt                                                                                                        >  1:3556/1‑149 (MQ=255)
                                                tATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCt                                                                                                                           <  2:369183/128‑1 (MQ=255)
                                                tATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCt                                                                                                                           >  1:369183/1‑128 (MQ=255)
                                                  tCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACACGCTTa                                                                                                      >  5:359124/1‑147 (MQ=255)
                                                   cTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAg                                                                                                   >  6:328837/1‑149 (MQ=255)
                                                    tGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGt                                                                                                  >  8:445115/1‑149 (MQ=255)
                                                     gATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGtt                                                                                                 >  1:84781/1‑149 (MQ=255)
                                                      aTGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTaaa                                                                                                    <  4:234370/145‑1 (MQ=255)
                                                      aTGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTaaa                                                                                                    <  2:3556/145‑1 (MQ=255)
                                                      aTGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGtt                                                                                                 <  8:265084/148‑1 (MQ=255)
                                                      aTGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGt                                                                                                  <  2:357524/147‑1 (MQ=255)
                                                        gCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCtctggcctctctcttattagtttttctgattgccaattaatattatcaatttccgc                                                                                              >  1:336569‑M2/1‑93 (MQ=255)
                                                        gCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTca                                                                                               >  4:366439/1‑148 (MQ=255)
                                                                    tCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCgg                                                                                  >  2:353601/1‑149 (MQ=255)
                                                                        gggATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGc                                                                                                                               <  8:55680/100‑1 (MQ=255)
                                                                        gggATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGc                                                                                                                               >  7:55680/1‑100 (MQ=255)
                                                                        gggATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTAc                                                                              <  5:328837/149‑1 (MQ=255)
                                                                         ggAATGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCCCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACg                                                                             <  2:273611/149‑1 (MQ=255)
                                                                            ttGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGa                                                                          <  3:366439/149‑1 (MQ=255)
                                                                              gTTATTAGCGGAAATTTATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCATTGTCTTCTTTAACCCGCTTAAAGTTCACATCCATTTGCGGTTACGGGAAg                                                                        >  4:145985/1‑149 (MQ=255)
                                                                               ttattaGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCg                                                                                   >  1:166421/1‑137 (MQ=255)
                                                                               ttattaGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCg                                                                                   <  2:166421/137‑1 (MQ=255)
                                                                               ttattaGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGc                                                                       >  6:453788/1‑149 (MQ=255)
                                                                                 attaGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCt                                                                      <  1:353601/148‑1 (MQ=255)
                                                                                  ttaGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGa                                                                    <  5:293125/149‑1 (MQ=255)
                                                                                     gCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGtcttctgtg                                                                                                               >  6:6503/1‑100 (MQ=255)
                                                                                     gCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCt                                                                      <  7:352032/144‑1 (MQ=255)
                                                                                     gCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAAAGAGTATTATCTTGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTATCACCCGCTTAAAGTTCACATCCATATGCGGGTACGGGAAGca                                                                      >  8:352032/1‑143 (MQ=255)
                                                                                         aaaTTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACcggc                                                             <  8:230194/149‑1 (MQ=255)
                                                                                           aTTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACcggcgg                                                           >  2:109721/1‑149 (MQ=255)
                                                                                              gATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCAt                                                        >  1:100977/1‑149 (MQ=255)
                                                                                                taataTTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACcggcg                                                            <  6:202916/143‑1 (MQ=255)
                                                                                                taataTTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACcggcg                                                            >  5:202916/1‑143 (MQ=255)
                                                                                                taataTTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATc                                                       >  1:55426/1‑148 (MQ=255)
                                                                                                  atatTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCaaa                                                    <  5:411948/149‑1 (MQ=255)
                                                                                                    atTAATTGGCAATCAGAAAAACTAATCAGAGACACCCCAGAAAAAACTCtctcccctctctcttattagtttttctgattgccaattaatattatcaatttccgctaataacaatcccgcgatatagtctctgcatcagatacttaatt                                                  >  7:275422‑M2/1‑49 (MQ=255)
                                                                                                    atTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATAc                                                                 >  2:241796/1‑134 (MQ=255)
                                                                                                    atTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATAc                                                                 <  1:241796/134‑1 (MQ=255)
                                                                                                        aTTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACg                                              >  5:31976/1‑149 (MQ=255)
                                                                                                        aTTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACg                                              >  1:426050/1‑149 (MQ=255)
                                                                                                         ttGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCAc                                               <  1:458495/147‑1 (MQ=255)
                                                                                                         ttGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCAc                                               >  2:458495/1‑147 (MQ=255)
                                                                                                         ttGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGt                                             >  1:384945/1‑149 (MQ=255)
                                                                                                             cAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTaa                                         <  2:55426/149‑1 (MQ=255)
                                                                                                               aTCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATa                                       <  2:84781/149‑1 (MQ=255)
                                                                                                                tCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGc                                                                       >  8:66285/1‑116 (MQ=255)
                                                                                                                tCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGc                                                                       <  7:66285/116‑1 (MQ=255)
                                                                                                                tCAGAAAAACTAAAAAGAGAGAGGCCAGAGAGTATTATATGTACACATCGTTAATTAAGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACAACCATTTGCGGGTACGGGAAGCAGATACCGGCGGAATCAAATTCACGTTTAATaa                                      >  4:242129/1‑148 (MQ=255)
                                                                                                                       aaCTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAg                               <  2:220181/149‑1 (MQ=255)
                                                                                                                             taaGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATc                         >  3:396399/1‑149 (MQ=255)
                                                                                                                                gagagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCa                      <  7:184074/149‑1 (MQ=255)
                                                                                                                                 agagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAg                     <  5:453788/149‑1 (MQ=255)
                                                                                                                                  gagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGt                    <  2:100977/149‑1 (MQ=255)
                                                                                                                                     agGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTaca                 >  8:146665/1‑149 (MQ=255)
                                                                                                                                        ccAGAGAGTATTCTCGGGCCGCAGCGTTGATGACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGCTACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGt              <  2:346235/149‑1 (MQ=255)
                                                                                                                                         cAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTTAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGtt             >  4:399239/1‑149 (MQ=255)
                                                                                                                                         cAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGtt             >  3:52596/1‑149 (MQ=255)
                                                                                                                                         cAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGtt             >  7:349362/1‑149 (MQ=255)
                                                                                                                                          agagagTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGttt            >  7:278852/1‑149 (MQ=255)
                                                                                                                                            agagTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGt                                             >  4:313370/1‑114 (MQ=255)
                                                                                                                                            agagTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGt                                             <  3:313370/114‑1 (MQ=255)
                                                                                                                                             gagTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGTTTTGc         <  8:278852/149‑1 (MQ=255)
                                                                                                                                                    atCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTaca                 >  1:129923/1‑134 (MQ=255)
                                                                                                                                                    atCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTaca                 <  2:129923/134‑1 (MQ=255)
                                                                                                                                                    atCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGTTTTGCAGATcgc  >  3:357843/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGTTTTGCAGATCGC  >  NC_000913/3068781‑3069077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: