Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,109,464 C→T 5.6% intergenic (‑309/‑89) speC ← / → yqgA ornithine decarboxylase, constitutive/DUF554 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,109,4640CT 5.6% 338.3 / 6.2 109intergenic (‑309/‑89)speC/yqgAornithine decarboxylase, constitutive/DUF554 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (48/53);  new base T (3/3);  total (52/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01

Only 100 of 110 total aligned reads displayed.

CACCGCATGAGCAGTAACATAGAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGG  >  NC_000913/3109318‑3109608
                                                                                                                                                  |                                                                                                                                                
cACCGCATGAGCAGTAACATAGAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGc                                                                                                                                                  <  4:260480/147‑1 (MQ=255)
 aCCGCATGAGCAGTAACATAGAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGGAAg                                                                                                                                               >  7:128327/1‑149 (MQ=255)
  cctcATGAGCAGTAACATAGAAAGGGAAACGTTTCGCGCGAAACGGAGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAAGCATGGCACCTTTCGCTAAAGCGTGCAAGc                                                                                                                                              <  1:320678/146‑1 (MQ=255)
  ccGCATGAGCAGTAACATAGAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGc                                                                                                                                              <  5:154757/149‑1 (MQ=255)
    gCATGAGCAGTAACATAGAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACACCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGccg                                                                                                                                            <  7:204167/149‑2 (MQ=255)
     cATGAGCAGTAGCATAGAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCCCCTTTCGCTAAAGCGTGCACGcccg                                                                                                                                           >  8:112560/1‑147 (MQ=255)
     cATGAGCAGTAACATAGAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAg                                                                                                                                           <  2:151665/149‑1 (MQ=255)
          gCAGTAACATAGAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCa                                                                                                                                            >  1:109480/1‑143 (MQ=255)
          gCAGTAACATAGAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCa                                                                                                                                            <  2:109480/143‑1 (MQ=255)
           cAGTAACATAGAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGTCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACttt                                                                                                                                     <  1:80751/149‑1 (MQ=255)
           cAGTAACATAGAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACttt                                                                                                                                     >  1:204870/1‑149 (MQ=255)
            aGTAACATAGAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGGAAGCCAGGACTTTg                                                                                                                                    <  8:128327/149‑1 (MQ=255)
            aGTAACATAGAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTg                                                                                                                                    >  8:158070/1‑149 (MQ=255)
            aGTAACATAGAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTg                                                                                                                                    >  5:140276/1‑149 (MQ=255)
             gTAACATAGAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGc                                                                                                                                   <  8:58268/149‑1 (MQ=255)
                aCATAGAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGaa                                                                                                                                <  4:311437/149‑1 (MQ=255)
                 cATAGAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGaac                                                                                                                               <  2:204870/149‑1 (MQ=255)
                  aTAGAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGaac                                                                                                                               >  3:308940/1‑148 (MQ=255)
                   tAGAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGaaca                                                                                                                              >  8:308192/1‑148 (MQ=255)
                    aGAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGaacaaa                                                                                                                            >  1:152645/1‑149 (MQ=255)
                    aGAAAGGGAAACGTTTCGCCCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTACGTACAGAGCGGAGAAAATTCGCGCAATCATTGCACCTTTCGGTATAGCGTGCAATCCAGGACTTTGCg                                                                                                                                  >  5:403976/1‑143 (MQ=255)
                     gAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGaacaaa                                                                                                                            >  3:290573/1‑148 (MQ=255)
                      aaaGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGTAAGCCAGGACTTTGCGaacaaaca                                                                                                                          >  2:335756/1‑149 (MQ=255)
                         gggAAACGTTTCGCTCGAAACGGGGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCTCAATCATGGCTCCTTTCGCTAAAGCGTGCTAGCCAGGACTTTGCGAACAAACAAGc                                                                                                                       <  5:191521/149‑1 (MQ=255)
                         gggAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGc                                                                                                                       <  7:112560/149‑1 (MQ=255)
                          ggAAACGTTTCGCGCGAAAGGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACTGAGAGGAGAAAATTCGAGCAATGATGGCACCTTTCGCTAAAGCGGGCAAGCCAGGACTTTGCGAACAAACAAGc                                                                                                                       <  4:296711/148‑1 (MQ=255)
                           gAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCa                                                                                                                     >  3:173252/1‑149 (MQ=255)
                           gAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCa                                                                                                                     >  5:395651/1‑149 (MQ=255)
                            aaaCGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGTAGGCCAGGACTTTGCGAacaaacaa                                                                                                                         <  8:260642/144‑1 (MQ=255)
                            aaaCGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGTAGGCCAGGACTTTGCGAacaaacaa                                                                                                                         >  7:260642/1‑144 (MQ=255)
                            aaaCGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGTAGGCCAGGACTTTGCGAacaaacaa                                                                                                                         >  7:253907/1‑144 (MQ=255)
                            aaaCGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGTAGGCCAGGACTTTGCGAacaaacaa                                                                                                                         <  8:253907/144‑1 (MQ=255)
                            aaaCGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGc                                                                                                                                              >  8:453143/1‑123 (MQ=255)
                            aaaCGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGc                                                                                                                                              <  7:453143/123‑1 (MQ=255)
                            aaaCGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCAt                                                                                                                    >  7:257488/1‑149 (MQ=255)
                            aaaCGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCAt                                                                                                                    <  7:158070/149‑1 (MQ=255)
                                           aaCGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGTAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACg                                                                                                     <  1:335756/149‑1 (MQ=255)
                                              ggCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTc                                                                                                  <  2:152645/149‑1 (MQ=255)
                                                   tAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCataat                                                                                             <  4:391398/149‑1 (MQ=255)
                                                   tAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCataa                                                                                              >  6:162981/1‑148 (MQ=255)
                                                      gCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCATTCATGGCACCTTTCGCTAAACCGTGCAAGCCAGGACTTTGCTAACAAACAAGCCATCGACGGCACCAAACGGTCataat                                                                                             >  6:252873/1‑146 (MQ=255)
                                                       cGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCa                                                                                                 <  1:86770/141‑1 (MQ=255)
                                                       cGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCa                                                                                                 >  2:86770/1‑141 (MQ=255)
                                                           agaATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAAt                                                                                     <  5:20609/149‑1 (MQ=255)
                                                             aaTGTAAGGCGCTGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCAGCCACGGCACCCAACGGTCATAATAAGaaaaac                                                                                    >  4:274802/1‑146 (MQ=255)
                                                              aTGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCaa                                                                                  <  7:308192/149‑1 (MQ=255)
                                                               tGTAAGGAGATGGCGTGCATCGGTCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCaaa                                                                                 <  4:290573/149‑1 (MQ=255)
                                                               tGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCaaa                                                                                 <  8:257488/149‑1 (MQ=255)
                                                               tGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGGCATAATAAGAAAATGaaa                                                                                 <  1:94885/149‑1 (MQ=255)
                                                                     gagaTGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATa                                                                            >  2:41874/1‑148 (MQ=255)
                                                                      agaTGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATa                                                                            >  7:460693/1‑147 (MQ=255)
                                                                      agaTGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACa                                                                          <  3:5524/149‑1 (MQ=255)
                                                                         tGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGc                                                                        >  5:295051/1‑148 (MQ=255)
                                                                           gCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTaa                                                                     <  6:140276/149‑1 (MQ=255)
                                                                             gTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAAt                                                                                     <  6:377324/131‑1 (MQ=255)
                                                                             gTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAAt                                                                                     >  5:377324/1‑131 (MQ=255)
                                                                             gTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTaa                                                                     >  4:368812/1‑147 (MQ=255)
                                                                             gTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACa                                                                   <  1:301043/149‑1 (MQ=255)
                                                                              tGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAg                                                                  <  8:460693/149‑1 (MQ=255)
                                                                                    gggCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAAct                                                             <  5:248659/148‑1 (MQ=255)
                                                                                        aaaCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTtgt                                                        >  5:266301/1‑149 (MQ=255)
                                                                                        aaaCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTtgt                                                        <  4:173252/149‑1 (MQ=255)
                                                                                        aaaCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTtgt                                                        <  8:341975/149‑1 (MQ=255)
                                                                                         aaCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTtgtg                                                       <  3:368812/149‑1 (MQ=255)
                                                                                            tCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGt                                                     >  7:207801/1‑148 (MQ=255)
                                                                                            tCCGTACAGAGAGGAGAAAATTCGCGCAATCATGCCACATTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGTTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGt                                                     >  2:177115/1‑148 (MQ=255)
                                                                                                 aCAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCgg                                               >  4:187014/1‑149 (MQ=255)
                                                                                                  cAGAGAGGAGATAATTCGCGCAATCATGGCACCTTTGGCTAAAGCGTGCAAGCCATGAGTTTGCGAACAAACAAGCCATCGACGGAACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGc                                              <  6:120143/149‑1 (MQ=255)
                                                                                                   agagagGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCg                                                <  4:35984/146‑1 (MQ=255)
                                                                                                   agagagGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCg                                                >  3:35984/1‑146 (MQ=255)
                                                                                                   agagagGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGcc                                             >  7:387838/1‑149 (MQ=255)
                                                                                                    gagagGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGccc                                            >  2:268403/1‑149 (MQ=255)
                                                                                                       aggagAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGCACAAACAAGCCCTCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACCGCTAACAGGACCTCTGGTGGTCTTCGGccggtt                                         >  4:342130/1‑145 (MQ=255)
                                                                                                         gagaAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGc                                                                        <  7:165613/116‑1 (MQ=255)
                                                                                                         gagaAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGc                                                                        >  8:165613/1‑116 (MQ=255)
                                                                                                         gagaAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGc                                                                        >  8:258869/1‑116 (MQ=255)
                                                                                                         gagaAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAg                                                                  >  8:372756/1‑122 (MQ=255)
                                                                                                         gagaAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAg                                                                  <  7:372756/122‑1 (MQ=255)
                                                                                                          agaAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTAt                                      <  8:387838/149‑1 (MQ=255)
                                                                                                           gaAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCa                                                                                   <  8:350154/103‑1 (MQ=255)
                                                                                                              aaTTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCAtaataa                                                                                           <  1:376448/92‑1 (MQ=255)
                                                                                                              aaTTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCAtaataa                                                                                           >  2:376448/1‑92 (MQ=255)
                                                                                                                   gcgcACTCAGGGCACCTTTCGCTACAGCGTGCAAGCCAGCACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAg                             <  4:308940/149‑1 (MQ=255)
                                                                                                                      cAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGc                          <  1:268403/149‑1 (MQ=255)
                                                                                                                       aaTCATGGCACCTTTCGCTAAAGCGTGCAAGACAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAAACAAACAATACAGCTAACAGGAACTCTTGTGGTCATCCGCCCTTTTATCAACGCTATTg                           >  1:88053/1‑147 (MQ=255)
                                                                                                                            tGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCaaa                                                                                 >  4:222206/1‑88 (MQ=255)
                                                                                                                            tGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCaaa                                                                                 <  3:222206/88‑1 (MQ=255)
                                                                                                                            tGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTcg                     >  5:431720/1‑147 (MQ=255)
                                                                                                                               cACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACGGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACt                 <  3:274802/149‑1 (MQ=255)
                                                                                                                                 ccTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCACACGGGCATAATAAGAAACTCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTgg               <  1:274351/149‑1 (MQ=255)
                                                                                                                                 ccTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTgg               <  1:159786/149‑1 (MQ=255)
                                                                                                                                     tCGCTAAAGCGTGCTAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAAAGGTCTTAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGt             <  5:252873/147‑1 (MQ=255)
                                                                                                                                       gCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCAAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGc          <  5:162981/148‑1 (MQ=255)
                                                                                                                                         tAAAGCGTGCAAGCCAGGACTTTGCGAACAAACGAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTACTGCAGTCTTACTGGGTGGCGtt       >  2:97366/1‑149 (MQ=255)
                                                                                                                                         tAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGtt       >  5:213479/1‑149 (MQ=255)
                                                                                                                                         tAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGCGTGGCGtt       >  5:264245/1‑149 (MQ=255)
                                                                                                                                           aaGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGt             >  1:23593/1‑141 (MQ=255)
                                                                                                                                           aaGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGt             <  2:23593/141‑1 (MQ=255)
                                                                                                                                           aaGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTtct     >  1:205827/1‑149 (MQ=255)
                                                                                                                                              cGTGCAAGCCAGGACTTTGCGAACAAACGAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACATCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCgg  <  1:97366/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CACCGCATGAGCAGTAACATAGAAAGGGAAACGTTTCGCGCGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGG  >  NC_000913/3109318‑3109608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: