Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,291,109 A→T 5.9% K766* (AAA→TAA)  yraJ → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,291,1090AT 5.9% 287.6 / 2.2 94K766* (AAA→TAA) yraJputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (35/53);  new base T (4/2);  total (39/55)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAAT  >  NC_000913/3290968‑3291256
                                                                                                                                             |                                                                                                                                                   
gATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGtt                                                                                                                                              <  1:98637/149‑1 (MQ=255)
gATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGtt                                                                                                                                              >  3:363652/1‑149 (MQ=255)
gATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGtt                                                                                                                                              <  1:284781/149‑1 (MQ=255)
 aTACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTTCAGTCAGCGATCGCAACGGTAAACCGtt                                                                                                                                              <  8:244586/148‑1 (MQ=255)
 aTACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTg                                                                                                                                             <  3:145011/149‑1 (MQ=255)
  tactacGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGc                                                                                                                                            <  4:363652/149‑1 (MQ=255)
   actacGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGcc                                                                                                                                           >  5:210325/1‑149 (MQ=255)
    ctacGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGccc                                                                                                                                          <  6:425645/149‑1 (MQ=255)
     tacGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACATGTTACAGTCAGCGATCGCAACGGATTACCGTTGcga                                                                                                                                          >  2:67746/1‑146 (MQ=255)
       cGCAGCTGCCCGATAACGTCGATCTTGAACAAAGAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCTTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcga                                                                                                                                          <  1:67746/146‑3 (MQ=255)
        gCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCTACAGAGGTGCAATGGTAGCGGCGGGGTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTGAGCGATCGCATCGGTAAACCGTTGCCCTTTg                                                                                                                                      >  8:397205/1‑149 (MQ=255)
        gCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTg                                                                                                                                      <  1:225697/149‑1 (MQ=255)
        gCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTg                                                                                                                                      <  5:152890/149‑1 (MQ=255)
        gCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTg                                                                                                                                      <  4:105175/149‑1 (MQ=255)
                 cGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTg                                                                                                                             >  5:78525/1‑149 (MQ=255)
                  gATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGGTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGAGCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGc                                                                                                                            <  4:322113/149‑1 (MQ=255)
                    tAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCa                                                                                                                          <  4:324321/149‑1 (MQ=255)
                    tAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTAAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCa                                                                                                                          <  2:438258/149‑1 (MQ=255)
                        gTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCaa                                                                                                                      >  8:222470/1‑149 (MQ=255)
                        gTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCaa                                                                                                                      >  8:334112/1‑149 (MQ=255)
                             tCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgat                                                                                                                  >  3:267518/1‑148 (MQ=255)
                                tGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATa                                                                                                              >  3:164151/1‑149 (MQ=255)
                                 gAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGc                                                                                                                            <  7:343749/134‑1 (MQ=255)
                                 gAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGc                                                                                                                            >  8:343749/1‑134 (MQ=255)
                                 gAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgatgat                                                                                                               <  7:382264/147‑1 (MQ=255)
                                 gAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATa                                                                                                              <  6:187218/148‑1 (MQ=255)
                                 gAACAAACAACACAGTTCGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgat                                                                                                                  <  8:372742/144‑1 (MQ=255)
                                  aacaaacaACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACg                                                                                                            >  7:130877/1‑149 (MQ=255)
                                   acaaacaaCACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGTGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACgg                                                                                                           <  5:253917/149‑1 (MQ=255)
                                    caaacaaCACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACggg                                                                                                          >  1:195314/1‑149 (MQ=255)
                                    caaacaaCACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACggg                                                                                                          >  1:89180/1‑149 (MQ=255)
                                     aaacaaCACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACgggg                                                                                                         <  6:78525/149‑1 (MQ=255)
                                     aaacaaCACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTGGCGCTCTTGCCAGCAACGATGATACgggg                                                                                                         >  5:250467/1‑148 (MQ=255)
                                      aacaacACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGc                                                                                                        <  7:222470/149‑1 (MQ=255)
                                       acaactCAGTTTGTGGTGGCCTACAGTGGTGCAATGGTAGCGGGGCGTTTCTTCGGCAATATCGGTTATCGCGTACTTGTTTCAGGCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACgt                                                                                                           <  7:331608/145‑2 (MQ=255)
                                        caacaCAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGcaa                                                                                                      <  2:261774/149‑1 (MQ=255)
                                         aacaCAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACga                                                                                                                   <  8:236043/135‑1 (MQ=255)
                                         aacaCAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACga                                                                                                                   >  7:236043/1‑135 (MQ=255)
                                          acacAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGAGGATACGGGGcaac                                                                                                     >  3:222850/1‑148 (MQ=255)
                                           cacaGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGcaaca                                                                                                    >  8:430676/1‑148 (MQ=255)
                                            acaGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACaaa                                                                                                  <  6:210325/149‑1 (MQ=255)
                                            acaGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACaaa                                                                                                  <  4:267518/149‑1 (MQ=255)
                                            acaGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGGCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACaaa                                                                                                  <  2:170473/149‑1 (MQ=255)
                                              aGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGt                                                                                                <  6:399899/149‑1 (MQ=255)
                                               gTTTGTGGTGCCCAACATAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTa                                                                                               <  5:184400/149‑1 (MQ=255)
                                                  tgtgGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTAtc                                                                                             <  7:334112/148‑1 (MQ=255)
                                                  tgtgGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTAtc                                                                                             <  2:195314/148‑1 (MQ=255)
                                                         cgcAACAGAGGTGCATTGGTAGCGGCGCGTTTCAACGCCAAGATCGGTTATGGGGTACTTGTTACAGTCAGCGATCGCAAGGGTTATCCGTTGCCGTTTGGCGGTCTTGGCCGCGACGAGGGTACGGGGCAGGAAAGTAtcgtcggggt                                                                                     >  4:170640/3‑145 (MQ=255)
                                                         cgcAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGAt                                                                                       >  4:12510/3‑147 (MQ=255)
                                                         cccAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCTAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCa                                                                                                                          <  4:14022/112‑1 (MQ=255)
                                                         cccAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCTAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCa                                                                                                                          >  3:14022/1‑112 (MQ=255)
                                                         cccAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAATCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGa                                                                                     <  5:421742/149‑1 (MQ=255)
                                                                     gCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACgggg                                                                                                         <  3:180819/117‑1 (MQ=255)
                                                                     gCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACgggg                                                                                                         >  4:180819/1‑117 (MQ=255)
                                                                     gCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGa                                                                                        <  5:410814/134‑1 (MQ=255)
                                                                     gCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGa                                                                                        >  6:410814/1‑134 (MQ=255)
                                                                       attGGTAGCGGCGCGGTTCCGCGCCCCTATCGGTTATCGCGTACGTGTTGGAGTCAGGGCTCGCAAGGGTAATCCGTTGCGCGTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATGGTCGATGAGGGCGGCCTAcga                                                                        >  2:115492/3‑146 (MQ=255)
                                                                           gTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACaaa                                                                                                  <  2:319878/118‑1 (MQ=255)
                                                                           gTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACaaa                                                                                                  >  1:319878/1‑118 (MQ=255)
                                                                                 gcgcGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTgg                                                             <  7:430676/149‑1 (MQ=255)
                                                                                 gcgcGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCGTTGGCGCTCTTGCCAGCCACGATGATACGGGGCAACAAAGGATCGTGGAGGAGGGCGGCAGACGAGATCTGTCTgg                                                             >  4:283912/1‑149 (MQ=255)
                                                                                   gcgTTTCAACGCCAATATCGGTTATCGAGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGa                                                           >  1:297701/1‑149 (MQ=255)
                                                                                    cgTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGat                                                          <  3:283912/149‑1 (MQ=255)
                                                                                          aaCGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGa                                                     <  2:89180/148‑1 (MQ=255)
                                                                                          aaCGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAg                                                    <  8:130877/149‑1 (MQ=255)
                                                                                          aaCGCCAATATCGGTTATCCCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAg                                                    <  3:12510/149‑1 (MQ=255)
                                                                                          aaCGCCAATATCGGATATCGCGAACTAGTTACACTAAGCGATCCCAACCCAAAACCGTTTCCCTTTGGATCTCTTGCCAGCAACGATGATACGGTGCAACAAAGTATCGTCCATGAGGGCGGCATACAAAATCTATATGGGTTATCGAg                                                    >  8:451601/1‑149 (MQ=255)
                                                                                           aCGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCCACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCAGACTATATCTGACTGGGATATCGAg                                                    >  6:263107/1‑148 (MQ=255)
                                                                                           aCGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAg                                                    >  1:174203/1‑148 (MQ=255)
                                                                                                aaTATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAAt                                               >  1:407954/1‑148 (MQ=255)
                                                                                                 atatCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATca                                             >  5:284157/1‑149 (MQ=255)
                                                                                                       gTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGc                                       >  3:178573/1‑149 (MQ=255)
                                                                                                        ttATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCGTTGGCGCTCTTGGCAGGAACGAGGGTACGGGGCAACAATGTATCGTCGATGAGGGCGGCATACTTTATGTCTCTGGGTTATCggg                                                    >  2:208055/1‑133 (MQ=255)
                                                                                                        ttATCGCGTACTTGATACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTTTCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAg                                                    <  1:208055/135‑1 (MQ=255)
                                                                                                             gcgTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACt                                 >  3:392041/1‑149 (MQ=255)
                                                                                                             gcgTACCTGTTCCAGTCAGCGACCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATCCGGGCCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACt                                 <  7:210347/149‑1 (MQ=255)
                                                                                                               gTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGt                               <  6:284157/149‑1 (MQ=255)
                                                                                                                tACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTAc                             <  6:250467/149‑1 (MQ=255)
                                                                                                                      ttACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAACTCACAATGCTGGACTGTACGCTgg                        <  2:297701/149‑1 (MQ=255)
                                                                                                                          aGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGa                     >  1:171235/1‑148 (MQ=255)
                                                                                                                           ggcAGCGATCGCTACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGCGCAACAAAGTTTCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTggg                       <  6:424573/143‑1 (MQ=255)
                                                                                                                           gTCAGCGATCCCAACGGTAAACCGTTGCCCTTTGCCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCACTCTGGGATATCGAGTAAATCACAAAGCAGGACTGTACGCTggg                       >  5:424573/1‑145 (MQ=255)
                                                                                                                              aGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATc                 <  2:407954/148‑1 (MQ=255)
                                                                                                                              aGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGCGCAAATAAGTTTCGTCGATGAGGTCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCa                <  3:170640/149‑1 (MQ=255)
                                                                                                                               gCGATCGCAACTGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACTGCGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAg               <  5:263107/149‑1 (MQ=255)
                                                                                                                               caaagggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAAt                  >  8:204431/7‑146 (MQ=255)
                                                                                                                               caaagggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCa                <  7:204431/142‑1 (MQ=255)
                                                                                                                                cGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAgg              >  1:180605/1‑149 (MQ=255)
                                                                                                                                aaagggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAgg              >  8:352587/6‑149 (MQ=255)
                                                                                                                                 gATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGc             <  2:171235/149‑1 (MQ=255)
                                                                                                                                  aTCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCa            <  4:164151/149‑1 (MQ=255)
                                                                                                                                    cGCAACGGTACACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGAGGATACGGGGCAACAAAGTATCGTCGATGATGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGa          <  1:287203/149‑1 (MQ=255)
                                                                                                                                          ggTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATcaaca    <  7:397205/149‑1 (MQ=255)
                                                                                                                                            tAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTCTCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAAt  <  4:222850/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
GATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAAT  >  NC_000913/3290968‑3291256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: