Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,488,983 C→A 7.6% V400L (GTG→TTG)  argD ← bifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,488,9830CA 7.6% 237.8 / 6.4 74V400L (GTG→TTG) argDbifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (36/32);  new base A (3/3);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GACCGCATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCG  >  NC_000913/3488841‑3489124
                                                                                                                                              |                                                                                                                                             
gACCGCATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCCACCACCTTCGCCACCGCGTg                                                                                                                                         >  1:412850/1‑149 (MQ=255)
   cGCATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGc                                                                                                                                      >  5:350088/1‑149 (MQ=255)
         aCGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAAc                                                                                                                                 <  6:350088/148‑1 (MQ=255)
         aCGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACg                                                                                                                                >  3:121226/1‑149 (MQ=255)
         aCGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACg                                                                                                                                >  5:137056/1‑149 (MQ=255)
           gggATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGtt                                                                                                                              <  7:15861/149‑1 (MQ=255)
            ggATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCCACGCGGTGGCGaaggtggt                                                                                                                             <  1:160128/149‑7 (MQ=255)
             gATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGc                                                                                                                            <  7:68084/149‑1 (MQ=255)
                   gCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCt                                                                                                                      >  4:200589/1‑149 (MQ=255)
                     tGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCtt                                                                                                                     >  6:452080/1‑148 (MQ=255)
                       aGTCGCTAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCAt                                                                                                                  <  6:137056/149‑1 (MQ=255)
                           gCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGat                                                                                                              <  2:257009/149‑1 (MQ=255)
                           gCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGa                                                                                                               >  3:82757/1‑148 (MQ=255)
                            cAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGata                                                                                                             <  3:291515/149‑1 (MQ=255)
                              aGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATc                                                                                                           <  2:224834/149‑1 (MQ=255)
                                       aTTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTc                                                                                                  <  3:81845/149‑1 (MQ=255)
                                          cGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTcca                                                                                                <  2:43760/148‑1 (MQ=255)
                                               gCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTccaccacca                                                                                          <  3:5574/149‑1 (MQ=255)
                                                  tAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGCCATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGAAATCCGCATCTTCCACCACCAGc                                                                                        >  5:11523/1‑148 (MQ=255)
                                                     tGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACg                                                                                    <  5:353928/149‑1 (MQ=255)
                                                      gCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACgg                                                                                   >  1:249541/1‑149 (MQ=255)
                                                      gCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACgg                                                                                   >  2:23011/1‑149 (MQ=255)
                                                       cAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGt                                                                                  <  6:11523/149‑1 (MQ=255)
                                                            tAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGt                                                                                  >  1:28468/1‑144 (MQ=255)
                                                            tAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGt                                                                                  <  2:28468/144‑1 (MQ=255)
                                                            tAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCaa                                                                              >  7:300637/1‑148 (MQ=255)
                                                               aCCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGtt                                                                                                                              >  5:225627/1‑97 (MQ=255)
                                                               aCCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGtt                                                                                                                              <  6:225627/97‑1 (MQ=255)
                                                                 cATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCa                                                                        <  2:249541/149‑1 (MQ=255)
                                                                      gATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGCCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATcaca                                                                   >  7:338805/1‑149 (MQ=255)
                                                                            gCATTCACGCCGCATCCGACATCTTTTGCCTTAAGCTTCGTATTTACGCCCCTACCACCTTCGCCCCCGCGTTGGCGAAACGTTGCATCCCTTCATCTATATCCGCATCTTCCA‑CACCAGCGACGGTGCAAAGCGCATCACAtccggtc                                                            <  6:32397/149‑1 (MQ=255)
                                                                               ttCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCCACGCGGTGGCGaaggt                                                                                                                                >  7:201373/1‑76 (MQ=38)
                                                                               ttCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCCACGCGGTGGCGaaggt                                                                                                                                <  8:201373/79‑4 (MQ=38)
                                                                                   cgccgcATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCAt                                                      <  8:300637/149‑1 (MQ=255)
                                                                                    gccgcATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATc                                                                                                                 >  4:14209/1‑89 (MQ=255)
                                                                                    gccgcATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATc                                                                                                                 <  3:14209/89‑1 (MQ=255)
                                                                                    gccgcATCCGACATCTTTTGCCTGAGGCTTCGTTTTGACGCCCCAGACACCTTCGCCAACGCGTGGGCGAAACGGTGCCTACCGTCATCCATATCCGACTCTTCCACCACCAGCGACGGTGCAAAACGCATCACAtccgggccg                                                          >  7:324963/1‑144 (MQ=255)
                                                                                      cgcATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCAtt                                                     >  4:230858/1‑147 (MQ=255)
                                                                                         aTCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCa                                                <  8:322973/149‑1 (MQ=255)
                                                                                             gACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAg                                                  <  4:28481/143‑1 (MQ=255)
                                                                                             gACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAg                                                  >  3:28481/1‑143 (MQ=255)
                                                                                                   ttttGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCAc                                               >  6:139624/1‑140 (MQ=255)
                                                                                                   ttttGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCAc                                               <  5:139624/140‑1 (MQ=255)
                                                                                                          tGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCAt                                                                       >  4:122/1‑109 (MQ=255)
                                                                                                          tGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCAt                                                                       <  3:122/109‑1 (MQ=255)
                                                                                                                ttCGTATTTACGCCCCACCCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGccc                         >  7:280139/1‑149 (MQ=255)
                                                                                                                ttCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCAt                                                                       >  2:177594/1‑103 (MQ=255)
                                                                                                                ttCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCAt                                                                       <  1:177594/103‑1 (MQ=255)
                                                                                                                ttCGTATTAACACCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCCATATCCGCATCTTCCCCCACCAGCGACGGTGCAACACGCATCACATCCGCTCCGGCATTCCGCACCATTACGACCGCCTCTGCGccc                         >  4:81124/1‑149 (MQ=255)
                                                                                                                 tCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCg                        >  5:241668/1‑149 (MQ=255)
                                                                                                                 tCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCg                        >  4:393176/1‑149 (MQ=255)
                                                                                                                    tATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCa                      >  3:121238/1‑148 (MQ=255)
                                                                                                                        tACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACa                  <  5:367515/148‑1 (MQ=255)
                                                                                                                         aCGCCCCAACCCCCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTGCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCCGCACCATGACGCCAGCCTCTGCGGCCGCATACAgg                >  8:324378/1‑149 (MQ=255)
                                                                                                                          caccccAACCACCTTCGACACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCACTGCGCCCGCATACAgg                <  4:255112/146‑1 (MQ=255)
                                                                                                                          cGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCCTCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGa               <  2:412850/149‑1 (MQ=255)
                                                                                                                           gCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACAtcc                                                                <  1:452446/99‑1 (MQ=255)
                                                                                                                           gCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACAtcc                                                                >  2:452446/1‑99 (MQ=255)
                                                                                                                           gCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGa               >  6:52446/1‑148 (MQ=255)
                                                                                                                             cccAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCGTCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGGCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAAt            >  3:94094/1‑149 (MQ=255)
                                                                                                                             cccAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAAt            >  1:120062/1‑149 (MQ=255)
                                                                                                                             cccAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAAt            >  5:219224/1‑149 (MQ=255)
                                                                                                                             cccAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAAt            >  6:181397/1‑149 (MQ=255)
                                                                                                                               cAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCCTCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCCGCCTCTGCGCCCGCATACAGGAAATCa          >  4:75163/1‑149 (MQ=255)
                                                                                                                               cAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGCCGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATcc          >  4:90943/1‑148 (MQ=255)
                                                                                                                               cAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACAtc                                                                 >  8:435062/1‑94 (MQ=255)
                                                                                                                               cAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACAtc                                                                 <  7:435062/94‑1 (MQ=255)
                                                                                                                                 accaccTTCGCCTCCTCTTTGGCGAAACGTTGCATCCCTTCATCTATATCGGCATCTTCCACCACCAGCGACGGGTCAAAAAGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCAcg        <  4:94094/149‑1 (MQ=255)
                                                                                                                                    cgtttCGCCCACGCGGTGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTAc                                         >  8:156372/4‑113 (MQ=255)
                                                                                                                                    cgtttCGCCCACGCGGTGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTAc                                         <  7:156372/110‑1 (MQ=255)
                                                                                                                                    accTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTCCGCCAGCCTCTGCGCCCGCATACAGGAAATCACgcgc     <  4:82757/149‑1 (MQ=255)
                                                                                                                                      cTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGccc   >  2:212748/1‑149 (MQ=255)
                                                                                                                                         cGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCg  <  3:200589/147‑1 (MQ=255)
                                                                                                                                         cGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCg  <  4:121226/147‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                             
GACCGCATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCG  >  NC_000913/3488841‑3489124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑