BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,755,082	T→A	5.4%	Y477F (TAC→TTC)	aldB ←	aldehyde dehydrogenase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,755,082	0	T	A	5.4%	287.6 / 4.1	91	Y477F (TAC→TTC)	aldB	aldehyde dehydrogenase B
Reads supporting (aligned to +/- strand): ref base T (48/37); new base A (2/3); total (50/41)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 101 total aligned reads displayed.

ATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAG  >  NC_000913/3754935‑3755230

aTCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTa                                                                                                                                                     <  2:395610/149‑1 (MQ=255)

   aGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTa                                                                                                                                                     <  1:272671/146‑1 (MQ=255)

   aGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTa                                                                                                                                                     >  2:272671/1‑146 (MQ=255)

   aGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGc                                                                                                                                                   >  4:233092/1‑148 (MQ=255)

            aTACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAAcgc                                                                                                                                         >  7:273726/1‑149 (MQ=255)

                ccAATGCGACCAGCTTCTTATATGTGACCAGGCGCAACGGTTTATCCGAGTAGCTCACCAGCAGGCAGTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCa                                                                                                                                     <  1:44867/149‑1 (MQ=255)

                ccAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCa                                                                                                                                     >  7:243405/1‑149 (MQ=255)

                ccAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCa                                                                                                                                     >  2:31146/1‑149 (MQ=255)

                ccAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCa                                                                                                                                     >  8:322192/1‑149 (MQ=255)

                ccAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGACACCAACCGCCGCa                                                                                                                                     >  1:111913/1‑149 (MQ=255)

                      cGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGc                                                                                                                                <  1:312181/148‑1 (MQ=255)

                       gACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCg                                                                                                                              >  3:378490/1‑149 (MQ=255)

                                cttATCTCAGAACAGCCCCAACGGTTTATCCTAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGAACGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTaatc                                                                                                                       <  8:169911/147‑3 (MQ=255)

                                cttATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGc                                                                                                                       <  7:322192/147‑1 (MQ=255)

                                   atatCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGt                                                                                                                     <  7:53775/146‑1 (MQ=255)

                                   atatCAGAACAGCCCCAAAGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTg                                                                                                                    <  2:472269/147‑1 (MQ=255)

                                     atCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGa                                                                                                                   <  6:284038/146‑1 (MQ=255)

                                     atCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGa                                                                                                                   >  5:284038/1‑146 (MQ=255)

                                        aGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACa                                                                                                              <  4:85969/148‑1 (MQ=255)

                                         gcacacCCCCCACGGCTTCTCCGAGTAGCGCACCAGCAGGCCCTTCGTCTGCCGGTAATGCTCCAGCATCATCTTGTGGGGTTCGCGACCGATACCTGCGTGTTCGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGCTAACAga                                                                                                            <  6:4961/147‑2 (MQ=255)

                                         gAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGGACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGCAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGt                                                                                                            <  6:325486/149‑1 (MQ=255)

                                         gAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGt                                                                                                            <  1:137135/149‑1 (MQ=255)

                                         gAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCAGGGTAAGCGTGATAACAGt                                                                                                            >  3:22704/1‑149 (MQ=255)

                                          aaCAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCAGGGTAAGCGTGATAACAGtt                                                                                                           <  4:22704/149‑1 (MQ=255)

                                           aCAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTg                                                                                                          <  1:31146/149‑1 (MQ=255)

                                                cccAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCCCCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCca                                                                                                     >  1:204557/1‑149 (MQ=255)

                                                 ccAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTTGTTTGCTGGTAATGCTACAGCATCATCTTGTTGGTTTCGCGACCGATACCTGATTGTTTGAATCCAACAAACGCAGCATTTGCCgg                                                                                                                             >  4:362039/1‑124 (MQ=255)

                                                 ccAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCAt                                                                                                                                    <  3:156230/117‑1 (MQ=255)

                                                 ccAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCAt                                                                                                                                    >  4:156230/1‑117 (MQ=255)

                                                 ccAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGAAGCCACCAAACGCCGCATGTGCCgg                                                                                                                             >  4:368173/1‑124 (MQ=255)

                                                 ccAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGAAGCCACCAAACGCCGCATGTGCCgg                                                                                                                             <  3:362039/124‑1 (MQ=255)

                                                 ccAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGAAGCCACCAAACGCCGCATGTGCCgg                                                                                                                             <  3:368173/124‑1 (MQ=255)

                                                 caaaCGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGCCAGATACCAGATTGATTGTAGCCACCAAACGCCGCATCTGCCGGGTAAGAGTGATAACAGTTGGTCcac                                                                                                    >  8:208337/3‑149 (MQ=255)

                                                    aCGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACAcg                                                                                                 >  8:191309/1‑149 (MQ=255)

                                                            tCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGc                                                                                                                       <  5:340521/119‑1 (MQ=255)

                                                            tCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGc                                                                                                                       >  6:340521/1‑119 (MQ=255)

                                                            tCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGt                                                                                                            <  2:158154/130‑1 (MQ=255)

                                                            tCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGt                                                                                                            >  1:158154/1‑130 (MQ=255)

                                                                      tCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCcg                                                                               >  8:19241/1‑149 (MQ=255)

                                                                       cACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCcgc                                                                              >  6:343378/1‑149 (MQ=255)

                                                                        aCCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGcc                                                                                >  8:442015/1‑146 (MQ=255)

                                                                        aCCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGcc                                                                                <  7:442015/146‑1 (MQ=255)

                                                                            gcagGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGccc                                                                          >  6:305657/1‑148 (MQ=255)

                                                                            gcagGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGccc                                                                          >  4:419490/1‑148 (MQ=255)

                                                                               ggCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTgg                                                                                                         >  4:5151/1‑114 (MQ=255)

                                                                               ggCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATc                                                                      <  3:419490/149‑1 (MQ=255)

                                                                                gCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCt                                                                     >  4:427114/1‑149 (MQ=255)

                                                                                  aCTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGcc                                                                                <  5:60557/136‑1 (MQ=255)

                                                                                  aCTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGcc                                                                                >  6:60557/1‑136 (MQ=255)

                                                                                  aCTTGGATTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATAGTTTGTCGCCACCAAACGCCCCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGGGCCCCATCTta                                                                   >  3:224615/1‑149 (MQ=255)

                                                                                   cTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAg                                                                                                             >  8:335307/1‑106 (MQ=255)

                                                                                   cTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAg                                                                                                             <  7:335307/106‑1 (MQ=255)

                                                                                   cTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATc                                                                      <  5:218701/145‑1 (MQ=255)

                                                                                   cTTGGTTTGCTGGTAATCCACCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATc                                                                      >  6:218701/1‑145 (MQ=255)

                                                                                    ttGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTta                                                                   >  1:460302/1‑147 (MQ=255)

                                                                                    ttGGTTTGCTGGTAATGCTCCACAATCATCTTGTGGGTTTCTCGACCGATACCTTATTGTTTGAAGACACCAAACGCCGCATGTGCCGTGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTtata                                                                 <  7:208337/149‑1 (MQ=255)

                                                                                      ggTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTtata                                                                 <  8:243405/147‑1 (MQ=255)

                                                                                        tttGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACCCGCGCCCAGCCTGTATGCCGCGCCCCATCt                                                                     >  4:238126/1‑141 (MQ=255)

                                                                                        tttGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCt                                                                     <  3:238126/141‑1 (MQ=255)

                                                                                        tttGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGcc                                                             <  5:343378/149‑1 (MQ=255)

                                                                                        tttGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGcc                                                             <  3:427114/149‑1 (MQ=255)

                                                                                          tGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATATCAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTAATAGGCCa                                                            <  6:161053/148‑1 (MQ=255)

                                                                                            cTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGAt                                                         >  6:8128/1‑149 (MQ=255)

                                                                                               gTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGcc                                                                                        <  8:102535/115‑1 (MQ=255)

                                                                                               gTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGcc                                                                                        >  7:102535/1‑115 (MQ=255)

                                                                                                tAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTAcc                                                     <  7:19241/149‑1 (MQ=255)

                                                                                                  aTGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCg                                                    <  7:191309/148‑1 (MQ=255)

                                                                                                     cTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTg                                                 <  3:233092/148‑1 (MQ=255)

                                                                                                     cTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGc                                                <  5:305657/149‑1 (MQ=255)

                                                                                                     cTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGc                                                >  7:41307/1‑149 (MQ=255)

                                                                                                       ccAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGt                                                                                     <  6:261184/110‑1 (MQ=255)

                                                                                                       ccAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGt                                                                                     >  5:261184/1‑110 (MQ=255)

                                                                                                        cAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCgg                                              <  5:380997/148‑1 (MQ=255)

                                                                                                        cAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGc                                             >  1:229840/1‑149 (MQ=255)

                                                                                                           catcatCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGATGGTCCACACGCGCCCCGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTCCCGTTGCGGCTcc                                          >  2:341765/1‑149 (MQ=255)

                                                                                                            atcatcTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTc                                           >  2:368123/1‑147 (MQ=255)

                                                                                                             tcattTTCTGTGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTTCCGGGTAAGCTTGATAACAGTTTGTCCACTCTCGCGCAGCCTTTATGCcgctc                                                                            <  4:217140/113‑3 (MQ=255)

                                                                                                             tcatcTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCAACACGCGCCCAGCCTGTATGCCGCGACCCATCTTATAGGCCAGATTACCGTTTCGGCTc                                           >  3:357795/1‑146 (MQ=255)

                                                                                                             tcatcTTGTGGGTTTCGCGAACGATACATTATTGTATTTAGCCACCAAACGCCTAATGTGCCGGGTAAGCGTGATAAAAGTTGGTAAACACTAGCCCAGCATGTATGCCtcgc                                                                            >  3:217140/1‑113 (MQ=255)

                                                                                                               atcTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGa                                       <  8:273726/148‑1 (MQ=255)

                                                                                                                    gtgGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCcg                                 >  2:54351/1‑149 (MQ=255)

                                                                                                                        gTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACTGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGcc                             >  3:278906/1‑149 (MQ=255)

                                                                                                                         tttCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGcc                             >  1:38968/1‑148 (MQ=255)

                                                                                                                             gcgACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGc                        >  5:123387/1‑149 (MQ=255)

                                                                                                                             gcgACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGc                        >  5:37957/1‑149 (MQ=255)

                                                                                                                              cgACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGc                        >  8:387754/1‑148 (MQ=255)

                                                                                                                                      aCCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGc                >  4:372914/1‑148 (MQ=255)

                                                                                                                                        cTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTa             >  7:320691/1‑149 (MQ=255)

                                                                                                                                         tGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTAt            >  3:432012/1‑149 (MQ=255)

                                                                                                                                           aTTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATc           <  2:460302/148‑1 (MQ=255)

                                                                                                                                            ttgtttgtAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCg                                               <  7:196092/111‑1 (MQ=255)

                                                                                                                                            ttgtttgtAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCg                                               >  8:196092/1‑111 (MQ=255)

                                                                                                                                                 tgtAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGc     <  2:229840/148‑1 (MQ=255)

                                                                                                                                                 tgtAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGc     <  1:368123/148‑1 (MQ=255)

                                                                                                                                                  gtAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCa   >  8:51438/1‑149 (MQ=255)

                                                                                                                                                   tAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGTGGCTCCAGACGCCCGCGCTCAGGCCATATTGCGTATCGTTCGCCAg  >  2:24248/1‑149 (MQ=255)

                                                                                                                                                   tAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGc                >  6:121674/1‑135 (MQ=255)

                                                                                                                                                   tAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGc                <  5:121674/135‑1 (MQ=255)

                                                                                                                                                   tAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAg  >  7:430700/1‑149 (MQ=255)

                                                                                                                                                   tAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAg  <  6:123387/149‑1 (MQ=255)

ATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAG  >  NC_000913/3754935‑3755230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`