Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,964,929 C→T 100% E234E (GAG→GAA)  rhlB ← ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,964,9290CT100.0% 689.2 / NA 197E234E (GAG→GAA) rhlBATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (101/96);  total (101/96)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 200 total aligned reads displayed.

ACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGC  >  NC_000913/3964783‑3965073
                                                                                                                                                  |                                                                                                                                                
acacGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGttc                                                                                                                                                <  4:246553/149‑1 (MQ=255)
 cacGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGttct                                                                                                                                               <  3:242514/149‑1 (MQ=255)
  ccGATGACCATCTGCTGCCCGGTGGCCCCCGATCTCTTCCCAACGGTGTTTGGGGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTATTCGCTCAGCGGTTGCAGCAACAGCATTTTTTCTTCTTTAGAAGGGTAGAAAAGTtctt                                                                                                                                              >  6:410648/2‑149 (MQ=255)
  acGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTtctt                                                                                                                                              <  5:295/149‑1 (MQ=255)
   cGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGttcttc                                                                                                                                             <  6:188693/149‑1 (MQ=255)
     ctgcccATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAGCGGTGTTTGGTGGTGGCGAAAATAATCGCTCTGGCTGGCCACTCGTCTTCGATCAGCGTTTGCAGCAAACGCAGTTTTTCTTCGTTAGAAGGGTAGAAAAGTTcttctt                                                                                                                                           <  5:410648/145‑1 (MQ=255)
     aTGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTcttctt                                                                                                                                           <  8:115545/149‑1 (MQ=255)
      tGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTcttctt                                                                                                                                           >  7:80691/1‑148 (MQ=255)
       gACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCttt                                                                                                                                          >  1:391941/1‑148 (MQ=255)
         ccATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTaa                                                                                                                                        >  3:353303/1‑148 (MQ=255)
                ctgcCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGATAAGTTCTTCTTTAATACGGt                                                                                                                                  <  4:119872/147‑1 (MQ=255)
                ctgcCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTgg                                                                                                                                >  8:432493/1‑149 (MQ=255)
                   ccAGGTGGCCCCTGAGCTCTTCACAACGGTGTTTGGGGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGAGCAGCGTTTGCAGCAAACGCTTTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTgggc                                                                                                                              <  6:169097/148‑3 (MQ=255)
                   ccAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTaa                                                                                                                                        <  5:90749/138‑1 (MQ=255)
                   ccAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTAGTTAGAAGGGTAGAAAAGTTCTTCTTTaa                                                                                                                                        >  6:90749/1‑138 (MQ=255)
                     aGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATa                                                                                                                                      <  3:61892/138‑1 (MQ=255)
                     aGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGt                                                                                                                           >  1:168825/1‑149 (MQ=255)
                     aGGTGGCCCCAGATCTCTTCACAACGGTGGTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGT‑GCCCGtt                                                                                                                          <  1:110616/149‑1 (MQ=255)
                        tGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGtttt                                                                                                                        <  4:353303/149‑1 (MQ=255)
                          gCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCt                                                                                                                      >  6:141205/1‑149 (MQ=255)
                            cccAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCttt                                                                                                                                          >  3:45171/1‑127 (MQ=255)
                            cccAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGt                                                                                                                    >  1:353396/1‑149 (MQ=255)
                                    tctTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTc                                                                                                            >  2:274435/1‑149 (MQ=255)
                                     ctTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTc                                                                                                            >  7:263878/1‑148 (MQ=255)
                                      ttCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTcttctt                                                                                                                                           >  7:458969/1‑116 (MQ=255)
                                      ttCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTcttctt                                                                                                                                           <  8:458969/116‑1 (MQ=255)
                                      ttCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCa                                                                                                          >  2:5742/1‑149 (MQ=255)
                                      ttCACAACGGTGTTTGGTGTTGGCGAAAAGAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCa                                                                                                          >  4:338601/1‑149 (MQ=255)
                                      ttCACAACGGTGTTTGGTGTTGGCAAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCa                                                                                                          >  7:322163/1‑149 (MQ=255)
                                        cacaACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCa                                                                                                          <  7:381707/147‑1 (MQ=255)
                                          caACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACtt                                                                                                       >  8:289128/1‑148 (MQ=255)
                                                     ggTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCg                                                                                            <  2:241226/148‑1 (MQ=255)
                                                       tgtTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCa                                                                                         <  3:378234/149‑1 (MQ=255)
                                                       tgtTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCa                                                                                         <  4:328078/149‑1 (MQ=255)
                                                           ggCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGt                                                                                     >  4:44110/1‑149 (MQ=255)
                                                             cGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTc                                                                                   <  2:305872/149‑1 (MQ=255)
                                                              gAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCa                                                                                  >  4:379363/1‑149 (MQ=255)
                                                              gAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCa                                                                                  <  7:432493/149‑1 (MQ=255)
                                                               aaaaTAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCAt                                                                                 >  1:15369/1‑149 (MQ=255)
                                                                aaaTAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATc                                                                                >  6:59045/1‑149 (MQ=255)
                                                                  ataatCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAat                                                                                                   >  4:189777/1‑128 (MQ=255)
                                                                  ataatCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAat                                                                                                   <  3:189777/128‑1 (MQ=255)
                                                                  ataatCGCTCTGGCTGGCCACTCTTCTCCGATCAGCGGTTGCAGCAAACGCATTTTTTCTTCGTTCGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGTTTCCACTTCAATATATTCGGCATTGTTCATCTg                                                                              <  6:150807/149‑1 (MQ=255)
                                                                   taatCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGc                                                                             >  4:278677/1‑149 (MQ=255)
                                                                   taatCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGc                                                                             <  1:204918/149‑1 (MQ=255)
                                                                     atCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTc                                                                           <  5:59045/149‑1 (MQ=255)
                                                                        gCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTc                                                                                                                       <  7:125703/102‑1 (MQ=255)
                                                                         ctctGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAAc                                                                       <  1:329224/149‑1 (MQ=255)
                                                                          tctgtctgGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACg                                                                      >  1:118698/1‑149 (MQ=255)
                                                                              tctgGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGcc                                                                    <  1:274435/147‑1 (MQ=255)
                                                                              tctgGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGc                                                                     <  3:278677/146‑1 (MQ=255)
                                                                                 ggCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGtt                                                                >  4:8296/1‑148 (MQ=255)
                                                                                   ccACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCAc                                                             <  1:5742/149‑1 (MQ=255)
                                                                                    cACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACTGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACg                                                            <  8:417079/149‑1 (MQ=255)
                                                                                    cACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGc                                                                             <  6:395347/132‑1 (MQ=255)
                                                                                    cACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACg                                                            >  8:384137/1‑149 (MQ=255)
                                                                                    cACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACg                                                            >  7:105791/1‑149 (MQ=255)
                                                                                    cACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACg                                                            >  8:27666/1‑149 (MQ=255)
                                                                                     aCTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGaa                                                                        >  8:15351/1‑136 (MQ=255)
                                                                                     aCTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGaa                                                                        <  7:15351/136‑1 (MQ=255)
                                                                                     aCTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGt                                                           <  4:54365/149‑1 (MQ=255)
                                                                                       tcttctTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCaa                                                                                                    >  5:341934/1‑106 (MQ=255)
                                                                                           cttcGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTGCACTTGAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCg                                                      <  2:353396/148‑1 (MQ=255)
                                                                                           cttcGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTAcc                                                        <  2:168825/146‑1 (MQ=255)
                                                                                            ttcGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCAc                                                             <  7:288619/140‑1 (MQ=255)
                                                                                               gATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTc                                                                           <  7:453289/123‑1 (MQ=255)
                                                                                                aTCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCAt                                                                                 >  6:362890/1‑116 (MQ=255)
                                                                                                aTCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACg                                                 >  2:147383/1‑148 (MQ=255)
                                                                                                 tCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGa                                                >  3:21160/1‑148 (MQ=255)
                                                                                                   aGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGaaa                                              <  3:85195/148‑1 (MQ=255)
                                                                                                       tttGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTg                                         >  6:359954/1‑149 (MQ=255)
                                                                                                          gcagcaAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGc                                       <  5:365668/148‑1 (MQ=255)
                                                                                                            agcaAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCg                                      >  8:131544/1‑147 (MQ=255)
                                                                                                            agcaAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGga                                    <  5:359954/149‑1 (MQ=255)
                                                                                                                 aCGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCg                                      >  5:192887/1‑142 (MQ=255)
                                                                                                                 aCGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGaa                                 >  8:49427/1‑147 (MQ=255)
                                                                                                                  cGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAg                              <  3:10288/149‑1 (MQ=255)
                                                                                                                   gCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAg                              >  5:143124/1‑148 (MQ=255)
                                                                                                                   gCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGc                             <  8:112480/149‑1 (MQ=255)
                                                                                                                     aTTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCAt                           >  4:298299/1‑149 (MQ=255)
                                                                                                                      ttttttCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGt                                                           <  6:106490/116‑1 (MQ=255)
                                                                                                                      ttttttCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGt                                                           >  5:106490/1‑116 (MQ=255)
                                                                                                                            cttcGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGat                     <  1:442638/148‑2 (MQ=255)
                                                                                                                            cttcGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAgg                    >  5:168600/1‑149 (MQ=255)
                                                                                                                            cttcGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAg                     <  4:21160/148‑1 (MQ=255)
                                                                                                                             ttcGTTAGAATGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGgc                   >  2:471802/1‑149 (MQ=255)
                                                                                                                             ttcGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGc                                       <  6:413933/129‑1 (MQ=255)
                                                                                                                             ttcGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGc                                       >  5:413933/1‑129 (MQ=255)
                                                                                                                             ttcGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGgc                   >  2:423503/1‑149 (MQ=255)
                                                                                                                                 ttAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTg               >  8:258857/1‑149 (MQ=255)
                                                                                                                                 ttAGAAGGGTAGAAAAGTTCTTCGTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATCTTCGGCCTTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTg               <  3:338601/149‑1 (MQ=255)
                                                                                                                                   aGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGt             >  3:401740/1‑149 (MQ=255)
                                                                                                                                    gAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGc                                       <  6:340920/122‑1 (MQ=255)
                                                                                                                                    gAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGc                                       >  5:340920/1‑122 (MQ=255)
                                                                                                                                    gAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGtt            >  3:274276/1‑149 (MQ=255)
                                                                                                                                        ggTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCa        <  6:210479/149‑1 (MQ=255)
                                                                                                                                          tAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAgg      >  1:361106/1‑149 (MQ=255)
                                                                                                                                            gAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTTACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAggcg    <  2:118698/149‑1 (MQ=255)
                                                                                                                                             aaaaGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGgcgc                 <  1:291234/135‑1 (MQ=255)
                                                                                                                                              aaaGTTCTTCTTTTATACGGTTGCGCTTTTTCTGTTGAGGTTCCTCTTCAATATTTTCTGCATTGTTCATCTGCTCGTACGGCAGTTCACGTTCCCGGTACGAAAGCGTGGCGGAGAATAGCTTGTTGAGGAGCTGGTTTGCAtgcgga  <  2:370983/149‑2 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGC  >  NC_000913/3964783‑3965073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑