Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 289,257 T→A 6.2% intergenic (‑95/+44) yagI ← / ← argF CP4‑6 prophage; putative DNA‑binding transcriptional regulator/ornithine carbamoyltransferase 2, chain F; CP4‑6 prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913289,2570TA 6.2% 238.5 / 5.8 81intergenic (‑95/+44)yagI/argFCP4‑6 prophage; putative DNA‑binding transcriptional regulator/ornithine carbamoyltransferase 2, chain F; CP4‑6 prophage
Reads supporting (aligned to +/- strand):  ref base T (47/27);  new base A (2/3);  total (49/32)
Fisher's exact test for biased strand distribution p-value = 3.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.81e-01

GTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTCGTCCGTCA  >  NC_000913/289109‑289404
                                                                                                                                                    |                                                                                                                                                   
gTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCtgt                                                                                                                                                     >  1:189003/1‑149 (MQ=255)
gTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCggt                                                                                                                                                     >  4:131556/1‑149 (MQ=255)
   gAACAGGTCGAGGATCTGCACCGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTgg                                                                                                                                                   <  8:287219/148‑1 (MQ=255)
   gAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTgg                                                                                                                                                   >  8:192884/1‑148 (MQ=255)
   gAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTgg                                                                                                                                                   <  2:189003/148‑1 (MQ=255)
         gTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCtgtg                                                                                                                                                    <  1:19435/141‑1 (MQ=255)
         gTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCtgtg                                                                                                                                                    >  2:19435/1‑141 (MQ=255)
         gTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAggg                                                                                                                                            >  4:211391/1‑149 (MQ=255)
         gTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCACAGGGAGAggg                                                                                                                                            <  1:205685/149‑1 (MQ=255)
          tCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCACAGGGAGAggga                                                                                                                                           <  2:50280/149‑2 (MQ=255)
          tCGAGGATCTGCAACGCAAGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCAACGCTGGAATTAAGGCGAAAACTTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCACTATCCCTGTGTGAGAggtc                                                                                                                                           >  6:269176/1‑147 (MQ=255)
            gAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCCCGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCg                                                                                                                                         >  2:316283/1‑149 (MQ=255)
            gAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCg                                                                                                                                         >  8:147948/1‑149 (MQ=255)
            gAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCACAGGGAGAggga                                                                                                                                           <  7:428300/147‑2 (MQ=255)
            gAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCACAGGGAGAggga                                                                                                                                           >  8:428300/1‑146 (MQ=255)
             aGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCgg                                                                                                                                        <  8:470500/149‑1 (MQ=255)
              ggATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCggg                                                                                                                                       <  4:149682/149‑1 (MQ=255)
              ggATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCggg                                                                                                                                       >  7:174871/1‑149 (MQ=255)
               taTCTGCAACGCACGTTGAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGTGATGAAAAATCAATTAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCggg                                                                                                                                       <  3:131556/147‑1 (MQ=255)
               gATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCgggg                                                                                                                                      >  7:271978/1‑149 (MQ=255)
                 tCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCACTGTGGGAGAGGGCCGGGGt                                                                                                                                     >  2:98017/1‑148 (MQ=255)
                   tGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg                                                                                                                                  >  5:338133/1‑149 (MQ=255)
                     cAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGa                                                                                                                                   <  3:372878/146‑1 (MQ=255)
                     cAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGa                                                                                                                                   <  8:144027/146‑1 (MQ=255)
                     cAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg                                                                                                                                  <  7:192884/147‑1 (MQ=255)
                     cAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg                                                                                                                                  <  3:234963/147‑1 (MQ=255)
                             gTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAGACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAAcg                                                                                                                        >  3:261006/1‑149 (MQ=255)
                                 aaCAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAAcgcg                                                                                                                      >  3:429289/1‑147 (MQ=255)
                                  aCAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTgg                                                                                                                                                   <  2:318582/117‑1 (MQ=255)
                                  aCAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTgg                                                                                                                                                   >  1:318582/1‑117 (MQ=255)
                                    aGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGagag                                                                                                                                              <  4:338508/120‑1 (MQ=255)
                                    aGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGagag                                                                                                                                              >  3:338508/1‑120 (MQ=255)
                                     gACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCGTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCg                                                                                                                 >  6:171266/1‑148 (MQ=255)
                                          aataatCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACGAACGCGCAGCGGAccc                                                                                                            >  7:238784/1‑148 (MQ=255)
                                          aataatCGGCATAATGCTGGCATGTCCACGCGGCAATTAAGGCCAAACCGTACCTTTTTCGGGATTAAAAATAAATCAAATGGCGCCGGTGTTCGCCCTCTCCCTGTGGGAGAGGGCCGGGGGGAGGGCACCAACGCGCAGCGGAccc                                                                                                            <  6:154488/148‑1 (MQ=38)
                                              atCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCa                                                                                                       >  6:193905/1‑149 (MQ=255)
                                               tCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCaa                                                                                                          >  5:278195/1‑145 (MQ=255)
                                                 ggCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTc                                                                                                    >  6:265892/1‑149 (MQ=255)
                                                 ggCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTc                                                                                                    >  1:310776/1‑149 (MQ=255)
                                                    aTAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTcccc                                                                                                 >  8:203734/1‑149 (MQ=255)
                                                        tGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGc                                                                                             >  4:319243/1‑149 (MQ=255)
                                                               aTGTCCACGCTGTAATTAAGGCTAAAACGTACCTTTTTCGGTATTAAAAATCAATTAAATGGAGCCGGTGTGCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCca                                                                                      <  3:211391/149‑1 (MQ=255)
                                                               aTGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCCGCCCCaa                                                                                                          >  5:74722/1‑129 (MQ=255)
                                                                    cACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTccc                                                                                                  >  1:311230/1‑132 (MQ=255)
                                                                    cACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCatcatc                                                                                 <  3:319243/149‑1 (MQ=255)
                                                                         tGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg                                                                                                                                  <  2:296144/95‑1 (MQ=255)
                                                                         tGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg                                                                                                                                  >  1:296144/1‑95 (MQ=255)
                                                                           taatttcggCGAATCCGTACTTTTTTGGGTATTAAAAATCAATGAATTGGGCCCGGTGTTCCCCCGCTCCTTGTGGGTGTGGGCGGGGGGTTGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCt                                                                          <  4:261006/142‑1 (MQ=255)
                                                                             aTTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAAt                                                                                                         >  3:356741/1‑116 (MQ=255)
                                                                              ttAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTaa                                                                       >  4:371995/1‑149 (MQ=255)
                                                                                 aGGCTAAAACGTAACTTTTTCGTTATGAAAAATAAATTAAATGGAGACGGTGTTCTCCCTCTCCCTGTGGTAGAGGGCGGGGGTTAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCg                                                                    <  1:316283/149‑1 (MQ=255)
                                                                                 aGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCg                                                                    >  5:141062/1‑149 (MQ=255)
                                                                                   gccaaaaCTTACTTTTTTGGGTTTTAAAAATCATTTAATGGGTGCCGGTGTGCGCCCTCTCCGTGGGGTTGTGGGCGGGGGTGTGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTa                                                                  <  4:429289/146‑1 (MQ=255)
                                                                                    cGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTAt                                                                 >  1:310438/1‑149 (MQ=255)
                                                                                    cGAAAACGTACCTTTTGGGGTATTAAAAATAAATTAAATGGAGCGGGTGTGCGCCCTCTCCCTGTGGTTGTGGGCGGGGGGGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTAt                                                                 <  7:147948/149‑1 (MQ=255)
                                                                                        aaCTTACCTTTTGGGGCAAGAAAAATCAATGAATGGTACCGGGTGTCCTCCCTCTCCCTGTGTGAGAGGTCGGGGTTTAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCAt                                                             <  5:175450/149‑1 (MQ=255)
                                                                                          cGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGATCTCCCTCTCACTGTGGGAGAGGGGCGGGGTGAGGGCACCAACGCGCACAGGACCAAAACACTCAACAAGCGTTGCCATCATCACCGCCATAATCGTATGCAAcc                                                           >  5:448982/1‑149 (MQ=255)
                                                                                           gTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGAcc                                                                                                             >  5:317064/1‑98 (MQ=255)
                                                                                            tACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGCCCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCgg                                                         >  3:226606/1‑149 (MQ=255)
                                                                                               cTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGttt                                                      <  5:171266/149‑1 (MQ=255)
                                                                                                       gtattaaaaaTCAATGAAATGGAGCCGGGGTTCTCCCTCGCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTg                                              <  2:310776/144‑1 (MQ=255)
                                                                                                        gATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTgg                                             >  1:172747/1‑149 (MQ=255)
                                                                                                             aaaaTCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGaa                                        >  1:167353/1‑149 (MQ=255)
                                                                                                                   attGAATTGGCGCCGGGGTCCTCCCTCTCCCGGGGGTTGGGGGCCGGGGTTCGGCCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGa                                   <  6:141062/146‑1 (MQ=255)
                                                                                                                   aaTGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGAt                                  >  3:144926/1‑149 (MQ=255)
                                                                                                                    aTGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATg                                 >  1:26035/1‑149 (MQ=255)
                                                                                                                       aaaTGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTc                              >  2:7047/1‑149 (MQ=255)
                                                                                                                           ggAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTcgccg                          >  5:227562/1‑149 (MQ=255)
                                                                                                                               cggtttttCTCAGTCTCCTTGTGGTTGTGGGCGGGGGTTAGGTCACCAACGCGCAGTGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGa                      <  6:382871/143‑1 (MQ=255)
                                                                                                                                cGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCACCGCGCCGCGGACCCAATCACTCCCCAAGCGTTGCCCTCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGAc                     >  5:199782/1‑149 (MQ=255)
                                                                                                                                     ttCTCCCTCTCCTTGTGGGTTTGGGCGGGGGTGAGGGCACTAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCaaa                <  8:238784/149‑1 (MQ=255)
                                                                                                                                     tgctccctccccctGTGGGTGCGGGCGGGGGGGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCaaa                <  6:227562/147‑1 (MQ=255)
                                                                                                                                       ctccctctccctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTcc                                                   >  7:79637/1‑112 (MQ=255)
                                                                                                                                       ctccctctccctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAac               <  3:371995/148‑1 (MQ=255)
                                                                                                                                       ctccctctccctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATAACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAaca              >  3:114081/1‑149 (MQ=255)
                                                                                                                                         ccctctccctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGtt                                                       <  8:18957/106‑1 (MQ=255)
                                                                                                                                         ccctctccctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGtt                                                       >  7:18957/1‑106 (MQ=255)
                                                                                                                                          cctctccctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCt           >  3:233995/1‑149 (MQ=255)
                                                                                                                                          cctctcccaCAGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCt           >  8:97748/1‑149 (MQ=255)
                                                                                                                                               ccctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCCGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTcgtcc      >  5:305839/1‑149 (MQ=255)
                                                                                                                                                cctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCACGCGTTGCCATCATCACCGCCTTAATCGTATGAATCCGGGTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAAGACCTcgtgcg     >  2:373935/1‑149 (MQ=255)
                                                                                                                                                cctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCGTTAATCGTAt                                                                 >  1:59578/1‑89 (MQ=255)
                                                                                                                                                cctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTAt                                                                 <  2:59578/89‑1 (MQ=255)
                                                                                                                                                cctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTcgtccg     >  7:314664/1‑149 (MQ=255)
                                                                                                                                                cctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTcgtccg     >  4:221045/1‑149 (MQ=255)
                                                                                                                                                   gtgGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCATTCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGCTGCTCGCCGCCGACTCAAACACCTCGTCCGTCa  >  6:267076/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTCGTCCGTCA  >  NC_000913/289109‑289404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: