Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,118,828 T→G 6.6% intergenic (+68/+17) zapB → / ← rraA FtsZ stabilizer, septal ring assembly factor, cell division stimulator/ribonuclease E (RNase E) inhibitor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,118,8280TG 6.6% 373.0 / 16.4 122intergenic (+68/+17)zapB/rraAFtsZ stabilizer, septal ring assembly factor, cell division stimulator/ribonuclease E (RNase E) inhibitor protein
Reads supporting (aligned to +/- strand):  ref base T (54/60);  new base G (5/3);  total (59/63)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

Only 100 of 125 total aligned reads displayed.

GAGAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGCCAGCG  >  NC_000913/4118680‑4118974
                                                                                                                                                    |                                                                                                                                                  
gagaACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCt                                                                                                                                                    >  8:235421/1‑149 (MQ=255)
   aacaacCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGc                                                                                                                                                 >  8:112225/1‑149 (MQ=255)
    acaacCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCg                                                                                                                                                <  7:204846/149‑1 (MQ=255)
      aacCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGt                                                                                                                                              >  8:51225/1‑149 (MQ=255)
      aacCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGt                                                                                                                                              <  8:356806/149‑1 (MQ=255)
      aacCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGt                                                                                                                                              >  5:262268/1‑149 (MQ=255)
          aTCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCt                                                                                                                                          >  5:94280/1‑149 (MQ=255)
             tGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTc                                                                                                                                       >  3:356521/1‑149 (MQ=255)
             tGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTCCGGTGCCttta                                                                                                                                       >  5:372034/1‑148 (MQ=255)
              gAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCt                                                                                                                                      >  6:32833/1‑149 (MQ=255)
               aaaGAACAGCAGAACGGCTGGCATGAACGTCTGCAGTCCCTGCTGGGTCGCATGGAAGAGTTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTc                                                                                                                                       <  6:333473/147‑1 (MQ=255)
               aaaGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTc                                                                                                                                       <  8:466966/147‑1 (MQ=255)
               aaaGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTc                                                                                                                                       <  7:112225/147‑1 (MQ=255)
               aaaGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTc                                                                                                                                       <  5:223339/147‑1 (MQ=255)
               aaaGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCtt                                                                                                                                     <  1:130450/149‑1 (MQ=255)
               aaaGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCGCAAGGTGCCtt                                                                                                                                         <  7:392696/145‑1 (MQ=255)
                 aGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCGCAAGGTGCCttttttct                                                                                                                                   <  2:162212/149‑3 (MQ=255)
                   aaCAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCa                                                                                                                                 <  1:342350/149‑1 (MQ=255)
                    aCAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAt                                                                                                                                >  3:144311/1‑149 (MQ=255)
                    aCAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAt                                                                                                                                >  3:402132/1‑149 (MQ=255)
                       tcagAACGGCTGGCAGGAACGTCTGGAGGCCGTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGGCTACAGGTAACGCAGAAAAAAGTCACCTTGCTGTGCCTTTCTTATCATTCa                                                                                                                             <  8:448395/148‑1 (MQ=255)
                        cagAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCaa                                                                                                                            >  7:454221/1‑149 (MQ=255)
                           aaCGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCaa                                                                                                                            <  6:108105/146‑1 (MQ=255)
                           aaCGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCaa                                                                                                                            >  5:108105/1‑146 (MQ=255)
                               gCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAg                                                                                                                     >  4:420202/1‑149 (MQ=255)
                                  ggCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAg                                                                                                                     <  3:100291/146‑1 (MQ=255)
                                  ggCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAg                                                                                                                     >  4:100291/1‑146 (MQ=255)
                                     aGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTCCGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtc                                                                                                               <  1:186898/149‑1 (MQ=255)
                                     aGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtc                                                                                                               >  3:9614/1‑149 (MQ=255)
                                              tGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGa                                                                                                                 <  7:304642/138‑1 (MQ=255)
                                               gCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATACAATATCCAGCGGATCTTCTGATAg                                                                                                      >  2:445598/1‑148 (MQ=255)
                                               gCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGAGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCTATATCCAGCGGATCTTCTGAAAg                                                                                                      >  2:453925/1‑148 (MQ=255)
                                                cAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGaaa                                                                                                       <  4:144311/146‑1 (MQ=255)
                                                cAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGaaa                                                                                                       <  4:402132/146‑1 (MQ=255)
                                                cAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAg                                                                                                      <  2:389287/147‑1 (MQ=255)
                                                 aGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaat                                                                                                   <  6:422661/149‑1 (MQ=255)
                                                   gCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaataa                                                                                                 >  5:351758/1‑149 (MQ=255)
                                                   gCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaataa                                                                                                 >  8:365495/1‑149 (MQ=255)
                                                    cccTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaataat                                                                                                >  5:416091/1‑149 (MQ=255)
                                                     ccTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATc                                                                                               >  3:196029/1‑149 (MQ=255)
                                                      ctgctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAtt                                                                                                                               >  8:306948/1‑116 (MQ=255)
                                                      ctgctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAtt                                                                                                                               <  7:306948/116‑1 (MQ=255)
                                                         ctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCgg                                                                                           >  8:329639/1‑149 (MQ=255)
                                                         ctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCg                                                                                            <  5:254548/148‑1 (MQ=255)
                                                         ctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCg                                                                                            <  6:262268/148‑1 (MQ=255)
                                                         ctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCg                                                                                            <  6:328708/148‑1 (MQ=255)
                                                          tgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCgg                                                                                           >  5:397370/1‑148 (MQ=255)
                                                            ggTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGt                                                                                          <  4:199743/147‑1 (MQ=255)
                                                                gCATGGTAGAGGTCTGATTACGATTCATCTTCCGCGTAATTTCTTTTCTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCAACTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGt                                                                                     <  4:356521/148‑1 (MQ=255)
                                                                  aTGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCgg                                                                                  <  5:32833/149‑1 (MQ=255)
                                                                   tGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGc                                                                                 <  4:215331/149‑1 (MQ=255)
                                                                     gaatcggTCTGATTACGCGTCATCTTCCGCGTAATTTCTTTACTTTGTAGGCCAACAGGTAACGCATAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCa                                                                                <  2:319677/143‑1 (MQ=255)
                                                                           gTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGAt                                                                         <  7:329639/149‑1 (MQ=255)
                                                                             cTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATgg                                                                       >  7:349156/1‑149 (MQ=255)
                                                                                aTTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaataa                                                                                                 <  2:49131/120‑1 (MQ=255)
                                                                                aTTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaataa                                                                                                 >  1:49131/1‑120 (MQ=255)
                                                                                aTTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTc                                                                     <  8:454221/148‑1 (MQ=255)
                                                                                aTTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTc                                                                     <  8:349156/148‑1 (MQ=255)
                                                                                aTTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCg                                                                    >  3:393703/1‑149 (MQ=255)
                                                                                       ttCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGgag                                                              >  5:595/1‑148 (MQ=255)
                                                                                            cTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGaagaag                                                        >  6:230043/1‑149 (MQ=255)
                                                                                               ccGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGaagaag                                                        <  7:365495/146‑1 (MQ=255)
                                                                                               ccGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGaagaag                                                        <  7:462763/146‑1 (MQ=255)
                                                                                               ccGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGaagaag                                                        <  6:94280/146‑1 (MQ=255)
                                                                                               ccGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGt                                                      <  4:429036/148‑1 (MQ=255)
                                                                                                cgcgTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGa                                                    >  1:175990/1‑149 (MQ=255)
                                                                                                 gcgTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTa                                                                                         <  1:300144/111‑1 (MQ=255)
                                                                                                 gcgTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGa                                                    >  8:61253/1‑148 (MQ=255)
                                                                                                   gTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCg                                                                                   >  5:429286/1‑115 (MQ=255)
                                                                                                        ttCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGcc                                             >  6:420438/1‑148 (MQ=255)
                                                                                                        ttCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGcc                                             >  2:98067/1‑148 (MQ=255)
                                                                                                         tCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAACGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCa                                            <  4:143946/148‑1 (MQ=255)
                                                                                                               cTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTg                                      <  6:397370/148‑1 (MQ=255)
                                                                                                                 gtgtAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGAcg                                   >  5:21391/1‑149 (MQ=255)
                                                                                                                    tAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGAcgcg                                 >  2:466668/1‑148 (MQ=255)
                                                                                                                       gCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACa                             >  8:352478/1‑149 (MQ=255)
                                                                                                                         cAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCACGGTATTGTCAGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATc                           >  6:321686/1‑149 (MQ=255)
                                                                                                                            ctggTAACGAAGAAAAAAGGCACCTTGCGGTGGCTTTCTTATCATTCAATATCCTGCGGATCTTCTGAAATAATAATCCCGGTATTGTCGGCTTAAAGATGGTCGCCGGAGAAGAAGGTGAGACCGCCAAAATTGa                                     <  6:210231/134‑1 (MQ=255)
                                                                                                                            cAGGTAACGCAGAAAAAAGGCACCTTGAGGTGCCTTTCTTATCATTCAATATACATCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCTGAGAAGAAGGTGACACCGACAAAATTGa                                     >  5:210231/1‑136 (MQ=255)
                                                                                                                               gTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTc                     >  3:27467/1‑149 (MQ=255)
                                                                                                                               gTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTc                     <  1:409348/149‑1 (MQ=255)
                                                                                                                                  aCGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGc                   >  8:231755/1‑148 (MQ=255)
                                                                                                                                  aCGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGc                   <  2:175990/148‑1 (MQ=255)
                                                                                                                                  aCGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGc                   <  6:416091/148‑1 (MQ=255)
                                                                                                                                    gCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCaa                >  4:296352/1‑149 (MQ=255)
                                                                                                                                     gataagaaaGGCACCGCAAGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAAt               >  8:207786/7‑149 (MQ=255)
                                                                                                                                     cAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAAt               <  3:421641/149‑1 (MQ=255)
                                                                                                                                      aGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATg              <  3:296352/149‑1 (MQ=255)
                                                                                                                                       taagaaaGGCACCGCAAGGTGCCTTTTTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGc             >  8:59534/5‑149 (MQ=255)
                                                                                                                                          gaaaGGCACCGCAAGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCtt          >  1:11487/2‑149 (MQ=255)
                                                                                                                                          aaaaGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGt                                                      <  3:406542/105‑1 (MQ=255)
                                                                                                                                          aaaaGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGt                                                      >  4:406542/1‑105 (MQ=255)
                                                                                                                                           aaaGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGTATAATCCCCGTATTTTCCGCAACAAGATGGTCGCCGGAGAAGAAGGTGCCACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTc         <  6:300754/149‑1 (MQ=255)
                                                                                                                                           aaaGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTc         <  3:420202/149‑1 (MQ=255)
                                                                                                                                           aaaGGCACCGCAAGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTc         <  7:207786/149‑1 (MQ=255)
                                                                                                                                              ggCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGc       >  2:396017/1‑148 (MQ=255)
                                                                                                                                                  ccTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGCCAGCg  <  4:393703/149‑1 (MQ=255)
                                                                                                                                                  ccTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGCCAGCg  <  1:396017/149‑1 (MQ=255)
                                                                                                                                                    ttGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCaa                >  2:363633/1‑133 (MQ=255)
                                                                                                                                                    ttGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCaa                <  1:363633/133‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GAGAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGCCAGCG  >  NC_000913/4118680‑4118974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: