BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,335,655	T→A	5.6%	intergenic (‑65/+39)	eptA ← / ← adiC	lipid A phosphoethanolamine transferase/arginine:agmatine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,335,655	0	T	A	5.6%	814.7 / 21.0	245	intergenic (‑65/+39)	eptA/adiC	lipid A phosphoethanolamine transferase/arginine:agmatine antiporter
Reads supporting (aligned to +/- strand): ref base T (107/124); new base A (3/11); total (110/135)
Fisher's exact test for biased strand distribution p-value = 9.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

AGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCA  >  NC_000913/4335508‑4335798

aGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTc                                                                                                                                                   >  6:10374/1‑149 (MQ=255)

aGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGAGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTc                                                                                                                                                   >  4:225628/1‑149 (MQ=255)

   tggtcatttgtcaccctgatggtcatcaccgccatgtatgcgctgaattacaaccggctacataaaaacccgtatcccttagatgcaccaataagcaaagattaattccccttaatccagcaaacataaAAGCCAACCTTAAGTTCtt                                                                                                                                                 <  8:134984‑M1/19‑1 (MQ=255)

   cAGATAGAGATATTAAAAGCGGCCAACAATATCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTa                                                                                                                                                <  8:376713/149‑1 (MQ=255)

   cAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTa                                                                                                                                                <  7:135700/149‑1 (MQ=255)

     gATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAg                                                                                                                                              <  5:400862/149‑1 (MQ=255)

     gATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAg                                                                                                                                              >  2:138499/1‑149 (MQ=255)

      aTAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAgg                                                                                                                                             <  3:23236/149‑1 (MQ=255)

      aTAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAAATCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAgg                                                                                                                                             >  4:371310/1‑149 (MQ=255)

         gagaTATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAATGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACATAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAATGTTg                                                                                                                                          <  5:249627/149‑1 (MQ=255)

         gagaTATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGAACTTAAGGTTg                                                                                                                                          <  2:312429/149‑1 (MQ=255)

          agaTATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGGCGTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGAACTTAAGGTTgg                                                                                                                                         <  5:238195/149‑1 (MQ=255)

           gaTATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTATTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGAACTTAAGGTTGGc                                                                                                                                        <  1:103564/149‑1 (MQ=255)

             tataAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTg                                                                                                                                          >  6:342390/1‑145 (MQ=255)

              ataAATAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCtt‑‑t                                                                                                                                   >  6:124574/1‑149 (MQ=255)

                 aaaaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTAt                                                                                                                                <  6:247188/149‑1 (MQ=255)

                  aaaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATg                                                                                                                               >  7:318496/1‑149 (MQ=255)

                        gCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGtt                                                                                                                                           >  6:318113/1‑133 (MQ=255)

                        gCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGtt                                                                                                                                           >  4:282513/1‑133 (MQ=255)

                        gCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGtt                                                                                                                                           <  5:318113/133‑1 (MQ=255)

                           aacaaTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGa                                                                                                                     >  7:33594/1‑149 (MQ=255)

                           aacaaTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGa                                                                                                                     <  5:124574/149‑1 (MQ=255)

                           aacaaTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGATGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGa                                                                                                                     <  1:174630/149‑1 (MQ=255)

                            acaaTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGAt                                                                                                                    <  7:64130/149‑1 (MQ=255)

                             caaTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGAt                                                                                                                    >  3:31956/1‑148 (MQ=255)

                              aaTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGAtt                                                                                                                   >  5:346342/1‑148 (MQ=255)

                                tAGCCAGGCGAGTGAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAg                                                                                                                <  1:65919/149‑1 (MQ=255)

                                tAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAg                                                                                                                <  7:97366/149‑1 (MQ=255)

                                  gCCAGGCGAGTAAATTCAAAGATGGTCTTTTTAGTTGTGGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAggg                                                                                                              <  6:189467/149‑1 (MQ=255)

                                     aGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTgg                                                                                                                                         <  7:347152/122‑1 (MQ=255)

                                     aGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGa                                                                                                                     <  8:345445/139‑1 (MQ=255)

                                     aGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAg                                                                                                                >  1:131472/1‑144 (MQ=255)

                                     aGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGaa                                                                                                           >  1:297264/1‑149 (MQ=255)

                                     aGGCGAGTAAAATCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGaa                                                                                                           <  3:371310/149‑1 (MQ=255)

                                      ggCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAAt                                                                                                          <  4:150310/149‑1 (MQ=255)

                                      ggCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAAt                                                                                                          <  5:15697/149‑1 (MQ=255)

                                          aGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTaa                                                                                                                 <  1:458085/138‑1 (MQ=255)

                                              aaTTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCttt                                                                                                  <  6:242851/149‑1 (MQ=255)

                                               aTTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTg                                                                                                 >  2:432400/1‑149 (MQ=255)

                                                ttCAACGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGTGAATTAATCTTTGc                                                                                                <  1:146250/149‑1 (MQ=255)

                                                   aaaGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑ttt                                                                                                                           <  3:12746/119‑1 (MQ=255)

                                                      gagGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTAtta                                                                                          <  2:459570/149‑2 (MQ=255)

                                                      gagGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTg                                                                                          <  4:195657/149‑1 (MQ=255)

                                                          gTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTAt                                                                                                                                <  7:106839/108‑1 (MQ=255)

                                                             tttttAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGa                                                                                                            >  7:91265/1‑124 (MQ=255)

                                                             tttttAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGa                                                                                                            <  8:91265/124‑1 (MQ=255)

                                                             tttttAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATc                                                                                   >  8:320317/1‑149 (MQ=255)

                                                                  agtagGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATc                                                                                   <  1:91177/144‑1 (MQ=255)

                                                                      ggCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATa                                                                          >  5:68251/1‑149 (MQ=255)

                                                                        cgcTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACg                                                                        <  3:285487/149‑1 (MQ=255)

                                                                          cTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACgg                                                                       >  5:265330/1‑148 (MQ=255)

                                                                           ttCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGt                                                                     >  6:391445/1‑149 (MQ=255)

                                                                             cAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGt                                                                     <  4:137046/147‑1 (MQ=255)

                                                                               aCATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGc                                                                                                <  3:160741/118‑1 (MQ=255)

                                                                               aCATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTg                                                                                          >  3:89400/1‑124 (MQ=255)

                                                                               aCATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGttttt                                                                 <  4:271908/149‑1 (MQ=255)

                                                                               aCATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑ATTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGtttt                                                                  >  1:425540/1‑148 (MQ=255)

                                                                                 aTCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCttt                                                                                                  <  8:118263/114‑1 (MQ=255)

                                                                                        tgtTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGtttt                                                                  >  8:395068/1‑139 (MQ=255)

                                                                                         gtTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCg                                                        <  1:350587/148‑1 (MQ=255)

                                                                                         gtTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCg                                                        >  2:350587/1‑148 (MQ=255)

                                                                                           caataagcaaagattaattccccttaatccagcaaacataaAAGCCAACCTTAAGAACTTAAGGTTGGCTT‑‑GTATG‑TTTTCTGGATTAAGGGGAATTAATCTTGGCTGATTTGTGCATCTAAGGGATAAGGGTTTTTAggtagcaggga                                                     <  6:301579‑M1/108‑6 (MQ=255)

                                                                                              cATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGt                                                   <  4:120568/148‑1 (MQ=255)

                                                                                              cATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGt                                                   >  3:115072/1‑148 (MQ=255)

                                                                                               aTCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAg                                                                                                                                              >  8:23204/1‑59 (MQ=255)

                                                                                               aTCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAAt                                                                                                          >  5:338284/1‑92 (MQ=255)

                                                                                               aTCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCttt                                                                                                  >  5:32256/1‑100 (MQ=255)

                                                                                               aTCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTaa                                                 >  7:118515/1‑149 (MQ=255)

                                                                                                   aaagattaattccccttaatccagcaaacataaAAGCCAACCTTAAGAACTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGtt                                                     <  1:205979‑M1/108‑1 (MQ=255)

                                                                                                   aaCAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTaa                                                 <  1:371671/145‑1 (MQ=255)

                                                                                                   aaCAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAAtt                                               >  8:7649/1‑147 (MQ=255)

                                                                                                   aaCAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAAtt                                               >  7:199664/1‑147 (MQ=255)

                                                                                                    aCAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTg                                                                                                                       >  2:198947/1‑74 (MQ=255)

                                                                                                      aaaGTGCGCATATGCTCGCTAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTG‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAgcg                                          >  7:367164/1‑149 (MQ=255)

                                                                                                      aaaGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTAtt                                                                                           >  1:324649/1‑100 (MQ=255)

                                                                                                      aaaGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTAtt                                                                                           <  2:324649/100‑1 (MQ=255)

                                                                                                          tGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATa                                      >  5:438208/1‑149 (MQ=255)

                                                                                                               atatGCTAGCAAAGCAAAATTAATCCTAACTTATGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGTTTATTGGTGCATGTAAGGGATACGGGTTTTTATTTAGCGGGTTGTAATTCAGCGCATACATgg                                 <  6:334116/149‑1 (MQ=255)

                                                                                                                 atGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTAtt                                                                                           <  2:394139/89‑1 (MQ=255)

                                                                                                                   gCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGt                             >  2:21143/1‑149 (MQ=255)

                                                                                                                     tCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATGAAGGGGAATTAATCTTAGCTTATTGGTGCATCTAAGGGATACGGGGTTTTATGTAGCCGGTTGAAATTCAGCGCATACAGGGCGGt                             >  6:17320/1‑147 (MQ=255)

                                                                                                                      tccagcaaacataaAAGCCAACCTTAAGAACTTAAGGTTGGCTTAATTTTGCTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGt                             <  4:97347‑M1/135‑1 (MQ=255)

                                                                                                                       gcaaagcaaaATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGttttt                                                                 >  7:241890/1‑109 (MQ=255)

                                                                                                                       gcaaagcaaaATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGtgatg                         >  8:352422/1‑149 (MQ=255)

                                                                                                                        caaagcaaaATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGtgatga                        <  4:31956/149‑1 (MQ=255)

                                                                                                                            gcaaaATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCa                     >  6:59920/1‑148 (MQ=255)

                                                                                                                            gcaaaATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCAt                    >  5:246058/1‑149 (MQ=255)

                                                                                                                                aaTTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAgg                <  6:419577/149‑1 (MQ=255)

                                                                                                                                aaTTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCAGCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAgg                >  8:202085/1‑149 (MQ=255)

                                                                                                                                 aTTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGAc                       >  6:442802/1‑141 (MQ=255)

                                                                                                                                      GCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGttttt                                                                 >  5:454656‑M1/1‑94 (MQ=255)

                                                                                                                                        CAACCTTAAGAACTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAggg               >  7:277732‑M1/1‑142 (MQ=255)

                                                                                                                                        CAACCTTAAGAACTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAggg               <  8:277732‑M1/142‑1 (MQ=255)

                                                                                                                                          ACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCg                               >  3:425121‑M1/1‑124 (MQ=255)

                                                                                                                                          ACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCg                               <  4:425121‑M1/124‑1 (MQ=255)

                                                                                                                                           CCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTa                                                                >  2:78423‑M1/1‑90 (MQ=255)

                                                                                                                                             TTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAAt       <  4:64501‑M1/145‑1 (MQ=255)

                                                                                                                                               AAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACca  <  7:414482‑M1/148‑1 (MQ=255)

                                                                                                                                                  ttCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCgg                              <  1:409534/117‑1 (MQ=255)

                                                                                                                                                  ttCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCgg                              >  2:409534/1‑117 (MQ=255)

AGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTT‑‑TTATG‑TTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCA  >  NC_000913/4335508‑4335798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`