Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,338,130 G→C 6.9% intergenic (‑201/+124) adiY ← / ← adiA adi system transcriptional activator/arginine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,338,1300GC 6.9% 729.0 / 14.2 256intergenic (‑201/+124)adiY/adiAadi system transcriptional activator/arginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (113/122);  new base C (2/17);  total (116/140)
Fisher's exact test for biased strand distribution p-value = 1.36e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 264 total aligned reads displayed.

TACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGTTACGCTTTCACGCACATAACGTG  >  NC_000913/4337982‑4338277
                                                                                                                                                    |                                                                                                                                                   
  ccGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGcc                                                                                                                                                   >  8:56243/1‑149 (MQ=255)
          tGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCaaa                                                                                                                                            >  2:454127/1‑148 (MQ=255)
            gTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCaaaaa                                                                                                                                          <  6:264792/148‑1 (MQ=255)
            gTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCaaaaa                                                                                                                                          <  8:28079/148‑1 (MQ=255)
               atataATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTa                                                                                                                                       <  6:412840/148‑1 (MQ=255)
                tataATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTAt                                                                                                                                      >  4:60783/1‑148 (MQ=255)
                tataATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGCCCGCGCAAAAAGTAt                                                                                                                                      <  5:445472/148‑1 (MQ=255)
                tataATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGCCCGCGCAAAAAGTAt                                                                                                                                      <  1:409379/148‑1 (MQ=255)
                   aaTTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACa                                                                                                                                  >  5:132987/1‑149 (MQ=255)
                   aaTTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACa                                                                                                                                  >  3:322233/1‑149 (MQ=255)
                   aaTTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACa                                                                                                                                  >  6:6590/1‑149 (MQ=255)
                        tATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGCCCGCGCAAAAAGTATGACAttttt                                                                                                                             <  2:465831/149‑1 (MQ=255)
                         atgatgAATATAAACATGCATAAAAATAACGAGTTTTACTAATATTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGCATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACAttttt                                                                                                                             <  7:143746/148‑1 (MQ=255)
                           gatgaATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTtttgtt                                                                                                                          >  7:240640/1‑149 (MQ=255)
                            atgaATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTtttgttt                                                                                                                         >  6:424364/1‑149 (MQ=255)
                            atgaATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTtttgtt                                                                                                                          <  2:364936/148‑1 (MQ=255)
                             tgaATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTtttgtttg                                                                                                                        >  1:302930/1‑149 (MQ=255)
                               aaTATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGTATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTgc                                                                                                                       <  3:237084/148‑1 (MQ=255)
                                atatAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTgca                                                                                                                      <  1:454127/148‑1 (MQ=255)
                                 tataAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATATTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGAGCGCGGGGGGTAAGCGCAAAACGTATTACATGTTTCTTTtcag                                                                                                                     >  6:25267/1‑148 (MQ=255)
                                        aTGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAAtttt                                                                                                              >  5:294556/1‑148 (MQ=255)
                                         tGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTa                                                                                                            <  8:449639/149‑1 (MQ=255)
                                           cATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTAt                                                                                                           <  6:320666/148‑1 (MQ=255)
                                             tAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCCTGTACATATTTTTTTACAGGATAAATAACCGGCAAAAAAAAGCCCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGaa                                                                                                        <  5:116029/149‑1 (MQ=255)
                                             tAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAAtttt                                                                                                              <  7:387099/143‑1 (MQ=255)
                                             tAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAAtttt                                                                                                              >  8:387099/1‑143 (MQ=255)
                                              aaaaaTAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGaa                                                                                                        >  6:129974/1‑148 (MQ=255)
                                               aaaaTAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGaaaa                                                                                                      >  2:136689/1‑149 (MQ=255)
                                                aaaTAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTgcag                                                                                                                     >  2:328072/1‑133 (MQ=255)
                                                   tAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTAAAGGATAAATAACAGGAAAAAAAAGCGCGGGGGGGGCCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTg                                                                                                 <  5:352215/149‑1 (MQ=255)
                                                   atattatttttACTTAAAGTTAAAATTTTTTTTTTATTTTTTATTTTTCATTTTTTTTTACAGTATAAAAAACATTCAAAAAAATGCGCGGGTTGGCCTCCGGCAAATAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGtt                                                                                                  <  8:302303/142‑1 (MQ=255)
                                                    aaCGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAACAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTCCAGCAATTTTTATGAAAACGTTg                                                                                                 <  2:298674/149‑1 (MQ=255)
                                                    aaCGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTg                                                                                                 <  7:438559/149‑1 (MQ=255)
                                                     aCGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTg                                                                                                 <  3:108957/148‑1 (MQ=255)
                                                     aCGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGc                                                                                                <  1:175024/149‑1 (MQ=255)
                                                      cGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACAGTTTTGTTTGCAGCAATTTTTATGAAAACGTTGc                                                                                                >  6:148753/1‑148 (MQ=255)
                                                       gAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCa                                                                                              >  8:109124/1‑149 (MQ=255)
                                                          ttttACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCTCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATAc                                                                                           <  4:310597/149‑1 (MQ=255)
                                                               cTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGc                                                                                                <  8:172791/139‑1 (MQ=255)
                                                                tAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACt                                                                                          <  1:322591/144‑1 (MQ=255)
                                                                tAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCaa                                                                                       >  4:425576/1‑147 (MQ=255)
                                                                  aTAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCaaaa                                                                                     <  4:203085/147‑1 (MQ=255)
                                                                  aTAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAgg                                                                                   >  4:336243/1‑149 (MQ=255)
                                                                  aTAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAgg                                                                                   >  1:164966/1‑149 (MQ=255)
                                                                  aTAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAg                                                                                    <  1:396777/148‑1 (MQ=255)
                                                                   tAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAATGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGc                                                                                  <  2:351944/149‑1 (MQ=255)
                                                                   tAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGc                                                                                  <  8:257494/149‑1 (MQ=255)
                                                                   tAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGc                                                                                  <  5:109889/149‑1 (MQ=255)
                                                                   tAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGc                                                                                  <  3:99361/149‑1 (MQ=255)
                                                                     gTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGcc                                                                                 <  1:62896/148‑1 (MQ=255)
                                                                      tACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCt                                                                                >  3:8816/1‑148 (MQ=255)
                                                                      tACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGAGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCt                                                                                >  2:317267/1‑148 (MQ=255)
                                                                        cAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAg                                                                             <  1:100685/149‑1 (MQ=255)
                                                                        cAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAg                                                                             >  4:112373/1‑149 (MQ=255)
                                                                        aaaaTTTTTATAGTAATTTCTCATTTAAATTTTTTTTTACAGTATAAATAACAGGAAAAAAAAAGCGCGGGGTGGGCCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAg                                                                             <  7:300394/148‑1 (MQ=255)
                                                                            tttttATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCa                                                                         <  7:149337/149‑1 (MQ=255)
                                                                              tttATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAgg                                                                       >  4:20254/1‑149 (MQ=255)
                                                                                 ataGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAggcgg                                                                    >  2:38161/1‑149 (MQ=255)
                                                                                  taGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAggcggc                                                                   >  3:218191/1‑149 (MQ=255)
                                                                                  taGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAggcggc                                                                   <  8:240640/149‑1 (MQ=255)
                                                                                  aaGTAATTTGTAATGTAAATATTTTTTAACAGTAAAAAAAACAGGCAAAAAAAAGCGGGGGTGGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAggcgg                                                                    <  7:56243/147‑1 (MQ=255)
                                                                                    gTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGgcggcg                                                                  >  1:95767/1‑148 (MQ=255)
                                                                                            tcATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTc                                                                                         <  1:116945/117‑1 (MQ=255)
                                                                                              aTGTACATATTTTTTTAAAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTAt                                                         <  5:424364/147‑1 (MQ=255)
                                                                                              aTGTAAATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTAtt                                                        <  6:143223/148‑1 (MQ=255)
                                                                                               tGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCCGGGTGGGGCCGCGCAAAAAGTATGAGATATTAGTTTGCAGCAATTTTTa                                                                                                            >  5:173367/1‑95 (MQ=255)
                                                                                                 tACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTaaa                                                    >  6:5089/1‑149 (MQ=255)
                                                                                                 tACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCCGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTACTTaaa                                                    <  8:441534/149‑1 (MQ=255)
                                                                                                 tACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTaaa                                                    <  5:91516/148‑1 (MQ=255)
                                                                                                  aaatatTTTTATACAGGATAAATAACACGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTaaa                                                    <  8:194539/146‑1 (MQ=255)
                                                                                                  aCATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTaaa                                                    >  5:236093/1‑148 (MQ=255)
                                                                                                  aCATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTaaa                                                    >  2:58727/1‑148 (MQ=255)
                                                                                                    atatTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAAc                                                   <  1:440778/147‑1 (MQ=255)
                                                                                                     tatTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAAt                                                <  1:364077/149‑1 (MQ=255)
                                                                                                     tatTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTGTTTGCAGCAATTTTTATGAAAAAGTTGCCATACTCaaaat                                                                                    >  6:109270/1‑111 (MQ=255)
                                                                                                      atTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCATAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAAt                                                >  1:46851/1‑148 (MQ=255)
                                                                                                              aCAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGAAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTGCGCCtt                                        >  7:259440/1‑148 (MQ=255)
                                                                                                               cAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTc                                       <  2:427181/148‑1 (MQ=255)
                                                                                                                aGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAg                                     >  2:103/1‑149 (MQ=255)
                                                                                                                 ggATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGc                                    >  7:186090/1‑149 (MQ=255)
                                                                                                                  gATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTaa                                                                              >  4:406439/1‑106 (MQ=255)
                                                                                                                  gATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTaa                                                                              <  3:406439/106‑1 (MQ=255)
                                                                                                                  gATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCg                                   <  3:60783/149‑1 (MQ=255)
                                                                                                                       ataaCAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAg                             >  7:271562/1‑149 (MQ=255)
                                                                                                                        taaCAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAg                             <  3:425576/149‑1 (MQ=255)
                                                                                                                         aaCAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGt                            >  2:250879/1‑149 (MQ=255)
                                                                                                                          tcAGGCAAAAAAAAGCGCGGGGTGGGCCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTg                           <  5:419424/148‑1 (MQ=255)
                                                                                                                          aCAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGc                                    >  6:222320/1‑140 (MQ=255)
                                                                                                                          aCAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTg                           <  1:250879/149‑1 (MQ=255)
                                                                                                                            aGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGt                         <  2:164966/149‑1 (MQ=255)
                                                                                                                               cAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGTTAc                      <  4:8816/149‑1 (MQ=255)
                                                                                                                                aaaaaaaaGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGt                         >  6:37868/1‑145 (MQ=255)
                                                                                                                                aaaaaaaaGCGCGGGGGGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGt                         <  5:37868/145‑1 (MQ=255)
                                                                                                                                   aaaaaGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGAAATAGTGGTTACGCtt                  >  2:177619/1‑149 (MQ=255)
                                                                                                                                     aaaGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGTTACGCTTTc                <  7:21528/149‑1 (MQ=255)
                                                                                                                                       aGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGTTACGCTTTCAc              <  8:270337/149‑1 (MQ=255)
                                                                                                                                        gcgcGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAACGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGTTACGCTTTCACg             >  3:178507/1‑149 (MQ=255)
                                                                                                                                        gcgcGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGTTACGCTTTCACg             >  5:160799/1‑149 (MQ=255)
                                                                                                                                                   ggCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGTTACGCTTTCACGCACATAACGTg  >  4:249383/1‑149 (MQ=255)
                                                                                                                                                    gCCGCGCAAAAAGTATGAAATTTTTTTTTGCAGCAATTTTTATGACAACTTTGACATAATCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGTTTACGCTTTCACGAACATAACGTg  <  5:25267/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGTTACGCTTTCACGCACATAACGTG  >  NC_000913/4337982‑4338277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: