Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,560,590 A→G 5.2% F31L (TTT→CTT)  yjiH ← nucleoside recognition pore and gate family putative inner membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,560,5900AG 5.2% 244.6 / 2.1 77F31L (TTT→CTT) yjiHnucleoside recognition pore and gate family putative inner membrane transporter
Reads supporting (aligned to +/- strand):  ref base A (39/34);  new base G (2/2);  total (41/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTG  >  NC_000913/4560442‑4560736
                                                                                                                                                    |                                                                                                                                                  
aaGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGaaaaaaa                                                                                                                                                    <  5:395708/149‑1 (MQ=255)
 aGCCATCTTTATACCCCGCGCCACCCGCGGCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAg                                                                                                                                                   <  6:224877/149‑1 (MQ=255)
  gCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGa                                                                                                                                                  <  6:144999/149‑1 (MQ=255)
        tttAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAGGATTATgg                                                                                                                                            >  3:118782/1‑149 (MQ=255)
        tttAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAGGATTATgg                                                                                                                                            >  3:112949/1‑149 (MQ=255)
         ttAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTAt                                                                                                                                              >  4:409965/1‑146 (MQ=255)
         ttAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTAt                                                                                                                                              <  3:409965/146‑1 (MQ=255)
          tAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGt                                                                                                                                           <  4:335851/148‑1 (MQ=255)
          tAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCGGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGt                                                                                                                                           <  2:49290/148‑1 (MQ=255)
            gCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTga                                                                                                                                         <  8:213869/148‑1 (MQ=255)
             cccccGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGATGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTgac                                                                                                                                        >  4:412199/1‑147 (MQ=255)
                                gcgAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCtt                                                                                                                    <  1:194340/149‑1 (MQ=255)
                                gcgAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCtt                                                                                                                    <  8:172823/149‑1 (MQ=255)
                                    aaTGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATTGTGAGAAAAAAGGCCAGATACCCTTTAAt                                                                                                                <  2:328159/149‑1 (MQ=255)
                                    aaTGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAAt                                                                                                                <  3:143914/149‑1 (MQ=255)
                                          gtggtgGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCAc                                                                                                          <  6:188374/149‑1 (MQ=255)
                                          gtggtgGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCAc                                                                                                          >  4:62019/1‑149 (MQ=255)
                                             gtggCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGAt                                                                                                                                                 >  1:284235/1‑107 (MQ=255)
                                             gtggCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGAt                                                                                                                                                 <  2:284235/107‑1 (MQ=255)
                                                  tCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTg                                                                                                  >  2:64167/1‑149 (MQ=255)
                                                  tCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTg                                                                                                  >  6:236530/1‑149 (MQ=255)
                                                  tCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGGCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGCAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTg                                                                                                  >  7:325125/1‑149 (MQ=255)
                                                  tCCGTTCGCACCAGGCAGTTGCAAAAACGATCCGTTCAGAACGGAAAAATCAAAGACGCGCCACCAGCTGTCTGTTCCTGAAAAAAAACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATAACCTTTAATGACCACTTTATCTg                                                                                                  >  5:58178/1‑149 (MQ=255)
                                                    cGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTc                                                                                                <  4:10289/149‑1 (MQ=255)
                                                      ttCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGc                                                                                              >  3:360668/1‑149 (MQ=255)
                                                      tacGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAACGGCCAGATAcc                                                                                                                       <  4:41594/122‑1 (MQ=255)
                                                         gCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACTGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCa                                                                                            <  7:197360/148‑1 (MQ=255)
                                                         gCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAg                                                                                           >  7:277533/1‑149 (MQ=255)
                                                          cACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATAccc                                                                                                                      >  2:313065/1‑121 (MQ=255)
                                                          cACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATAAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATAccc                                                                                                                      <  1:313065/121‑1 (MQ=255)
                                                           aCCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAGGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCt                                                                                         >  4:185282/1‑149 (MQ=255)
                                                           aCCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACtttt                                                                                                      >  7:108965/1‑136 (MQ=255)
                                                           aCCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACtttt                                                                                                      <  8:108965/136‑1 (MQ=255)
                                                            ccAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGaaaaaaa                                                                                                                                                    <  4:55617/89‑1 (MQ=255)
                                                            ccAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGaaaaaaa                                                                                                                                                    >  3:55617/1‑89 (MQ=255)
                                                             cAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTcc                                                                                       >  7:47336/1‑149 (MQ=255)
                                                                        ccAGACGATCCGTTCAGAACGGAAAAATCAAAAACGCGACCCCATCTGTCTGTGCCGGAACAAACACCGGAAAACAAGATTATGGTGAGAAACACGGCAAGCTACACGTGAAGGCCCACTTGGTCTGTCGCCAGCTCCCCGGCTAccg                                                                             >  8:344118/1‑146 (MQ=255)
                                                                         cAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCa                                                                           >  3:202960/1‑149 (MQ=255)
                                                                          aaaCGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGc                                                                                              <  7:432504/129‑1 (MQ=255)
                                                                          aaaCGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGc                                                                                              >  8:432504/1‑129 (MQ=255)
                                                                          aaaCGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCAt                                                                          >  5:432994/1‑149 (MQ=255)
                                                                          aaaCGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCAt                                                                          >  4:125058/1‑149 (MQ=255)
                                                                                tCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTga                                                                                                                                         >  3:246386/1‑80 (MQ=255)
                                                                                tCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTga                                                                                                                                         <  4:246386/80‑1 (MQ=255)
                                                                                  cGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCttg                                                                   <  3:62019/148‑1 (MQ=255)
                                                                                   gTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCttgtt                                                                 <  1:13860/149‑1 (MQ=255)
                                                                                   gTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCttgtt                                                                 <  8:325125/149‑1 (MQ=255)
                                                                                       aGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTgtgt                                                             >  5:362622/1‑149 (MQ=255)
                                                                                        gAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTgtgt                                                             >  8:468260/1‑148 (MQ=255)
                                                                                          aCGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCCGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGACAACTTTTTCTGTCGCCAGCTCCCCCGCTACAGCATCCCCTTGTTGTGTCa                                                           >  4:250240/1‑148 (MQ=255)
                                                                                                   tCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATccc                                                 >  6:419365/1‑149 (MQ=255)
                                                                                                   tCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAAAGCCCACTATTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGATGTGTCATAACAATccc                                                 >  3:349423/1‑149 (MQ=255)
                                                                                                   tCAAAAACGCGCCACAAGCTGTCTGTTCCGGAAAAAACACAGGAAAAAAAGAATATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCAAATTTTTCTGTCGCCCGCTCCCCGCCTAACGCATCCCCTAGTTGTGTCATAACAATccc                                                 >  7:209587/1‑149 (MQ=255)
                                                                                                    cAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATccc                                                 >  3:425133/1‑148 (MQ=255)
                                                                                                     aaaaaCGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATa                                                         <  1:321138/139‑1 (MQ=255)
                                                                                                     aaaaaCGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATa                                                         <  7:136514/139‑1 (MQ=255)
                                                                                                     aaaaaCGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATa                                                         >  8:136514/1‑139 (MQ=255)
                                                                                                     aaaaaCGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATa                                                         >  2:321138/1‑139 (MQ=255)
                                                                                                     aaaaaCGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCa                                               >  4:204346/1‑149 (MQ=255)
                                                                                                       aaaCGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCAtt                                             <  8:277533/149‑1 (MQ=255)
                                                                                                             gcCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTtgttgt                                                               >  1:352606/1‑125 (MQ=255)
                                                                                                             gcCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTtgttgt                                                               <  2:352606/125‑1 (MQ=255)
                                                                                                             gcCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGttttt                                       <  6:362622/149‑1 (MQ=255)
                                                                                                                 ccaGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTtttgt                                   >  1:287081/1‑149 (MQ=255)
                                                                                                                 ccaGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTtttgt                                   <  7:257820/149‑1 (MQ=255)
                                                                                                                 ccaGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTtttgt                                   <  1:64167/149‑1 (MQ=255)
                                                                                                                     ctgtctgtTCCGGAAAAAACACCGGAAAAAAGGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTtttgtgttt                               <  4:118782/149‑1 (MQ=255)
                                                                                                                     ctgtctgtTCCGGAAAAAACACCGGAAAAAAGGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTATCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTtttgtgttt                               <  4:112949/149‑1 (MQ=255)
                                                                                                                     ctgtctgtTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTtttgtgttt                               >  4:86398/1‑149 (MQ=255)
                                                                                                                        tctgtTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCtt                                                                                                                    >  5:229201/1‑61 (MQ=255)
                                                                                                                        tctgtTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCtt                                                                                                                    <  6:229201/61‑1 (MQ=255)
                                                                                                                          tgtTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTtgtgtg                          >  3:104523/1‑149 (MQ=255)
                                                                                                                           gtTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTgtgtgt                         >  7:471142/1‑149 (MQ=255)
                                                                                                                               cGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGc                      <  8:47336/148‑1 (MQ=255)
                                                                                                                                ggAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCtt                    >  7:29640/1‑149 (MQ=255)
                                                                                                                                ggAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTGTTTTTGTGTGTGTGTGTTGCtt                    >  3:270146/1‑149 (MQ=255)
                                                                                                                                ggAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACACTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCtt                    >  4:26614/1‑149 (MQ=255)
                                                                                                                                  aaaaaaCACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGa                  <  4:202960/149‑1 (MQ=255)
                                                                                                                                         acCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGa           <  5:236530/149‑1 (MQ=255)
                                                                                                                                         acCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGa           <  3:86398/149‑1 (MQ=255)
                                                                                                                                                 aaaaGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCt   <  4:360668/149‑1 (MQ=255)
                                                                                                                                                 aaaaGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCt   <  3:204346/149‑1 (MQ=255)
                                                                                                                                                  aaaGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTg  <  6:432994/149‑1 (MQ=255)
                                                                                                                                                  aaaGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTg  >  8:50337/1‑149 (MQ=255)
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AAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAAAGGCCAGATACCCTTTAATGCCCACTTTTTCTGTCGCCAGCTCCCCGGCTACAGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTG  >  NC_000913/4560442‑4560736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑