Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 383,897 C→T 5.4% M13I (ATG→ATA) ‡ yaiP ← putative family 2 glycosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913383,8970CT 5.4% 298.8 / 4.2 92M13I (ATG→ATA) ‡yaiPputative family 2 glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (46/41);  new base T (3/2);  total (49/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

ATTACCCGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCA  >  NC_000913/383751‑384042
                                                                                                                                                  |                                                                                                                                                 
aTTACCCGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACAt                                                                                                                                                 >  4:20637/1‑149 (MQ=255)
   aCCCGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTg                                                                                                                                               <  2:400680/148‑1 (MQ=255)
   aCCCGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGc                                                                                                                                              >  8:218137/1‑149 (MQ=255)
     ccGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTa                                                                                                                                            >  6:258682/1‑149 (MQ=255)
      cGGCAAAATTAAGGGTTACGCAGTAGATTATCCAGTGCCTGCGCCAGACACGGGCGTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACTTCTTAGCGTACTTAAAAACCATAGCAACATTGCTAt                                                                                                                                           <  4:221263/149‑1 (MQ=255)
       ggCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCTTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATg                                                                                                                                          <  4:22683/149‑1 (MQ=255)
        gCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATgg                                                                                                                                         <  5:244319/149‑1 (MQ=255)
            aaaTAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACAt                                                                                                                                     <  3:215696/149‑1 (MQ=255)
              aTAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAATGTTGCTATggttttta                                                                                                                                   <  3:131820/149‑7 (MQ=255)
              aTAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATAc                                                                                                                                   <  5:258682/149‑1 (MQ=255)
                           aGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTAt                                                                                                                                           <  5:326099/128‑1 (MQ=255)
                           aGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTAt                                                                                                                                           >  6:326099/1‑128 (MQ=255)
                           aGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATc                                                                                                                        >  2:119791/1‑147 (MQ=255)
                           aGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCa                                                                                                                      <  4:138409/149‑1 (MQ=255)
                            gTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAg                                                                                                                     <  7:218137/149‑1 (MQ=255)
                             tAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAgg                                                                                                                    >  1:345910/1‑149 (MQ=255)
                                    tCCAGTGACTGCGCCAGACACTGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCAt                                                                                                             <  5:404760/149‑1 (MQ=255)
                                    tCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCa                                                                                                              <  1:23544/148‑1 (MQ=255)
                                        gTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAATGTTGCTATggtt                                                                                                                                       <  2:22581/119‑3 (MQ=255)
                                        gTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAATGTTGCTATggtt                                                                                                                                       >  1:22581/1‑117 (MQ=255)
                                        gTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATg                                                                                                                                          <  7:223270/116‑1 (MQ=255)
                                        gTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATg                                                                                                                                          >  8:223270/1‑116 (MQ=255)
                                           aCTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGAGATACAGATAAATATCCAGGTTTTCATGCAAtt                                                                                                       <  1:120736/148‑1 (MQ=255)
                                           aCTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAAttt                                                                                                      >  1:120474/1‑149 (MQ=255)
                                                gcCAGACCCGGGCCTTCGTTATACGCAGGTATAATGGAGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGAAAAATATCCAGGTTTTAATGCAATTTGCTc                                                                                                  >  5:357199/1‑148 (MQ=255)
                                                gcCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACAtt                                                                                                                                                <  6:97094/102‑1 (MQ=255)
                                                gcCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACAtt                                                                                                                                                >  5:97094/1‑102 (MQ=255)
                                                gcCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTc                                                                                                  >  7:125582/1‑148 (MQ=255)
                                                  gaGAAACGGGGCTTCGTTTTACGCATGTATAATTGCTTCATTACAGCCTTTCTTTTGACTGTGTTTAAGTCTTAGCGTACTTAAAACCCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCaaa                                                                                               <  6:357199/148‑1 (MQ=255)
                                                  cAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACAt                                                                                                                                     <  3:217285/111‑1 (MQ=255)
                                                  cAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACAt                                                                                                                                     >  4:217285/1‑111 (MQ=255)
                                                  cAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCaaa                                                                                               <  4:250571/149‑1 (MQ=255)
                                                  cAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGGTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCaaa                                                                                               <  6:223021/149‑1 (MQ=255)
                                                        cGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCa                                                                                         <  3:20637/149‑1 (MQ=255)
                                                         gggCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAat                                                                                                                           >  1:242015/1‑114 (MQ=255)
                                                         gggCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAat                                                                                                                           <  2:242015/114‑1 (MQ=255)
                                                            ccTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATcc                                                                                                                       >  8:430200/1‑115 (MQ=255)
                                                            ccTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATcc                                                                                                                       <  7:430200/115‑1 (MQ=255)
                                                            ccTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCAtttt                                                                                     <  8:125582/149‑1 (MQ=255)
                                                                  ttATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAAt                                                                               >  8:83308/1‑149 (MQ=255)
                                                                   tataCGCAGGTATATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGa                                                                                 >  2:370435/1‑145 (MQ=255)
                                                                   tataCGCAGGTATATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGa                                                                                 <  1:370435/145‑1 (MQ=255)
                                                                     taCGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTAc                                                                            <  2:345910/149‑1 (MQ=255)
                                                                        gCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGATATGGACATACAGATAAATATACAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACaaa                                                                         >  6:114292/1‑149 (MQ=255)
                                                                          aGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTa                                                                                    >  7:207175/1‑136 (MQ=255)
                                                                          aGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTa                                                                                    <  8:207175/136‑1 (MQ=255)
                                                                           ggTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGcc                                                                      >  1:243270/1‑149 (MQ=255)
                                                                            gTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATTCAGATAAAGATCCAGGTTTGCCTGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGACt                                                                     >  3:380281/1‑149 (MQ=255)
                                                                            gTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCt                                                                     >  6:203762/1‑149 (MQ=255)
                                                                             tataATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTa                                                                                    <  8:263082/133‑1 (MQ=255)
                                                                             tataATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTa                                                                                    >  7:263082/1‑133 (MQ=255)
                                                                               taATGGCGTATATACAGCCTTTCTTTTGACTGGGTTTACGTCGTAGCGTACTTAAAAACAATAGCAACAATGCGATGGACATACAGATAAATATCCAGGTTTTAATTCAATTAGCTCAAAGAAGCATTTAATGAATTACAAAGACTTCa                                                                  >  3:369083/1‑149 (MQ=255)
                                                                                             cAGGCTTTCTTTTGACTGGGTTTACGTCTTAGAGTACTTAAAAACCATAGCAACATTGATATGGACATACAGATAAATATACAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTAtt                                                    <  5:114292/149‑1 (MQ=255)
                                                                                                ccTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGAc                                                 >  5:25018/1‑149 (MQ=255)
                                                                                                  tttctttTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCt                                               >  1:63588/1‑149 (MQ=255)
                                                                                                  tttctttTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCt                                               >  8:457604/1‑149 (MQ=255)
                                                                                                   ttctttTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCtg                                              >  5:212975/1‑149 (MQ=255)
                                                                                                       tttGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCtgt                                             <  3:188675/146‑1 (MQ=255)
                                                                                                       tttGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCtgt                                             >  4:188675/1‑146 (MQ=255)
                                                                                                        ttGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCtgtt                                            <  6:385821/146‑1 (MQ=255)
                                                                                                        ttGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCtgtt                                            <  5:203762/146‑1 (MQ=255)
                                                                                                        ttGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTc                                         >  5:156240/1‑149 (MQ=255)
                                                                                                           aCTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTAtt                                                    <  5:257402/135‑1 (MQ=255)
                                                                                                           aCTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTAtt                                                    >  6:257402/1‑135 (MQ=255)
                                                                                                           aCTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCcgc                                      >  3:268597/1‑149 (MQ=255)
                                                                                                           aCTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCcgc                                      >  8:157634/1‑149 (MQ=255)
                                                                                                             tGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCaaa                                                                                               >  6:149408/1‑90 (MQ=255)
                                                                                                             tGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCaaa                                                                                               <  5:149408/90‑1 (MQ=255)
                                                                                                             tGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTc                                                                   <  3:293352/118‑1 (MQ=255)
                                                                                                             tGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTc                                                                   >  4:293352/1‑118 (MQ=255)
                                                                                                                 tttACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAAtt                                <  4:268597/149‑1 (MQ=255)
                                                                                                                     cGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATtgtg                             <  1:119791/148‑1 (MQ=255)
                                                                                                                        cTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATATGCTCAAAGAATCATTTTATCAATTACAAAGCCTTCACCCAGATCGCTATTGACCTCTTGTCCGCGAAATTGTGCAAc                         >  2:382664/1‑149 (MQ=255)
                                                                                                                           aGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAAc                         <  6:156240/146‑1 (MQ=255)
                                                                                                                              gTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATAATTTTATGAATTACAAAGCCTTCACCAAGATCGCTATTTACATGTTGTCAGCGAAATTGTGCAACTGCACg                   >  5:19278/1‑149 (MQ=255)
                                                                                                                               tACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACg                   >  3:200094/1‑148 (MQ=255)
                                                                                                                                aCTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGAc                                                 <  3:152045/117‑1 (MQ=255)
                                                                                                                                aCTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGAc                                                 >  4:152045/1‑117 (MQ=255)
                                                                                                                                 cTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAg                <  2:243270/149‑1 (MQ=255)
                                                                                                                                  ttAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAgg               >  3:148624/1‑149 (MQ=255)
                                                                                                                                  ttAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAgg               >  6:203335/1‑149 (MQ=255)
                                                                                                                                  ttAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAg                >  6:1491/1‑148 (MQ=255)
                                                                                                                                  tgtatgtccATAGCAATGTTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAgg               >  8:462049/8‑149 (MQ=255)
                                                                                                                                    aaaaaCCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAg                <  5:1491/146‑1 (MQ=255)
                                                                                                                                    aaaaaCCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGc              >  4:20264/1‑148 (MQ=255)
                                                                                                                                      aaaCCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGAt           <  6:25018/149‑1 (MQ=255)
                                                                                                                                      aaaCCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGAt           >  1:221886/1‑149 (MQ=255)
                                                                                                                                        tccATAGCAATGTTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCa         >  4:334731/2‑149 (MQ=255)
                                                                                                                                        aCCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCa         <  7:83308/149‑1 (MQ=255)
                                                                                                                                            tAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTAGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATTAc     >  6:88843/1‑149 (MQ=255)
                                                                                                                                             aGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACg    >  2:94602/1‑149 (MQ=255)
                                                                                                                                                aaCATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTAAAAGAATCATTTTATGAATTACAAAGCCTTCACCAAGATCGCTATTGACCTGTTGTCAGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCa  >  7:334593/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATTACCCGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCA  >  NC_000913/383751‑384042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: