Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 517,380 A→T 5.1% intergenic (+18/+45) fetB → / ← ybbN iron export ABC transporter permease; peroxide resistance protein/DnaK co‑chaperone, thioredoxin‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913517,3800AT 5.1% 321.5 / 5.2 98intergenic (+18/+45)fetB/ybbNiron export ABC transporter permease; peroxide resistance protein/DnaK co‑chaperone, thioredoxin‑like protein
Reads supporting (aligned to +/- strand):  ref base A (45/48);  new base T (3/2);  total (48/50)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

Only 100 of 109 total aligned reads displayed.

TCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTACGCGTCTGACC  >  NC_000913/517233‑517526
                                                                                                                                                   |                                                                                                                                                  
tCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCgca                                                                                                                                                    <  7:279746/148‑1 (MQ=255)
 cAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCgcag                                                                                                                                                   >  7:160265/1‑148 (MQ=255)
  aGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCgcagca                                                                                                                                                 <  5:195598/149‑1 (MQ=255)
  aGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCgcag                                                                                                                                                   >  6:76531/1‑147 (MQ=255)
  aGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGAGATGCCGGATGCgcagca                                                                                                                                                 >  1:295369/1‑149 (MQ=255)
   gATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCAGTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAgcat                                                                                                                                                >  6:284110/1‑149 (MQ=255)
      tATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAgcatcg                                                                                                                                              >  5:151433/1‑148 (MQ=255)
           tGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGATGCTGCGCATCCg                                                                                                                                        <  4:393624/149‑1 (MQ=255)
            gACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCCATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCgg                                                                                                                                       >  8:379519/1‑149 (MQ=255)
             aCCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGc                                                                                                                                      >  5:158388/1‑149 (MQ=255)
               cTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGt                                                                                                                                    >  7:421994/1‑149 (MQ=255)
                   aTGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTtgt                                                                                                                                 <  4:302400/148‑1 (MQ=255)
                   aTGCTGCTCTCAACCGCCAGCTAGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTtgtg                                                                                                                                >  8:286442/1‑149 (MQ=255)
                    tgctgcTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTtgtg                                                                                                                                <  4:323028/148‑1 (MQ=255)
                    tgctgcTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTgg                                                                                                                               >  4:299660/1‑149 (MQ=255)
                      ctgctCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGtt                                                                                                                             >  2:444769/1‑149 (MQ=255)
                       tgctCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGtt                                                                                                                             >  8:178603/1‑148 (MQ=255)
                        gctCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCa                                                                                                                           >  4:135407/1‑149 (MQ=255)
                         ctctCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCAt                                                                                                                          <  5:76531/149‑1 (MQ=255)
                         ctctCAACCGCCAGCTTGTCGACCACTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCa                                                                                                                           <  1:121389/148‑1 (MQ=255)
                          tctcAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAgcatc                                                                                                                                               <  8:205503/127‑1 (MQ=255)
                          tctcAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAgcatc                                                                                                                                               >  7:205503/1‑127 (MQ=255)
                            tcAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCAtgtg                                                                                                                       >  2:141403/1‑149 (MQ=255)
                            tcAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCAtgtg                                                                                                                       >  8:118814/1‑149 (MQ=255)
                              aaCCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCAtgt                                                                                                                        <  3:135407/146‑1 (MQ=255)
                              aaCCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGcc                                                                                                                     >  8:28838/1‑149 (MQ=255)
                              aaCCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGcc                                                                                                                     >  1:152549/1‑149 (MQ=255)
                              aaCCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGcc                                                                                                                     <  7:279586/149‑1 (MQ=255)
                                     aGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAAc                                                                                                              <  8:454644/149‑1 (MQ=255)
                                     aGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAAc                                                                                                              <  6:151433/149‑1 (MQ=255)
                                     aGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAAc                                                                                                              <  1:226915/149‑1 (MQ=255)
                                          gTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGaa                                                                                                          <  2:295369/148‑1 (MQ=255)
                                             gACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATc                                                                                                      <  1:221506/149‑1 (MQ=255)
                                              aCCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTc                                                                                                                            >  1:336406/1‑126 (MQ=255)
                                              aCCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTc                                                                                                                            <  2:336406/126‑1 (MQ=255)
                                              aCCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGAt                                                                                                                  >  6:399087/1‑136 (MQ=255)
                                              aCCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGAt                                                                                                                  <  5:399087/136‑1 (MQ=255)
                                              aCCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATc                                                                                                      >  5:545/1‑148 (MQ=255)
                                              aCCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCa                                                                                                     <  1:141403/149‑1 (MQ=255)
                                                         cTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATg                                                                                          <  3:299660/149‑1 (MQ=255)
                                                         cTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATg                                                                                          >  5:120067/1‑149 (MQ=255)
                                                            cTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCa                                                                                        >  1:249063/1‑148 (MQ=255)
                                                               tttAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATAc                                                                                     <  1:444769/148‑1 (MQ=255)
                                                               tttAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACa                                                                                    <  5:284110/149‑1 (MQ=255)
                                                               tttAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACa                                                                                    <  7:212397/149‑1 (MQ=255)
                                                                  aaCCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGc                                                                                  <  6:158388/148‑1 (MQ=255)
                                                                  aaCCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCt                                                                                 >  7:41715/1‑149 (MQ=255)
                                                                      tATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTggcg                                                                             >  8:106863/1‑149 (MQ=255)
                                                                      tATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTggcg                                                                             >  7:308910/1‑149 (MQ=255)
                                                                        tCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTGCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTggcggc                                                                           <  7:286442/149‑1 (MQ=255)
                                                                             aGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATa                                                                       <  7:118814/148‑1 (MQ=255)
                                                                               ttttATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAgcatcgcatc                                                                                                                                          <  6:256623/79‑1 (MQ=255)
                                                                               ttttATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAgcatcgcatc                                                                                                                                          >  5:256623/1‑79 (MQ=255)
                                                                               ttttATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACtt                                                                    <  5:430718/149‑1 (MQ=255)
                                                                               ttttATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACtt                                                                    >  7:99883/1‑149 (MQ=255)
                                                                               ttttATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACtt                                                                    <  5:146606/149‑1 (MQ=255)
                                                                                   ataATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGa                                                                 <  8:240/148‑1 (MQ=255)
                                                                                    tacttCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGAc                                                                <  7:206807/145‑1 (MQ=255)
                                                                                    taATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGAc                                                                <  6:174315/148‑1 (MQ=255)
                                                                                      aTTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCATTACAACAATGCATACAGCTGGCGGCGATACTTCGACGc                                                              <  4:421537/148‑1 (MQ=255)
                                                                                          gcgcCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCa                                                                                        <  7:59055/118‑1 (MQ=255)
                                                                                          gcgcCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCa                                                                                        >  8:59055/1‑118 (MQ=255)
                                                                                             ccaccaGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGc                                                                                         >  8:241276/1‑114 (MQ=255)
                                                                                             ccaccaGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGc                                                                                         <  7:241276/114‑1 (MQ=255)
                                                                                              caccaGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCAt                                                      >  4:50415/1‑148 (MQ=255)
                                                                                                ccaGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATTCATACAGCTGGCGGCGATACTTCGACGCCAGTTCATCAc                                                   <  8:421994/149‑1 (MQ=255)
                                                                                                ccaGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCAc                                                   >  2:410676/1‑149 (MQ=255)
                                                                                                          gtgACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCAt                                                                                                                          >  8:322855/1‑68 (MQ=255)
                                                                                                          gtgACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCAt                                                                                                                          <  7:322855/68‑1 (MQ=255)
                                                                                                                 aaTTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCcagcgcagc                                   >  6:128321/1‑148 (MQ=255)
                                                                                                                 aaTTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGAATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCcagcgcagcg                                  >  7:363425/1‑149 (MQ=255)
                                                                                                                  aTTGAAGAAGAAATGATACGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATc                                                     <  6:23869/129‑1 (MQ=255)
                                                                                                                  aTTGAAGAAGAAATGATACGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATc                                                     >  5:23869/1‑129 (MQ=255)
                                                                                                                   ttGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAg                                <  5:128321/149‑1 (MQ=255)
                                                                                                                   ttGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAg                                <  7:106863/149‑1 (MQ=255)
                                                                                                                       agaagaaATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAAt                                                                                           <  5:231839/86‑1 (MQ=255)
                                                                                                                       agaagaaATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAAt                                                                                           >  6:231839/1‑86 (MQ=255)
                                                                                                                        gaagaaATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCAc                                                   <  5:38420/125‑1 (MQ=255)
                                                                                                                        gaagaaATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCAc                                                   >  6:38420/1‑125 (MQ=255)
                                                                                                                        gaagaaATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGAtct                           >  4:167096/1‑149 (MQ=255)
                                                                                                                           gaaATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCt                        <  7:178603/149‑1 (MQ=255)
                                                                                                                           gaaATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCt                        <  8:462387/149‑1 (MQ=255)
                                                                                                                            aaaTGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCTTGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTg                       <  8:308910/149‑1 (MQ=255)
                                                                                                                              atgatgCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCAGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGAATCACCCGTACCCAGCGCAGCGAGGATCTCCTgg                      >  7:198230/1‑148 (MQ=255)
                                                                                                                                  tgcgatgcCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGt                 >  6:319612/1‑149 (MQ=255)
                                                                                                                                    cgatgcCGGCTGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGttt               <  2:152549/149‑1 (MQ=255)
                                                                                                                                      atgcCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTa             <  7:379519/149‑1 (MQ=255)
                                                                                                                                         ccGGATGCGATGCTGCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTAcgc          >  3:279279/1‑149 (MQ=255)
                                                                                                                                           ggATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGTGGCGATACTTCGACGCCAGTGCATCACCCGTa                                              <  4:50157/111‑1 (MQ=255)
                                                                                                                                           ggATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGTGGCGATACTTCGACGCCAGTGCATCACCCGTa                                              >  3:50157/1‑111 (MQ=255)
                                                                                                                                           ggATGCGATGCTGCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTACGCGt        >  6:223472/1‑149 (MQ=255)
                                                                                                                                           ggATGCGATGCTGCGCATCCGGCATTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCa                                                    >  7:77201/1‑105 (MQ=255)
                                                                                                                                           ggATGCGATGCTGCGCATCCGGCATTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCa                                                    <  8:77201/105‑1 (MQ=255)
                                                                                                                                             aTGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTAc            <  8:233801/143‑1 (MQ=255)
                                                                                                                                             aTGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTAc            >  7:233801/1‑143 (MQ=255)
                                                                                                                                                 gcagcaTCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTg                       >  8:341051/1‑128 (MQ=255)
                                                                                                                                                 gcagcaTCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTg                       <  7:341051/128‑1 (MQ=255)
                                                                                                                                                 gcagcaTCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTACGCGTCTGAcc  <  7:237631/149‑1 (MQ=255)
                                                                                                                                                 gcagcaTCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTACGCGTCTGAcc  <  8:160265/149‑1 (MQ=255)
                                                                                                                                                 gcagcaTCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTACGCGTCTGAcc  >  1:452887/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTACGCGTCTGACC  >  NC_000913/517233‑517526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: