Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 517,384 T→G 5.1% intergenic (+22/+41) fetB → / ← ybbN iron export ABC transporter permease; peroxide resistance protein/DnaK co‑chaperone, thioredoxin‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913517,3840TG 5.1% 310.2 / 6.3 97intergenic (+22/+41)fetB/ybbNiron export ABC transporter permease; peroxide resistance protein/DnaK co‑chaperone, thioredoxin‑like protein
Reads supporting (aligned to +/- strand):  ref base T (43/49);  new base G (3/2);  total (46/51)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 106 total aligned reads displayed.

GATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTACGCGTCTGACCGTCTGC  >  NC_000913/517236‑517532
                                                                                                                                                    |                                                                                                                                                    
gATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCAGTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAgcat                                                                                                                                                      >  6:284110/1‑149 (MQ=255)
   tATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAgcatcg                                                                                                                                                    >  5:151433/1‑148 (MQ=255)
        tGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGATGCTGCGCATCCg                                                                                                                                              <  4:393624/149‑1 (MQ=255)
         gACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCCATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCgg                                                                                                                                             >  8:379519/1‑149 (MQ=255)
          aCCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGc                                                                                                                                            >  5:158388/1‑149 (MQ=255)
            cTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGt                                                                                                                                          >  7:421994/1‑149 (MQ=255)
                aTGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTtgt                                                                                                                                       <  4:302400/148‑1 (MQ=255)
                aTGCTGCTCTCAACCGCCAGCTAGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTtgtg                                                                                                                                      >  8:286442/1‑149 (MQ=255)
                 tgctgcTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTtgtg                                                                                                                                      <  4:323028/148‑1 (MQ=255)
                 tgctgcTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTgg                                                                                                                                     >  4:299660/1‑149 (MQ=255)
                   ctgctCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGtt                                                                                                                                   >  2:444769/1‑149 (MQ=255)
                    tgctCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGtt                                                                                                                                   >  8:178603/1‑148 (MQ=255)
                     gctCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCa                                                                                                                                 >  4:135407/1‑149 (MQ=255)
                      ctctCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCAt                                                                                                                                <  5:76531/149‑1 (MQ=255)
                      ctctCAACCGCCAGCTTGTCGACCACTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCa                                                                                                                                 <  1:121389/148‑1 (MQ=255)
                       tctcAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAgcatc                                                                                                                                                     >  7:205503/1‑127 (MQ=255)
                        ctcAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCAtgt                                                                                                                              >  6:146606/1‑149 (MQ=255)
                        ctcAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCAtgt                                                                                                                              >  7:462387/1‑149 (MQ=255)
                         tcAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCAtgtg                                                                                                                             >  8:118814/1‑149 (MQ=255)
                         tcAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCAtgtg                                                                                                                             >  2:141403/1‑149 (MQ=255)
                           aaCCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCAtgt                                                                                                                              <  3:135407/146‑1 (MQ=255)
                           aaCCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGcc                                                                                                                           >  8:28838/1‑149 (MQ=255)
                           aaCCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGcc                                                                                                                           >  1:152549/1‑149 (MQ=255)
                                  aGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAAc                                                                                                                    <  1:226915/149‑1 (MQ=255)
                                  aGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAAc                                                                                                                    <  6:151433/149‑1 (MQ=255)
                                  aGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAAc                                                                                                                    <  8:454644/149‑1 (MQ=255)
                                       gTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGaa                                                                                                                <  2:295369/148‑1 (MQ=255)
                                          gACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATc                                                                                                            <  1:221506/149‑1 (MQ=255)
                                           aCCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTc                                                                                                                                  >  1:336406/1‑126 (MQ=255)
                                           aCCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTc                                                                                                                                  <  2:336406/126‑1 (MQ=255)
                                           aCCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGAt                                                                                                                        >  6:399087/1‑136 (MQ=255)
                                           aCCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGAt                                                                                                                        <  5:399087/136‑1 (MQ=255)
                                           aCCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATc                                                                                                            >  5:545/1‑148 (MQ=255)
                                           aCCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCa                                                                                                           <  1:141403/149‑1 (MQ=255)
                                               ttattGCCTGCTATTTCACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGACGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATac                                                                                                       <  4:300041/149‑1 (MQ=255)
                                                      cTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATg                                                                                                <  3:299660/149‑1 (MQ=255)
                                                      cTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATg                                                                                                >  5:120067/1‑149 (MQ=255)
                                                         cTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCa                                                                                              >  1:249063/1‑148 (MQ=255)
                                                            tttAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATAc                                                                                           <  1:444769/148‑1 (MQ=255)
                                                            tttAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACa                                                                                          <  7:212397/149‑1 (MQ=255)
                                                            tttAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACa                                                                                          <  5:284110/149‑1 (MQ=255)
                                                               aaCCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGc                                                                                        <  6:158388/148‑1 (MQ=255)
                                                               aaCCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCt                                                                                       >  7:41715/1‑149 (MQ=255)
                                                                   tATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTggcg                                                                                   >  8:106863/1‑149 (MQ=255)
                                                                   tATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTggcg                                                                                   >  7:308910/1‑149 (MQ=255)
                                                                     tCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTGCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTggcggc                                                                                 <  7:286442/149‑1 (MQ=255)
                                                                          aGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATa                                                                             <  7:118814/148‑1 (MQ=255)
                                                                           gTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACt                                                                           >  7:240/1‑149 (MQ=255)
                                                                            ttttATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAgcatcgcatc                                                                                                                                                <  6:256623/79‑1 (MQ=255)
                                                                            ttttATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAgcatcgcatc                                                                                                                                                >  5:256623/1‑79 (MQ=255)
                                                                            ttttATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACtt                                                                          >  7:99883/1‑149 (MQ=255)
                                                                            ttttATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACtt                                                                          <  5:430718/149‑1 (MQ=255)
                                                                            ttttATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACtt                                                                          <  5:146606/149‑1 (MQ=255)
                                                                                ataATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGa                                                                       <  8:240/148‑1 (MQ=255)
                                                                                 tacttCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGAc                                                                      <  7:206807/145‑1 (MQ=255)
                                                                                 taATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGAc                                                                      <  6:174315/148‑1 (MQ=255)
                                                                                   aTTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCATTACAACAATGCATACAGCTGGCGGCGATACTTCGACGc                                                                    <  4:421537/148‑1 (MQ=255)
                                                                                       gcgcCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCa                                                                                              <  7:59055/118‑1 (MQ=255)
                                                                                       gcgcCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCa                                                                                              >  8:59055/1‑118 (MQ=255)
                                                                                          ccaccaGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGc                                                                                               >  8:241276/1‑114 (MQ=255)
                                                                                          ccaccaGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGc                                                                                               <  7:241276/114‑1 (MQ=255)
                                                                                           caccaGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCAt                                                            >  4:50415/1‑148 (MQ=255)
                                                                                             ccaGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATTCATACAGCTGGCGGCGATACTTCGACGCCAGTTCATCAc                                                         <  8:421994/149‑1 (MQ=255)
                                                                                             ccaGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCAc                                                         >  2:410676/1‑149 (MQ=255)
                                                                                                gTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCg                                                      >  8:259398/1‑149 (MQ=255)
                                                                                                       gtgACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCAt                                                                                                                                <  7:322855/68‑1 (MQ=255)
                                                                                                       gtgACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCAt                                                                                                                                >  8:322855/1‑68 (MQ=255)
                                                                                                              aaTTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCcagcgcagc                                         >  6:128321/1‑148 (MQ=255)
                                                                                                              aaTTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGAATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCcagcgcagcg                                        >  7:363425/1‑149 (MQ=255)
                                                                                                               aTTGAAGAAGAAATGATACGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATc                                                           >  5:23869/1‑129 (MQ=255)
                                                                                                                ttGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAg                                      <  5:128321/149‑1 (MQ=255)
                                                                                                                ttGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAg                                      <  7:106863/149‑1 (MQ=255)
                                                                                                                    agaagaaATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAAt                                                                                                 <  5:231839/86‑1 (MQ=255)
                                                                                                                    agaagaaATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAAt                                                                                                 >  6:231839/1‑86 (MQ=255)
                                                                                                                     gaagaaATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCAc                                                         <  5:38420/125‑1 (MQ=255)
                                                                                                                     gaagaaATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCAc                                                         >  6:38420/1‑125 (MQ=255)
                                                                                                                     gaagaaATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGAtct                                 >  4:167096/1‑149 (MQ=255)
                                                                                                                        gaaATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCt                              <  8:462387/149‑1 (MQ=255)
                                                                                                                        gaaATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCt                              <  7:178603/149‑1 (MQ=255)
                                                                                                                         aaaTGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCTTGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTg                             <  8:308910/149‑1 (MQ=255)
                                                                                                                           atgatgCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCAGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGAATCACCCGTACCCAGCGCAGCGAGGATCTCCTgg                            >  7:198230/1‑148 (MQ=255)
                                                                                                                               tgcgatgcCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGt                       >  6:319612/1‑149 (MQ=255)
                                                                                                                                 cgatgcCGGCTGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGttt                     <  2:152549/149‑1 (MQ=255)
                                                                                                                                   atgcCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTa                   <  6:545/149‑1 (MQ=255)
                                                                                                                                   atgcCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTa                   <  7:379519/149‑1 (MQ=255)
                                                                                                                                        ggATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGTGGCGATACTTCGACGCCAGTGCATCACCCGTa                                                    <  4:50157/111‑1 (MQ=255)
                                                                                                                                        ggATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGTGGCGATACTTCGACGCCAGTGCATCACCCGTa                                                    >  3:50157/1‑111 (MQ=255)
                                                                                                                                        ggATGCGATGCTGCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTACGCGt              >  6:223472/1‑149 (MQ=255)
                                                                                                                                        ggATGCGATGCTGCGCATCCGGCATTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCa                                                          >  7:77201/1‑105 (MQ=255)
                                                                                                                                        ggATGCGATGCTGCGCATCCGGCATTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCa                                                          <  8:77201/105‑1 (MQ=255)
                                                                                                                                          aTGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTAc                  <  8:233801/143‑1 (MQ=255)
                                                                                                                                          aTGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTAc                  >  7:233801/1‑143 (MQ=255)
                                                                                                                                              gcagcaTCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTg                             >  8:341051/1‑128 (MQ=255)
                                                                                                                                              gcagcaTCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTg                             <  7:341051/128‑1 (MQ=255)
                                                                                                                                              gcagcaTCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTACGCGTCTGAcc        <  8:160265/149‑1 (MQ=255)
                                                                                                                                              gcagcaTCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTACGCGTCTGAcc        >  1:452887/1‑149 (MQ=255)
                                                                                                                                              gcagcaTCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTACGCGTCTGAcc        <  7:237631/149‑1 (MQ=255)
                                                                                                                                                   atcgcatcCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTACGCGTCTGACCGTCTg   <  1:410676/149‑1 (MQ=255)
                                                                                                                                                    tcgcatcCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTACGCGTCTGACCGTCTgc  >  8:127296/1‑149 (MQ=255)
                                                                                                                                                    tcgcatcCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTACGCGTCTGACCGTCTgc  <  8:363425/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTACGCGTCTGACCGTCTGC  >  NC_000913/517236‑517532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: