| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 911,148 | C→T | 8.5% | intergenic (‑99/+34) | poxB ← / ← hcr | pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 911,148 | 0 | C | T | 8.5% | 169.7 / 4.2 | 59 | intergenic (‑99/+34) | poxB/hcr | pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (28/25); new base T (2/3); total (31/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 | |||||||||||
CTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATT > NC_000913/911001‑911285 | cTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGcc > 1:345739/1‑149 (MQ=255) caatgactagtcaaaaaactagtattaagactatcacttctttaagtgatattggttgtctggacatgcagggggccagactaTTTAGTTCATCTCACGGAGGGCGACGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGcc < 5:114545‑M2/64‑1 (MQ=255) ctactctaaaaactagtattaagactctcacttatttcagtgatattggttgtctggagattcagggggcccgtctaTTTAGTTCCTCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGaaa < 8:168584‑M2/72‑1 (MQ=255) cGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGaaa < 2:345739/148‑1 (MQ=255) cGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGaaa < 8:349677/148‑1 (MQ=255) actagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGcc > 3:22892‑M2/67‑130 (MQ=255) aatagttttaagactatcagttatttacgtgatattggttgtctggagattcacggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGcc < 4:22892‑M2/64‑1 (MQ=255) tagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGTCCGATGAAAGGAATATCATCgg > 1:207878‑M2/65‑149 (MQ=255) tagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGTCCGATGAAAGGAATATCATCgg > 1:214347‑M2/65‑149 (MQ=255) cGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGTCCGATGAAAGTAATATCATCgg < 6:401820/149‑1 (MQ=255) gtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCgg < 4:73830‑M2/85‑1 (MQ=255) ttaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCAt > 2:163090‑M2/60‑148 (MQ=255) gactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGTGATGGGAGAGAACGGAGGCAGTAACGGATAAATAGCCCGCTGAAAGGAATATCATCGGGCATAAGGc > 6:24658‑M2/56‑149 (MQ=255) ctatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGa > 5:77467‑M2/54‑149 (MQ=255) tatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCAACTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGACCTATTAAAGGAATATCATCGGGCATAAGGCGAt > 6:133264‑M2/53‑149 (MQ=255) aaCCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGc < 6:271730/138‑1 (MQ=255) aaCCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGc > 5:271730/1‑138 (MQ=255) tcccttatttaagtgatattggcggtctggagattcaggggcccaggctaATTAGTTCCCCTGACGGACGGGGACGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGAt < 7:347466‑M2/97‑1 (MQ=255) acttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATg > 5:429747‑M2/49‑149 (MQ=255) ttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGGAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGa > 7:401453‑M2/47‑142 (MQ=255) tgtttCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCga < 1:432088/148‑1 (MQ=255) agtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCAGAAGAAAGGAATATCATCGGGCAGAAGGCGATTATGCGAGAAcc > 2:97320‑M2/40‑148 (MQ=255) gatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAAt > 1:405690‑M2/37‑149 (MQ=255) ggtctggagattcagcgggcccttctaTGTAGCTCCTCTGACGGAGGGGTAAGGGATGGGAGAGAAAGGAGGCACTAACGGGTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCGCCCTGCg < 6:139903‑M2/122‑1 (MQ=255) cTGAATGTGATTACGGTAACAAGTTTAGTTCATCTGAAGGAGTGGGAAGGTATTGTAGAGTAAGGACCCACTACAGGTTAAATAGCCCGATGAAACTTATATCATAGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGa < 4:315857/147‑1 (MQ=255) ggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATgg < 2:405690‑M2/127‑1 (MQ=255) attcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAg > 6:111869‑M2/19‑148 (MQ=255) agggggcaagtctaTTAAGTTCATCTGACGTAGGGGGAAGGCATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCTTAAGCCGATTATGCGACAGCCAAAACCCCAGGCGGATGGCAGGAg > 6:365523‑M2/15‑147 (MQ=255) gggggccagtctaTTTAGTTCTCCTGACGGAGGGGGACGGGTTGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAg < 6:429747‑M2/136‑1 (MQ=255) agtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTa > 8:306517‑M2/7‑149 (MQ=255) gtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTAc > 8:421544‑M2/6‑149 (MQ=255) tttAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGAt > 1:469038/1‑125 (MQ=255) tttAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGAt < 2:469038/125‑1 (MQ=255) tttAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATg < 4:122101/126‑1 (MQ=255) tttAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATg > 3:122101/1‑126 (MQ=255) tttAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAAc > 2:313789/1‑149 (MQ=255) ttAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAAcc > 2:223205/1‑149 (MQ=255) gTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCtt > 3:266816/1‑148 (MQ=255) aTCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCa > 4:50429/1‑132 (MQ=255) aTCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCa < 3:50429/132‑1 (MQ=255) gACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGAttt > 4:244109/1‑149 (MQ=255) aCGGAGGGGGAAGGGATGGGAGAGAAAGTAGGCACTAACGGTTAAATAGCCCGATGAAAGGAAAATCATAGGGCATAAGGCGATTATGCGATAACCAAATCCCCCTGCGGATGTCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTc > 6:451533/1‑149 (MQ=255) cGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCg < 8:216399/149‑1 (MQ=255) ggggAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTc < 7:306517/149‑1 (MQ=255) gggAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGTCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCg < 2:214347/149‑1 (MQ=255) gggAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGTCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCg < 2:207878/149‑1 (MQ=255) ggAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCa < 1:223205/148‑1 (MQ=255) gagagaAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCAtt < 4:84261/149‑1 (MQ=255) gagagaAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCAtt < 5:111869/149‑1 (MQ=255) gagagaAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCAtt > 6:256486/1‑149 (MQ=255) aaaGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGtgct > 8:438753/1‑149 (MQ=255) aaaGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGtgct > 4:353225/1‑149 (MQ=255) ggaggCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGc > 2:209500/1‑98 (MQ=255) ggaggCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGc < 1:209500/98‑1 (MQ=255) gaggCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGtgctg > 4:250041/1‑146 (MQ=255) gaggCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGtgctg < 3:250041/146‑1 (MQ=255) ggCACTAACGGTTAAATAGCCCGATGAAAGGAATATCCTCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCAc < 5:24658/149‑1 (MQ=255) aCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGt < 5:256486/149‑1 (MQ=255) aCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCGCACCg > 5:275361/1‑148 (MQ=255) aCGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGtata < 1:163090/148‑1 (MQ=255) ggTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATAtt > 3:211698/1‑148 (MQ=255) gTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGc < 4:362766/103‑1 (MQ=255) gTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGc > 3:362766/1‑103 (MQ=255) | CTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATT > NC_000913/911001‑911285 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |