Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 980,167 A→T 5.6% E1281V (GAA→GTA)  mukB → chromosome condensin MukBEF, ATPase and DNA‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913980,1670AT 5.6% 291.9 / 5.5 90E1281V (GAA→GTA) mukBchromosome condensin MukBEF, ATPase and DNA‑binding subunit
Reads supporting (aligned to +/- strand):  ref base A (36/49);  new base T (2/3);  total (38/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

CTGGCGATCAGTTCCCGCAGCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGTATCAACGTCTTAACCCGCAGATTGATATGGGGCAGCGCACGC  >  NC_000913/980022‑980313
                                                                                                                                                 |                                                                                                                                                  
cTGGCGATCAGTTCCCGCAGCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAac                                                                                                                                                 >  6:419017/1‑149 (MQ=255)
   gCGATCAGTTCCCGCAGCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAacgc                                                                                                                                               <  5:66796/148‑1 (MQ=255)
      aTCAGTTCCCGCAGCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAacgcacgc                                                                                                                                           <  8:173194/149‑1 (MQ=255)
      aTCAGTTCCCGCAGCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAacgcacgc                                                                                                                                           >  5:327747/1‑149 (MQ=255)
         aGTTCCCGCAGCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTTTcacgcacgttca                                                                                                                                        <  7:315041/149‑5 (MQ=255)
           ttaccGCAGCGTGGCGAACATCATTCGCAAAACCATTCAGCGAGAGAAGAACAGTATCCGTATGCTCAACCAGGGGTTGCATAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGa                                                                                                                                                    <  2:251166/132‑1 (MQ=255)
           ttCCCGCAGCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTTTcacgcacgttc                                                                                                                                         >  2:63236/1‑143 (MQ=255)
           ttCCCGCAGCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTTTcacgcacgttc                                                                                                                                         <  1:63236/146‑4 (MQ=255)
           ttCCCGCAGCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGGCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGa                                                                                                                                                    >  1:251166/1‑135 (MQ=255)
              ccGCAGCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTAc                                                                                                                                   <  4:393299/149‑1 (MQ=255)
              ccGCAGCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTAc                                                                                                                                   >  2:299653/1‑149 (MQ=255)
               cGCAGCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACt                                                                                                                                  <  1:134532/149‑1 (MQ=255)
                gCAGCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTg                                                                                                                                 >  3:216082/1‑149 (MQ=255)
                gCAGCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGGGAACAGCGTGCGTCTCAACGTGAACGTGCGGGAAACGCACGCCATGCTACTg                                                                                                                                 <  8:182938/149‑1 (MQ=255)
                 cAGCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTgg                                                                                                                                <  2:394083/149‑1 (MQ=255)
                  aGCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGa                                                                                                                               <  6:414989/149‑1 (MQ=255)
                  aGCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGa                                                                                                                               <  4:41242/149‑1 (MQ=255)
                   gCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGAt                                                                                                                              <  3:168696/149‑1 (MQ=255)
                    cGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCTACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGAtg                                                                                                                             <  6:61695/149‑1 (MQ=255)
                     gTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGAtgt                                                                                                                            >  8:332136/1‑149 (MQ=255)
                     gTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGAtgt                                                                                                                            <  7:173909/149‑1 (MQ=255)
                       ggCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGc                                                                                                                          >  8:447653/1‑149 (MQ=255)
                          gAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGctct                                                                                                                       <  8:419253/149‑1 (MQ=255)
                            aCATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGctctct                                                                                                                     <  1:21197/149‑1 (MQ=255)
                            aCATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGctctct                                                                                                                     >  8:423733/1‑149 (MQ=255)
                               tcatTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCTACCAGGGGTTGCAGAACGTTTCGTTTGGTGAGGTGATCAGCGTGAGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGaa                                                                                                                  <  5:53947/149‑1 (MQ=255)
                                 atTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGaa                                                                                                                  >  3:330468/1‑147 (MQ=255)
                                 atTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGaa                                                                                                                  >  2:136109/1‑147 (MQ=255)
                                 atTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACa                                                                                                                <  7:298241/149‑1 (MQ=255)
                                  ttCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCAt                                                                                                                                        >  4:11763/1‑124 (MQ=255)
                                  ttCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCAt                                                                                                                                        <  3:11763/124‑1 (MQ=255)
                                  ttCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAg                                                                                                               >  4:210445/1‑149 (MQ=255)
                                     gCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCAc                                                                                                            >  5:22677/1‑149 (MQ=255)
                                     gCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCAc                                                                                                            <  5:451757/149‑1 (MQ=255)
                                       aaaaCCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCAc                                                                                                            >  2:440161/1‑147 (MQ=255)
                                            cATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGagcagc                                                                                                     <  4:330468/149‑1 (MQ=255)
                                            cATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGagcag                                                                                                      >  4:174506/1‑148 (MQ=255)
                                                 aGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGa                                                                                                                   >  4:126948/1‑130 (MQ=255)
                                                 aGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGa                                                                                                                   <  3:126948/130‑1 (MQ=255)
                                                    gcgAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGAt                                                                                             <  4:216082/149‑1 (MQ=255)
                                                    gcgAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGAt                                                                                             >  3:156940/1‑149 (MQ=255)
                                                         cAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGtt                                                                                        >  1:203113/1‑149 (MQ=255)
                                                           gAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGttt                                                                                       >  6:636/1‑148 (MQ=255)
                                                                gtatccgtatGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAg                                                                                  >  6:287787/1‑148 (MQ=255)
                                                                   tccgtatGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGttt                                                                                       <  1:311286/140‑1 (MQ=255)
                                                                   tccgtatGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGttt                                                                                       >  2:311286/1‑140 (MQ=255)
                                                                     cgtatGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCg                                                                            <  3:210445/149‑1 (MQ=255)
                                                                     cgtatGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCg                                                                            >  4:184402/1‑149 (MQ=255)
                                                                          gCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTaa                                                                                     >  6:258066/1‑135 (MQ=255)
                                                                          gCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTATCAGCAACCGTTTGa                                                                       >  7:451885/1‑149 (MQ=255)
                                                                          gCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTCAACGTGCGTGAAAAGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTaa                                                                                     <  5:258066/135‑1 (MQ=255)
                                                                           cTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGAc                                                                      <  5:636/149‑1 (MQ=255)
                                                                            tCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCAc                                                                                                            >  2:425849/1‑110 (MQ=255)
                                                                            tCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCAc                                                                                                            <  1:425849/110‑1 (MQ=255)
                                                                              aaCCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCtt                                                                   >  7:163564/1‑149 (MQ=255)
                                                                                ccAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTtct                                                                 >  5:289570/1‑149 (MQ=255)
                                                                                ccAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTtct                                                                 >  2:114768/1‑149 (MQ=255)
                                                                                 cAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTtctc                                                                <  2:203113/149‑1 (MQ=255)
                                                                                 cAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTtctc                                                                <  5:419017/149‑1 (MQ=255)
                                                                                 cAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTtctc                                                                <  7:423733/149‑1 (MQ=255)
                                                                                   ggggTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCg                                                               >  6:296431/1‑148 (MQ=255)
                                                                                     ggTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGaa                                                            <  8:451885/149‑1 (MQ=255)
                                                                                         gCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAATGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAgcgc                                                        >  4:101202/1‑149 (MQ=255)
                                                                                          cAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAATAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCt                                                       >  7:271809/1‑149 (MQ=255)
                                                                                           aGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAgcgcag                                                      >  3:165920/1‑147 (MQ=255)
                                                                                            gAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTgg                                                     <  1:440161/149‑1 (MQ=255)
                                                                                            gAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTgg                                                     <  8:163564/149‑1 (MQ=255)
                                                                                             aaCGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGc                                                    >  2:71855/1‑149 (MQ=255)
                                                                                                 tATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGaac                                                <  7:332136/149‑2 (MQ=255)
                                                                                                 tATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGaaa                                                >  8:77970/1‑149 (MQ=255)
                                                                                                     gTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGt                                            <  7:43141/149‑1 (MQ=255)
                                                                                                     gTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGt                                            <  6:289570/149‑1 (MQ=255)
                                                                                                     gTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGAACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTg                                             <  5:287787/148‑1 (MQ=255)
                                                                                                           tCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGTATCAAc                                      <  1:71855/149‑1 (MQ=255)
                                                                                                           tCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGTATCAAc                                      <  6:327747/149‑1 (MQ=255)
                                                                                                             aGGTGAACAGCGTGCGTCTCAACTTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGTATCAACGt                                    <  1:299653/149‑1 (MQ=255)
                                                                                                             aGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGTATCAACGt                                    >  4:181663/1‑149 (MQ=255)
                                                                                                              ggTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGTATCAACGTc                                   <  4:156940/149‑1 (MQ=255)
                                                                                                               gTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGTATCAACGTCt                                  <  6:76200/149‑1 (MQ=255)
                                                                                                                  aaCAGCTTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAATAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGTATCAACGTCTTa                                <  8:271809/148‑1 (MQ=255)
                                                                                                                    cAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGTATCAACGTCTTAAcc                             <  3:174506/149‑1 (MQ=255)
                                                                                                                      gcgtgcgtCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGTATCAACGTCTTAACCCg                           <  7:447653/149‑1 (MQ=255)
                                                                                                                            gtCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGTATCAACGTCTTAACCCGCAGAtt                     <  1:136109/149‑1 (MQ=255)
                                                                                                                             tctcAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGTATCAACGTCTTAACCCGCAGATTg                    <  7:415736/149‑1 (MQ=255)
                                                                                                                                 aacgtgaacgtgCGTGAAACGCACGCCATGCTACTGGATGTGCTATCTGAACAGCACCAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCATCTCGGAAGCGCTGGCGAAACTGTATCAACTTCTTAAACCGCAGATTGatat                >  5:339462/1‑149 (MQ=255)
                                                                                                                                  acgtgaatgtgCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGTATCAACGTCTTAACCCGCAGATTGATATg               <  3:101202/149‑1 (MQ=255)
                                                                                                                                       ggcgtgcgttTCACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGTATCAACGTCTTAAc                              >  6:135131/3‑129 (MQ=255)
                                                                                                                                       ggcgtgcgttTCACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGTATCAACGTCTTAAc                              <  5:135131/127‑1 (MQ=255)
                                                                                                                                       aacgtgCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCCGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGTATCAACGTCTTAACCCGCAGATTGATATgggg            >  4:65731/1‑147 (MQ=255)
                                                                                                                                          gtgcgtgAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGTATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGTATCAACGTCTTAACCCGCAGATTGATATGGGGCAgcg       <  1:114768/149‑1 (MQ=255)
                                                                                                                                             cgtgAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGAGCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGTATCAACGTCTTAACCCGCAGATTGATATGGGGCAGCGCAc    <  3:184402/149‑1 (MQ=255)
                                                                                                                                               tgAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGTATCAACGTCTTAACCCGCAGATTGATATGGGGCAGCGCAcgc  <  5:296431/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CTGGCGATCAGTTCCCGCAGCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATCAGGATCTGTTTAACAGCAACCGTTTGACCTTCTCGGAAGCGCTGGCGAAACTGTATCAACGTCTTAACCCGCAGATTGATATGGGGCAGCGCACGC  >  NC_000913/980022‑980313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑