Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,271,320 A→T 6.3% I177N (ATT→AAT)  chaA ← calcium/sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,271,3200AT 6.3% 335.3 / 8.0 105I177N (ATT→AAT) chaAcalcium/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (47/51);  new base T (3/4);  total (50/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

GATAGCATGCCACAGGCTGCTATGGGCAGACGGTTTACCGTGATGCGGGTCGTCATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTAC  >  NC_000913/1271175‑1271467
                                                                                                                                                 |                                                                                                                                                   
gATAGCATGCCACAGGCTGCTATGGGCAGACGGTTTACCGTGATGCGGGTCGTCATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAAtt                                                                                                                                                   >  5:249753/1‑148 (MQ=255)
  tAGCATGCCACAGGCTGCTATGGGCAGACGGTTTACCGTGATGCGGGTCGTCATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAAt                                                                                                                                                <  2:450712/149‑1 (MQ=255)
         ccACAGGCTGCTATGGGCAGACGGTTTACCGTGATGCGGGTCGTCATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCa                                                                                                                                         <  8:233139/149‑1 (MQ=255)
          cacaGGCTGCTATGGGCAGACGGTTTACCGTGATGCGGGTCGTCATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAg                                                                                                                                        >  6:441126/1‑149 (MQ=255)
           acaGGCTGCTATGGGCAGACGGTTTACCGTGATGCGGGTCGTCATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAg                                                                                                                                        >  6:387894/1‑148 (MQ=255)
           acaGGCTGCTATGGGCAGACGGTTTACCGTGATGCGGGTCGTCATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGt                                                                                                                                       <  1:203057/149‑1 (MQ=255)
             aGGCTGCTATGGGCAGACGGTTTACCGTGATGCGGGTCGTCATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTaa                                                                                                                                     >  5:259984/1‑149 (MQ=255)
               gctgctATGGGCAGACGGTTTACCGTGATGCGGGTCGTCATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACg                                                                                                                                   >  1:225165/1‑149 (MQ=255)
                   ctATGGGCAGACGGTTTACCGTGATGCGGGTCGTCATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGcc                                                                                                                                 <  5:441126/147‑1 (MQ=255)
                         gCAGACGGTTTACCGTGATGCGGGTCGTCATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGc                                                                                                                                  >  7:430944/1‑140 (MQ=255)
                         gCAGACGGTTTACCGTGATGCGGGTCGTCATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGc                                                                                                                                  <  8:430944/140‑1 (MQ=255)
                          cAGACGGTTTACCGTGATGCGGGTCGTCATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGtt                                                                                                                        >  2:337669/1‑149 (MQ=255)
                             aCGGTTTACCGTGATGCGGGTCGTCATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGaa                                                                                                                     <  6:259984/149‑1 (MQ=255)
                              cGGTTTACCGTGATGCGGGTCGTCATCATCACTGTCATCTTCGTGCTCGTATACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGaa                                                                                                                     <  3:197898/148‑1 (MQ=255)
                                gTTTACCGTGATGCTGGTCGTCATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGaaaaa                                                                                                                  <  5:64128/149‑1 (MQ=255)
                                 tttACCGTGATGCGGGTCGTCATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATATATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAAt                                                                                                                 >  1:433086/1‑149 (MQ=255)
                                   tACCGTGATGCGGGTCGTCATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAAtt                                                                                                                <  7:53712/148‑1 (MQ=255)
                                           tGCGGGTCGTCATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCgcagg                                                                                                       >  3:116344/1‑149 (MQ=255)
                                               ggTCGTCATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGAGCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCaga                                                                                                   <  5:387894/149‑1 (MQ=255)
                                                gtcgtcATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCagag                                                                                                  >  1:460051/1‑149 (MQ=255)
                                                   gtcATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCa                                                                                               >  8:319605/1‑149 (MQ=255)
                                                    tcatcatcaCTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATATATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCAt                                                                                              <  2:433086/149‑1 (MQ=255)
                                                    tcatcatcaCTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGtt                                                                                                                        <  8:27920/123‑1 (MQ=255)
                                                    tcatcatcaCTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCAt                                                                                              >  1:229862/1‑149 (MQ=255)
                                                     catcatcaCTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCAtt                                                                                             >  1:310197/1‑149 (MQ=255)
                                                        catcaCTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCTGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGa                                                                                          >  8:344581/1‑149 (MQ=255)
                                                         atcaCTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGaa                                                                                         <  1:463655/149‑1 (MQ=255)
                                                         atcaCTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGGCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGaa                                                                                         <  6:28080/149‑1 (MQ=255)
                                                            aCTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATTCATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCTGTTGAAAAATTGGCCGCAGTCAGAGCCATTGGAAAt                                                                                       <  2:389332/148‑1 (MQ=255)
                                                             cTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATAc                                                                                     <  1:195288/149‑1 (MQ=255)
                                                              tGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATAc                                                                                     >  6:32506/1‑148 (MQ=255)
                                                              tGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATAc                                                                                     >  4:88512/1‑148 (MQ=255)
                                                                tCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCa                                                                                   <  4:412533/148‑1 (MQ=255)
                                                                tCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCa                                                                                   <  7:7417/148‑1 (MQ=255)
                                                                tCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCa                                                                                   <  3:88512/148‑1 (MQ=255)
                                                                tCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAg                                                                                  <  7:175212/149‑1 (MQ=255)
                                                                  aTCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTa                                                                                >  8:343291/1‑149 (MQ=255)
                                                                   tCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATAAGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACTCCACACAGGTTGAAAAATTCGCCGCAGGCAGAGCCAATGGAAATACCAGAAc                                                                               >  1:236235/1‑149 (MQ=255)
                                                                     ttCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTAc                                                                                                                                           >  6:349303/1‑87 (MQ=255)
                                                                     ttCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTAc                                                                                                                                           <  5:342951/87‑1 (MQ=255)
                                                                     ttCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTAc                                                                                                                                           >  6:342951/1‑87 (MQ=255)
                                                                     ttCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTAc                                                                                                                                           <  5:349303/87‑1 (MQ=255)
                                                                              gTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCa                                                                    <  2:225165/149‑1 (MQ=255)
                                                                                aGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCATGACCGGGTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAgg                                                                  >  6:213339/1‑149 (MQ=255)
                                                                                 gACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAggg                                                                 >  3:240478/1‑149 (MQ=255)
                                                                                  acaaacaaACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAAtt                                                                                                                                                   >  7:231595/1‑66 (MQ=255)
                                                                                  acaaacaaACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAAtt                                                                                                                                                   <  8:231595/66‑1 (MQ=255)
                                                                                   caaacaaaATTTGATGCGTTTTGGTCTGGATCAGCAAATATACGCCATACATTGCCGCAGAAATTAATGCTACCTGTAACGCCTGTCCGTTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAgggg                                                                <  2:236235/148‑1 (MQ=255)
                                                                                    aaacaaacTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAggggg                                                               >  8:115792/1‑148 (MQ=255)
                                                                                    aaacaaacTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAggggg                                                               >  5:381183/1‑148 (MQ=255)
                                                                                     aacaaacTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGaa                                                             >  2:378819/1‑149 (MQ=255)
                                                                                      acaaacTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAggg                                                                 >  8:19148/1‑144 (MQ=255)
                                                                                      acaaacTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAggg                                                                 <  7:19148/144‑1 (MQ=255)
                                                                                      acaaacTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGaa                                                             >  6:168650/1‑148 (MQ=255)
                                                                                      acaaacTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGaa                                                             >  5:447448/1‑148 (MQ=255)
                                                                                       caaacTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCAGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTTAAAAATTCGCAGCAGTCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGaa                                                             >  3:151987/1‑147 (MQ=255)
                                                                                        aaacTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAg                                                          >  5:425170/1‑149 (MQ=255)
                                                                                         aacTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAgc                                                         >  2:413673/1‑149 (MQ=255)
                                                                                          acTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAgcg                                                        <  6:105652/149‑1 (MQ=255)
                                                                                            tttGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAggggg                                                               >  6:385378/1‑140 (MQ=255)
                                                                                            tttGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAggggg                                                               <  5:385378/140‑1 (MQ=255)
                                                                                              tGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCaa                                                     <  4:116344/148‑1 (MQ=255)
                                                                                                aTGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAAtt                                                   <  7:343291/148‑1 (MQ=255)
                                                                                                 tGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTa                                                  >  5:284231/1‑148 (MQ=255)
                                                                                                  gctgtttGGTCGGGATCAGCAAACATACGCCATACATTGCCGACGAATTTAATGCTACCAGTAACGCCTGACCGGTTGATCAATTCGCCGCAGGCAGAGCCATTGGAAATACCTGGACGATTATCGCCAGGGGGAACAGCGCAATTaaa                                                <  8:459634/145‑1 (MQ=255)
                                                                                                  gCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTaaa                                                >  2:195910/1‑149 (MQ=255)
                                                                                                   cGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTaaa                                                <  2:310197/148‑1 (MQ=255)
                                                                                                    gTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATa                                              >  6:168280/1‑149 (MQ=255)
                                                                                                    gTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATa                                              >  5:322797/1‑149 (MQ=255)
                                                                                                    gTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATa                                              <  6:322797/149‑1 (MQ=255)
                                                                                                        tGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTg                                           <  1:199554/148‑1 (MQ=255)
                                                                                                         ggTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGc                                          <  1:195910/148‑1 (MQ=255)
                                                                                                          gTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCtt                                        <  2:460051/149‑1 (MQ=255)
                                                                                                           tCTGGATCAGCAAAAATACGCAATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTCCGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCtt                                        >  4:359283/1‑148 (MQ=255)
                                                                                                            cTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGa                                      >  7:65208/1‑149 (MQ=255)
                                                                                                             tGGATCAGCAAAAATACGCCATACATTGCCGCAGAATTTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCtt                                        <  4:421607/146‑1 (MQ=255)
                                                                                                             tGGATCAGCAAAAATACGCCATACATTGCCGCAGAATTTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCtt                                        <  4:414907/146‑1 (MQ=255)
                                                                                                             tGGATCAGCAAAAATACGCCATACATTGCCGCAGAATTTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCtt                                        <  4:421612/146‑1 (MQ=255)
                                                                                                             tGGATCAGCAAAAATACGCCATACATTGCCGCAGAATTTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCtt                                        >  3:421607/1‑146 (MQ=255)
                                                                                                             tGGATCAGCAAAAATACGCCATACATTGCCGCAGAATTTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCtt                                        >  3:421612/1‑146 (MQ=255)
                                                                                                             tGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGa                                      >  6:176496/1‑148 (MQ=255)
                                                                                                             tGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGa                                      >  1:129247/1‑148 (MQ=255)
                                                                                                             tGGATCAGCAAAAAAACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCAATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGa                                      >  1:364856/1‑148 (MQ=255)
                                                                                                               gATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGa                                      <  7:231487/146‑1 (MQ=255)
                                                                                                               gATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGa                                      <  5:176496/146‑1 (MQ=255)
                                                                                                               gATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATa                                    <  5:168650/148‑1 (MQ=255)
                                                                                                                agcAGCAAAAATACGCCATACTTTGCCGCAGAAATTAATGCTACCAGTAAGGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATAGCAGTACGATTATCGCCAGGGGGATCAGCTCAATTAATTACTGCTTGATagc                                  <  4:151987/147‑3 (MQ=255)
                                                                                                                         aaTACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGAtt                         <  6:381183/149‑1 (MQ=255)
                                                                                                                          aTACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTc                        <  6:284231/149‑1 (MQ=255)
                                                                                                                          aTACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTc                        <  2:129247/149‑1 (MQ=255)
                                                                                                                                cATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATAc                                                                                     >  8:130329/1‑82 (MQ=255)
                                                                                                                                cATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATAc                                                                                     <  7:130329/82‑1 (MQ=255)
                                                                                                                                 aTACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGATCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATAtt                 <  5:32506/149‑1 (MQ=255)
                                                                                                                                     aTTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGt             <  7:115792/149‑1 (MQ=255)
                                                                                                                                     aTTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGt             >  4:224161/1‑149 (MQ=255)
                                                                                                                                            cAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGACCAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAAc      <  1:337669/149‑1 (MQ=255)
                                                                                                                                            cAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAAc      <  1:413673/149‑1 (MQ=255)
                                                                                                                                              gAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATc                                                                        >  2:305788/1‑81 (MQ=255)
                                                                                                                                              gAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATc                                                                        <  1:305788/81‑1 (MQ=255)
                                                                                                                                                aaTTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTAc  <  2:229862/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GATAGCATGCCACAGGCTGCTATGGGCAGACGGTTTACCGTGATGCGGGTCGTCATCATCACTGTCATCTTCGTGCTCGTAGACAAACAAACTTTGATGCGTTTTGGTCTGGATCAGCAAAAATACGCCATACATTGCCGCAGAAATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTAC  >  NC_000913/1271175‑1271467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: