Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,698,587 T→A 6.2% A198A (GCA→GCT) ‡ malI ← transcriptional repressor of Mal regulon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,698,5870TA 6.2% 200.6 / 3.2 64A198A (GCA→GCT) ‡malItranscriptional repressor of Mal regulon
Reads supporting (aligned to +/- strand):  ref base T (30/30);  new base A (2/2);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

CCACGGCACTGATGGTCGGGTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGG  >  NC_000913/1698439‑1698728
                                                                                                                                                    |                                                                                                                                             
ccACGGCACTGATGGTCGGGTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCt                                                                                                                                               <  8:458501/149‑1 (MQ=255)
              gTCGGGTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGa                                                                                                                                 <  7:259024/149‑1 (MQ=255)
              gTCGGGTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTGCAGAACGGGttgggg                                                                                                                                  <  4:247375/148‑5 (MQ=255)
               tCGGGTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCAAATCGCTATGAAACGGCAGGACAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCAGTTCTGCACGGGTTAATGa                                                                                                                                 <  2:217667/148‑1 (MQ=255)
                cGGGTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAgg                                                                                                                               <  8:112431/149‑1 (MQ=255)
                 gggTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAgg                                                                                                                               >  5:76712/1‑148 (MQ=255)
                    ttATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACt                                                                                                                           <  2:462345/149‑1 (MQ=255)
                       tGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTg                                                                                                                        >  3:347690/1‑149 (MQ=255)
                         aCGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAACCGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGcc                                                                                                                      <  5:193530/149‑1 (MQ=255)
                         aCGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGc                                                                                                                       <  1:38705/148‑1 (MQ=255)
                             aaaaGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCCAATTTTAGTAGAGTGGCACAATAGCCCCCAACCCGTTCTGCACGGGTTATTGAGGAACTTTGCCCGcc                                                                                                                  >  3:186354/1‑149 (MQ=255)
                                    ccGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCccagccag                                                                                                           <  4:347690/149‑1 (MQ=255)
                                     cGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAgg                                                                                                          <  1:18893/149‑1 (MQ=255)
                                     cGTGATAGCTTCCGCGGCCTGCTTCTGGCTGGAAGTGCACTCCAACACCCCATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAgg                                                                                                          >  2:413634/1‑149 (MQ=255)
                                            gCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATcc                                                                                                    <  2:421421/148‑1 (MQ=255)
                                            gCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCg                                                                                                   >  5:364376/1‑149 (MQ=255)
                                                ccGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGTGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt                                                                                                 <  1:27972/147‑1 (MQ=255)
                                                     gcttgcttCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGcc                                                                                           >  3:152446/1‑148 (MQ=255)
                                                        tgcttCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCAtt                                                                                       >  4:165823/1‑149 (MQ=255)
                                                                gctggAAGTGCCCTCCCAACCCCACTCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGGTGCACAATCGCCCCCAACCCGTTCTGCCCGGGTTAATGATGAACTTTGCCCTCCCAGCCAGGCGCTCCGCTGATGCCCATTGCGAAt                                                                                 <  8:426792/147‑1 (MQ=255)
                                                                gctggAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCCACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATga                                                                               <  3:218617/149‑1 (MQ=255)
                                                                gctggAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCccagccag                                                                                                           >  8:404402/1‑121 (MQ=255)
                                                                gctggAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAAt                                                                                 <  4:152446/147‑1 (MQ=255)
                                                                   ggAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACttt                                                                                                                         <  4:40261/104‑1 (MQ=255)
                                                                   ggAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACttt                                                                                                                         >  3:40261/1‑104 (MQ=255)
                                                                          cACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGt                                                                     >  1:43958/1‑149 (MQ=255)
                                                                          cACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGt                                                                     <  3:165823/149‑1 (MQ=255)
                                                                          cACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGt                                                                     >  4:328347/1‑149 (MQ=255)
                                                                             tCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaa                                                                  >  3:199486/1‑149 (MQ=255)
                                                                               cAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGTGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac                                                                 >  1:434302/1‑148 (MQ=255)
                                                                               cAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac                                                                 >  7:74254/1‑148 (MQ=255)
                                                                               cAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAAAAGCCCCCAACCCCTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGCTGCCCATTGCGAATCAGATGCTCCGTcaac                                                                 >  1:143211/1‑148 (MQ=255)
                                                                                aaCACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTTCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCCGGCGATCCGCTGATGCCCATTGCGAATCAGATGCTCCGTcaacaa                                                               >  7:420617/1‑149 (MQ=255)
                                                                                aaCACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaacaa                                                               >  1:168171/1‑149 (MQ=255)
                                                                                    cccAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACtgt                                                           <  8:74254/149‑1 (MQ=255)
                                                                                    cccAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGAGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACtgt                                                           <  2:434302/149‑1 (MQ=255)
                                                                                      cAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTc                                                                    <  3:62762/138‑1 (MQ=255)
                                                                                      cAATCCCTATTAAACTGCAGGCCAAAATTTAGAACAGTTGCACAAAAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCCGGCGATCCGGTGATGCCCATTGCGAATGAGATGATCCTTc                                                                    >  4:62762/1‑138 (MQ=255)
                                                                                       aaTCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAAt                                                                                 <  5:333215/124‑1 (MQ=255)
                                                                                       aaTCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAAt                                                                                 >  6:333215/1‑124 (MQ=255)
                                                                                           gCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCt                                                    <  3:328347/149‑1 (MQ=255)
                                                                                             tATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTg                                                   >  1:260432/1‑148 (MQ=255)
                                                                                             tATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTg                                                   >  2:235725/1‑148 (MQ=255)
                                                                                               tGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCAt                                                <  6:76712/149‑1 (MQ=255)
                                                                                                     ggCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTc                                          >  1:458883/1‑149 (MQ=255)
                                                                                                        aGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCgg                                       <  6:364376/149‑1 (MQ=255)
                                                                                                        aGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCgg                                       <  2:168171/149‑1 (MQ=255)
                                                                                                            cAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGa                                   <  4:199486/149‑1 (MQ=255)
                                                                                                                         agTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAAcat                      >  8:331298/1‑149 (MQ=255)
                                                                                                                           ttGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATc                           <  5:275266/142‑1 (MQ=255)
                                                                                                                           ttGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATc                           >  6:275266/1‑142 (MQ=255)
                                                                                                                               acaATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCaa                <  1:413634/149‑1 (MQ=255)
                                                                                                                                 aaTAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAAcatcat                   <  7:286101/144‑1 (MQ=255)
                                                                                                                                 aaTAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAAcatcat                   >  8:286101/1‑144 (MQ=255)
                                                                                                                                 aaTAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACatcatc                  >  5:395555/1‑145 (MQ=255)
                                                                                                                                     gCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAAc          <  2:260432/149‑1 (MQ=255)
                                                                                                                                      tcattaaCCCGTGCAGAACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAAc                        >  2:2969/6‑134 (MQ=255)
                                                                                                                                      tcattaaCCCGTGCAGAACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAAc                        <  1:2969/129‑1 (MQ=255)
                                                                                                                                      cccccAACCCGTTCTGCACGGGTTAATTAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACt         >  8:444274/1‑149 (MQ=255)
                                                                                                                                        cggatcgcGTTCTGCTCGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGTTCCGTTGATGCCGTTTGCGAATGAGATGCTCGGTCAACAACTGTGCAGCCTGCTTGTTGTCCGGGCGAACCGTATCAACATCATCTAGATAACTgg       <  2:143211/142‑1 (MQ=255)
                                                                                                                                        cccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACt         <  7:288210/147‑1 (MQ=255)
                                                                                                                                        cccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACt         >  8:288210/1‑147 (MQ=255)
                                                                                                                                        cccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTgg       >  3:69557/1‑149 (MQ=255)
                                                                                                                                            aCCCGTGCAGAACGGGTTAATGAGGAACTTTGCCCTCTCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACg   >  4:421435/1‑149 (MQ=255)
                                                                                                                                              ccGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACgg  >  1:423049/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
CCACGGCACTGATGGTCGGGTTATGACGTAAAAGCGCCGTGATAGCTTCCGCGGCTTGCTTCTGGCTGGAAGTGCACTCCAACACCCAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCACGG  >  NC_000913/1698439‑1698728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: