Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,789,497 T→G 5.8% intergenic (+16/‑116) aroH → / → ydiE 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible/hemin uptake protein HemP homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,789,4970TG 5.8% 266.7 / 7.4 87intergenic (+16/‑116)aroH/ydiE3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible/hemin uptake protein HemP homolog
Reads supporting (aligned to +/- strand):  ref base T (39/43);  new base G (3/2);  total (42/45)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

AGGAACGCAAAAAATCGTCGGCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCT  >  NC_000913/1789352‑1789645
                                                                                                                                                 |                                                                                                                                                    
aGGAACGCAAAAAATCGTCGGCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGAc                                                                                                                                                   <  1:238840/149‑1 (MQ=255)
 ggAACGCAAAAAATCGTCGGCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACg                                                                                                                                                  >  8:296963/1‑149 (MQ=255)
 ggAACGCAAAAAATCGTCGGCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACg                                                                                                                                                  <  1:98071/149‑1 (MQ=255)
    aCGCAAAAAATCGTCGGCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGaa                                                                                                                                               >  4:193582/1‑149 (MQ=255)
            aaTCGTCGGCAGTCAGCCGCTCACTTACGGTCACTCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCAtt                                                                                                                                       >  1:97051/1‑149 (MQ=255)
              tcgtcgTCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCGTCAGGAATGGGCac                                                                                                                                        <  8:199579/146‑2 (MQ=255)
              tcgtcgGCAGTCCGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACACGCTTCTGAATGCGTGCCCATTCCGTCAGGAATGGGCacga                                                                                                                                      >  7:199579/1‑145 (MQ=255)
                gtcgGCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCt                                                                                                                                    <  8:130888/148‑1 (MQ=255)
                 tcgGCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCTGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTgc                                                                                                                                  <  3:24407/149‑1 (MQ=255)
                    gCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTgcgc                                                                                                                                >  5:172697/1‑148 (MQ=255)
                           gCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACttgtt                                                                                                                        <  1:120095/149‑1 (MQ=255)
                           gCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACttgtt                                                                                                                        <  3:281481/149‑1 (MQ=255)
                           gCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACttgtt                                                                                                                        >  6:11842/1‑149 (MQ=255)
                           gCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCGGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACttgtt                                                                                                                        <  2:277115/149‑1 (MQ=255)
                              gCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGtc                                                                                                                     <  3:193582/149‑1 (MQ=255)
                              gCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGtc                                                                                                                     >  2:150884/1‑149 (MQ=255)
                                tCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGCACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTACTTACGGAATGGGCATTTCTGAGCAACTTGTTGtctt                                                                                                                   >  2:318784/1‑149 (MQ=255)
                                  aCTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGtcttct                                                                                                                 >  1:385325/1‑149 (MQ=255)
                                    ttACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATgg                                                                                                                                            >  3:410268/1‑120 (MQ=255)
                                    ttACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACttgtt                                                                                                                        <  1:240811/140‑1 (MQ=255)
                                    ttACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACttgtt                                                                                                                        >  2:240811/1‑140 (MQ=255)
                                    ttACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTtctc                                                                                                                >  7:14985/1‑148 (MQ=255)
                                    ttACGGGCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATgg                                                                                                                                            <  4:410268/120‑1 (MQ=255)
                                         gTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACaaa                                                                                                          >  3:232228/1‑149 (MQ=255)
                                            aaTCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAATAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTa                                                                                                       <  2:12175/149‑1 (MQ=255)
                                            aaTCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTa                                                                                                       <  6:348373/149‑1 (MQ=255)
                                            aaTCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTa                                                                                                       >  2:107447/1‑149 (MQ=255)
                                              tCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACt                                                                                                     <  2:28527/149‑1 (MQ=255)
                                               ccATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACtg                                                                                                    >  1:262442/1‑149 (MQ=255)
                                                 aTTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACtgct                                                                                                  >  5:282787/1‑149 (MQ=255)
                                                      cGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGctc                                                                                             <  1:46765/149‑1 (MQ=255)
                                                        aCCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGtctt                                                                                                                   <  7:54437/125‑1 (MQ=255)
                                                        aCCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGtctt                                                                                                                   >  8:54437/1‑125 (MQ=255)
                                                            gtgtCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCa                                                                                       <  6:282787/149‑1 (MQ=255)
                                                            gtgtCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAAAGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGt                                                                                         >  4:393332/1‑147 (MQ=255)
                                                               tCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGc                                                                                     <  8:14985/148‑1 (MQ=255)
                                                                 tgggctgggAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGCCGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCa                                                                                   >  6:332787/1‑148 (MQ=255)
                                                                 tgggctgggAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCa                                                                                   >  5:410668/1‑148 (MQ=255)
                                                                   ggctgggAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCataa                                                                                <  3:295952/149‑1 (MQ=255)
                                                                    gctgggAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAAAGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCataat                                                                               <  3:393332/149‑1 (MQ=255)
                                                                     ctgggAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGGGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCataata                                                                              <  4:232228/149‑1 (MQ=255)
                                                                        ggaggaTACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTATATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATAtt                                                                            <  1:171325/148‑1 (MQ=255)
                                                                        ggaggaTACCGAACGCCTGGTCGAAAAACTCGCCTCTGAGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAatat                                                                             <  5:370520/147‑1 (MQ=255)
                                                                           ggaTACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGata                                                                        <  2:386052/149‑1 (MQ=255)
                                                                            gaTACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGataa                                                                       <  2:262442/149‑1 (MQ=255)
                                                                              tACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGataat                                                                      >  5:331311/1‑148 (MQ=255)
                                                                                ccGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACATATTACTGCTTGCTCTGGTCAGCCATAATATTGAtaataa                                                                    <  6:172697/148‑1 (MQ=255)
                                                                                 cGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGa                                                                  <  2:361777/149‑1 (MQ=255)
                                                                                 cGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGa                                                                  <  1:150884/149‑1 (MQ=255)
                                                                                       ccTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCAtt                                                            <  2:97051/149‑1 (MQ=255)
                                                                                            tCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTta                                                        >  7:355507/1‑148 (MQ=255)
                                                                                              gAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTtata                                                      >  7:46261/1‑148 (MQ=255)
                                                                                              gAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTatat                                                     >  5:323406/1‑149 (MQ=255)
                                                                                              gAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGAATTTCTGCGCACCTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGGCAGCCATAATATTGATAATAAGAATCATTGt                                                          >  7:396242/1‑144 (MQ=255)
                                                                                                  aaCTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAat                                                 <  2:385325/149‑1 (MQ=255)
                                                                                                    cTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAatta                                               >  2:14790/1‑149 (MQ=255)
                                                                                                     tCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAattat                                              >  4:222826/1‑149 (MQ=255)
                                                                                                       gCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAattatta                                            <  5:11842/149‑1 (MQ=255)
                                                                                                          tctGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCTCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAattattatt                                          >  4:297962/1‑148 (MQ=255)
                                                                                                                gTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAAttttt                                   <  6:331311/149‑1 (MQ=255)
                                                                                                                gTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAAttttt                                   <  3:297962/149‑1 (MQ=255)
                                                                                                                     tACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCg                              >  7:317576/1‑149 (MQ=255)
                                                                                                                       cccGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCGTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGt                             >  2:266825/1‑148 (MQ=255)
                                                                                                                        ccGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTta                           >  8:422807/1‑149 (MQ=255)
                                                                                                                        ccGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTta                           >  8:455842/1‑149 (MQ=255)
                                                                                                                          gCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAatt                                                >  1:136792/1‑126 (MQ=255)
                                                                                                                          gCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAatt                                                <  2:136792/126‑1 (MQ=255)
                                                                                                                           cTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTg                                                                                           >  5:292255/1‑82 (MQ=255)
                                                                                                                           cTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTg                                                                                           <  6:292255/82‑1 (MQ=255)
                                                                                                                           cTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATAc                        <  5:332787/149‑1 (MQ=255)
                                                                                                                           cTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATAc                        <  1:107447/149‑1 (MQ=255)
                                                                                                                                gcagaaatGCCCATTCCGTCAGGAATGGGCACTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGa                     >  2:447558/8‑147 (MQ=255)
                                                                                                                                gAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAatt                                                >  7:186664/1‑120 (MQ=255)
                                                                                                                                gAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAatt                                                <  8:186664/120‑1 (MQ=255)
                                                                                                                                      gTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGa              >  2:374904/1‑148 (MQ=255)
                                                                                                                                      atGCCCATTCCGTCAGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAg                  >  6:361160/2‑144 (MQ=255)
                                                                                                                                      atGCCCATTCCGTCAGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAg                  <  5:361160/143‑1 (MQ=255)
                                                                                                                                         cccATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAg                                                                   >  7:227177/1‑92 (MQ=255)
                                                                                                                                         cccATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAg                                                                   <  8:227177/92‑1 (MQ=255)
                                                                                                                                         cccATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGaaaa           <  1:374904/148‑1 (MQ=255)
                                                                                                                                         cccATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTCTATGCGTTATACGGATAGCAGaaa            <  3:405506/147‑1 (MQ=255)
                                                                                                                                         cccATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTCTATGCGTTATACGGATAGCAGaaa            >  4:405506/1‑147 (MQ=255)
                                                                                                                                          ccATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACt         >  5:60270/1‑149 (MQ=255)
                                                                                                                                          ccATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACt         >  2:139077/1‑149 (MQ=255)
                                                                                                                                             ttCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCAc      <  1:14790/149‑1 (MQ=255)
                                                                                                                                                cTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGc    <  7:296963/148‑1 (MQ=255)
                                                                                                                                                cTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGATTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGc    <  1:318784/148‑1 (MQ=255)
                                                                                                                                                 tGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCt  >  3:64546/1‑149 (MQ=255)
                                                                                                                                                 tGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCt  >  7:212870/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
AGGAACGCAAAAAATCGTCGGCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCT  >  NC_000913/1789352‑1789645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑