Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,796,340 G→A 28.3% S535S (AGC→AGT pheT ← phenylalanine tRNA synthetase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,796,3400GA28.3% 143.9 / 64.2 93S535S (AGC→AGTpheTphenylalanine tRNA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (32/34);  new base A (14/12);  total (47/46)
Fisher's exact test for biased strand distribution p-value = 8.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGCCTGAGTATCTGGTACGAAACGCAGACCGCTTTCGAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCTTGAGCGACAGGTCAGCTTCACGGTGA  >  NC_000913/1796197‑1796484
                                                                                                                                               |                                                                                                                                                
ggggCCTGAGTATCTGGTACGAAACGCAGACCGCTTTCGAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTgg                                                                                                                                             <  5:336441/146‑1 (MQ=255)
ggTGCCTGAGTATCTGGTACGAAACGCAGACCGCTTTCGAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTgg                                                                                                                                             <  7:38288/149‑1 (MQ=255)
  tGCCTGAGTATCTGGTACGAAACGCAGACCGCTTTCGAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGACTTGgca                                                                                                                                           <  6:148325/149‑1 (MQ=255)
    ccTGAGTATCTGGTACGAAACGCAGACCGCTTTCGAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGACTTGgcag                                                                                                                                          >  2:363653/1‑148 (MQ=255)
         gTATCTGGTACGAAACGCAGACCGCTTTCGAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTaa                                                                                                                                    <  6:128821/149‑1 (MQ=255)
            tCTGGTACGAAACGCAGACCGCTTTCGAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGc                                                                                                                                 >  7:11025/1‑149 (MQ=255)
              tGGTACGAAACGCAGACCGCTTTCGAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGACTTGGCAGCAGTAAGGCtt                                                                                                                               >  3:261469/1‑149 (MQ=255)
                    gAAACGCAGACCGCTTTCGAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGc                                                                                                                         >  1:169617/1‑149 (MQ=255)
                      aaCGCAGACCGCTTTCGAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCt                                                                                                                       >  6:285889/1‑149 (MQ=255)
                       aCGCAGACCGCTTTCGAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCtg                                                                                                                      >  3:40184/1‑149 (MQ=255)
                       aCGCAGACCGCTTTCGAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGACTTGGCAGCAGTAAGGCTTCAACGCCtg                                                                                                                      >  2:387206/1‑149 (MQ=255)
                         gCAGACCGCTTTCGAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCtgga                                                                                                                    >  6:289002/1‑149 (MQ=255)
                            gACCGCTTTCGAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCtggatg                                                                                                                  >  3:262899/1‑148 (MQ=255)
                             aCCGCTTTCGAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCtggatgga                                                                                                                <  8:73572/149‑1 (MQ=255)
                               cGCTTTCGAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCt                                                                                                                       >  6:330792/1‑140 (MQ=255)
                               cGCTTTCGAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCt                                                                                                                       <  5:330792/140‑1 (MQ=255)
                               cGCTTTCGAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGatc                                                                                                              <  2:230035/149‑1 (MQ=255)
                               cGCTTTCGAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGatc                                                                                                              <  8:11025/149‑1 (MQ=255)
                                  tttCGAAAAAGCGCACAACGTTCAGCTGCCGCTTCTGGTTGTACACAAAGTTTTCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCTGTCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGCatcatc                                                                                                           >  2:413312/1‑149 (MQ=255)
                                    tCGAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATctg                                                                                                         >  3:367223/1‑149 (MQ=255)
                                      gAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCCTTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATctgct                                                                                                       >  5:344808/1‑149 (MQ=255)
                                       aaaaTGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATctgctg                                                                                                      >  8:264650/1‑149 (MQ=255)
                                       aaaaTGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATATCAACAGAGATCGCGCTAGGCAGCAGAAAGGCTTCAACGCCTGGATGGATCATcagctg                                                                                                      >  1:76560/1‑149 (MQ=255)
                                             cgcACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACttt                                                                                                <  7:264650/149‑1 (MQ=255)
                                             cgcACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACttt                                                                                                <  7:186044/149‑1 (MQ=255)
                                              gcACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTc                                                                                               <  4:367223/149‑1 (MQ=255)
                                                 cacGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTc                                                                                               <  7:426696/146‑1 (MQ=255)
                                                 cacGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTc                                                                                               >  8:426696/1‑146 (MQ=255)
                                                  acGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGAt                                                                                           >  8:457599/1‑149 (MQ=255)
                                                     gTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCaa                                                                                        <  4:262899/149‑1 (MQ=255)
                                                           ctgACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGaa                                                                                    <  1:455539/147‑1 (MQ=255)
                                                                ggTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAg                                                                             <  7:465671/149‑1 (MQ=255)
                                                                ggTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAg                                                                             >  7:329461/1‑149 (MQ=255)
                                                                  ttCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGt                                                                           <  3:357019/149‑1 (MQ=255)
                                                                      ggTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTGAACAGAGATCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGAt                                                                        >  5:413674/1‑148 (MQ=255)
                                                                       gTTGTACACCACGGTTGCCCGCAGGCCAGTCCACAGCGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATc                                                                       <  2:149820/148‑1 (MQ=255)
                                                                       gTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGCGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATc                                                                       <  4:40184/148‑1 (MQ=255)
                                                                       gTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGa                                                                         <  5:289002/146‑1 (MQ=255)
                                                                       gTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCa                                                                      >  2:8056/1‑149 (MQ=255)
                                                                        ttGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCtggat                                                                                                                   >  3:154353/1‑103 (MQ=255)
                                                                        ttGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCtggat                                                                                                                   <  4:154353/103‑1 (MQ=255)
                                                                          gTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACtt                                                                   >  6:48008/1‑149 (MQ=255)
                                                                           tACACCACGGTTGCCAGCAGGCCAGTCCAGAGAGAAAGTCGCTTTGCTGACATTTCAACAGTGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGTTGCTGTAGGTGATCACtt                                                                   <  2:76560/148‑1 (MQ=255)
                                                                             caccacGGTTGCCAGCAGGCCTGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCt                                                                <  1:413312/149‑1 (MQ=255)
                                                                             caccacGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCt                                                                >  2:136775/1‑149 (MQ=255)
                                                                              accacGGTTGCCAGCAGGCGAGTCCACAGAGAAAGACGCTTTGCTGACATTTCAACAGAGATCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGAAGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCt                                                                >  5:90278/1‑148 (MQ=255)
                                                                              accacGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTcc                                                                 <  2:230629/147‑1 (MQ=255)
                                                                              accacGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTcc                                                                 >  1:230629/1‑147 (MQ=255)
                                                                              accacGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCt                                                                >  5:171552/1‑148 (MQ=255)
                                                                               ccacGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCaa                                                                                        >  8:181537/1‑123 (MQ=255)
                                                                               ccacGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCaa                                                                                        <  7:181537/123‑1 (MQ=255)
                                                                                cacGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGa                                                              >  4:74907/1‑148 (MQ=255)
                                                                                 acGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGa                                                                         <  6:59230/136‑1 (MQ=255)
                                                                                 acGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGa                                                                         >  5:59230/1‑136 (MQ=255)
                                                                                 acGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATa                                                            <  4:261469/149‑1 (MQ=255)
                                                                                        ccAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCttt                                                      >  7:274775/1‑148 (MQ=255)
                                                                                        ccAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCttt                                                      <  8:274775/148‑1 (MQ=255)
                                                                                          agcagGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTc                                                   <  5:285889/149‑1 (MQ=255)
                                                                                          agcagGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTc                                                   >  1:307593/1‑149 (MQ=255)
                                                                                             agGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGtt                                                <  3:5635/149‑1 (MQ=255)
                                                                                               gCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGtt                                                >  8:462219/1‑147 (MQ=255)
                                                                                               gCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGa                                              <  8:329461/149‑1 (MQ=255)
                                                                                                ccAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGag                                             <  2:307593/149‑1 (MQ=255)
                                                                                                       acaGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGtt                                      >  5:183605/1‑149 (MQ=255)
                                                                                                       acaGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGagcag                                          <  1:35221/145‑1 (MQ=255)
                                                                                                       acaGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGagcag                                          >  2:35221/1‑145 (MQ=255)
                                                                                                         agagAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGtttt                                    >  8:377841/1‑149 (MQ=255)
                                                                                                         agagAAAGACGCATTGCTGACATTTCAACAGAGATCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGtttt                                    <  1:363653/149‑1 (MQ=255)
                                                                                                           agaAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCa                                  >  1:28023/1‑149 (MQ=255)
                                                                                                           agaAAGACGCATTGCTGACATTTCAACAGAGATCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCa                                  >  5:349013/1‑149 (MQ=255)
                                                                                                            gaAAGACGCATTGCTGACATTTCAACAGAGATCTGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGAGCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCAc                                 <  6:341321/149‑1 (MQ=255)
                                                                                                             aaaGACGCATTGCTGACATTTCAACAGAGATCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCAcg                                >  7:305799/1‑149 (MQ=255)
                                                                                                                gACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACgcgc                             <  7:377841/149‑1 (MQ=255)
                                                                                                                   gCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCtt                           >  4:262203/1‑148 (MQ=255)
                                                                                                                    cATTGCTGACATTTCAACAGAGATCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCtt                           >  7:304048/1‑147 (MQ=255)
                                                                                                                     aTTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCTTGAg                        <  5:48008/149‑1 (MQ=255)
                                                                                                                      ttGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCTTGAGc                       <  7:462219/149‑1 (MQ=255)
                                                                                                                         cTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCTTGAGCGa                     >  4:150078/1‑148 (MQ=255)
                                                                                                                          tGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCTTGAGCGa                     >  7:385977/1‑147 (MQ=255)
                                                                                                                           gACATTTCAACAGAGATCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCTTGAGCGACAg                  <  1:387206/149‑1 (MQ=255)
                                                                                                                              aTTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCTTGAGCGACAGGt                >  2:86727/1‑148 (MQ=255)
                                                                                                                                ttCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCTTGAGCGACa                   >  4:45169/1‑143 (MQ=255)
                                                                                                                                ttCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCTTGAGCGACa                   <  3:45169/143‑1 (MQ=255)
                                                                                                                                 tCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCTTGAGCGACAGGTCAGc            >  6:394401/1‑149 (MQ=255)
                                                                                                                                   aaCAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCTTGAGCGACAGGTCAGCtt          >  7:424769/1‑149 (MQ=255)
                                                                                                                                   aaCAGAGATCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCTTGAGCGACAGGTCAGCtt          >  1:7516/1‑149 (MQ=255)
                                                                                                                                     cAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCTTGAGCGACAGGTCAGCTTCa        <  6:183605/149‑1 (MQ=255)
                                                                                                                                     cAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCTTGAGCGACAGGTCAGCTTCa        <  3:150078/149‑1 (MQ=255)
                                                                                                                                     cAGAGATCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCTTGAGCGACAGGTCAGCTTCa        <  1:136775/149‑1 (MQ=255)
                                                                                                                                      agagATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCTTGAGCGACAGGTCAGCTTCAc       <  2:28023/149‑1 (MQ=255)
                                                                                                                                       gagaTCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCTTGAGCGACAGGTCAGCTTCACg      <  6:344808/149‑1 (MQ=255)
                                                                                                                                         gaTCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCTTGAGCGACAGGTCAGCTTCACGGt    <  1:8056/149‑1 (MQ=255)
                                                                                                                                           tCGGACTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCTTGAGCGACAGGTCAGCTTCACGGTGa  <  8:305799/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GGTGCCTGAGTATCTGGTACGAAACGCAGACCGCTTTCGAAAATGCGCACACGGTTCTGCTGACGGTTCTGGTTGTACACCACGGTTGCCAGCAGGCCAGTCCACAGAGAAAGACGCATTGCTGACATTTCAACAGAGATCGGGCTTGGCAGCAGTAAGGCTTCAACGCCTGGATGGATCATCTGCTGCACTTTCGGATCAACGAAGCTGTAGGTGATCACTTCCTGATAGCCTTTGTCGTTGAGCAGCGTTTTCACGCGCTTGAGCGACAGGTCAGCTTCACGGTGA  >  NC_000913/1796197‑1796484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: