Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,159,287 C→A Y473* (TAC→TAA ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,159,2870CA100.0% 146.9 / NA 42Y473* (TAC→TAAptsGfused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (20/22);  total (20/22)

TGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGT  >  NC_000913/1159142‑1159429
                                                                                                                                                 |                                                                                                                                              
tGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGTCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTACATCCGATAACCTGAAAACCGAGATGGATGAGTAAATc                                                                                                                                             >  3:36807/1‑149 (MQ=255)
  cgcgTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATcc                                                                                                                                            <  6:64215/148‑1 (MQ=255)
     ggcAGCGTTGCTGATGTGTCTAAAGTGTATCAGGCCTGCCTGAAGATCCTGGGCGCAGCGGGCGTAGTGGGTGCTGGTTCTGGTGTTCAGGCGATTTTCGTTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCAGTaa                                                                                                                                        <  4:36807/147‑1 (MQ=255)
                tGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCg                                                                                                                             >  8:32806/1‑149 (MQ=255)
                tGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCg                                                                                                                             >  3:110540/1‑149 (MQ=255)
                           aaGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGtt                                                                                                                    <  1:38979/147‑1 (MQ=255)
                             gTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTggg                                                                                                                 >  1:112918/1‑148 (MQ=255)
                                gATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGgaga                                                                                                             <  4:1971/149‑1 (MQ=255)
                                     ggccggccTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAg                                                                                                        <  4:110540/149‑1 (MQ=255)
                                           ccTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATTGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGcc                                                                                                  <  8:91700/149‑1 (MQ=255)
                                                     cttggCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGc                                                                                        <  2:141118/146‑1 (MQ=255)
                                                         gcgcAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCATCTGGCTGCCtta                                                                                    <  7:32806/149‑2 (MQ=255)
                                                         gcgcAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCATCTGGCTGCCtta                                                                                    <  3:56307/149‑2 (MQ=255)
                                                                    gTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGgtgt                                                                         <  2:112918/149‑1 (MQ=255)
                                                                          gTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTgg                                                                                                                  >  3:113062/1‑102 (MQ=255)
                                                                          gTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTgg                                                                                                                  <  4:113062/102‑1 (MQ=255)
                                                                               tGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAg                                                                             >  8:38679/1‑134 (MQ=255)
                                                                                     tGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACt                                                                                                           <  6:110212/98‑1 (MQ=255)
                                                                                     tGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACt                                                                                                           >  5:110212/1‑98 (MQ=255)
                                                                                             aGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGt                                                 >  2:103646/1‑148 (MQ=255)
                                                                                             aGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGt                                                 >  6:10891/1‑148 (MQ=255)
                                                                                              ggCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTaa                                               >  5:88498/1‑149 (MQ=255)
                                                                                               gCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAAt                                              >  1:170452/1‑149 (MQ=255)
                                                                                               gCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAAt                                              >  8:79204/1‑149 (MQ=255)
                                                                                                            cTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTTAAATTAcg                                  <  2:170452/148‑1 (MQ=255)
                                                                                                             tAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCatc                                                                                               >  2:11090/1‑84 (MQ=255)
                                                                                                                aTCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTg                                                             >  5:4632/1‑117 (MQ=255)
                                                                                                                aTCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTg                                                             <  6:4632/117‑1 (MQ=255)
                                                                                                                    gATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGaa                                                                <  8:127172/110‑1 (MQ=255)
                                                                                                                    gATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGcc                         <  6:88498/149‑1 (MQ=255)
                                                                                                                     aTAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTaa                                               <  5:145250/126‑1 (MQ=255)
                                                                                                                     aTAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTaa                                               >  6:145250/1‑126 (MQ=255)
                                                                                                                         ccTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGAAATGCTGAAATTACGCGGTGTGCCGTAGa                    <  8:102868/149‑1 (MQ=255)
                                                                                                                          cTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTa                                                                       <  2:102665/97‑1 (MQ=255)
                                                                                                                           tGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGAc                   >  3:134975/1‑148 (MQ=255)
                                                                                                                            gAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTAcg                                  <  4:107612/132‑1 (MQ=255)
                                                                                                                            gAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTAcg                                  >  3:107612/1‑132 (MQ=255)
                                                                                                                                 ccGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGaa            <  4:134975/149‑1 (MQ=255)
                                                                                                                                   gagaTGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAcc          >  5:49645/1‑149 (MQ=255)
                                                                                                                                           tgAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGt  >  4:61189/1‑149 (MQ=255)
                                                                                                                                            gAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCt                                                                                          >  6:112757/1‑60 (MQ=255)
                                                                                                                                            gAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCt                                                                                          <  5:112757/60‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGT  >  NC_000913/1159142‑1159429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: