Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,446,362 Δ1 bp 5.4% intergenic (+156/+16) ydbL → / ← feaR DUF1318 family protein/transcriptional activator for tynA and feaB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,446,3620A. 5.4% 309.4 / 9.5 92intergenic (+156/+16)ydbL/feaRDUF1318 family protein/transcriptional activator for tynA and feaB
Reads supporting (aligned to +/- strand):  ref base A (47/40);  new base . (2/3);  total (49/43)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

ACCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTTCATCATCTGCGGCATGG  >  NC_000913/1446214‑1446510
                                                                                                                                                     |                                                                                                                                                    
aCCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACa                                                                                                                                                      <  1:385230/149‑1 (MQ=255)
   gCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGc                                                                                                                                                   <  1:400753/149‑1 (MQ=255)
      atatCGTGATAACGCTACAGTCGAAGGCGATTGATCTATTTTCCTGAAACAATGTTAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAAc                                                                                                                                                <  7:327945/149‑1 (MQ=255)
      atatCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAAc                                                                                                                                                <  8:63880/149‑1 (MQ=255)
      atatCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTCAATATTCAAAAACTCCTGTCAAATTGACTTTTGCCATGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAAc                                                                                                                                                <  7:327949/149‑1 (MQ=255)
       tatCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACg                                                                                                                                               >  4:391266/1‑149 (MQ=255)
        atCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGc                                                                                                                                              >  1:205470/1‑149 (MQ=255)
        atCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGc                                                                                                                                              >  3:388367/1‑149 (MQ=255)
         tCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCTTTGCATGTACTGCAACGc                                                                                                                                              >  3:29074/1‑148 (MQ=255)
         tCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGc                                                                                                                                              <  3:391266/148‑1 (MQ=255)
           gggATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCAGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCaa                                                                                                                                           <  1:419561/149‑1 (MQ=255)
            ggATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAt                                                                                                                                          >  4:214363/1‑149 (MQ=255)
             gATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAtt                                                                                                                                         <  2:395514/149‑1 (MQ=255)
              aTAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAtta                                                                                                                                        <  4:372481/149‑1 (MQ=255)
              aTAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAtta                                                                                                                                        <  3:317339/149‑1 (MQ=255)
                aaCGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttagt                                                                                                                                      <  7:453605/149‑1 (MQ=255)
                   gCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttagttag                                                                                                                                   >  3:379854/1‑149 (MQ=255)
                    cTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGc                                                                                                                                  <  7:325058/149‑1 (MQ=255)
                     tACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCg                                                                                                                                 >  5:87741/1‑149 (MQ=255)
                       cAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCg                                                                                                                                 <  4:265646/147‑1 (MQ=255)
                          gagaAGGCGATTGATCTATTTTCCTGAAACAAGGTGATTATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACAAGCAACGCCAAtta                                                                                                                                        >  2:386267/1‑137 (MQ=255)
                          gagaAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAtta                                                                                                                                        <  1:386267/137‑1 (MQ=255)
                          gagaAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAAtt                                                                                                                            >  7:67795/1‑149 (MQ=255)
                          gagaAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAAtt                                                                                                                            >  4:38690/1‑149 (MQ=255)
                               ggCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGTAATTTACGt                                                                                                                       >  6:35914/1‑149 (MQ=255)
                                gCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTc                                                                                                                      <  3:38690/149‑1 (MQ=255)
                                  gATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttagtta                                                                                                                                    <  3:362948/133‑1 (MQ=255)
                                  gATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttagtta                                                                                                                                    >  4:362948/1‑133 (MQ=255)
                                  gATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGa                                                                                                                    >  2:241996/1‑149 (MQ=255)
                                   aTTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGAt                                                                                                                   <  1:241996/149‑1 (MQ=255)
                                    ttGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATa                                                                                                                  <  3:214363/149‑1 (MQ=255)
                                       aTCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTc                                                                                                               >  2:60247/1‑149 (MQ=255)
                                       aTCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTc                                                                                                               >  2:224740/1‑149 (MQ=255)
                                        tCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCTGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCg                                                                                                              >  4:71884/1‑149 (MQ=255)
                                            ttttCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTg                                                                                                          <  1:60247/149‑1 (MQ=255)
                                                  tGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCa                                                                                                    >  6:73458/1‑149 (MQ=255)
                                                  tGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCa                                                                                                    >  6:257209/1‑149 (MQ=255)
                                                        aaGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCa                                                                                               >  7:378520/1‑148 (MQ=255)
                                                           gTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGc                                                                                           <  6:87741/149‑1 (MQ=255)
                                                                  ttCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCt                                                                                     >  3:91776/1‑148 (MQ=255)
                                                                  ttCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCACAAAGCGTTGCt                                                                                     >  5:278853/1‑148 (MQ=255)
                                                                   tCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCtt                                                                                    >  4:392463/1‑148 (MQ=255)
                                                                   tCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCtt                                                                                    >  5:158121/1‑148 (MQ=255)
                                                                    cAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTa                                                                                   >  5:312685/1‑148 (MQ=255)
                                                                    cAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTa                                                                                   >  5:312682/1‑148 (MQ=255)
                                                                     aaaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTaaa                                                                                 >  2:411359/1‑149 (MQ=255)
                                                                      aaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttagtt                                                                                                                                     >  6:290198/1‑96 (MQ=255)
                                                                      aaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttagtt                                                                                                                                     <  5:290198/96‑1 (MQ=255)
                                                                      aaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTaaa                                                                                 >  6:144885/1‑148 (MQ=255)
                                                                        aaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATAc                                                                              <  1:224740/149‑1 (MQ=255)
                                                                        aaCTCCTGTCAAATTGCCTTTCGCCCTGAAAAATGCATACTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATAc                                                                              <  3:189510/149‑1 (MQ=255)
                                                                          cTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCg                                                                            <  3:392463/149‑1 (MQ=255)
                                                                           tCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGt                                                                           >  5:183757/1‑149 (MQ=255)
                                                                             cTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGtt                                                                          >  3:116393/1‑148 (MQ=255)
                                                                              tGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTg                                                                         >  5:199041/1‑148 (MQ=255)
                                                                                tCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaa                                                                      <  6:312685/149‑1 (MQ=255)
                                                                                tCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaa                                                                      <  6:312682/149‑1 (MQ=255)
                                                                                tCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaa                                                                       >  7:303101/1‑148 (MQ=255)
                                                                                tCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaa                                                                       >  5:250822/1‑148 (MQ=255)
                                                                                tCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGAGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaa                                                                       >  5:369472/1‑148 (MQ=255)
                                                                                  aaaTTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaaaa                                                                    <  5:144885/149‑1 (MQ=255)
                                                                                     ttGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGa                                                                 <  5:73458/149‑1 (MQ=255)
                                                                                        ccTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAct                                                               <  6:369472/148‑1 (MQ=255)
                                                                                        ccTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAct                                                               <  6:250822/148‑1 (MQ=255)
                                                                                        ccTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAACTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGActc                                                              >  5:389000/1‑149 (MQ=255)
                                                                                          ttttGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTg                                                            >  5:337498/1‑149 (MQ=255)
                                                                                           tttGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTgg                                                           >  3:104037/1‑149 (MQ=255)
                                                                                              gCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGt                                                          >  7:353724/1‑147 (MQ=255)
                                                                                               cccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCa                                                        <  8:353724/148‑1 (MQ=255)
                                                                                               cccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAg                                                       <  6:183757/149‑1 (MQ=255)
                                                                                                  tGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGaa                                                     >  5:376346/1‑148 (MQ=255)
                                                                                                       aaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAACTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATccc                                               <  6:389000/149‑1 (MQ=255)
                                                                                                          gCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAATTCCCCaa                                            >  6:260997/1‑149 (MQ=255)
                                                                                                           cATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCaa                                            <  5:35914/148‑1 (MQ=255)
                                                                                                           cATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCaa                                            <  4:91776/148‑1 (MQ=255)
                                                                                                           cATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAAt                                           <  4:388367/149‑1 (MQ=255)
                                                                                                           cATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAAt                                           <  2:205470/149‑1 (MQ=255)
                                                                                                            aTAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATg                                          <  8:303101/149‑1 (MQ=255)
                                                                                                              aGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGa                                         >  6:174602/1‑148 (MQ=255)
                                                                                                                   aaTATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCg                                   <  5:257209/149‑1 (MQ=255)
                                                                                                                     tatCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGAt                                 <  8:67795/149‑1 (MQ=255)
                                                                                                                     tatCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGAt                                 <  4:379854/149‑1 (MQ=255)
                                                                                                                       tCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGaaa                                                    >  8:163938/1‑128 (MQ=255)
                                                                                                                       tCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGAtgc                               >  2:437356/1‑149 (MQ=255)
                                                                                                                       tCTGTTTTAACTAATTGGCGTTGCAGTACATGCAAAGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGaaa                                                    <  7:163938/128‑1 (MQ=255)
                                                                                                                                aCTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGtt                      >  3:327258/1‑149 (MQ=255)
                                                                                                                                  tAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGtttt                    >  7:352466/1‑149 (MQ=255)
                                                                                                                                    aTTGGCGTTGCATGTACTGCAAAGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAAGCCCCACTGCAAGCCGCTGCCGGCCAGttttt                   >  6:309737/1‑148 (MQ=255)
                                                                                                                                    aTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTtc                  <  6:199041/149‑1 (MQ=255)
                                                                                                                                          gTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTTCatcatc            >  7:291708/1‑149 (MQ=255)
                                                                                                                                             gCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGAtg                                <  7:382246/126‑1 (MQ=255)
                                                                                                                                             gCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGAtg                                >  8:382246/1‑126 (MQ=255)
                                                                                                                                                  tACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCTGATGCCTGCCAGTTTTTCATCATCTGCGGCa     >  7:173736/1‑149 (MQ=255)
                                                                                                                                                     aTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTTCATCATCTGCGGCATgg  <  6:337498/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTTCATCATCTGCGGCATGG  >  NC_000913/1446214‑1446510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: