Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,570,610 G→A 5.4% intergenic (‑121/+35) gadC ← / ← gadB glutamate:gamma‑aminobutyric acid antiporter/glutamate decarboxylase B, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,570,6100GA 5.4% 414.1 / 7.5 130intergenic (‑121/+35)gadC/gadBglutamate:gamma‑aminobutyric acid antiporter/glutamate decarboxylase B, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (59/62);  new base A (2/5);  total (62/68)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

CTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATT  >  NC_000913/1570462‑1570756
                                                                                                                                                    |                                                                                                                                                  
ttcgaaatggactttgctgaactgttgctggaagactacaaagcctccctgaaatatctcagcgatcacccgaaactgcagggtattgcccaacagaacagctttaaacatacctgataacgtttaacggtaacggTGTCCCGAAACgg                                                                                                                                                    <  8:102831‑M1/13‑1 (MQ=255)
ttcgaaatggactttgctgaactgttgctggaagactacaaagcctccctgaaatatctcagcgatcacccgaaactgcagggtattgcccaacagaacagctttaaacatacctgataacgtttaacggtaacggTGTCCCGAAACgg                                                                                                                                                    <  8:388920‑M1/13‑1 (MQ=255)
 tttACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACggg                                                                                                                                                   >  4:191480/1‑149 (MQ=255)
  ttACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGt                                                                                                                                                  >  7:100632/1‑149 (MQ=255)
      ctgtctgtACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGt                                                                                                                                              <  6:50786/149‑1 (MQ=255)
         tctgtACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTc                                                                                                                                           >  5:54882/1‑149 (MQ=255)
         tctgtACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTc                                                                                                                                           >  1:411283/1‑149 (MQ=255)
           tgtACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCgg                                                                                                                                         >  6:385542/1‑149 (MQ=255)
           ctttgctgaactgttgctggaagactacaaagcctccctgaaatatctcagcgatcacccgaaactgcagggtattgcccaacagaacagctttaaacatacctgataacgtttaacggtaacggTGTCCCGAAACGGGTTCGTTTCgg                                                                                                                                         >  7:388920‑M1/126‑149 (MQ=255)
           ctttgctgaactgttgctggaagactacaaagcctccctgaaatatctcagcgatcacccgaaactgcagggtattgcccaacagaacagctttaaacatacctgataacgtttaacggtaacggTGTCCCGAAACGGGTTCGTTTCgg                                                                                                                                         >  8:136967‑M1/126‑149 (MQ=255)
            gtACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCgg                                                                                                                                         >  2:315646/1‑148 (MQ=255)
             tACTGATGTATCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCgg                                                                                                                                         <  2:214680/147‑1 (MQ=255)
               cTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCg                                                                                                                                          <  8:458134/144‑1 (MQ=255)
                  aTGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACaccg                                                                                                                                  <  7:28065/149‑1 (MQ=255)
                     tAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGAACCCGTTTCGGGacaatttcc                                                                                                                               <  8:442113/149‑7 (MQ=255)
                     tAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGAACCCGTTTCGGGacaatttc                                                                                                                                <  7:119138/148‑6 (MQ=255)
                        ccATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCg                                                                                                                                          >  7:457894/1‑135 (MQ=255)
                        ccATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACaccgttaccg                                                                                                                            <  2:105682/149‑1 (MQ=255)
                         tatatTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACaccgttaccgt                                                                                                                           <  3:219945/148‑1 (MQ=255)
                           tattatCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACaccgttaccgtt                                                                                                                          >  6:243033/1‑148 (MQ=255)
                           tattatCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACaccgttaccgt                                                                                                                           >  4:219945/1‑147 (MQ=255)
                                tCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGt                                                                                                                    <  3:191480/149‑1 (MQ=255)
                                   cccTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTAt                                                                                                                 >  7:286894/1‑149 (MQ=255)
                                    ccTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTAt                                                                                                                 >  8:168616/1‑148 (MQ=255)
                                    ccTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTAt                                                                                                                 >  7:270927/1‑148 (MQ=255)
                                       aaagcctccctgaaatatctcagcgatcacccgaaactgcagggtattgcccaacagaacagctttaaacatacctgataacgtttaacggtaacggTGTCCCGAAACGGGTTc                                                                                                                                                <  7:343206‑M1/17‑1 (MQ=255)
                                              tATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGtt                                                                                                       >  6:323468/1‑148 (MQ=255)
                                                  ccTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTaaa                                                                                                   <  5:385542/148‑1 (MQ=255)
                                                        ggAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGctgttc                                                                                            >  6:326249/1‑149 (MQ=255)
                                                         gAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGctgttct                                                                                           <  5:323468/149‑1 (MQ=255)
                                                         gAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGctgtt                                                                                             >  7:51702/1‑147 (MQ=255)
                                                          aaCCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGctgttctg                                                                                          >  7:268562/1‑149 (MQ=255)
                                                            ccGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCa                                                                                                               <  8:364051/126‑1 (MQ=255)
                                                             cGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTg                                                                                       <  1:315646/149‑1 (MQ=255)
                                                              gCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATTTTTTCAGTTGCTAATGCCAGTGAACATACTTTGGAAATTGTGACTAAACGTGTTCGTTTCGGGAGACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTgg                                                                                      <  5:244021/149‑1 (MQ=255)
                                                              gCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTgg                                                                                      <  8:100632/149‑1 (MQ=255)
                                                                aCCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGctgttctgt                                                                                         >  6:199247/1‑144 (MQ=255)
                                                                aCCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGctgttctgt                                                                                         <  5:199247/144‑1 (MQ=255)
                                                                aCCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTggg                                                                                     >  3:279847/1‑148 (MQ=255)
                                                                aCCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGc                                                                                    <  8:268562/149‑1 (MQ=255)
                                                                    gTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGctgttctgtt                                                                                        >  4:227135/1‑141 (MQ=255)
                                                                       ttGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTggg                                                                                     >  2:453490/1‑141 (MQ=255)
                                                                            cTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCgg                                                                                                                                         <  4:334991/84‑1 (MQ=255)
                                                                            cTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCgg                                                                                                                                         >  3:334991/1‑84 (MQ=255)
                                                                            cTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCa                                                                                   >  6:219420/1‑138 (MQ=255)
                                                                             tGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGtt                                                                                                       >  1:466781/1‑117 (MQ=255)
                                                                             tGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGt                                                                       >  6:307107/1‑149 (MQ=255)
                                                                                     aTTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACaccgttaccg                                                                                                                            >  8:459747/1‑88 (MQ=255)
                                                                                     aTTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACaccgttaccg                                                                                                                            <  7:459747/88‑1 (MQ=255)
                                                                                      ttCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGa                                                              >  7:154221/1‑149 (MQ=255)
                                                                                        cAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGAt                                                             <  3:417231/148‑1 (MQ=255)
                                                                                        cAGAACAATATTATCCGCTGCTCATGCCCGTCAACAGACGTTGGAAATCGTCCCGAACCGGGGTCCCTTCGGGACACCGTTACCGTACCACGGTATCAGCCATGTTTAAAGCTGCTCTGCCGGCCAATACCCTGCAGCTTCggggcacc                                                            <  4:279847/149‑6 (MQ=255)
                                                                                         aGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCg                                                           >  7:341124/1‑149 (MQ=255)
                                                                                          gAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGc                                                          >  7:425284/1‑149 (MQ=255)
                                                                                           aacagaacagctttaaacatacctgataacgtttaacggtaacggTGTCCCGAAACGAACCCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCt                                                         <  3:202323‑M1/104‑1 (MQ=255)
                                                                                             cAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCg                                                                   >  1:28288/1‑137 (MQ=255)
                                                                                             cAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCg                                                                   <  2:28288/137‑1 (MQ=255)
                                                                                             cAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCt                                                         >  2:75435/1‑147 (MQ=255)
                                                                                             cAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTga                                                       <  7:168616/149‑1 (MQ=255)
                                                                                              aaTATTTTCCGTTGCTAATGACAGTGAACAGACTTTGGAAATTGTCCCGAAACGCGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGt                                                                                                        >  4:373527/1‑99 (MQ=255)
                                                                                              aaTATTTTCCGTTGATAATGCCAGTGAAGATACTTTGGATATTGTCCTGTATCGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGt                                                                                                        <  3:373527/99‑1 (MQ=255)
                                                                                                     ctttaaacatacctgataacgtttaacggtaacggTGTCCCGAAACGAACCCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATAtt                                                 >  8:464767‑M1/36‑147 (MQ=255)
                                                                                                     ctttaaacatacctgataacgtttaacggtaacggTGTCCCGAAACGAACCCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATAtt                                                 <  7:464767‑M1/112‑1 (MQ=255)
                                                                                                       cGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCa                                              <  8:341124/148‑1 (MQ=255)
                                                                                                       cGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCa                                              <  8:214480/148‑1 (MQ=255)
                                                                                                       cGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCa                                              <  6:54882/148‑1 (MQ=255)
                                                                                                        gTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTaaa                                                                                                   <  4:85872/94‑1 (MQ=255)
                                                                                                        gTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTaaa                                                                                                   >  3:85872/1‑94 (MQ=255)
                                                                                                        gTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAgg                                            >  2:467913/1‑149 (MQ=255)
                                                                                                        gTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAgg                                            <  5:243033/149‑1 (MQ=255)
                                                                                                        gTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGGTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAgg                                            <  5:366076/149‑1 (MQ=255)
                                                                                                             tAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCCTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGAGCTCTGAGATATTTCAGGGAGGc                                       >  8:354598/1‑149 (MQ=255)
                                                                                                               aTGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCt                                      <  8:96297/148‑1 (MQ=255)
                                                                                                                   cAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTg                                   >  2:41998/1‑147 (MQ=255)
                                                                                                                   cAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTa                                 <  1:467913/149‑1 (MQ=255)
                                                                                                                    aGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAAt                                                                                 <  6:374449/100‑1 (MQ=255)
                                                                                                                     gTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACTGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTggg                                                                                     <  5:341685/95‑1 (MQ=255)
                                                                                                                     gTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACTGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTggg                                                                                     >  6:341685/1‑95 (MQ=255)
                                                                                                                     gTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTga                                                       <  3:61376/125‑1 (MQ=255)
                                                                                                                     gTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTga                                                       >  4:61376/1‑125 (MQ=255)
                                                                                                                          cAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTga                                                       >  3:237333/1‑120 (MQ=255)
                                                                                                                          cAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCa                                              <  1:237579/129‑1 (MQ=255)
                                                                                                                          cAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCa                                              >  2:237579/1‑129 (MQ=255)
                                                                                                                           aGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCGGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTTAGATATTTCAGGGAGGCTTTGTAGTCTTccc                         >  5:25181/1‑148 (MQ=255)
                                                                                                                           aGACTTTGGAAATTGTCACGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCa                         <  5:307107/149‑1 (MQ=255)
                                                                                                                                 tGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCaa                     <  1:75435/147‑1 (MQ=255)
                                                                                                                                   gAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGt                 >  3:210136/1‑149 (MQ=255)
                                                                                                                                    acggTGTCCCGAAACGAACCCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGtt                >  4:202323‑M1/5‑149 (MQ=255)
                                                                                                                                    aaaTTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATc                                                            <  2:383994/105‑1 (MQ=255)
                                                                                                                                      aTTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCa              <  8:286894/149‑1 (MQ=255)
                                                                                                                                      aTTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCa              <  1:41998/149‑1 (MQ=255)
                                                                                                                                          TCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTg                                   >  8:282006‑M1/1‑124 (MQ=255)
                                                                                                                                          TCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCa           <  8:438320‑M1/148‑1 (MQ=255)
                                                                                                                                            CCGAAACTGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCtt                                     >  1:429271‑M1/1‑120 (MQ=255)
                                                                                                                                            CCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCa                         <  8:455757‑M1/132‑1 (MQ=255)
                                                                                                                                            CCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGAAACAGTTCAGCAAAg        <  8:77450‑M1/149‑1 (MQ=255)
                                                                                                                                            CCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCATCCAGCAACAGTTCAGCaaa         >  3:372135‑M1/1‑148 (MQ=255)
                                                                                                                                            CCGAAACGGGTTCGTTTCGGGACACAGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGAAGTTTCGGGTGATCTCTGAGATATTTCAGGTAGGcta                                     <  2:429271‑M1/120‑2 (MQ=255)
                                                                                                                                              GAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTc      <  5:440224‑M1/149‑1 (MQ=255)
                                                                                                                                                  CGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCAtt  >  5:326249‑M1/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATT  >  NC_000913/1570462‑1570756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: