Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,649,718 A→G 89.1% E37G (GAA→GGA)  dicB → Qin prophage; cell division inhibition protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,649,7180AG89.1% 310.1 / 21.9 112E37G (GAA→GGA) dicBQin prophage; cell division inhibition protein
Reads supporting (aligned to +/- strand):  ref base A (5/7);  new base G (49/50);  total (54/58)
Fisher's exact test for biased strand distribution p-value = 7.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 112 total aligned reads displayed.

ACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGT  >  NC_000913/1649574‑1649861
                                                                                                                                                |                                                                                                                                               
aCTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACgg                                                                                                                                             >  7:387853/1‑149 (MQ=255)
  tGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACgg                                                                                                                                             >  1:147659/1‑147 (MQ=255)
    cATCACAAACTTCATTGTTGAGGACGCTATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGAAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGc                                                                                                                                         <  6:277130/149‑1 (MQ=255)
    cATCACAAAATTCCTTGTTGAGGCCGCGATAATGAAAACGTTAATACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTAGCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGCAGc                                                                                                                                         <  4:102651/149‑1 (MQ=255)
    cATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGc                                                                                                                                         <  8:387853/149‑1 (MQ=255)
        acaAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTAtt                                                                                                                                     <  3:392201/149‑1 (MQ=255)
          aaaaTTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATta                                                                                                                                    >  2:378160/1‑148 (MQ=255)
           aaaTTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATtaaa                                                                                                                                  <  8:166348/149‑1 (MQ=255)
           aaaTTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATtaaa                                                                                                                                  <  8:414314/149‑1 (MQ=255)
                 aTTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTaaataaaa                                                                                                                             <  7:18540/148‑1 (MQ=255)
                 aTTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTaaataaaa                                                                                                                             <  2:117407/148‑1 (MQ=255)
                  ttgttgAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTaaataaaata                                                                                                                           >  4:337980/1‑149 (MQ=255)
                     ttgAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGc                                                                                                                        <  2:147659/149‑1 (MQ=255)
                      tgAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCa                                                                                                                       >  7:212596/1‑149 (MQ=255)
                          gACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGt                                                                                                                   >  1:42241/1‑149 (MQ=255)
                          gACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGt                                                                                                                   >  8:176569/1‑149 (MQ=255)
                               gATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCaa                                                                                                              >  8:174399/1‑149 (MQ=255)
                               gATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCaa                                                                                                              >  6:212399/1‑149 (MQ=255)
                                aTAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAAt                                                                                                             >  2:305134/1‑149 (MQ=255)
                                 tAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATg                                                                                                            <  8:288963/149‑1 (MQ=255)
                                  aaTGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGc                                                                                                           >  1:178457/1‑149 (MQ=255)
                                   aTGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGc                                                                                                           <  1:37246/148‑1 (MQ=255)
                                      aaaaCGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTgg                                                                                                        <  4:395189/148‑1 (MQ=255)
                                          cGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGt                                                                                                   <  2:42241/149‑1 (MQ=255)
                                           gTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGtt                                                                                                  >  3:38097/1‑149 (MQ=255)
                                                  ccAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTATCCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCAt                                                                                                                      >  2:73163/1‑122 (MQ=255)
                                                  ccAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTATCCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCAt                                                                                                                      <  1:73163/122‑1 (MQ=255)
                                                  ccAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCt                                                                                           >  1:348617/1‑149 (MQ=255)
                                                  ccAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGAAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATCCATTGTTTCAATGCTGGCTCGTTTACGTCt                                                                                           >  6:304362/1‑149 (MQ=255)
                                                     aaCGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGAt                                                                                        >  3:19228/1‑149 (MQ=255)
                                                        gTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGc                                                                                      >  7:118060/1‑148 (MQ=255)
                                                        gTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACAATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGc                                                                                      >  2:109293/1‑148 (MQ=255)
                                                            aTACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCaaa                                                                                  <  8:103107/148‑1 (MQ=255)
                                                              aCGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAg                                                                                >  4:419733/1‑148 (MQ=255)
                                                               cGTTTGAAGGTTGTTTTGAAATTGGTGTCACTATGAGTAACCCAGTATTTACTGAATATGCGATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCTTTGTTTCAATGCTGGCTCGTTTAGGTCTGATGCCAAAAgg                                                                               <  5:304362/148‑1 (MQ=255)
                                                                 tCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGAtg                                                                            <  1:378160/149‑1 (MQ=255)
                                                                   tGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGAtgtg                                                                          >  8:201664/1‑149 (MQ=255)
                                                                    gAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGAtgtg                                                                          >  8:434338/1‑148 (MQ=255)
                                                                     aaGGTTGTTTTTAAATGTGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGAtgt                                                                           <  4:258196/146‑1 (MQ=255)
                                                                     aaGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGAtgt                                                                           <  1:122551/146‑1 (MQ=255)
                                                                       ggTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGcac                                                                       >  4:139643/1‑148 (MQ=255)
                                                                       ggTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATACGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGcac                                                                       >  6:382417/1‑148 (MQ=255)
                                                                        gttgttTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACaa                                                                     <  4:351337/149‑1 (MQ=255)
                                                                            ttttGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGaa                                                                 >  2:13395/1‑149 (MQ=255)
                                                                            ttttGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGaa                                                                 >  2:367754/1‑149 (MQ=255)
                                                                                 aaaTTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAAtt                                                               <  7:234920/146‑1 (MQ=255)
                                                                                 aaaTTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAAtt                                                               >  8:234920/1‑146 (MQ=255)
                                                                                  aaTTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAg                                                            <  7:174399/148‑1 (MQ=255)
                                                                                   aTTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCa                                                          >  1:197766/1‑149 (MQ=255)
                                                                                    ttGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGc                                                           >  6:13325/1‑147 (MQ=255)
                                                                                    ttGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGc                                                           <  5:13325/147‑1 (MQ=255)
                                                                                       gtgtCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCAttt                                                       <  4:19228/148‑1 (MQ=255)
                                                                                       gtgtCACAATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCAttt                                                       <  1:109293/148‑1 (MQ=255)
                                                                                        tgtCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTtgt                                                     <  3:337980/149‑1 (MQ=255)
                                                                                          tCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGc                                                   >  6:336670/1‑149 (MQ=255)
                                                                                            aCTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATtaaataaa                                                                                                                              <  7:86318/72‑1 (MQ=255)
                                                                                             cTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTg                                                >  3:150541/1‑149 (MQ=255)
                                                                                              tATCAGTATCCCAGTAATTACTGAAGATGCCATTATCAAGAGAAAACAAGCACGGGAGCTATTAAATAAAATATGCTTTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCtt                                                 <  3:304617/147‑1 (MQ=255)
                                                                                                tCAGTAACCCATTATTTACTGATGATGAGATTAACCAGAGAAACCAAGGACGGGAGCTATTAAATAAAATATGATTTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTc                                             <  3:362568/149‑1 (MQ=255)
                                                                                                tCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTc                                             >  5:296995/1‑149 (MQ=255)
                                                                                                tCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTc                                             >  7:433975/1‑149 (MQ=255)
                                                                                                  aGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCaaaa                                                                                 >  1:163345/1‑111 (MQ=255)
                                                                                                  aGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCaaaa                                                                                 <  2:163345/111‑1 (MQ=255)
                                                                                                  aGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTtgt                                                     <  7:193495/139‑1 (MQ=255)
                                                                                                  aGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTtgt                                                     <  7:199898/139‑1 (MQ=255)
                                                                                                  aGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTtgt                                                     >  8:193495/1‑139 (MQ=255)
                                                                                                  aGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTtgt                                                     >  8:199898/1‑139 (MQ=255)
                                                                                                  aGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCt                                            <  8:212596/148‑1 (MQ=255)
                                                                                                    tAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTAc                                                                                               <  6:263828/95‑1 (MQ=255)
                                                                                                    tAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTAc                                                                                               >  5:263828/1‑95 (MQ=255)
                                                                                                      aCCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGc                                                                                                           <  4:85913/81‑1 (MQ=255)
                                                                                                      aCCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGc                                                                                                           >  3:85913/1‑81 (MQ=255)
                                                                                                      aCCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAg                                       >  5:371163/1‑149 (MQ=255)
                                                                                                            tATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTc                                 <  5:212399/149‑1 (MQ=255)
                                                                                                              tttACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCtt                               <  7:434338/149‑1 (MQ=255)
                                                                                                              tttACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCtt                               <  4:38097/149‑1 (MQ=255)
                                                                                                              tttACTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCt                                <  6:296995/148‑1 (MQ=255)
                                                                                                                 aCTGAAGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGtt                            >  4:398333/1‑149 (MQ=255)
                                                                                                                     aaGATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATACGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTc                          <  5:382417/147‑1 (MQ=255)
                                                                                                                       gATGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCg                        >  6:36507/1‑147 (MQ=255)
                                                                                                                         tGCCATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGgaga                    <  1:305134/149‑1 (MQ=255)
                                                                                                                            cATTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCt                                            <  1:412776/122‑1 (MQ=255)
                                                                                                                             aTTAACAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGca                                                                        <  4:276509/93‑1 (MQ=255)
                                                                                                                                aaCAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGtt             >  8:286921/1‑149 (MQ=255)
                                                                                                                                aaCAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGtt             >  6:155355/1‑149 (MQ=255)
                                                                                                                                 aCAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAgat             >  7:117564/1‑146 (MQ=255)
                                                                                                                                 aCAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGtt             >  7:160642/1‑148 (MQ=255)
                                                                                                                                  cAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGAt                                                                                        >  7:432178/1‑72 (MQ=255)
                                                                                                                                  cAAGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGAt                                                                                        <  8:432178/72‑1 (MQ=255)
                                                                                                                                   aaGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGc                  >  4:76866/1‑141 (MQ=255)
                                                                                                                                   aaGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGc                  <  3:76866/141‑1 (MQ=255)
                                                                                                                                   aaGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGtt             >  7:80831/1‑146 (MQ=255)
                                                                                                                                   aaGAGAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGtt             <  8:80831/146‑1 (MQ=255)
                                                                                                                                    agagAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGc                  <  2:193778/140‑1 (MQ=255)
                                                                                                                                    agagAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGc                  >  1:193778/1‑140 (MQ=255)
                                                                                                                                    agagAAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGata         <  5:336670/149‑1 (MQ=255)
                                                                                                                                       gaAAACAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTg      <  2:197766/149‑1 (MQ=255)
                                                                                                                                         aaaCAAGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGca    <  7:176569/149‑1 (MQ=255)
                                                                                                                                           aCAAGGAAGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGcagt  <  8:433975/149‑1 (MQ=255)
                                                                                                                                             aaGGACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGcagt  >  1:398059/1‑147 (MQ=255)
                                                                                                                                                |                                                                                                                                               
ACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCAGTTGATATTGCAGT  >  NC_000913/1649574‑1649861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: