Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,667,529 T→A 100% K346* (AAA→TAA)  mlc ← glucosamine anaerobic growth regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,667,5290TA92.6% 244.9 / 6.5 76K346* (AAA→TAA) mlcglucosamine anaerobic growth regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (1/5);  new base A (34/36);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 2.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAAT  >  NC_000913/1667385‑1667668
                                                                                                                                                |                                                                                                                                           
tCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACtt                                                                                                                                         <  6:59878/149‑1 (MQ=255)
      cTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGt                                                                                                                                   >  6:281077/1‑149 (MQ=255)
         ttACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGa                                                                                                                                 <  2:270210/148‑1 (MQ=255)
             cAGTGCAGCTCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACTCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGCCCGGGAAGAGGATATCTGCAGCTTAACTTAACGGTGAGCCaa                                                                                                                            >  6:394011/1‑149 (MQ=255)
             cAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCaa                                                                                                                            >  8:106989/1‑149 (MQ=255)
                tGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCa                                                                                                                         <  5:281077/149‑1 (MQ=255)
                tGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCa                                                                                                                         <  7:156993/149‑1 (MQ=255)
                tGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCa                                                                                                                         <  4:283725/149‑1 (MQ=255)
                         tGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGt                                                                                                                                   >  4:182671/1‑130 (MQ=255)
                         tGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGt                                                                                                                                   <  3:182671/130‑1 (MQ=255)
                         tGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTAtttttt                                                                                                                >  8:447938/1‑149 (MQ=255)
                           ccATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAGCGGTGAGCCAATCAGTATTTTTtgt                                                                                                              <  6:428164/149‑1 (MQ=255)
                           ccATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTtgt                                                                                                              <  7:106989/149‑1 (MQ=255)
                           ccATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTtgt                                                                                                              >  6:214734/1‑149 (MQ=255)
                           ccATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTtg                                                                                                               <  8:242027/148‑1 (MQ=255)
                             aTCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTgg                                                                                                            <  2:62024/149‑1 (MQ=255)
                              tCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTTATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTggg                                                                                                           <  1:263186/149‑1 (MQ=255)
                              tCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTggg                                                                                                           <  7:447938/149‑1 (MQ=255)
                              tCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTggg                                                                                                           >  4:307849/1‑149 (MQ=255)
                                     cTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGTCAATCAGTATTTTTTGTGGGTtaaat                                                                                                     >  7:325118/1‑148 (MQ=255)
                                           aGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCa                                                                                              >  3:142383/1‑149 (MQ=255)
                                           aGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCa                                                                                              >  1:45630/1‑149 (MQ=255)
                                            gAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCAc                                                                                             <  4:296244/149‑1 (MQ=255)
                                               aaCTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCat                                                                                          >  3:61207/1‑149 (MQ=255)
                                                  tGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCatgat                                                                                       >  5:206968/1‑149 (MQ=255)
                                                        ctctCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTtgtg                                                                                                             <  3:345743/121‑1 (MQ=255)
                                                        ctctCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTtgtg                                                                                                             >  4:345743/1‑121 (MQ=255)
                                                        ctctCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGc                                                                                    >  4:306664/1‑146 (MQ=255)
                                                         tctcAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCttaacttaac                                                                                                                                      <  1:294871/95‑1 (MQ=255)
                                                         tctcAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCttaacttaac                                                                                                                                      >  2:294871/1‑95 (MQ=255)
                                                           tcAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGCGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAAt                                                                              >  3:5927/1‑149 (MQ=255)
                                                             aaCGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAAt                                                                              <  5:432639/147‑1 (MQ=255)
                                                             aaCGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATgc                                                                            >  1:124507/1‑149 (MQ=255)
                                                             aaCGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATgc                                                                            >  8:310454/1‑149 (MQ=255)
                                                                gCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGcc                                                                         <  2:124507/149‑1 (MQ=255)
                                                                   gATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGcttaacttaa                                                                                                                                       <  8:256922/84‑1 (MQ=255)
                                                                   gATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGcttaacttaa                                                                                                                                       >  7:256922/1‑84 (MQ=255)
                                                                    aTGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGAc                                                                     <  3:307849/149‑1 (MQ=255)
                                                                      gtgCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTtg                                                                                                               >  6:75867/1‑105 (MQ=255)
                                                                            aCTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATgcgc                                                               <  7:310454/147‑1 (MQ=255)
                                                                              tataCGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGcc                                                            >  5:359766/1‑148 (MQ=255)
                                                                                taCGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGccc                                                           <  6:359766/147‑1 (MQ=255)
                                                                                   gCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGcc                                                            >  4:19243/1‑143 (MQ=255)
                                                                                   gCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGcc                                                            <  3:19243/143‑1 (MQ=255)
                                                                                    cAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGTCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCg                                                     <  8:325118/149‑1 (MQ=255)
                                                                                       gAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGtt                                                                                                         <  4:406196/94‑1 (MQ=255)
                                                                                       gAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGtt                                                                                                         >  3:406196/1‑94 (MQ=255)
                                                                                       gAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaat                                                                                                     <  3:320834/98‑1 (MQ=255)
                                                                                       gAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaat                                                                                                     >  4:320834/1‑98 (MQ=255)
                                                                                       gAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTa                                                  <  4:5927/149‑1 (MQ=255)
                                                                                          gggCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatg                                               <  1:263365/149‑1 (MQ=255)
                                                                                            gCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaa                                                                                                      >  3:82563/1‑92 (MQ=255)
                                                                                            gCCTGCTGACGGAGGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaa                                                                                                      <  4:82563/92‑1 (MQ=255)
                                                                                               tgctgACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCgg                                                    >  1:365889/1‑139 (MQ=255)
                                                                                               tgctgACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCgg                                                    <  2:365889/139‑1 (MQ=255)
                                                                                                 ctgACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCtt                                        <  5:214734/149‑1 (MQ=255)
                                                                                                  tgACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCa                                                                                              >  5:26662/1‑94 (MQ=255)
                                                                                                  tgACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCa                                                                                              <  6:26662/94‑1 (MQ=255)
                                                                                                    aCGGATGCTGTCTGAGATGACCGGGAATATGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGGCTTTTTTGTGGGGTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTACTGATGTCtttt                                      <  1:452471/148‑1 (MQ=255)
                                                                                                    aCGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCtttt                                      <  4:169471/148‑1 (MQ=255)
                                                                                                     cGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGc                                    <  4:61207/149‑1 (MQ=255)
                                                                                                      ggATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGcc                                   <  4:158705/149‑1 (MQ=255)
                                                                                                       gATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCa                                  <  4:142383/149‑1 (MQ=255)
                                                                                                         tGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatg                                               <  5:32671/134‑1 (MQ=255)
                                                                                                         tGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatg                                               >  6:32671/1‑134 (MQ=255)
                                                                                                         tGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGt                                <  3:306664/149‑1 (MQ=255)
                                                                                                                tGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATcgc                         >  8:461548/1‑149 (MQ=255)
                                                                                                                 gagaTGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATcgcc                        >  6:105705/1‑149 (MQ=255)
                                                                                                                      gACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTtgt                                                                                                              >  1:86922/1‑58 (MQ=255)
                                                                                                                      gACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTtgt                                                                                                              <  2:86922/58‑1 (MQ=255)
                                                                                                                      gACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatga                                              >  6:373291/1‑122 (MQ=255)
                                                                                                                      gACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatga                                              <  5:373291/122‑1 (MQ=255)
                                                                                                                        ccGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCTCCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAAt                 >  3:300577/1‑149 (MQ=255)
                                                                                                                            gAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATgccg             >  6:312666/1‑149 (MQ=255)
                                                                                                                             aaGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATgccgc            <  5:105705/149‑1 (MQ=255)
                                                                                                                                  gATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCt          <  3:293262/146‑1 (MQ=255)
                                                                                                                                  gATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCt          >  4:293262/1‑146 (MQ=255)
                                                                                                                                    tatCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGaca      >  5:97456/1‑148 (MQ=255)
                                                                                                                                        tGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAAt  >  3:132421/1‑148 (MQ=255)
                                                                                                                                          ccGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAAt  <  6:206968/146‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                           
TCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAAT  >  NC_000913/1667385‑1667668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: