Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,667,774 C→A 8.8% C264F (TGC→TTC)  mlc ← glucosamine anaerobic growth regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,667,7740CA 8.8% 295.5 / 14.2 91C264F (TGC→TTC) mlcglucosamine anaerobic growth regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (40/43);  new base A (3/5);  total (43/48)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01

GCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAA  >  NC_000913/1667633‑1667918
                                                                                                                                             |                                                                                                                                                
gCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGt                                                                                                                                            <  8:162242/148‑1 (MQ=255)
 ccAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGAAGCCGTGa                                                                                                                                          <  1:399687/149‑1 (MQ=255)
  cAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGAt                                                                                                                                         >  1:286251/1‑149 (MQ=255)
       gATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTccc                                                                                                                                     <  7:417000/148‑1 (MQ=255)
       gATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGAAGCCGTGATTCCCg                                                                                                                                    <  6:175644/149‑1 (MQ=255)
        aTCGCCGCGCAATGCCGCCTGACACCATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCg                                                                                                                                    >  1:299760/1‑148 (MQ=255)
        aTCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCCGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCg                                                                                                                                    >  2:398848/1‑148 (MQ=255)
        aTCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGGTTCGAGGCAGCCGTGATTCCCGc                                                                                                                                   <  4:263427/149‑1 (MQ=255)
                 cAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGc                                                                                                                          <  7:439824/149‑1 (MQ=255)
                      ccgccTGACACAATTAGTCCACGGTTAACTGTTGTACATTTAACATCGAGATCATGGTTTGATTAAGAGGCAGCTGTTCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTAGAGGCAGCGGTGATTCCCGCAATAAGAGCGTTTc                                                                                                                     <  7:254961/149‑1 (MQ=255)
                         ccTGACACAATGAGTGCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGGAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGAAGCCGTGATTCCCGCAATAACAGCGTTTCCCa                                                                                                                  <  8:331122/149‑1 (MQ=255)
                           tGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATa                                                                                                                >  3:181885/1‑149 (MQ=255)
                               acaATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACggg                                                                                                            <  6:134448/149‑1 (MQ=255)
                               acaATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCGGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACggg                                                                                                            >  6:41194/1‑149 (MQ=255)
                               acaATGAGTCCACGGTTAACGGCTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACggg                                                                                                            >  8:107633/1‑149 (MQ=255)
                                caATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGt                                                                                                           <  5:312666/149‑1 (MQ=255)
                                 aaTGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTc                                                                                                          >  6:281448/1‑149 (MQ=255)
                                 aaTGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTc                                                                                                          >  8:38308/1‑149 (MQ=255)
                                    gAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCa                                                                                                                  >  5:164782/1‑138 (MQ=255)
                                    gAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCa                                                                                                                  <  6:164782/138‑1 (MQ=255)
                                         caaggTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCtgt                                                                                                   <  2:299760/145‑1 (MQ=255)
                                         cACGGTTAACGGTTGTCCATGTAACATCGAGCTCATTGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCTGCCGTTATTCCCGCAATATCAGCGTTTCCCATACGGGTCGACCtgt                                                                                                   <  3:278322/148‑1 (MQ=255)
                                           cGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCtgtgtg                                                                                                <  7:38308/149‑1 (MQ=255)
                                           cGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGGGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCtgtgtg                                                                                                <  4:300577/149‑1 (MQ=255)
                                             gTTAACGGCTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCtgtgtgtg                                                                                              <  7:107633/149‑1 (MQ=255)
                                              ttAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTgg                                                                                             >  2:360775/1‑149 (MQ=255)
                                                   ggTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCtgtgt                                                                                                 >  2:221898/1‑140 (MQ=255)
                                                   ggTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCtgtgt                                                                                                 <  1:221898/140‑1 (MQ=255)
                                                   ggTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTAt                                                                                        >  2:11160/1‑149 (MQ=255)
                                                   ggTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTAt                                                                                        >  4:68349/1‑149 (MQ=255)
                                                       gTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTc                                                                                                                     <  7:306312/116‑1 (MQ=255)
                                                       gTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTc                                                                                                                     >  8:306312/1‑116 (MQ=255)
                                                        tCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTcc                                                                                    <  1:11160/148‑1 (MQ=255)
                                                        tCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCa                                                                                   >  8:296856/1‑149 (MQ=255)
                                                          cATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACggg                                                                                                            <  8:142899/122‑1 (MQ=255)
                                                          cATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACggg                                                                                                            >  7:142899/1‑122 (MQ=255)
                                                            tGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCAc                                                                                  >  1:301347/1‑146 (MQ=255)
                                                            tGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCAc                                                                                  >  4:216642/1‑146 (MQ=255)
                                                            tGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACga                                                                                <  3:216642/148‑1 (MQ=255)
                                                                  aTCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGactact                                                                         >  8:347697/1‑149 (MQ=255)
                                                                     gAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGAAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTActgc                                                                       <  3:225003/148‑1 (MQ=255)
                                                                         tCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATAATGTCAACGCTGGCTATGGTATCGAGGCAGCCGTGATTCCCGCAATAACAGAGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGctgcct                                                                  >  3:149922/1‑149 (MQ=255)
                                                                           aTGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGctgcctgc                                                                >  2:159544/1‑149 (MQ=255)
                                                                           aTGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGctgcctgc                                                                <  2:301347/149‑1 (MQ=255)
                                                                           aTGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGctgcctgc                                                                >  7:31412/1‑149 (MQ=255)
                                                                           aTGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGctgcctgc                                                                <  7:347697/149‑1 (MQ=255)
                                                                           aTGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGctgcctgc                                                                <  1:360775/149‑1 (MQ=255)
                                                                           aTGGATTGATTAAGACGCAGCTGTGCCAGCGCAAGAAGACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGctgcctgc                                                                <  2:351187/149‑1 (MQ=255)
                                                                              gtttgattAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGGAATAACAGCGGTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGTCTACTGCTGCCTGCgt                                                              <  4:259944/146‑1 (MQ=255)
                                                                              gattgattAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCgtg                                                             >  6:268091/1‑149 (MQ=255)
                                                                              gattgattAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCgtg                                                             <  3:346585/149‑1 (MQ=255)
                                                                                ttgattAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCgtg                                                             >  8:130610/1‑147 (MQ=255)
                                                                                 tgattAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTAtt                                                                                       <  8:418725/120‑1 (MQ=255)
                                                                                  gattAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg                                                          <  4:181885/148‑1 (MQ=255)
                                                                                    ttAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCa                                                                                   <  7:359894/121‑1 (MQ=255)
                                                                                    ttAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCa                                                                                   >  8:359894/1‑121 (MQ=255)
                                                                                      aaGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCTATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTACCATACGGGTCGACCTGTGTGTGGCCTATGTCCACGAGAATACTGCTGCCTGCGTGTAGCAGAt                                                     >  4:431330/1‑149 (MQ=255)
                                                                                        gACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGa                                                   <  5:268091/149‑1 (MQ=255)
                                                                                        gACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGa                                                   >  2:121819/1‑149 (MQ=255)
                                                                                        gACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGa                                                   <  3:68349/149‑1 (MQ=255)
                                                                                         aCGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGAc                                                  <  5:281448/149‑1 (MQ=255)
                                                                                           gCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGAcc                                                 >  2:465760/1‑148 (MQ=255)
                                                                                            cAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCAt                                               <  1:121819/149‑1 (MQ=255)
                                                                                               cTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCgg                                            <  7:296856/149‑1 (MQ=255)
                                                                                                tgtgCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGAAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCgg                                            >  6:201644/1‑148 (MQ=255)
                                                                                                  tgCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACCGCGTTTCCCATACGGGTCGACCTGTGGGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTaa                                         <  2:286251/149‑1 (MQ=255)
                                                                                                   gCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAAt                                        >  4:409682/1‑149 (MQ=255)
                                                                                                        cTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGAcgc                                   >  3:366067/1‑149 (MQ=255)
                                                                                                           aaGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCgtgt                                                            >  4:305990/1‑121 (MQ=255)
                                                                                                           aaGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCgtgt                                                            <  3:305990/121‑1 (MQ=255)
                                                                                                           aaGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGAcgcccg                                >  7:381133/1‑149 (MQ=255)
                                                                                                           aaGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGAcgccc                                 <  1:159544/148‑1 (MQ=255)
                                                                                                                   tGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGCCCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACg                        >  4:88320/1‑149 (MQ=255)
                                                                                                                   tGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACg                        >  4:360481/1‑149 (MQ=255)
                                                                                                                   tGTCCACGCTGGCGATGGTTTCGAGGAAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGctgcctgc                                                                >  2:281464/1‑109 (MQ=255)
                                                                                                                   tGTCCACGCTGGCGATGGTTTCGAGGAAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGGGGCCTATTTCCACGAGACTACTGctgcctgc                                                                <  1:281464/109‑1 (MQ=255)
                                                                                                                     tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTc                                                                                                                     >  5:416430/1‑54 (MQ=255)
                                                                                                                     tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTc                                                                                                                     <  6:416430/54‑1 (MQ=255)
                                                                                                                     tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGtt                      >  1:290308/1‑149 (MQ=255)
                                                                                                                     tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGtt                      <  1:465760/149‑1 (MQ=255)
                                                                                                                     tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGt                       <  4:366067/148‑1 (MQ=255)
                                                                                                                           cTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATc                <  7:130610/149‑1 (MQ=255)
                                                                                                                            tGGCGATGGTTTCGAGGCAGCCGTGATTCCTGCAATAACAGCGTTTCCCATACGGGTCGACCtgtgt                                                                                                 <  1:129880/67‑1 (MQ=255)
                                                                                                                            tGGCGATGGTTTCGAGGCAGCCGTGATTCCTGCAATAACAGCGTTTCCCATACGGGTCGACCtgtgt                                                                                                 >  2:129880/1‑67 (MQ=255)
                                                                                                                              gCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCCGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGtt                      <  6:429049/140‑1 (MQ=255)
                                                                                                                              gCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCAGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGtt                      >  5:429049/1‑140 (MQ=255)
                                                                                                                                gATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACga                                                                                >  6:31169/1‑80 (MQ=255)
                                                                                                                                gATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACga                                                                                <  5:31169/80‑1 (MQ=255)
                                                                                                                                gATGGTTTCGAGGAAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATaa           >  1:279610/1‑149 (MQ=255)
                                                                                                                                    gTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAaccac       <  3:409682/149‑1 (MQ=255)
                                                                                                                                         gAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGaa  >  3:203153/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                
GCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGCTCATGGATTGATTAAGACGCAGCTGTGCCAGCTCAAGAATACTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGCAGATGACCATCGGTAATGACGCCCGCCCCCACGTTGTGATCGATAACCACCTGAA  >  NC_000913/1667633‑1667918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: