Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,731,134 G→A 7.3% R683H (CGC→CAC)  lhr → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,731,1340GA 7.3% 165.8 / 2.6 55R683H (CGC→CAC) lhrputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (23/28);  new base A (2/2);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

GATTTTCTTCATTTGCTGGCGGATGGCGCGTTCTTTTCCGGGACTATTCCCCCGTGGCTGGCAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCGGTG  >  NC_000913/1730995‑1731279
                                                                                                                                           |                                                                                                                                                 
gATTTTCTTCATTTGCTGGCGGATGGCGCGTTCTTTTCCGGGACTATTCCCCCGTGGCTGGCAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGt                                                                                                                                          <  4:316374/149‑1 (MQ=255)
 aTTTTCTTCATTTGCTGGCGGATGGCGCGTTCTTTTCCGGGACTATTCCCCCGTGGCTGGCAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTc                                                                                                                                         >  1:205979/1‑149 (MQ=255)
  ttttCTTCATTTGCTGGCGGATGGCGCGTTCTTTTCCGGGACTATTCCCCCGTGGCTGGCAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTcc                                                                                                                                        <  6:284928/149‑1 (MQ=255)
     tcttcATTTGCTGGCGGATGGCGCGTTCTTTTCCGGGACTATTCCCCCGTGGCTGGCAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTTAGGAGCAGCGCAACGCGACGGGCATCGATCCGGGGAGTCGCCATCTGGACCTc                                                                                                                                      >  8:329059/1‑148 (MQ=255)
       ttcATTTGCTGGCGGATGGCGCGTTCTTTTCCGGGACTATTCCCCCGTGGCTGGCAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGaa                                                                                                                                   >  6:203934/1‑149 (MQ=255)
        tcATTTGCTGGCGGATGGCGCGTTCTTTTCCGGGACTATTCCCCCGTGGCTGGCAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAAc                                                                                                                                  >  7:417045/1‑149 (MQ=255)
              gCTGGCGGATGGCGCGTTCTTTTCCGGGACTATTCCCCCGTGGCTGGCAGAAGAAAATACGAGCGCCAATATTCAGTGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCTAACGGTGc                                                                                                                             <  3:112262/148‑1 (MQ=255)
                   cGGCTGGCGCGTTCTTTTCAGGGACTTTTCCCCCGTGGCGGGCAGAAGCAAAAACGATCTCCACTATTCAGGGGTGGATTGAGGCGCAGCGCACCGCGACGGGCATCGTGCCGGGGAGGCGCCATCTGGTCCTCGAACGGAGCCGtgag                                                                                                                       <  7:329059/149‑2 (MQ=255)
                   cGGATGGCGCGTTCTTTTCCGGGACTATTCCCCCGTGGCTGGCAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGtgat                                                                                                                       >  3:34058/1‑149 (MQ=255)
                     gATGGCGCGTGCTTTTCCGGGACTATTCCCCCGTGGCTGGCAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGtgatga                                                                                                                     <  2:409300/149‑1 (MQ=255)
                          cgcgTTCTTTTCCGGGACTATTCCCCCGTGGCTGGCAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTg                                                                                                                >  6:306970/1‑149 (MQ=255)
                          cgcgTTCTTTTCCGGGACTATTCCCCCGTGGCTGGCAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTg                                                                                                                <  7:163023/149‑1 (MQ=255)
                                 ttttCCGGGACTATTCCCCCGTGGCTGGCAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTg                                                                                                         >  6:390703/1‑149 (MQ=255)
                                            tATTCCCCCGTGGCTGGCAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGtatta                                                                                                 >  1:465118/1‑146 (MQ=255)
                                            tATTCCCCCGTGGCTGGCAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGtatta                                                                                                 <  2:465118/146‑1 (MQ=255)
                                              ttCCCCCGTGGCTGGCAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTg                                                                                            >  6:395747/1‑149 (MQ=255)
                                                 ccccGTGGCTGGCAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTg                                                                                            >  4:261261/1‑146 (MQ=255)
                                                 ccccGTGGCTGGCAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTg                                                                                            <  3:261261/146‑1 (MQ=255)
                                                   ccGTGGCTGGCAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGAc                                                                                                           >  7:347860/1‑129 (MQ=255)
                                                   ccGTGGCTGGCAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGAc                                                                                                           <  8:347860/129‑1 (MQ=255)
                                                   cagTGGCTGCCAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCAATCTGGTCCTCGAACGGTCCCGTGATGAAATTGGTGAATGGAGTATTATTTTGCActc                                                                                       >  8:35814/3‑149 (MQ=255)
                                                        gctggcAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCt                                                                                  <  7:368512/149‑1 (MQ=255)
                                                        gctggcAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCt                                                                                  <  8:417045/149‑1 (MQ=255)
                                                            gcAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATgg                                                                              <  5:203934/149‑1 (MQ=255)
                                                              agaagaAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATgg                                                                              >  1:307706/1‑147 (MQ=255)
                                                              agaagaAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGaa                                                                            <  5:306970/149‑1 (MQ=255)
                                                               gaagaaAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATgg                                                                              >  8:9903/1‑146 (MQ=255)
                                                                  gaaAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACg                                                                        >  3:62502/1‑149 (MQ=255)
                                                                     aaTACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGt                                                                     <  4:313794/149‑1 (MQ=255)
                                                                                cAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCt                                                          >  4:21098/1‑149 (MQ=255)
                                                                                   tatTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCt                                                                                  >  7:406900/1‑122 (MQ=255)
                                                                                   tatTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCt                                                                                  <  8:406900/122‑1 (MQ=255)
                                                                                        aGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTg                                                  >  5:263960/1‑149 (MQ=255)
                                                                                         ggggTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTgg                                                 >  5:36764/1‑149 (MQ=255)
                                                                                         ggggTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTgg                                                 <  4:34058/149‑1 (MQ=255)
                                                                                         ggggTTGATTGAGGAGCAGCGCAACGCGAAGGTCATCGTTCGGGGGAGTCGCCATGTGGTCCTCGAACTGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTgg                                                 <  7:35814/149‑1 (MQ=255)
                                                                                          gggTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTgg                                                 <  2:205979/148‑1 (MQ=255)
                                                                                               gattgaGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTGGCGATTg                                           >  2:165794/1‑149 (MQ=255)
                                                                                                     ggagCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTGGCGATTGCCGGGc                                     <  6:263960/149‑1 (MQ=255)
                                                                                                         cagcGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGGGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTGGCGATTGCCGGGCGAAt                                 <  5:395747/149‑1 (MQ=255)
                                                                                                           gcgcAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTGGCGATTGCCGGGCGAATAc                               <  3:21098/149‑1 (MQ=255)
                                                                                                             gcAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACAt                             <  2:307706/149‑1 (MQ=255)
                                                                                                               aaCGCGACGTGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATgc                           <  4:62502/149‑1 (MQ=255)
                                                                                                               aaCGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCt                                                                                                                                       <  7:368232/41‑1 (MQ=255)
                                                                                                               aaCGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCt                                                                                                                                       >  8:368232/1‑41 (MQ=255)
                                                                                                               aaCGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATgc                           <  7:9903/149‑1 (MQ=255)
                                                                                                                aCGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATgcg                          >  6:209658/1‑149 (MQ=255)
                                                                                                                            tCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCt              <  5:390703/149‑1 (MQ=255)
                                                                                                                            tCGTTCCGGGGAGTCACCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATg                     <  8:259936/142‑1 (MQ=255)
                                                                                                                            tCGTTCCGGGGAGTCACCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATg                     <  8:253190/142‑1 (MQ=255)
                                                                                                                            tCGTTCCGGGGAGTCACCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATg                     >  7:259936/1‑142 (MQ=255)
                                                                                                                            tCGTTCCGGGGAGTCACCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATg                     >  7:253190/1‑142 (MQ=255)
                                                                                                                               ttCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGAc           >  2:19285/1‑149 (MQ=255)
                                                                                                                                  cGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGAcgc         <  6:36764/148‑1 (MQ=255)
                                                                                                                                        gTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGGCggtg  <  1:165794/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                 
GATTTTCTTCATTTGCTGGCGGATGGCGCGTTCTTTTCCGGGACTATTCCCCCGTGGCTGGCAGAAGAAAATACGATCGCCAATATTCAGGGGTTGATTGAGGAGCAGCGCAACGCGACGGGCATCGTTCCGGGGAGTCGCCATCTGGTCCTCGAACGGTGCCGTGATGAAATTGGTGACTGGCGTATTATTTTGCACTCTCCCTATGGAAGACGGGTGCATGAACCCTGGGCGGTGGCGATTGCCGGGCGAATACATGCGCTATGGGGCGCTGACGCGTCGGTG  >  NC_000913/1730995‑1731279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑