Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 168,660 C→T 100% Q393* (CAG→TAG)  fhuA → ferrichrome outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913168,6600CT100.0% 284.9 / ‑6.7 83Q393* (CAG→TAG) fhuAferrichrome outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (36/46);  minor base G (1/0);  total (37/46)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCCAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTACAATCCGGTGAATAC  >  NC_000913/168514‑168805
                                                                                                                                                   |                                                                                                                                                 
gCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAg                                                                                                                                                 <  3:24862/149‑1 (MQ=255)
      aTGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCa                                                                                                                                            <  8:421465/148‑1 (MQ=255)
         gTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGc                                                                                                                                        <  4:278916/149‑1 (MQ=255)
             cGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAGGGCCATTAGATGCAAGTAAATACGGGCGTTATTATTGGAAGCTGCCAAACGTATACGTTGATACCGTATTGCAGCGCAACtt                                                                                                                                   >  8:271768/1‑149 (MQ=255)
                cGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTg                                                                                                                                 <  6:201546/149‑1 (MQ=255)
                 gTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGc                                                                                                                                <  4:28160/149‑1 (MQ=255)
                     gCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACt                                                                                                                            >  3:235400/1‑149 (MQ=255)
                       tCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCa                                                                                                                                            <  6:334535/131‑1 (MQ=255)
                       tCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCa                                                                                                                                            >  5:334535/1‑131 (MQ=255)
                       tCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGttt                                                                                                                                  <  4:286064/141‑1 (MQ=255)
                       tCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGttt                                                                                                                                  >  3:286064/1‑141 (MQ=255)
                        ccGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGc                                                                                                                         >  6:407564/1‑149 (MQ=255)
                         cGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGc                                                                                                                         <  2:8741/148‑1 (MQ=255)
                           aTCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCTGATAAACGCCATTATCTGGCACGTAAATACGTCGGTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGat                                                                                                                      <  4:348642/149‑1 (MQ=255)
                           aTCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGa                                                                                                                       >  7:280781/1‑148 (MQ=255)
                           aTCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGa                                                                                                                       >  1:339502/1‑148 (MQ=255)
                              cGGCGAATGCTTACATCAAACAGTGTGCGGCATTAGCGACAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATc                                                                                                                   >  6:26948/1‑149 (MQ=255)
                              cGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATc                                                                                                                   <  8:10427/149‑1 (MQ=255)
                                gCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGa                                                                                                                 <  2:356218/149‑1 (MQ=255)
                                gCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGa                                                                                                                 >  3:6848/1‑149 (MQ=255)
                                gCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGa                                                                                                                 <  5:407564/149‑1 (MQ=255)
                                 ctattGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGTATAAAGGCCTTTATCTGGCACGTAAATCCGGCGTTTATTATGAGACGCTGCAAAACTTCTCCGTTGATACCTCGTTGCAGAGCAAGTTTGCCACTGGCTATATCGAc                                                                                                                <  8:42640/145‑1 (MQ=255)
                                 cGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGAc                                                                                                                <  5:149627/149‑1 (MQ=255)
                                 cGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGAc                                                                                                                <  8:15811/149‑1 (MQ=255)
                                  gAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGc                                                                                                                         <  5:456981/139‑1 (MQ=255)
                                  gAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGc                                                                                                                         >  6:456981/1‑139 (MQ=255)
                                       cTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACAcc                                                                                                          <  2:339502/149‑1 (MQ=255)
                                          aCAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCctg                                                                                                       >  6:48922/1‑149 (MQ=255)
                                           cAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCctgc                                                                                                      <  1:423276/149‑1 (MQ=255)
                                            aGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCctgct                                                                                                     >  7:257102/1‑149 (MQ=255)
                                               aaaCAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTg                                                                                                                           <  1:334492/124‑1 (MQ=255)
                                               aaaCAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTg                                                                                                                           >  2:334492/1‑124 (MQ=255)
                                               aaaCAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCAGTTGATAACTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCCCACCCTGCTGAc                                                                                                  >  7:406996/1‑149 (MQ=255)
                                                 tcAGTGTGCGGCATTAGCGCCTGCTGATAAAGGACATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCg                                                                                                <  8:406996/148‑1 (MQ=255)
                                                    gtgtGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGgtg                                                                                             >  6:221919/1‑149 (MQ=255)
                                                      gtgCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTc                                                                                           >  2:87198/1‑149 (MQ=255)
                                                       tgCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTc                                                                                           <  8:280781/148‑1 (MQ=255)
                                                         cGGCATTAGCGCCAGCGGATAAAGGACATTATCTGGCACGTAAATAGGTCTTTGATGATGATAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGCCCACACCCTGCTGACCGGTGTCGa                                                                                         <  2:142334/148‑1 (MQ=255)
                                                           gCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACtt                                                                                      <  8:375256/149‑1 (MQ=255)
                                                                   gcCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTtatgcgta                                                                              <  4:17976/149‑1 (MQ=255)
                                                                    ccAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGtt                                                                                                                                               <  1:158482/83‑1 (MQ=255)
                                                                    ccAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGtt                                                                                                                                               <  1:435816/83‑1 (MQ=255)
                                                                    ccAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGtt                                                                                                                                               >  2:158482/1‑83 (MQ=255)
                                                                    ccAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGtt                                                                                                                                               >  2:435816/1‑83 (MQ=255)
                                                                    ccAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTtatgcgtat                                                                             <  4:6848/149‑1 (MQ=255)
                                                                    ccAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTtatgcgtat                                                                             <  4:360013/149‑1 (MQ=255)
                                                                    ccAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCAGACCGGTGTCGACTTtatgcgtat                                                                             >  6:124962/1‑149 (MQ=255)
                                                                     cAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTtatgcgtatg                                                                            >  2:330377/1‑149 (MQ=255)
                                                                              aaGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGAc                                                                   <  4:249439/149‑1 (MQ=255)
                                                                              aaGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGAc                                                                   <  2:47287/149‑1 (MQ=255)
                                                                                ggCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACAt                                                                 <  5:26948/149‑1 (MQ=255)
                                                                                 gCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGAAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTATTGACAt                                                                 >  6:411118/1‑148 (MQ=255)
                                                                                 gCCATTATCTGGCACGTAAATACGTCGTTGATCATGAGAAGCTGCAAAAGTTCTCCGTTGATACCTAGTTGCAGAGCAAGTATGCCACTGGCGATATCCACCACACCCTGCTGAACGGTGTCGACTATATGCGTATGCGTAATGACAt                                                                 >  6:417699/1‑148 (MQ=255)
                                                                                    aTTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAAc                                                             <  4:112497/149‑1 (MQ=255)
                                                                                             cACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCtggttt                                                    <  1:87198/149‑1 (MQ=255)
                                                                                              aCGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCtggtttg                                                   >  5:95545/1‑149 (MQ=255)
                                                                                                 taatttcgtcgtTGATTATGAGAAGCTGCAAATCTTCTGCGCTGATACCTAGTTGCTGATCAAGTTTCCCACTGGCGATATCGACCAGACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATGAACGCCtgtttttgtt                                                <  2:135222/143‑1 (MQ=255)
                                                                                                   aaTACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCaga                                                                                                                                          <  5:349529/57‑1 (MQ=255)
                                                                                                   aaTACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCaga                                                                                                                                          >  6:349529/1‑57 (MQ=255)
                                                                                                   aaTACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTac                                              <  5:124962/149‑1 (MQ=255)
                                                                                                    aTACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTac                                              >  8:387541/1‑148 (MQ=255)
                                                                                                     tACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTacga                                            <  8:257102/149‑1 (MQ=255)
                                                                                                                 tgatgaGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGc                                 >  5:85281/1‑149 (MQ=255)
                                                                                                                 tgatgaGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGc                                 >  6:383395/1‑149 (MQ=255)
                                                                                                                    tgaGAAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCa                               <  6:85281/148‑1 (MQ=255)
                                                                                                                     gagaAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACt                             >  8:439793/1‑149 (MQ=255)
                                                                                                                     gagaAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTACGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACt                             >  8:419719/1‑149 (MQ=255)
                                                                                                                      agaAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACt                             <  6:95545/148‑1 (MQ=255)
                                                                                                                       gaAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTacga                                            <  1:170999/132‑1 (MQ=255)
                                                                                                                       gaAGCTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTacga                                            >  2:170999/1‑132 (MQ=255)
                                                                                                                           cTGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCaa                       >  6:332327/1‑149 (MQ=255)
                                                                                                                            tGCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAAt                      <  1:330377/149‑1 (MQ=255)
                                                                                                                             gCAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGCCGCTGTGCCACTGCTCAATc                     >  6:25869/1‑149 (MQ=255)
                                                                                                                              cAAAACTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAAt                      <  5:48922/147‑1 (MQ=255)
                                                                                                                               aaaaCTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCGATCTg                   >  4:366299/1‑149 (MQ=255)
                                                                                                                                 aaCTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTa                 <  7:387541/149‑1 (MQ=255)
                                                                                                                                 aaCTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTa                 >  3:165153/1‑149 (MQ=255)
                                                                                                                                 aaCTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTa                 <  7:439793/149‑1 (MQ=255)
                                                                                                                                 aaCTTCTCCGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTACGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTa                 <  7:419719/149‑1 (MQ=255)
                                                                                                                                         cGTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTACAATCCgg         >  1:461894/1‑149 (MQ=255)
                                                                                                                                          gTTGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTACAATCCGGt        >  2:132560/1‑149 (MQ=255)
                                                                                                                                           ttGATACCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTACAATCCGGTg       <  5:256320/149‑1 (MQ=255)
                                                                                                                                                aCCTAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTACAATCCGGTGAATAc  >  7:307809/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTCGTTGATGATGAGAAGCTGCAAAACTTCTCCGTTGATACCCAGTTGCAGAGCAAGTTTGCCACTGGCGATATCGACCACACCCTGCTGACCGGTGTCGACTTTATGCGTATGCGTAATGACATCAACGCCTGGTTTGGTTACGACGACTCTGTGCCACTGCTCAATCTGTACAATCCGGTGAATAC  >  NC_000913/168514‑168805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: