Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,737,435 A→G 6.8% intergenic (‑145/+21) ydhP ← / ← ynhF putative MFS transporter, inner membrane protein/stress response membrane

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,737,4350AG 6.8% 266.3 / 6.8 88intergenic (‑145/+21)ydhP/ynhFputative MFS transporter, inner membrane protein/stress response membrane
Reads supporting (aligned to +/- strand):  ref base A (43/39);  new base G (4/2);  total (47/41)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

CATGCCTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGAT  >  NC_000913/1737288‑1737583
                                                                                                                                                   |                                                                                                                                                    
cATGCCTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGaa                                                                                                                                                      <  3:199857/148‑1 (MQ=255)
 aTGCCTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAAcg                                                                                                                                                    >  8:368468/1‑149 (MQ=255)
 aTGCCTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAAc                                                                                                                                                     <  8:349535/148‑1 (MQ=255)
    ccTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACgcgc                                                                                                                                                 <  7:177296/149‑1 (MQ=255)
    ccTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACgcgc                                                                                                                                                 >  4:110176/1‑149 (MQ=255)
        aTCTCCACCTCTTCGCGTCATTACGCGATATTAATTAAAGTGGCGAAAGCATCACAGCAATCACAAAAAAATTAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAACCATGAAGGAGATGCGATCGAGAACGCGCTcc                                                                                                                                              >  7:185140/1‑148 (MQ=255)
          ctcCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGCGCGTTCTCCCTc                                                                                                                                           >  5:450958/1‑149 (MQ=255)
            ccACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTc                                                                                                                                           >  7:134341/1‑147 (MQ=255)
              aCCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGCGCGTTCTCCCTCgcat                                                                                                                                       <  6:450958/149‑4 (MQ=255)
              aCCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCgaga                                                                                                                                       <  6:328578/149‑1 (MQ=255)
                 tctTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGCGCGTTCTCCCTCgcatctc                                                                                                                                    >  4:5020/1‑143 (MQ=255)
                     cgcgTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGaa                                                                                                                                   <  3:265294/146‑1 (MQ=255)
                      gcgTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCa                                                                                                                               <  7:95000/149‑1 (MQ=255)
                      gcgTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCa                                                                                                                               <  2:310052/149‑1 (MQ=255)
                      gcgTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCa                                                                                                                               <  2:422910/149‑1 (MQ=255)
                       cgTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAATCACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGCGGGAGAACGCGCTCCCTCGAGAGGAAATCAg                                                                                                                              >  6:373977/1‑149 (MQ=255)
                             tACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAAAATGAAGGAGATGCGAGGGAGAACGAGCTCCCTCGAGATGAAATCAGTGCAgc                                                                                                                        >  4:111496/1‑149 (MQ=255)
                                gcgATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGcgcg                                                                                                                     >  8:92833/1‑149 (MQ=255)
                                gcgATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGcgcg                                                                                                                     >  1:247654/1‑149 (MQ=255)
                                gcgATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGcgcg                                                                                                                     <  2:415776/149‑1 (MQ=255)
                                      ttCATTATAGTGGCGAAAGCTTTACAGCAATAACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGTAGATGCGAGGGAGAACGCTCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGt                                                                                                                <  8:134341/148‑1 (MQ=255)
                                             aaGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCTCGGCAGTCAAACCCa                                                                                                        >  4:65034/1‑149 (MQ=255)
                                             aaGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCa                                                                                                        >  7:323825/1‑149 (MQ=255)
                                                    gAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTcc                                                                                                                                              <  8:376231/104‑1 (MQ=255)
                                                    gAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCgag                                                                                                                                        >  2:437334/1‑110 (MQ=255)
                                                    gAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCgag                                                                                                                                        <  1:437334/110‑1 (MQ=255)
                                                         cATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAAc                                                                                                                                                     <  8:469850/92‑1 (MQ=255)
                                                         cATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAAc                                                                                                                                                     >  7:469850/1‑92 (MQ=255)
                                                          aTGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTAc                                                                                                 >  7:161696/1‑143 (MQ=255)
                                                          aTGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTAc                                                                                                 <  8:161696/143‑1 (MQ=255)
                                                            gACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAAc                                                                                         >  5:194827/1‑149 (MQ=255)
                                                              cAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAAcc                                                                                        >  1:281322/1‑148 (MQ=255)
                                                              cAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAAc                                                                                         >  7:438666/1‑147 (MQ=255)
                                                              cAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAAc                                                                                         <  8:438666/147‑1 (MQ=255)
                                                               aGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGa                                                                                      <  4:93055/149‑1 (MQ=255)
                                                                 cAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAg                                                                                     >  5:226965/1‑148 (MQ=255)
                                                                  aaTCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACACGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGa                                                                                   <  5:126562/149‑1 (MQ=255)
                                                                      acaAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGa                                                                                >  2:434615/1‑148 (MQ=255)
                                                                        aaaaaaaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATaa                                                                             >  8:421222/1‑149 (MQ=255)
                                                                         aaaaaaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAAt                                                                            >  2:420127/1‑149 (MQ=255)
                                                                           aaaaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCa                                                                                                              <  1:436606/113‑1 (MQ=255)
                                                                           aaaaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCa                                                                                                              >  2:436606/1‑113 (MQ=255)
                                                                              aTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCgttg                                                                       <  1:420127/149‑1 (MQ=255)
                                                                              aTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCgttg                                                                       <  1:434615/149‑1 (MQ=255)
                                                                                 aaaaTAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCgttgtt                                                                     <  7:421222/148‑1 (MQ=255)
                                                                                  aaaTAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAg                                                                                                                 <  6:330717/103‑1 (MQ=255)
                                                                                  aaaTAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGCAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAg                                                                                                                 >  5:330717/1‑103 (MQ=255)
                                                                                   aaTAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTAc                                                                   >  3:108820/1‑148 (MQ=255)
                                                                                        cAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTg                                                              >  7:135828/1‑148 (MQ=255)
                                                                                         aaaaaGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGATGGAGAACGCGCTCCCTCGAGAGGAAATCAGTCCAGCGCGGCAGACAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGa                                                             >  1:66137/1‑148 (MQ=255)
                                                                                         aaaaaGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGaa                                                            <  3:65034/149‑1 (MQ=255)
                                                                                           aaaGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGaaaa                                                          <  7:368468/149‑1 (MQ=255)
                                                                                             agagAAAACACTTTTTCCTTTTTGATAACAAAGAGGAAGGAGTTGCGAGGGTGAACGCGCTCCCTCGAGATGAAATCAGTGCATCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAAtt                                                        <  2:66137/149‑1 (MQ=255)
                                                                                                gaAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATCCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATAGTTGTTACCAGTGAAAAttt                                                       >  8:361806/1‑147 (MQ=255)
                                                                                                 aaaaCACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAg                                                    >  5:130861/1‑149 (MQ=255)
                                                                                                  aaaCACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAgg                                                   <  2:247654/149‑1 (MQ=255)
                                                                                                   aaCACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCg                                                                                       >  8:378926/1‑112 (MQ=255)
                                                                                                    acacTTTTGCCATTTTTCTAACAAACAGGATGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCTCGGCGACGATCAAACCGATGACGATATTCGTTGTTACCAGTGAAAATTTAAGGTc                                                 <  8:185140/149‑1 (MQ=255)
                                                                                                    acacTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTc                                                 >  1:410096/1‑149 (MQ=255)
                                                                                                             ccATTTTGCTAACATACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCtcat                                        >  7:64475/1‑149 (MQ=255)
                                                                                                             ccATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCtcat                                        <  6:226965/149‑1 (MQ=255)
                                                                                                               aTTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACGAGTGAAAATTTAAGGTCGGTGCtcatc                                       >  2:360408/1‑148 (MQ=255)
                                                                                                                ttttGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCtcatc                                       >  7:233235/1‑147 (MQ=255)
                                                                                                                ttttGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCaa                                     <  6:194827/149‑1 (MQ=255)
                                                                                                                ttttGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGAACTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCtcatc                                       >  5:271831/1‑147 (MQ=255)
                                                                                                                       aacaaacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTtct                              >  1:228865/1‑149 (MQ=255)
                                                                                                                       aacaaacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTtct                              >  5:139921/1‑149 (MQ=255)
                                                                                                                        acaaacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTtctc                             >  2:439948/1‑149 (MQ=255)
                                                                                                                        acaaacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTtctc                             <  3:110176/149‑1 (MQ=255)
                                                                                                                        acaaacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTtct                              <  2:228865/148‑1 (MQ=255)
                                                                                                                        acaaacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTtc                               <  6:130861/147‑1 (MQ=255)
                                                                                                                          aaacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACgg                                                                                                     >  2:169726/1‑75 (MQ=255)
                                                                                                                          aaacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACgg                                                                                                     <  1:169726/75‑1 (MQ=255)
                                                                                                                           aacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCtt                          <  8:135828/149‑1 (MQ=255)
                                                                                                                            acaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCttt                         <  2:410096/149‑1 (MQ=255)
                                                                                                                               ggaaggaGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCttttt                       >  1:288103/1‑148 (MQ=255)
                                                                                                                                gaaggaGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAACCCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTtta                     <  2:281322/149‑1 (MQ=255)
                                                                                                                                   ggaGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTttatta                  <  1:439948/149‑1 (MQ=255)
                                                                                                                                       aTGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCTAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACcac              <  8:323825/149‑1 (MQ=255)
                                                                                                                                        tGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCaaa                                                                                          >  6:3724/1‑72 (MQ=255)
                                                                                                                                        tGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCaaa                                                                                          <  5:3724/72‑1 (MQ=255)
                                                                                                                                         tcGAGGGAGCGCGTTCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACcaca             >  5:297571/2‑148 (MQ=255)
                                                                                                                                          cGAGGGAGCGCGTTCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTaa                                                     <  7:234361/107‑1 (MQ=255)
                                                                                                                                          cGAGGGAGCGCGTTCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTaa                                                     >  8:234361/1‑107 (MQ=255)
                                                                                                                                            aGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCCCACaa          >  4:262836/1‑148 (MQ=255)
                                                                                                                                              ggAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACaaa         >  7:383352/1‑147 (MQ=255)
                                                                                                                                              ggAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACaaa         >  3:259246/1‑147 (MQ=255)
                                                                                                                                               gagaACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAg      <  6:60608/149‑1 (MQ=255)
                                                                                                                                               gagaACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCATTTTTTATTACCACACAAAAAg      >  2:22768/1‑149 (MQ=255)
                                                                                                                                                  aaCGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGa   <  4:108820/149‑1 (MQ=255)
                                                                                                                                                   aCGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGat  <  6:139921/149‑1 (MQ=255)
                                                                                                                                                   aCGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGa   <  7:92833/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CATGCCTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGAT  >  NC_000913/1737288‑1737583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑