Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,737,439 G→T 7.5% intergenic (‑149/+17) ydhP ← / ← ynhF putative MFS transporter, inner membrane protein/stress response membrane

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,737,4390GT 7.5% 282.1 / 7.0 85intergenic (‑149/+17)ydhP/ynhFputative MFS transporter, inner membrane protein/stress response membrane
Reads supporting (aligned to +/- strand):  ref base G (40/38);  new base T (4/3);  total (44/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CCTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGAT  >  NC_000913/1737292‑1737583
                                                                                                                                                   |                                                                                                                                                
ccTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACgcgc                                                                                                                                                 >  4:110176/1‑149 (MQ=255)
ccTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACgcgc                                                                                                                                                 <  7:177296/149‑1 (MQ=255)
    aTCTCCACCTCTTCGCGTCATTACGCGATATTAATTAAAGTGGCGAAAGCATCACAGCAATCACAAAAAAATTAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAACCATGAAGGAGATGCGATCGAGAACGCGCTcc                                                                                                                                              >  7:185140/1‑148 (MQ=255)
      ctcCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGCGCGTTCTCCCTc                                                                                                                                           >  5:450958/1‑149 (MQ=255)
        ccACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTc                                                                                                                                           >  7:134341/1‑147 (MQ=255)
          aCCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGCGCGTTCTCCCTCgcat                                                                                                                                       <  6:450958/149‑4 (MQ=255)
          aCCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCgaga                                                                                                                                       <  6:328578/149‑1 (MQ=255)
             tctTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGCGCGTTCTCCCTCgcatctc                                                                                                                                    >  4:5020/1‑143 (MQ=255)
                 cgcgTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGaa                                                                                                                                   <  3:265294/146‑1 (MQ=255)
                  gcgTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCa                                                                                                                               <  2:422910/149‑1 (MQ=255)
                  gcgTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCa                                                                                                                               <  7:95000/149‑1 (MQ=255)
                  gcgTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCa                                                                                                                               <  2:310052/149‑1 (MQ=255)
                   cgTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAATCACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGCGGGAGAACGCGCTCCCTCGAGAGGAAATCAg                                                                                                                              >  6:373977/1‑149 (MQ=255)
                         tACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAAAATGAAGGAGATGCGAGGGAGAACGAGCTCCCTCGAGATGAAATCAGTGCAgc                                                                                                                        >  4:111496/1‑149 (MQ=255)
                            gcgATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGcgcg                                                                                                                     >  8:92833/1‑149 (MQ=255)
                            gcgATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGcgcg                                                                                                                     <  2:415776/149‑1 (MQ=255)
                            gcgATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGcgcg                                                                                                                     >  1:247654/1‑149 (MQ=255)
                                  ttCATTATAGTGGCGAAAGCTTTACAGCAATAACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGTAGATGCGAGGGAGAACGCTCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGt                                                                                                                <  8:134341/148‑1 (MQ=255)
                                         aaGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCTCGGCAGTCAAACCCa                                                                                                        >  4:65034/1‑149 (MQ=255)
                                         aaGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCa                                                                                                        >  7:323825/1‑149 (MQ=255)
                                                gAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTcc                                                                                                                                              <  8:376231/104‑1 (MQ=255)
                                                gAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCgag                                                                                                                                        <  1:437334/110‑1 (MQ=255)
                                                gAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCgag                                                                                                                                        >  2:437334/1‑110 (MQ=255)
                                                      aTGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTAc                                                                                                 >  7:161696/1‑143 (MQ=255)
                                                      aTGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTAc                                                                                                 <  8:161696/143‑1 (MQ=255)
                                                        gACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAAc                                                                                         >  5:194827/1‑149 (MQ=255)
                                                          cAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAAcc                                                                                        >  1:281322/1‑148 (MQ=255)
                                                          cAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAAc                                                                                         >  7:438666/1‑147 (MQ=255)
                                                          cAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAAc                                                                                         <  8:438666/147‑1 (MQ=255)
                                                           aGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGa                                                                                      <  4:93055/149‑1 (MQ=255)
                                                             cAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAg                                                                                     >  5:226965/1‑148 (MQ=255)
                                                              aaTCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACACGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGa                                                                                   <  5:126562/149‑1 (MQ=255)
                                                                  acaAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGa                                                                                >  2:434615/1‑148 (MQ=255)
                                                                    aaaaaaaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATaa                                                                             >  8:421222/1‑149 (MQ=255)
                                                                     aaaaaaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAAt                                                                            >  2:420127/1‑149 (MQ=255)
                                                                       aaaaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCa                                                                                                              >  2:436606/1‑113 (MQ=255)
                                                                       aaaaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCa                                                                                                              <  1:436606/113‑1 (MQ=255)
                                                                          aTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCgttg                                                                       <  1:420127/149‑1 (MQ=255)
                                                                          aTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCgttg                                                                       <  1:434615/149‑1 (MQ=255)
                                                                             aaaaTAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCgttgtt                                                                     <  7:421222/148‑1 (MQ=255)
                                                                              aaaTAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAg                                                                                                                 <  6:330717/103‑1 (MQ=255)
                                                                              aaaTAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGCAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAg                                                                                                                 >  5:330717/1‑103 (MQ=255)
                                                                               aaTAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTAc                                                                   >  3:108820/1‑148 (MQ=255)
                                                                                    cAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTg                                                              >  7:135828/1‑148 (MQ=255)
                                                                                     aaaaaGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGATGGAGAACGCGCTCCCTCGAGAGGAAATCAGTCCAGCGCGGCAGACAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGa                                                             >  1:66137/1‑148 (MQ=255)
                                                                                     aaaaaGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGaa                                                            <  3:65034/149‑1 (MQ=255)
                                                                                       aaaGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGaaaa                                                          <  7:368468/149‑1 (MQ=255)
                                                                                         agagAAAACACTTTTTCCTTTTTGATAACAAAGAGGAAGGAGTTGCGAGGGTGAACGCGCTCCCTCGAGATGAAATCAGTGCATCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAAtt                                                        <  2:66137/149‑1 (MQ=255)
                                                                                            gaAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATCCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATAGTTGTTACCAGTGAAAAttt                                                       >  8:361806/1‑147 (MQ=255)
                                                                                             aaaaCACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAg                                                    >  5:130861/1‑149 (MQ=255)
                                                                                              aaaCACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAgg                                                   <  2:247654/149‑1 (MQ=255)
                                                                                               aaCACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCg                                                                                       >  8:378926/1‑112 (MQ=255)
                                                                                                acacTTTTGCCATTTTTCTAACAAACAGGATGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCTCGGCGACGATCAAACCGATGACGATATTCGTTGTTACCAGTGAAAATTTAAGGTc                                                 <  8:185140/149‑1 (MQ=255)
                                                                                                acacTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTc                                                 >  1:410096/1‑149 (MQ=255)
                                                                                                         ccATTTTGCTAACATACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCtcat                                        >  7:64475/1‑149 (MQ=255)
                                                                                                         ccATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCtcat                                        <  6:226965/149‑1 (MQ=255)
                                                                                                           aTTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACGAGTGAAAATTTAAGGTCGGTGCtcatc                                       >  2:360408/1‑148 (MQ=255)
                                                                                                            ttttGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCtcatc                                       >  7:233235/1‑147 (MQ=255)
                                                                                                            ttttGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCaa                                     <  6:194827/149‑1 (MQ=255)
                                                                                                            ttttGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGAACTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCtcatc                                       >  5:271831/1‑147 (MQ=255)
                                                                                                                   aacaaacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTtct                              >  1:228865/1‑149 (MQ=255)
                                                                                                                   aacaaacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTtct                              >  5:139921/1‑149 (MQ=255)
                                                                                                                    acaaacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTtctc                             >  2:439948/1‑149 (MQ=255)
                                                                                                                    acaaacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTtctc                             <  3:110176/149‑1 (MQ=255)
                                                                                                                    acaaacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTtct                              <  2:228865/148‑1 (MQ=255)
                                                                                                                    acaaacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTtc                               <  6:130861/147‑1 (MQ=255)
                                                                                                                      aaacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACgg                                                                                                     >  2:169726/1‑75 (MQ=255)
                                                                                                                      aaacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACgg                                                                                                     <  1:169726/75‑1 (MQ=255)
                                                                                                                       aacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCtt                          <  8:135828/149‑1 (MQ=255)
                                                                                                                        acaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCttt                         <  2:410096/149‑1 (MQ=255)
                                                                                                                           ggaaggaGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCttttt                       >  1:288103/1‑148 (MQ=255)
                                                                                                                            gaaggaGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAACCCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTtta                     <  2:281322/149‑1 (MQ=255)
                                                                                                                               ggaGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTttatta                  <  1:439948/149‑1 (MQ=255)
                                                                                                                                   aTGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCTAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACcac              <  8:323825/149‑1 (MQ=255)
                                                                                                                                    tGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCaaa                                                                                          >  6:3724/1‑72 (MQ=255)
                                                                                                                                    tGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCaaa                                                                                          <  5:3724/72‑1 (MQ=255)
                                                                                                                                     tcGAGGGAGCGCGTTCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACcaca             >  5:297571/2‑148 (MQ=255)
                                                                                                                                      cGAGGGAGCGCGTTCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTaa                                                     >  8:234361/1‑107 (MQ=255)
                                                                                                                                      cGAGGGAGCGCGTTCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTaa                                                     <  7:234361/107‑1 (MQ=255)
                                                                                                                                        aGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCCCACaa          >  4:262836/1‑148 (MQ=255)
                                                                                                                                          ggAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACaaa         >  7:383352/1‑147 (MQ=255)
                                                                                                                                          ggAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACaaa         >  3:259246/1‑147 (MQ=255)
                                                                                                                                           gagaACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAg      <  6:60608/149‑1 (MQ=255)
                                                                                                                                           gagaACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCATTTTTTATTACCACACAAAAAg      >  2:22768/1‑149 (MQ=255)
                                                                                                                                              aaCGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGa   <  4:108820/149‑1 (MQ=255)
                                                                                                                                               aCGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGat  <  6:139921/149‑1 (MQ=255)
                                                                                                                                               aCGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGa   <  7:92833/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CCTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGAT  >  NC_000913/1737292‑1737583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: